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| 1 | m^6A-dependent biogenesis of circular RNAs in male germ cells显示文摘The majority of circular RNAs(circRNAs)spliced from coding genes contain open reading frames(ORFs)and thus,have protein coding potential.However,it remains unknown what regulates the biogenesis of these ORF-containing circRNAs,whether they are actually translated into proteins and what functions they play in specific physiological contexts.Here,we report that a large number of circRNAs are synthesized with increasing abundance when late pachytene spermatocytes develop into round and then elongating spermatids during murine spermatogenesis.For a subset of circRNAs,the back splicing appears to occur mostly at m^6A-enriched sites,which are usually located around the start and stop codons in linear mRNAs.Consequently,approximately a half of these male germ cell circRNAs contain large ORFs with m^6A-modified start codons in their junctions,features that have been recently shown to be associated with protein-coding potential.Hundreds of peptides encoded by the junction sequences of these circRNAs were detected using liquid chromatography coupled with mass spectrometry,suggesting that these circRNAs can indeed be translated into proteins in both developing(spermatocytes and spermatids)and mature(spermatozoa)male germ cells.The present study discovered not only a novel role of m^6A in the biogenesis of coding circRNAs,but also a potential mechanism to ensure stable and long-lasting protein production in the absence of linear mRNAs,i.e.,through production of circRNAs containing large ORFs and m^6A-modified start codons in junction sequences. | Chong Tang Yeming Xie Tian Yu Na Liu Zhuqing Wang Rebekah J.Woolsey Yunge Tang Xinzong Zhang Weibing Qin Ying Zhang Ge Song Weiwei Zheng Juan Wang Weitian Chen Xiongyi Wei Zhe Xie Rachel Klukovich Huili Zheng David R.Quilici Wei Yan | 2020 | Cell Research2020，30，3： | 12 |
| 2 | Distribution of Intra-Crustal Low Velocity Zones beneath Yunnan from Seismic Ambient Noise Tomography显示文摘Previous studies have reached consensus that low velocity zones are widespread in the crust beneath Yunnan region. However, the relationships between the low velocity zones and large faults, earthquake distribution are less investigated by available studies. By analyzing the seismic ambient noise recorded by Yunnan Seismic Networks and Tengchong volcano array, we construct a 3D crustal shear wave velocity model for the Yunnan region, which provides more details of the distribution of intra-crustal low velocity zones all over Yunnan. The distribution of low velocity zones shows different features at different depths. At shallow depths, the results are well correlated with near surface geological features. With increasing depth, the low velocity zones are gradually concentrated on the northern part of our study area, most likely reflecting variations in crustal thickness beneath the Yunnan region. The low velocity zones are truncated at depth by several large faults in Yunnan. It is interesting that most strong earthquakes(Ms≥5.0) occurred in Yunnan are distributed in low velocity zones or the transition zones between low and high velocity anomalies within the upper-to-middle crust. The crustal structure is composed of a brittle, seismically active upper-to-middle crust and a warm, aseismic lower crust. | Weibing Qin Shuangxi Zhang Mengkui Li Tengfei Wu Chaoyu Zhang | 2018 | Journal of Earth Science2018，29，6： | 2 |
| 3 | HCV 6a Prevalence in Guangdong Province Had the Origin from Vietnam and Recent Dissemination to Other Regions of China:Phylogeographic Analyses显示文摘 | Yongshui Fu Weibing Qin Hong Cao | 2012 | PLoS ONE2012，7，28： | 1 |
| 4 | Microstructure and Characterization of Capric-stearic Acid/Modified Expanded Vermiculite Thermal Storage Composites显示文摘In order to improve the thermal storage capacity of expanded vermiculite(EV) based formstable composite PCM(FS-PCM) via organic modification of EV, first, EV was modified with a sodium stearate(Na St) as surface modifier, and organic EV(OEV) with hydrophobicity and higher adsorption capacity for fatty acid was obtained. A novel capric-stearic acid eutectic(CA-SA)/OEV FS-PCM with high thermal storage capacity was then developed. OEV and CA-SA/OEV were characterized by scanning electron microscopy(SEM), X-ray diffraction(XRD), Fourier transform infrared spectroscopy(FTIR), differential scanning