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| 1 | CYLD deletion triggers nuclear factor-κB-signaling and increases cell death resistance in murine hepatocytes显示文摘AIM:To analyze the role of CYLD for receptor-mediated cell death of murine hepatocytes in acute liver injury models.METHODS:Hepatocyte cell death in CYLD knockout mice(CYLD-/-)was analyzed by application of liver injury models for CD95-(Jo2)and tumor necrosis factor(TNF)-α-[D-Gal N/lipopolysaccharide(LPS)]induced apoptosis.Liver injury was assessed by measurement of serum transaminases and histological analysis.Apoptosis induction was quantified by cleaved PARP staining and Western blotting of activated caspases.Nuclear factor(NF)-κB,ERK,Akt and jun amino-terminal kinases signaling were assessed.Primary Hepatocytes were isolated by two step-collagenase perfusion and treated with recombinant TNF-αand with the CD95-ligand Jo2.Cell viability was analyzed by MTT-assay.RESULTS:Livers of CYLD-/-mice showed increased anti-apoptotic NF-κB signaling.In both applied liver injury models CYLD-/-mice showed a significantly reduced apoptosis sensitivity.After D-Gal N/LPS treatment CYLD-/-mice exhibited significantly lower levels of alanine aminotransferase(ALT)(295 U/L vs 859 U/L,P<0.05)and aspartate aminotransferase(AST)(560 U/L vs 1025 U/L,P<0.01).After Jo injection CYLD-/-mice showed 2-fold lower ALT(50 U/L vs 110 U/L,P<0.01)and lower AST(250 U/L vs 435 U/L,P<0.01)serumlevels compared to WT mice.In addition,isolated CYLD-/-primary murine hepatocytes(PMH)were less sensitive towards death receptor-mediated apoptosis and showed increased levels of Bcl-2,XIAP,c IAP1/2,survivin and c-FLIP expression upon TNF-and CD95-receptor triggering,respectively.Inhibition of NF-κB activation by the inhibitor of NF-κB phosphorylation inhibitor BAY 11-7085 inhibited the expression of antiapoptotic proteins and re-sensitized CYLD-/-PMH towards TNF-and CD95-receptor mediated cell death.CONCLUSION:CYLD is a central regulator of apoptotic cell death in murine hepatocytes by controlling NF-κB dependent anti-apoptotic signaling. | Toni Urbanik Bruno Christian Koehler Laura Wolpert Christin Elbner Anna-Lena Scherr Thomas Longerich Nicole Kautz Stefan Welte Nadine Hovelmeyer Dirk Jager Ari Waisman Henning Schulze-Bergkamen | 2014 | World Journal of Gastroenterology2014，20，45： | 3 |
| 2 | Novel endoscopic management of buried bumper syndrome in percutaneous endoscopic gastrostomy: The Olympus Hook Knife显示文摘Buried bumper syndrome(BBS) is an uncommon but serious complication of percutaneous endoscopic gastrostomy. It involves the internal fixation device, or 'bumper', migrating into the gastric wall and subsequent mucosal overgrowth. We described a case series of four patients with BBS treated with a novel endoscopic technique using a Hook Knife between June 2016 and February 2017. The Hook Knife is a rotating L-shaped cutting wire designed for hooking tissue and pulling it away from the gastric wall towards the lumen. The technique was successful in all four cases with no complications. Each patient was discharged on the day of treatment. The Hook Knife is a manoeuvrable, safe and effective device for endoscopic removal of buried bumpers and could avoid surgery in a high risk group of patients. To our knowledge this technique has not been described previously. We suggest that this technique should be added to the treatment algorithms for managing BBS. | Laura E Wolpert Dominic M Summers Andrew Tsang | 2017 | World Journal of Gastroenterology2017，23，35： | 2 |
