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| 1 | Mettl3-/Mettl14-mediated mRNA N6-methyladenosine modulates murine spermatogenesis显示文摘精子发生在双 spermatogonial 干细胞(SSC ) 哪个生产 haploid 精子期间是一个区别过程。这个高度专业化的过程精确在 transcriptional, posttranscriptional,和翻译层次被控制。这里,我们报导那 N 6-methyladenosine (m 6 一) ,调整基因表示的一个 epitranscriptomic 标记,在精子发生期间起必要作用。我们现在的全面 m 6 从五个发展阶段的老鼠 spermatogenic 房间的 mRNA methylomes:无差别的 spermatogonia,类型 A 1 spermatogonia, preleptotene spermatocytes, pachytene/diplotene spermatocytes,和圆 spermatids。m 6 有 Vasa-Cre 的 RNA methyltransferase Mettl3 或 Mettl14 引起 m 6 SSC 的 A 和弄空。m 6 为 SSC 增长 / 区别被要求的抄本的一个弄空 dysregulates 翻译。在有 Stra8-GFPCre 的先进细菌房间的 Mettl3 和 Mettl14 的联合删除破坏精子形式,而有在先进细菌房间的 Mettl3 或 Mettl14 的单个删除的老鼠显示出正常精子发生。从双异种老鼠展览的 spermatids 损害了为精子形式是必要的 haploid 特定的基因的翻译。这研究加亮 mRNA m 6 在 germline 开发的修正,潜在地保证在精子发生的不同阶段协调了翻译。 | Zhen Lin Phillip J Hsu Xudong Xing Jianhuo Fang Zhike Lu Qin Zou Ke-Jia Zhang Xiao Zhang Yuchuan Zhou Teng Zhang Youcheng Zhang Wanlu Song Guifang Jia Xuerui Yang Chuan He Ming-Han Tong | 2017 | Cell Research2017，27，10： | 61 |
| 2 | Human health risk assessment of heavy metals in soil–vegetable system: A multi-medium analysis显示文摘 | Xingmei Liu Qiujin Song Yu Tang Wanlu Li Jianming Xu Jianjun Wu Fan Wang Philip Charles Brookes | 2013 | Science of the Total Environment2013，，： | 4 |
| 3 | Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation显示文摘Fundamental processes such as ribosomal RNA synthesis and chromatin remodeling take place in the nucleolus,which is hyperactive in fast-proliferating cells.The sophisticated regulatory mechanism underlying the dynamic nucleolar structure and functions is yet to be fully explored.The present study uncovers the mutual functional dependency between a previously uncharacterized human long non-coding RNA,which we renamed LETN,and a key nucleolar protein,NPM1.Specifically,being upregulated in multiple types of cancer,LETN resides in the nucleolus via direct binding with NPM1.LETN plays a critical role in facilitating the formation of NPM1 pentamers,which are essential building blocks of the nucleolar granular component and control the nucleolar functions.Repression of LETN or NPM1 led to similar and profound changes of the nucleolar morphology and arrest of the nucleolar functions,which led to proliferation inhibition of human cancer cells and neural progenitor cells.Interestingly,this inter-dependency between LETN and NPM1 is associated with the evolutionarily new variations of NPM1 and the coincidental emergence of LETN in higher primates.We propose that this human-specific protein–lncRNA axis renders an additional yet critical layer of regulation with high physiological relevance in both cancerous and normal developmental processes that require hyperactive nucleoli. | Xianteng Wang Xiaolin Hu Wanlu Song Hui Xu Zhengtao Xiao Rongyao Huang Qingran Bai Fan Zhang Yongzhen Chen Yu Liu Jianhuo Fang Xin Li Qin Shen Haitao Zhao Xuerui Yang | 2021 | Cell Research2021，31，6： | 3 |
| 4 | Intestinal cellular heterogeneity and disease development revealed by single-cell technology显示文摘The intestinal epithelium is responsible for food digestion and nutrient absorption and plays a critical role in hormone secretion,microorganism defense,and immune response.These functions depend on the integral single-layered intestinal epithelium,which shows diversified cell constitution and rapid self-renewal and presents powerful regen-eration plasticity after injury.Derailment of homeostasis of the intestine epithelium leads to the development of dis-eases,most commonly including enteritis and colorectal cancer.Therefore,it is important to understand the cellular characterization of the intestinal epithelium at the molecular level and the mechanisms underlying its homeostatic maintenance.Single-cell technologies allow us to gain molecular insights at the single-cell level.In this review,we summarize the single-cell RNA sequencing applications to understand intestinal cell characteristics,spatiotemporal evolution,and intestinal disease development. | Yalong Wang Wanlu Song Shicheng Yu Yuan Liu Ye-Guang Chen | 2022 | Cell Regeneration2022，11，1： | 2 |
| 5 | Human health risk assessment of heavy metals in soil–vegetable system: A multi-medium analysis显示文摘 | Xingmei Liu Qiujin Song Yu Tang Wanlu Li Jianming Xu Jianjun Wu Fan Wang Philip Charles Brookes | 2013 | Science of the Total Environment2013，，： | 1 |
