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| 1 | The First Imported Case of Monkeypox in the Mainland of China—Chongqing Municipality,China,September 16,2022显示文摘Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as approximately 60,000 cases have been reported in more than 100 nations and regions worldwide(2).Currently,many cases of monkeypox were identified in many nonendemic countries outside of Central and West Africa,and human-to-human transmission has occurred frequently,especially among men who have sex with men(MSM)presenting new clinical symptoms similar to syphilis and other sexually transmitted infections(3). | Hua Zhao Wenling Wang Li Zhao Sheng Ye Jingdong Song Roujian Lu Hua Zong Changcheng Wu Wei Huang Baoying Huang Yao Deng Ruhan A Wujuan Xie Li Qi Wenbo Xu Hua Ling Wenjie Tan | 2022 | China CDC weekly2022，4，38： | 35 |
| 2 | Respiratory Virus Multiplex RT-PCR Assay Sensitivities and Influence Factors in Hospitalized Children with Lower Respiratory Tract Infections显示文摘Multiplex RT-PCR assays have been widely used tools for detection and differentiation of a panel of respiratory viral pathogens. In this study, we evaluated the Qiagen ResPlex II V2.0 kit and explored factors influencing its sensitivity. Nasopharyngeal swab (NPS) specimens were prospectively collected from pediatric inpatients with lower respiratory tract infections at the time of admission in the Shenzhen Children's Hospital from May 2009 to April 2010. Total nucleic acids were extracted using the EZ1 system (Qiagen, Germany) and 17 respiratory viruses and genotypes including influenza A virus (FluA), FluB, parainfluenza virus 1 (PIV1), PIV2, PIV3, PIV4, respiratory syncytial virus (RSV), human metapneumovirus (hMPV), rhinoviruses (RhV), enteroviruses (EnV), human bocaviruses (hBoV), adenoviruses (AdV), four coronaviruses (229E, OC43, NL63 and HKU1), and FluA 2009 pandemic H1N1(H1N1-p) were detected and identified by the ResPlex II kit. In parallel, 16 real-time TaqMan quantitative RT-PCR assays were used to quantitatively detect each virus except for RhV. Influenza and parainfluenza viral cultures were also performed. Among the total 438 NPS specimens collected during the study period, one or more viral pathogens were detected in 274 (62.6%) and 201(45.9%) specimens by monoplex TaqMan RT-PCR and multiplex ResPlex, respectively. When results from monoplex PCR or cell culture were used as the reference standard, the multiplex PCR possessed specificities of 92.9-100.0%. The sensitivity of multiplex PCR for PIV3, hMPV, PIV1 and BoV were 73.1%, 70%, 66.7% and 55.6%, respectively, while low sensitivities (11.1%-40.0%) were observed for FluA, EnV, OC43, RSV and H1N1. Among the seven viruses/genotypes detected with higher frequencies, multiplex PCR sensitivities were correlated significantly with viral loads determined by the TaqMan RT-PCR in FluA, H1N1-p and RSV (p=0.011-0.000). The Qiagen ResPlex II multiplex RT-PCR kit possesses excellent specificity for simultaneous detection of 17 viral pathogens in NPS specimens in pediatric inpatients at the time of admission. The sensitivity of multiplex RT-PCR was influenced by viral loads, specimen process methods, primer and probe design and amplification condition. | Jikui Deng Zhuoya Ma Wenbo Huang Chengrong Li Heping Wang Yuejie Zheng Rong Zhou Yi-Wei Tang | 2013 | Virologica Sinica2013，28，2： | 14 |
| 3 | Rare earth oxides and their supported noble metals in application of environmental catalysis显示文摘Volatile organic compounds(VOCs),methane,carbon monoxide,soot,automotive exhaust,and nitrogen oxides are harmful to the atmosphere and human health.It is urgent to strictly control their emissions.Heterogeneous catalysis is an effective pathway for the removal of these pollutants,and the critical issue is the development of novel and high-performance catalysts.In this review,we briefly summarize the preparation methods,physicochemical properties,catalytic activities,and related reaction mechanisms for the above pollutants removal of the rare earth oxides,mixed rare earth oxide,rare earth oxidesupported noble metal,and mixed rare earth oxide-supported noble metal catalysts that have been investigated by our group and other researchers.It was found that catalytic performance was associated with the factors,such as specific surface area,pore structure,particle size and dispersion,adsorbed oxygen species concentration,reducibility,reactant activation ability or interaction between metal nanoparticles and support.Furthermore,we also envision the development trend of such a topic in future work. | Zhiquan Hou Wenbo Pei Xing Zhang Kunfeng Zhang Yuxi Liu Jiguang Deng Lin Jing Hongxing Dai | 2020 | Journal of Rare Earths2020，38，8： | 10 |