| 3 | Tunneled tunica vaginalis flap is an effective technique for recurrent urethrocutaneous fistulas following tubularized incised plate urethroplasty 显示文摘 | Routh JC Wolpert JJ Reinberg Y | 2006 | J Urol2006，176，41： | 1 |
| 4 | No free lunch theorems for optimization显示文摘 | MACREADY W G | 1997 | IEEE Transaction on Evolutionary Computation1997，1，1： | 1 |
| 5 | No free lunch theorems for optimization显示文摘 | Wolpert D H Macready W G | 1997 | IEEE Transactions on Evolutionary Computation1997，1，1： | 1 |
| 6 | No free lunch theorems for optimization显示文摘 | Wolpert D H Macready W G | 1997 | IEEE Tansactions on Evolutionary Computation1997，1，1： | 1 |
| 7 | The Role Of Inertial Sensi- tivity In Motor Planning 显示文摘 | P N Sabes M I Jordan D M Wolpert | 2005 | Journal Of Neuroscience2005，，18： | 1 |
| 8 | No free lunch theorems for optimization显示文摘 | David H Wolpert William G MACREADY | 1997 | IEEE Trans on Evolutionary Computation1997，，1： | 1 |
| 9 | Computational principles of movement neuroscience 显示文摘 | WOLPERT D M GHAHRAMANI Z | 2000 | Nature Neuroscience2000，3，： | 1 |
| 10 | Congenital short QT syndrome and implantable cardioverter defibrillator treatment: inherent risk for inappropriate shock delivery显示文摘 | Schimpf R Wolpert C Bianchi F | 2003 | J Cardiovasc Electrophysiol2003，14，12： | 1 |
| 11 | Coevolutionary free lunches显示文摘 | David H Wolpert William G MACREADY | 2005 | IEEE Trans on Evolutionary Computation2005，9，6： | 1 |
| 12 | No free lunch theorems for optimization显示文摘 | Wolpert D H Macteady W G | 1997 | IEEE Transaction on Evolutionary Computation1997，1，1： | 1 |
| 13 | Further insights into the effect of quinidine in short QT syndrome caused by a mutation in HERG显示文摘 | WOLPERT C SCHIMPF R GIUSTETTO C | 2005 | J Cardiovasc Electrophysiol2005，16，1： | 1 |
| 14 | Systemicisotretinoin therapy normalizes exaggerated TLR -1 -mediated in-nate immune responses in acne patients 显示文摘 | Dispenza MC Wolpert EB Gilliland KL | 2012 | J Invest Dermatol2012，132，9： | 1 |
| 15 | No association between the WNT2 gene and autistic disorder 显示文摘 | McCoy P A Shao Y Wolpert C M | 2002 | American journal of medical genetics2002，114，1： | 1 |
| 16 | Short QT syndrome: clinical findings and diagnostic-therapeutic implications显示文摘 | GIUSTETTO C DI MONTE F WOLPERT C | 2006 | Eur Heart J2006，，： | 1 |
| 17 | VEGF activation of protein kinase C stimu- lates occludin phosphorylation and contributes to endothelial permeability显示文摘 | Harhaj NS Felinski EA Wolpert EB Stmdstrom JM Gardner TW Antonetti DA | 2006 | Invest Ophthalmol Vis Sci2006，47，11： | 1 |
| 18 | The presyrinx state a reversible myelopathic condition that may precede syringomyelia显示文摘 | WOLPERT SM | 2000 | AJNR2000，219，5： | 1 |
| 19 | Transcatheter Embolization of Hypogastric Artery Aneurysms: Lessons Learned显示文摘 | Lorraine M. Wolpert MD A. David. Drezner MD PhD Michael J. Hallisey MD James J. Gallagher MD Mary H. Windels MD | 2004 | Annals of Vascular Surgery2004，，4： | 1 |
| 20 | Estrogen exposure and bladder cancer risk in egyptian women显示文摘 | Wolpert BJ Amr S Ezzat S | 2010 | Maturitas2010，67，4： | 1 |
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