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| 1 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 2 | Decreased plasma adiponectin concentration in patients with essential hypertension显示文摘 | Adamczaka M Wiecek A Funahashib T | 2003 | Am J Hypertens2003，16，1： | 1 |
| 3 | Decreased plasma adiponectin concentration in patients with essential hypertension 显示文摘 | Adamczak M Wiecek A Funahashi T | 2003 | Am J Hypertens2003，16，1： | 1 |
| 4 | Rotavirus infections in children显示文摘 | Wiecek S Wos' H Grzybowska-Chlebowczyk U | 2008 | Med Wieku Rozwoj2008，12，2： | 1 |
| 5 | Decreased plasma adiponectin concentration in patients with essential hypertension显示文摘 | Marcin A Wiecek A Funahashi T | 2003 | Am J Hypertens2003，16，1： | 1 |
| 6 | Decreased plasma adiponectin concentration in patients with essential hypertension显示文摘 | Wiecek A Funahashi T | 2003 | Am J Hypertens2003，16，： | 1 |
| 7 | Adiponectin-an adipokine withunique metabolic properties显示文摘 | Wiecek A Adamczak M Chudek J | 2007 | Nephrol Dial Transplant2007，22，4： | 1 |
| 8 | 显示文摘 | Rieke P C Wiecek R Marsh B D | 1996 | Langmuir1996，12，： | 1 |
| 9 | The adipose tissue: a novel endocrine organ of interest to the nephrologist显示文摘 | WIECEK A KOKOT F CHUDEK J | 2002 | Nephrology Dialysis Transplantation2002，17，： | 1 |
| 10 | Does leptin play role in the pathogenesis of essential hypertension 显示文摘 | Kokot F Adamezak M Wiecek A | 1999 | Kidney Blood Press Res1999，22，3： | 1 |
| 11 | Contrast-associated nephropathy-old clinical problem and new therapeutic perspectives显示文摘 | Kolonko A Wiecek A | 1998 | Nephrol Dial Transplant1998，13，5： | 1 |
| 12 | Does leptin play a role in the pathogenesis of essential hypertension 显示文摘 | Kokot F Adamczak M Wiecek A | 1999 | Kidney Blood Press Res1999，22，： | 1 |
| 13 | Dcreased plasma adiponectin concentration in patients with essential hypertension显示文摘 | Adamczak M Wiecek A Funahashi T | 2003 | Am J Hypertens2003，16，： | 1 |
| 14 | Relationship between plasma rennin profile and leptinaemia in patients with essential hypertension 显示文摘 | Adamczak M Kokot F Wiecek AW | 2000 | J Hum nypertens2000，14，8： | 1 |
| 15 | Pathophysiological role of lep tin in patients with chronic renal failure, in kidney transplant patients, in patients with essential hypertension, and in pregnant women with preeclampsia显示文摘 | Kokot F Wiecek A Adamczak M | 1999 | Artif Organs1999，23，： | 1 |
| 16 | Quality utility: a compromise programming approach to robust design显示文摘 | WIECEK M ZHANG J | 1999 | ASME J Mech Des1999，121，2： | 1 |
| 17 | The nonselective adenosine antagonist theophylline does prevent renal dysfunction induced by radiographic contrast agents 显示文摘 | Kolonko A Wiecek A Kokot F | 1998 | J Nephrol1998，11，3： | 1 |
| 18 | Plasma leptin concentration in kid ney transplant patients during the early post-transplant period 显示文摘 | Kokot F Adamczak M Wiecek A | 1998 | Nephrol Dial Transplant1998，13，： | 1 |
| 19 | Cone characterizations of approximate solutions in real vector opti- mization 显示文摘 | Engau A Wiecek M M | 2007 | J Optim Theory Appl2007，134，： | 1 |
| 20 | Quality Utility - a Compromise Programming Approach to Robust Design显示文摘 | Chen W Wiecek M M Zhang J | 1999 | ASME Journal of Mechanical Design1999，121，： | 1 |
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