| 4 | Genome Characterization of the First Outbreak of COVID-19 Delta Variant B.1.617.2——Guangzhou City,Guangdong Province,China,May 2021显示文摘On May 20,2021,75-year-old female(Case A)went to a local hospital due to pharyngeal pain and low-grade fever in Liwan District,Guangzhou City,Guangdong Province.The oropharyngeal swab from Case A tested preliminarily positive for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)/coronavirus disease 2019(COVID-19)virus using a quantitative real-time reverse transcription polymerase chain reaction(RT-qPCR)method after a hospital visit. | Zhencui Li Kai Nie Kuibiao Li Yao Hu Yang Song Min Kang Meng Zhang Xiaoling Deng Jun Yuan Wenbo Xu Baisheng Li | 2021 | China CDC weekly2021，3，27： | 7 |
| 5 | Detection and Initial Response to a Type 2 Vaccine-Derived Poliovirus—Sichuan Province,China,2019显示文摘What is already known about this topic?After the type 2 strain of the live,attenuated poliovirus vaccine was withdrawn globally in 2016,any identification of a type 2 poliovirus is a Public Health Emergency of International Concern.A vaccinederived type 2 poliovirus(VDPV2)was identified in Sichuan,prompting an urgent,comprehensive investigation and response.What is added by this report?Type 2 monovalent,live,attenuated oral poliovirus vaccine(mOPV2)is being used to respond to the numerous VDPV2 outbreaks seen around the world.In contrast,the response in Sichuan used Sabin strain inactivated poliovirus(sIPV)to stop circulation of the VDPV2.In the 6 months following the vaccination response,there have been no VDPV2s detected in Sichuan,despite extensive search.What are the implications for public health practices?Further search for the VDPV2 must continue in order to determine whether transmission has been stopped.The ongoing investigation and response to the Sichuan VDPV2 is providing evidence to the Global Polio Eradication Initiative on managing VDPV2 outbreaks. | Jiushun Zhou Ning Wen Yong Zhang Qi Qi Chunxiang Fan Dongmei Yan Xiaoping Zhu Lixin Hao Shuangli Zhu Yu Liu Xiaozhen Ma Chao Ma Lei Nan Yong Chen Qianli Ma Cheng Wang Kun Deng Lei Cao Ge Shao Xianxiang Ding Hong Yang Zhijie An Lance ERodewald Aiqiang Xu Huaqing Wang Zijian Feng Zundong Yin Xianping Wu Wenbo Xu | 2020 | China CDC weekly2020，2，11： | 3 |
| 6 | Sequential activation of uterine epithelial IGF1R by stromal IGF1 and embryonic IGF2 directs normal uterine preparation for embryo implantation显示文摘Embryo implantation in both humans and rodents is initiated by the attachment of a blastocyst to the uterine epithelium.For blastocyst attachment,the uterine epithelium needs to transform at both the structural and molecular levels first,and then initiate the interaction with trophectoderm.Any perturbation during this process will result in implantation failure or long-term adverse pregnancy outcomes.Endocrine steroid hormones,which function through nuclear receptors,combine with the local molecules produced by the uteri or embryo to facilitate implantation.The insulin-like growth factor(IGF)signaling has been reported to play a vital role during pregnancy.However,its physiological function during implantation remains elusive.This study revealed that mice with conditional deletion of Igflr gene in uteri suffered from subfertility,mainly due to the disturbed uterine receptivity and abnormal embryo implantation.Mechanistically,we uncovered that in response to the nidatory estrogen on D4 of pregnancy,the epithelial IGF1R,stimulated by the stromal cell-produced IGF1,facilitated epithelial STAT3 activation to modulate the epithelial depolarity.Furthermore,embryonic derived IGF2 could activate both the epithelial ERK1/2 and STAT3 signaling through IGF1R,which was critical for the transcription of Cox2 and normal attachment reaction.In brief,our data revealed that epithelial IGF1R was sequentially activated by the uterine stromal IGF1 and embryonic IGF2 to guarantee normal epithelium differentiation during the implantation process. | Chan Zhou Meiying Lv Peike Wang Chuanhui Guo Zhangli Ni Haili Bao Yedong Tang Han Cai Jinhua Lu Wenbo Deng Xiaoyu Yang Guoliang Xia Haibin Wang Chao Wang Shuangbo Kong | 2021 | Journal of Molecular Cell Biology2021，13，9： | 2 |
| 7 | Microdefects and electron densities in NiTi shape memory alloys studied by positron annihilation显示文摘 | Hu Yifeng Deng Wen Hao Wenbo | 2006 | Trans Nonferrous Metals Soc China2006，16，6： | 1 |