calorimetry(DSC), thermal gravimetry(TG), and thermal cycling test. Results showed that OEV has obvious hydrophobicity and a higher adsorption capacity for fatty acid. Its adsorption ratio has increased by 48.71% compared with that of EV. CA-SA/OEV possesses high thermal storage density(112.52 J/g), suitable melting temperature(20.49 ℃), good chemical compatibility, excellent thermal stability and reliability, indicating great application potential for building energy efficiency. Moreover, organic modification of inorganic matrix may offer novel options for improving its adsorption capacity for organic PCMs and increasing heat storage capacity of corresponding FS-PCMs. | 刘凤利 朱教群 LIU Junhua MA Baoguo ZHOU Weibing LI Ruguang QIN Weigao | 2018 | Journal of Wuhan University of Technology(Materials Science)2018，33，2： | 1 |
| 5 | Transcriptomic and metabolomic analysis provides insights into lignin biosynthesis and accumulation and differences in lodging resistance in hybrid wheat显示文摘The use of hybrid wheat is one way to improve the yield in the future.However,greater plant heights increase lodging risk to some extent.In this study,two hybrid combinations with differences in lodging resistance were used to analyze the stem-related traits during the filling stage,and to investigate the mechanism of the difference in lodging resistance by analyzing lignin synthesis of the basal second internode(BSI).The stem-related traits such as the breaking strength,stem pole substantial degree(SPSD),and rind penetration strength(RPS),as well as the lignin content of the lodging-resistant combination(LRC),were significantly higher than those of the lodgingsensitive combination(LSC).The phenylpropanoid biosynthesis pathway was significantly and simultaneously enriched according to the transcriptomics and metabolomics analysis at the later filling stage.A total of 35 critical regulatory genes involved in the phenylpropanoid pathway were identified.Moreover,42%of the identified genes were significantly and differentially expressed at the later grain-filling stage between the two combinations,among which more than 80%were strongly up-regulated at that stage in the LRC compared with LSC.On the contrary,the LRC displayed lower contents of lignin intermediate metabolites than the LSC.These results suggested that the key to the lodging resistance formation of LRC is largely the higher lignin synthesis at the later grain-filling stage.Finally,breeding strategies for synergistically improving plant height and lodging resistance of hybrid wheat were put forward by comparing the LRC with the conventional wheat applied in large areas. | Weibing Yang Shengquan Zhang Qiling Hou Jiangang Gao Hanxia Wang Xianchao Chen Xiangzheng Liao Fengting Zhang Changping Zhao Zhilie Qin | 2024 | Journal of Integrative Agriculture2024，23，4： | 0 |
| 6 | Small RNA sequencing revealed aberrant piRNA expression profiles in deciduas of recurrent spontaneous abortion patients显示文摘Piwi-interacting RNAs(piRNAs)is a novel class of non-coding RNAs.However,changes in piRNA expression profiles in recurrent spontaneous abortion(RSA)have not yet been investigated.The aim of this study was to identify differentially expressed piRNAs in deciduas of RSA patients.Decidua tissues were collected by curettage from recruited RSA patients and normal early pregnant(NEP)women with their informed consent.Small RNA sequencing was used to evaluate the differences in piRNA expression profiles between RSA and NEP.The present results demonstrated that the counts of total piRNA reads in RSA samples were increased compared with those in NEP samples(0.21%vs.0.11%).Differential expression analysis identified 29 upregulated piRNAs and 18 downregulated piRNAs in RSA samples.RT-qPCR further confirmed that the expression levels of uniq-109625,uniq-89328,uniq-50651 and uniq-4569 were decreased in 8 RSA tissues,compared with 13 NEP tissues.Otherwise,pi-22628 and uniq-173406 were increased in 8 RSA tissues.Based on GO term and KEGG pathway analysis,we speculate that these piRNAs regulate RSA by targeting extracellular matrix component pathway,cell adhesion pathway and focal adhesion pathway.PiRNAs may be involved in RSA pathogenesis by target genes function on adhesion and extracellular matrix component. | JIABAO WU XIAOHUA LIU LU HAN HUA NIE YUAN TANG YUNGE TANG GE SONG LIXIN ZHENG WEIBING QIN | 2022 | BIOCELL2022，46，4： | 0 |
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