|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 成人、儿童及孕妇特发性血小板减少性紫癜诊治指南显示文摘特发性血小板减少性紫癜(ITP)是一种自体免疫性疾病,以持续性血小板减少为特征(血小板<150×109/L).其发病机理是自身抗体与血小板抗原结合导致它们在未成熟时即被网状内皮系统,特别是在脾脏中被破坏引起血小板减少. | Provan D Newland A Norfolk D Bolton-Maggs P Lilleyman J Greer I May A Murphy M Ouwehand W Watson S 李振宇 徐开林 | 2004 | 国外医学(输血及血液学分册)2004，27，4： | 25 |
| 2 | MicroRNAs, development of Barrett’s esophagus, and progression to esophageal adenocarcinoma显示文摘Barrett's esophagus is a premalignant condition caused by gastroesophageal reflux. Once developed, it can progress through varying grades of dysplasia to esoph-ageal adenocarcinoma. Whilst it is well accepted that Barrett's esophagus is caused by gastroesophageal reflux, the molecular mechanisms of its pathogenesis and progression to cancer remain unclear. MicroRNAs (miRNAs) are short segments of RNA that have been shown to control the expression of many human genes. They have been implicated in most cellular processes, and the role of miRNAs in disease development is be-coming increasingly evident. Understanding altered miRNA expression is likely to help unravel the molecular mechanisms that underpin the development of Barrett's esophagus and its progression to cancer. | Cameron M Smith David I Watson Michael Z Michael Damian J Hussey | 2010 | World Journal of Gastroenterology2010，16，5： | 23 |
| 3 | miR-200 family expression is downregulated upon neoplastic progression of Barrett's esophagus显示文摘AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett's epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P < 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett's epithelium from gastric and duodenalepithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P < 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P < 0.02). The expression of all miR-200 members was lower in Barrett's epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett's epithelium. We observed signif icant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett's epithelium and esophageal adenocarcinoma (P < 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett's epithelium and regulate key neoplastic processes in this epithelium. | Cameron M Smith David I Watson Mary P Leong George C Mayne Michael Z Michael Bas PL Wijnhoven Damian J Hussey | 2011 | World Journal of Gastroenterology2011，17，8： | 13 |
| 4 | Variant Cizl is a circu- lating biomarker for early-stage lung cancer 显示文摘 | Higgins G Roper K M Watson I J | 2012 | PNAS2012，109，45： | 1 |
| 5 | Allelie association of gene markers on chromosomes 5q and 11q with atopy and bronchial hyperresponsiveness 显示文摘 | Doull I J Lawrence S Watson M | 1996 | Am J Respir Grit Care Med1996，153，4： | 1 |
| 6 | An examination of message-relevant affect in road safety messages:Should road safety advertisements aim to make us feel good or bad?显示文摘 | Watson I L White K M | 2008 | Transportation Research Part F2008，11，40： | 1 |
| 7 | Investigations of Barium β- Diketonate Complexes Used in Chemical Vapour Deposition of High-Tc Oxide Films 显示文摘 | Watson I M Atwood M P Haq S | 1994 | Supercond Sci Technol1994，7，： | 1 |
| 8 | Clenbuterol,a beta-adrenoceptor agonist, incerases relative muscle strength in orthopacdic patients显示文摘 | Maltin C A Delday M I Watson J S | 1993 | Clinical Science (Colch)1993，84，3： | 1 |
| 9 | Field trial of a staphylococcal mastitis vaccine in dairy herds: clinical,subclinical and microbiological assessments显示文摘 | Watson D L McColl M L Davies H I | 1996 | Aust Vet J1996，74，6： | 1 |
| 10 | Regulation of glutamine synthetase genes in leaves of Phaseolus vulgaris 显示文摘 | Cock J M Brock I W Watson A T | 1991 | Plant Mol Biol1991，17，： | 1 |
| 11 | Randomized trial of tacrolimus versus cyclosporin microemulsion in renal transplantation显示文摘 | Trompeter R Filler G Webb NJ Watson AR Milford DV Tyden G Grenda R Janda J Hughes D Ehrich JH Klare B Zacchello G Bjorn Brekke I McGraw M Perner F Ghio L Balzar E Friman S Gusmano R Stolpe J | 2002 | Pediatr Nephrol2002，17，3： | 1 |
| 12 | Mouse macrophage paraoxonase 2 (PON2)activity is increased whereas cellular PON3 activity is decreased under oxidative stress显示文摘 | ROSENBLAT M DRAGANOV D I WATSON C E | 2003 | Arterioscler Thromb Vasc Biol2003，23，： | 1 |
| 13 | Mild heat and calcium treatment effects on fresh-cut eaJ~taloupe melon during storage显示文摘 | I AMIKANRA O WATSON M A | 2007 | Food Chemistry)2007，102，： | 1 |