| 8 | Theoretical analysis of low- temperature hot souree driven two-stage LiBr/H20 absorption refrigeration system 显示文摘 | Ma Wenbo Deng Shiming | 1996 | International Journal of Refrigeration1996，19，2： | 1 |
| 9 | Corneal subbasal nerve fiber regeneration in myopic patients after laser in situ keratomileusis显示文摘A total of 26 myopic patients(52 eyes) underwent laser in situ keratomileusis.In vivo confocal microscopy revealed that most of the regenerated corneal subbasal nerve fibers in the corneal flap originated from the stump of corneal subbasal nerve fibers outside the ablation zone and extended towards the center of the cornea in all patients.Meanwhile,new fibers were also found to directly regenerate from deep in the stroma in some cases.Approximately 94% of regenerated corneal subbasal nerve fibers(73/78 eyes) regrew vertically into the peripheral central 6-mm circle area 1 month after surgery,78%(28/36 eyes) grew into the central 3-6 mm area at 2 months,and 23% into the central 3-mm circle area at 3 months.In addition,there was no significant difference in corneal subbasal nerve fiber regenerative capacity between the basic fibroblast growth factor group and the 20%(v/v) deproteinized extract of calf blood group.The majority of corneal subbasal nerve fiber regeneration occurred from the stump of corneal subbasal nerve fibers outside the corneal flap,and the remaining growth occurred deep within the stroma. | Shijing Deng Mengmeng Wang Fengju Zhang Xuguang Sun Wenbo Hou Ning Guo | 2012 | Neural Regeneration Research2012，7，20： | 1 |
| 10 | Purification of salvianolic acid B from the crude extract of Salvia miltiorrhiza with hydrophilic organic/salt-containing aqueous two-phase system by counter-current chromatography显示文摘 | Zhi Wenbo Deng Qiuyun | | 0，，： | 1 |
| 11 | Regulatory considerations for animal studies of biomaterial products显示文摘Animal studies play a vital role in validating the concept,feasibility,safety,performance and efficacy of biomaterials products during their bench-to-clinic translation.This article aims to share regulatory considerations for animal studies of biomaterial products.After briefly emphasizing the importance of animal studies,issues of animal studies during biomaterial products’translation are discussed.Animal studies with unclear purposes,flawed design and poor reporting quality could significantly reduce the translation efficiency and create regulatory challenges.Regulatory perspectives on the purpose,principle,quality and regulatory science of animal studies are also presented.Animal studies should have clear purposes,follow principles of 3R+DQ(replacement,reduction,refinement,design and quality)and execute under an efficiently operating quality management system.With the advancement of regulatory science,National Medical Products Administration of China has been developing a series of standards and guidance documents on animal studies of medical devices.Case studies of making decisions on whether to conduct animal studies are provided in the end with drug-eluting stents as examples.In summary,animal studies of biomaterial products should pay close attention to the rationale,design and quality in order to achieve their purposes. | Maobo Cheng Wenbo Liu Jiazhen Zhang Song Zhang Zhaojun Guo Lu Liu Jiaxin Tian Xiangmei Zhang Jin Cheng Yinghui Liu Gang Deng Guobiao Gao Lei Sun | 2022 | Bioactive Materials2022，7，5： | 1 |
| 12 | A regulatory perspective on recombinant collagen-based medical devices显示文摘As a class of novel biomaterials manufactured by synthetic biology technologies,recombinant collagens are candidates for a variety of medical applications.In this article,a regulatory scientific perspective on recombinant collagens and their medical devices is presented with a focus on the definition,translation,classification and technical review.Recombinant collagens are categorized as recombinant human collagen,recombinant humanized collagen and recombinant collagen-like protein,as differentiated by specific compositions and structures.Based on their intended uses and associated risks,recombinant collagen-based medical devices are generally classified as Class Ⅱ or Ⅲ in China.The regulatory review of recombinant collagen-based medical devices aims to assess their safety and efficacy demonstrated by scientific evidences generated from preclinical and clinical evaluations.Taken together,opportunities as well as challenges for their future clinical translation of recombinant collagen-based medical devices abound,which highlights the essential role of regulatory science to provide new tools,standards,guidelines and methods to evaluate the safety and efficacy of medical products. | Wenbo Liu Hai Lin Peng Zhao Lina Xing Jie Li Zehua Wang Shan Ju XinLi Shi Yinghui Liu Gang Deng Guobiao Gao Lei Sun Xindong Zhang | 2022 | Bioactive Materials2022，7，6： | 1 |