| 6 | Synthesis of hollow polyaniline nano-capsules and their supercapacitor application显示文摘 | Wanlu Yang Zan Gao Ningning Song Yunya Zhang Yingchao Yang Jun Wang | 2014 | Journal of Power Sources2014，，： | 1 |
| 7 | The identification of 'hotspots' of heavy metal polltion in soil- rice systems at a regional scale in eastern China 显示文摘 | Li Wanlu Xu Binbin Song Qiujin | 2014 | Science of the Total Environment2014，472，： | 1 |
| 8 | 3D-bioprinted cholangiocarcinoma-on-a-chip model for evaluating drug responses显示文摘Cholangiocarcinoma(CCA)is characterized by heterogeneous mutations and a refractory nature.Thus,the development of a model for effective drug screening is urgently needed.As the established therapeutic testing models for CCA are often ineffective,we fabricated an enabling three-dimensional(3D)-bioprinted CCA-on-a-chip model that to a good extent resembled the multicellular microenvironment and the anatomical microstructure of the hepato-vascular-biliary system to perform high-content antitumor drug screening.Specifically,cholangiocytes,hepatocytes,and vascular endotheliocytes were employed for 3D bioprinting of the models,allowing for a high degree of spatial and tube-like microstructural control.Interestingly,it was possible to observe CCA cells attached to the surfaces of the gelatin methacryloyl(GelMA)hydrogelembedded microchannels and overgrown in a thickening manner,generating bile duct stenosis,which was expected to be analogous to the in vivo configuration.Over 4000 differentially expressed genes were detected in the CCA cells in our 3D coculture model compared to the traditional two-dimensional(2D)monoculture.Further screening revealed that the CCA cells grown in the 3D traditional model were more sensitive to the antitumoral prodrug than those in the 2D monoculture due to drug biotransformation by the neighboring functional hepatocytes.This study provides proof-of-concept validation of our bioprinted CCA-on-a-chip as a promising drug screening model for CCA treatment and paves the way for potential personalized medicine strategies for CCA patients in the future. | Qiong Liu Luis SMille Cesar Villalobos Ingrid Anaya Matthias Vostatek Sili Yi Wanlu Li Junlong Liao Huanghui Wu Yongteng Song Lize Xiong Yu Shrike Zhang | 2023 | Bio-Design and Manufacturing2023，6，4： | 0 |
| 9 | TMED10-mediated unconventional secretion of IL-33 regulates intestinal epithelium differentiation and homeostasis显示文摘Dear Editor,Unconventional protein secretion(UcPS)refers to the release of non-signal peptide-containing secretory proteins independent of the ER–Golgi trafficking.1 Although two major pathways including vesicle-dependent and-independent UcPS have been revealed,1 the physiological role of each pathway is largely unclear due to the lack of molecular details.Previously,we identified a translocation pathway termed transmembrane p24 trafficking protein 10(TMED10)-channeled UcPS(THU),which regulates vesicle-dependent UcPS pathway.2,3 In THU,TMED10 is a protein translocator key for mediating secretory cargo translocation into the ER–Golgi intermediate compartment to initiate vesicle-dependent UcPS. | Yang Wang Meimei Huang Xiangyue Mu Wanlu Song Qing Guo Min Zhang Yuan Liu Ye-Guang Chen Liang Ge | 2024 | Cell Research2024，34，3： | 0 |
| 10 | Method to Appraise Dangerous Class of Building Masonry Component Based on DC-YOLO Model显示文摘This DC-YOLO Model was designed in order to improve the efficiency for appraising dangerous class of buildings and avoid manual intervention,thereby making the appraisal results more objective.It is an automated method designed based on deep learning and target detection algorithms to appraise the dangerous class of building masonry component.Specifically,it(1)adopted K-means clustering to obtain the quantity and size of the prior boxes;(2)expanded the grid size to improve identification to small targets;(3)introduced in deformable convolution to adapt to the irregular shape of the masonry component cracks.The experimental results show that,comparing with the conventional method,the DC-YOLO model has better recognition rates for various targets to different extents,and achieves good effects in precision,recall rate and F1 value,which indicates the good performance in classifying dangerous classes of building masonry component. | Hongrui Zhang Wenxue Wei Xinguang Xiao Song Yang Wanlu Shao | 2020 | Computers, Materials & Continua2020，，4： | 0 |
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