| 14 | A numerical method of visco-elastic stress analysis显示文摘 | Zienkiewicz O C,Watson M,King I P | 1968 | International Journal of Mechanical1968，10，10： | 1 |
| 15 | Ultrafast near-infrared sintering of a slot-die coated nano-silver conducting ink显示文摘 | Cherrington M Claypole T C Deganello D Mabbett I Watson T Worsley D | 2011 | Journal of Materials Chemistry2011，21，21： | 1 |
| 16 | Metal-organic CVD of the high-tc superconductor YBa2Cu3O7-δ显示文摘 | Watson I M | 1997 | Chem Vap Deposition1997，3，1： | 1 |
| 17 | A new approach to the determination of the major and minor constituents in silicate and phosphate rocks 显示文摘 | Brenner I B Watson A E Russell O M | 1980 | Chemical Geology1980，28，： | 1 |
| 18 | Serum outperforms plasma in small extracellular vesicle microRNA biomarker studies of adenocarcinoma of the esophagus显示文摘BACKGROUND Circulating microRNAs(miRNAs)are potential biomarkers for many diseases.However,they can originate from non-disease specific sources,such as blood cells,and compromise the investigations for miRNA biomarkers.While small extracellular vesicles(sEVs)have been suggested to provide a purer source of circulating miRNAs for biomarkers discovery,the most suitable blood sample for sEV miRNA biomarker studies has not been defined.AIM To compare the mi RNA profiles between matched serum and plasma s EV preparations to determine their suitability for biomarker studies.METHODS Matched serum and plasma samples were obtained from 10 healthy controls and10 patients with esophageal adenocarcinoma.s EV isolates were prepared from serum and plasma using Exo Quick TM and quantified using Nano Sight.RNA was extracted from s EV preparations with the mi RNeasy Serum/Plasma kit and profiled using the Taqman Openarray q PCR.The overall mi RNA content and theexpression of specific mi RNAs of reported vesicular and non-vesicular origins were compared between serum and plasma s EV preparations.The diagnostic performance of a previously identified multi-mi RNA biomarker panel for esophageal adenocarcinoma was also compared.RESULTS The overall mi RNA content was higher in plasma s EV preparations(480 mi RNAs)and contained 97.5%of the mi RNAs found in the serum s EV preparations(412 mi RNAs).The expression of commonly expressed mi RNAs was highly correlated(Spearman’s R=0.87,P<0.0001)between the plasma and serum s EV preparations,but was consistently higher in the plasma s EV preparations.Specific blood-cell mi RNAs(hsa-mi R-223-3 p,hsa-mi R-451 a,mi R-19 b-3 p,hsa-mi R-17-5 p,hsa-mi R-30 b-5 p,hsa-mi R-106 a-5 p,hsa-mi R-150-5 p and hsa-mi R-92 a-3 p)were expressed at 2.7 to 9.6 fold higher levels in the plasma s EV preparations compared to serum s EV preparations(P<0.05).In plasma s EV preparations,the percentage of protein-associated mi RNAs expressed at relatively higher levels(Ct 20-25)was greater than serum s EV preparations(50%vs 31%).While the percentage of vesicle-associated mi RNAs expressed at relatively higher levels was greater in the serum s EV preparations than plasma s EV preparations(70%vs 44%).A 5-mi RNA biomarker panel produced a higher cross validated accuracy for discriminating patients with esophageal adenocarcinoma from healthy controls using serum s EV preparations compared with plasma s EV preparations(AUROC 0.80 vs 0.54,P<0.05).CONCLUSION Although plasma s EV preparations contained more mi RNAs than serum s EV preparations,they also contained more mi RNAs from non-vesicle origins.Serum appears to be more suitable than plasma for s EV mi RNAs biomarkers studies. | Karen Chiam George C Mayne Tingting Wang David I Watson Tanya S Irvine Tim Bright Lorelle T Smith Imogen A Ball Joanne M Bowen Dorothy M Keefe Sarah K Thompson Damian J Hussey | 2020 | World Journal of Gastroenterology2020，26，20： | 1 |
| 19 | RU-486( Mifepristone) ameliorates diabetes but does not correct deficient beta-adrenergic signalling in adipocytes from mature C57BL/6J-ob/ob mice 显示文摘 | Gettys T W Watson P M Taylor I L | 1997 | Int J Obes Relat Metab Disord1997，21，8： | 1 |
| 20 | Extracellular cyclophilins contribute to the regulation of inflammatory responses显示文摘 | Arora K Gwinn W M Bower M A Watson A Okwumabua I MacDonald H R | 2005 | J Immunol2005，175，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员