| 13 | Purification of salvianolic acid B from the crude extract of Salvia miltiorrhiza with hydrophilic organic/salt-containing aqueous two-phase system by counter-current chromatography 显示文摘 | ZHI Wenbo DENG Qiuyun | 2006 | J Cbromatogr A2006，1116，12： | 1 |
| 14 | The measurement of internal surface characteristics of fuel nozzle orifices using the synchrotron X-ray micro CT technology显示文摘The design of fuel nozzle orifices at micrometer scales is crucial for generating desired fuel spray patterns, and consequently optimizing fuel combustion and emission in internal combustion engines. Although there have been several recent advancements in the characterization of orifice internal geometries, quantitative studies on the orifice internal wall surface characteristics are still challeges due to the lack of effective measuring methods. A new method for quantifying the internal wall surface characteristics of fuel nozzle micro-orifices is presented in this study to achieve a better understanding and prediction of spray characteristics: Firstly, by using the synchrotron X-ray micro CT technology, a three-dimensional digital model of the fuel nozzle tip was constructed. Secondly, a data post-processing technique was then applied to unfold the orifice internal wall surface to a flat base plane. Finally, the conventional surface characteristic quantification techniques can be used to evaluate the wall surface characteristics. Two diesel nozzles with identical orifice geometry design but different hydraulic grinding time were measured using this method. One nozzle was hydro-ground for 2 s while the other was not. The internal wall surfaces of the two orifices were successfully unfolded to base planes and their surface characteristics were respectively analyzed. The surface fluctuation data were perfectly reproduced by a Gaussian distribution function. The standard deviations of the distribution demonstrate the fluctuation range and the distribution of the entire surface fluctuation profiles. As an effective parameter to evaluate the hydraulic grinding process and the spray behaviors, the standard deviation was considered feasible for the analysis of the orifice internal wall surface characteristics. | LI ZhiLong ZHAO WenBo WU ZhiJun GONG HuiFeng HU ZongJie DENG Jun LI LiGuang | 2018 | Science China(Technological Sciences)2018，61，11： | 1 |
| 15 | A Review of Engine Fuel Injection Studies Using Synchrotron Radiation X-ray Imaging显示文摘Fuel spray characteristics directly determine the formation pattern and quality of the fuel/air mixture in an engine,and thus affect the combustion process.For this reason,the improvement and optimization of fuel injection systems is crucial to the development of engine technologies.The fuel jet breakup and atomization process is a complex liquid-gas two-phase turbulent flow system that has not yet been fully elucidated.Owing to the limitations of standard optical measurement techniques,the spray breakup mechanism and its interaction with the nozzle internal flow are still unclear.However,in recent years synchrotron radiation(SR)X-ray imaging technologies have been widely applied in engine fuel injection studies because of the higher energy and brilliance of third-generation SR light sources.This review provides a brief introduction to the development of SR technology and compares the critical parameters of the primary third-generation SR light sources available worldwide.The basic principles and applications of various X-ray imaging technologies with regard to nozzle internal structure measurements,visualization of in-nozzle flow characteristics and quantitative analyses of near-field spray transient dynamics are examined in detail. | Zhijun Wu Wenbo Zhao Zhilong Li Jun Deng Zongjie Hu Liguang Li | 2019 | Automotive Innovation2019，2，2： | 1 |
