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| 1 | Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer显示文摘MicroRNAs (miRNAs ) 玩在在各种各样的癌症的发展和前进的关键角色。不正常的 miR-9 表示仍然保持模糊,并且卵巢的癌症的变形前进上的一致都没被到达。在这研究,从生物信息学分析的结果证明 E-cadherin mRNA 的 3-UTR 被 miR-9 直接调整。酶记者试金结果证实 miR-9 能直接指向这 3-UTR。在卵巢的癌症织物的 miR-9 和 E-cadherin 表示被 qRT-PCR 确定。移植和侵略被在 SKOV3 和 A2780 愈合弯屈和 Transwell 系统试金检测。qRT-PCR 和西方的污点被执行检测上皮 ? 间充质的联系转变的 mRNA 和蛋白质。Immunofluorescence 技术被用来分析表示和 E-cadherin, N-cadherin,和 vimentin 的 subcellular 本地化。结果证明 miR-9 经常与配对的主要的相比是在变形浆液的卵巢的癌症织物的 upregulated。miR-9 的 Upregulation 能 downregulate E-cadherin 的表示但是 upregulate 间充质的标记(N-cadherin 和 vimentin ) 的表示。miR-9 的 Overexpression 能在卵巢的癌症支持房间移植和侵略,并且这些进程能有效地经由 miR-9 禁止者被禁止。因此,我们的学习证明 miR-9 可以经由指向 E-cadherin 和一条新奇潜在的治疗学的途径控制卵巢的癌症的转移支持卵巢的癌症转移。 | Bo Zhou Hongbin Xu Meng Xia Chaoyang Sun Na Li Ensong Guo Lili Guo Wanying Shan Hao Lu Yifan Wu Yuan Li Degui Yang Danhui Weng Li Meng Junbo Hu Ding Ma Gang Chen Kezhen Li | 2017 | Frontiers of Medicine2017，11，2： | 10 |
| 2 | Autologous transplantation with fewer fibers repairs large peripheral nerve defects显示文摘Peripheral nerve injury is a serious disease and its repair is challenging. A cable-style autologous graft is the gold standard for repairing long peripheral nerve defects; however, ensuring that the minimum number of transplanted nerve attains maximum therapeutic effect remains poorly understood. In this study, a rat model of common peroneal nerve defect was established by resecting a 10-mm long right common peroneal nerve. Rats receiving transplantation of the common peroneal nerve in situ were designated as the in situ graft group. Ipsilateral sural nerves(10–30 mm long) were resected to establish the one sural nerve graft group, two sural nerves cable-style nerve graft group and three sural nerves cable-style nerve graft group. Each bundle of the peroneal nerve was 10 mm long. To reduce the barrier effect due to invasion by surrounding tissue and connective-tissue overgrowth between neural stumps, small gap sleeve suture was used in both proximal and distal terminals to allow repair of the injured common peroneal nerve. At three months postoperatively, recovery of nerve function and morphology was observed using osmium tetroxide staining and functional detection. The results showed that the number of regenerated nerve fibers, common peroneal nerve function index, motor nerve conduction velocity, recovery of myodynamia, and wet weight ratios of tibialis anterior muscle were not significantly different among the one sural nerve graft group, two sural nerves cable-style nerve graft group, and three sural nerves cable-style nerve graft group. These data suggest that the repair effect achieved using one sural nerve graft with a lower number of nerve fibers is the same as that achieved using the two sural nerves cable-style nerve graft and three sural nerves cable-style nerve graft. This indicates that according to the ‘multiple amplification' phenomenon, one small nerve graft can provide a good therapeutic effect for a large peripheral nerve defect. | Jiu-xu Deng Dian-yin Zhang Ming Li Jian Weng Yu-hui Kou Pei-xun Zhang Na Han Bo Chen Xiao-feng Yin Bao-guo Jiang | 2017 | Neural Regeneration Research2017，12，12： | 8 |
| 3 | Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19: An evidence-based clinical practice guideline(updated version)显示文摘The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the cause of a rapidly spreading illness,coronavirus disease 2019(COVID-19),affecting more than seventeen million people around the world.Diagnosis and treatment guidelines for clinicians caring for patients are needed.In the early stage,we have issued'A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV)infected pneumonia(standard version)';now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline.We formed a working group of clinical experts and methodologists.The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas:chemoprophylaxis,diagnosis,treatments,and