| 16 | Structural and functional analysis of a potent human neutralizing antibody against enterovirus A71显示文摘Enterovirus A71(EV-A71) causes major outbreaks of hand,foot,and mouth disease(HFMD) in many countries,most frequently affecting children,and a small proportion of cases may lead to death.Currently,no vaccine is available in most endemic regions,and no licenced treatments for EV-A71 infection are available.Here,we characterize a human monoclonal antibody(Hu MAb),E1,by screening a Fab antibody phage library derived from patients who recovered from EV-A71 infection.E1 exhibits strong neutralizing activity against EV-A71 virus in cells.The cryo-electron microscopy(cryo-EM) structures of the EV-A71 virion in complex with E1 Fab fragments demonstrated that E1 recognized an epitope formed by residues in the BC and HI loops of VP1.In a mouse model,E1 effectively protected against lethal EV-A71 challenge in both prophylactic and therapeutic treatment.In particular,E1 significantly reduces virus titers and muscle damage.E1 might represent a potential adjunct to EV-A71 treatment. | Zhe Chen Linlin Bao Bin Zhu Hua Fu Shuangli Zhu Tianjiao Ji Ying Xue Chuan Liu Xurong Wang Fengdi Li Qi Lv Feifei Qi Pin Yu Wei Deng Wenbo Xu Chuan Qin Hongrong Liu Qi Jin | 2022 | Science China(Life Sciences)2022，65，12： | 0 |
| 17 | Pd/silicalite-1: An highly active catalyst for the oxidative removal of toluene显示文摘Catalytic combustion is thought as an efficient and economic pathway to remove volatile organic compounds, and its critical issue is the development of high-performance catalytic materials. In this work, we used the in situ synthesis method to prepare the silicalite-1(S-1)-supported Pd nanoparticles(NPs). It is found that the as-prepared catalysts displayed a hexagonal prism morphology and a surface area of 390-440 m^(2)/g. The sample(0.28Pd/S-1-H)derived after reduction at 500°C in 10 vol% H_(2)showed the best catalytic activity for toluene combustion(T50%= 180℃ and T90%= 189℃ at a space velocity of 40,000 m L/(g·hr), turnover frequency(TOFPd) at 160℃ = 3.46 × 10^(-3)sec^(-1), and specific reaction rate at 160℃ = 63.8μmol/(gPd·sec)), with the apparent activation energy(41 k J/mol) obtained over the bestperforming 0.28Pd/S-1-H sample being much lower than those(51-70 k J/mol) obtained over the other samples(0.28Pd/S-1-A derived from calcination at 500℃ in air, 0.26Pd/S-1-im derived from the impregnation route, and 0.27Pd/ZSM-5-H prepared after reduction at 500℃ in 10 vol% H_(2)). Furthermore, the 0.28Pd/S-1-H sample possessed good thermal stability and its partial deactivation due to CO_(2) or H_(2)O introduction was reversible, but SO_(2) addition resulted in an irreversible deactivation. The possible pathways of toluene oxidation over 0.28Pd/S-1-H was toluene → p-methylbenzoquinone → maleic anhydride, benzoic acid, benzaldehyde → carbon dioxide and water. We conclude that the good dispersion of Pd NPs, high adsorption oxygen species concentration, large toluene adsorption capacity, strong acidity,and more Pd~0 species were responsible for the good catalytic performance of 0.28Pd/S-1-H. | Linke Wu Jiguang Deng Yuxi Liu Lin Jing Xiaohui Yu Xing Zhang Ruyi Gao Wenbo Pei Xiuqing Hao Ali Rastegarpanah Hongxing Dai | 2022 | Journal of Environmental Sciences2022，34，6： | 0 |
| 18 | Electrical Structure of Yarlung Zangbo Suture from Magnetotelluric Profiling of INDEPTH-MT显示文摘The INDEPTH MT results show that there are no electrical features of deep fractures along the Yarlung Zangbo River, but a large high conductivity body exists in the area between Gyangze and Rinbung. It dips northward, extends downward up to the depth of about 55 km and indicates the exposure of the possible real position of the Yarlung Zangbo suture. There are three sets of electrical gradient and distorted zones reflecting the structure of faults in the high conductivity region. These three fault belts, which dip northward and gradually converge downward to the main fault belt, and a series of south dipping faults in the north side form the exhaled structural characteristics of the Yarlung Zangbo suture. There is a close relationship between the large high conductivity body and the underground thermal state. | Tan Handong Jin Sheng Wei Wenbo Chen Leshou Deng Ming Department of Applied Geophysics, China University of Geosciences, Beijing 100083, China John Booker Nong Wu Martyn Unsworth University of Washington, WA 98195, USA Alan Jones Geological S | 2000 | Journal of Earth Science2000，19，3： | 0 |
| 19 | Maternal Determinants of Pregnancy Success显示文摘Pregnancy is a process describing the journey of in utero development of a life born from a fertilized egg called zygote.1 The process is highly conserved to escape eutherians from environmental insul. | Wenbo Deng Dong-bao Chen Haibin Wang | 2022 | Maternal-Fetal Medicine2022，4，1： | 0 |
| 20 | 一个E3连接酶-BAG蛋白分子模块调控植物免疫和广谱抗病性显示文摘在植物免疫过程中,细胞死亡的过程受到精细调控,然而目前对其调控机制尚不清楚。 | quanyuan you keran zhai donglei yang weibing yang jingni wu junzhong liu wenbo pan jianjun wang xudong zhu yikun jian jiyun liu yingying zhang yiwen deng qun li yonggen lou qi xie 何祖华 | 2017 | 科学新闻2017，0，4： | 0 |
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