discharge management.We searched the literature for direct evidence on the management of COVID-19,and assessed its certainty generated recommendations using the Grading of Recommendations,Assessment,Development and Evaluation(GRADE)approach.Recommendations were either strong or weak,or in the form of ungraded consensus-based statement.Finally,we issued 34 statements.Among them,6 were strong recommendations for,14 were weak recommendations for,3 were weak recommendations against and 11 were ungraded consensus-based statement.They covered topics of chemoprophylaxis(including agents and Traditional Chinese Medicine(TCM)agents),diagnosis(including clinical manifestations,reverse transcription-polymerase chain reaction(RT-PCR),respiratory tract specimens,IgM and IgG antibody tests,chest computed tomography,chest X-ray,and CT features of asymptomatic infections),treatments(including lopinavirritonavir,umifenovir,favipiravir,interferon,remdesivir,combination of antiviral drugs,hydroxychloroquine/chloroquine,interleukin-6 inhibitors,interleukin-1 inhibitors,glucocorticoid,qingfei paidu decoction,lianhua qingwen granules/capsules,convalescent plasma,lung transplantation,invasive or noninvasive ventilation,and extracorporeal membrane oxygenation(ECMO)),and discharge management(including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge).We also created two figures of these recommendations for the implementation purpose.We hope these recommendations can help support healthcare workers caring for COVID-19 patients. | Ying-Hui Jin Qing-Yuan Zhan Zhi-Yong Peng Xue-Qun Ren Xun-Tao Yin Lin Cai Yu-Feng Yuan Ji-Rong Yue Xiao-Chun Zhang Qi-Wen Yang Jianguang Ji Jian Xia Yi-Rong Li Fu-Xiang Zhou Ya-Dong Gao Zhui Yu Feng Xu Ming-Li Tu Li-Ming Tan Min Yang Fang Chen Xiao-Ju Zhang Mei Zeng Yu Zhu Xin-Can Liu Jian Yang Dong-Chi Zhao Yu-Feng Ding Ning Hou Fu-Bing Wang Hao Chen Yong-Gang Zhang Wei Li Wen Chen Yue-Xian Shi Xiu-Zhi Yang Xue-Jun Wang Yan-Jun Zhong Ming-Juan Zhao Bing-Hui Li Lin-Lu Ma Hao Zi Na Wang Yun-Yun Wang Shao-Fu Yu Lu-Yao Li Qiao Huang Hong Weng Xiang-Ying Ren Li-Sha Luo Man-Ru Fan Di Huang Hong-Yang Xue Lin-Xin Yu Jin-Ping Gao Tong Deng Xian-Tao Zeng Hong-Jun Li Zhen-Shun Cheng Xiao-Mei Yao Xing-Huan Wang | 2020 | Military Medical Research2020，7，3： | 6 |
| 4 | Identification of petroleum aromatic fraction by comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry显示文摘Comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry(GC×GC-TOFMS) is commercially available in the 1990s,with the characteristics of large peak capacity,high resolution,high sensitivity,etc.However,its application to the petroleum and geological analyses is just emerging in China and overseas.In this research,the analytical method for petroleum aromatic fraction using GC×GC-TOFMS is set up,via the choice of the column system and optimization of setting parameters,such as temperature programming,modulation time,hot pulse time,flow rate of carrier gas,data acquisition rate and data processing.The results indicate that different polar compounds of aromatic fraction distribute as bands on structured GC×GC chromatogram.Within each band,homologous compounds appear as a roof-tile structure based on the number of substituent residues.The aromatic compounds are identified and characterized according to the GC×GC chromatogram and mass spectra.According to the polarity and the number of rings,aromatic compounds are spatially present on one chromatogram,which directly reflects the distribution characteristics of complex compounds of aromatic hydrocarbons.In addition,quantitative analysis is favored as some overlapped peaks on traditional GC-MS chromatogram have been separated completely on GC×GC.Some heterocyclic atom aromatic compounds at trace level can be clearly identified using this method,for polarity differences from other interfered aromatic compounds.The development of this method and chromatogram recognition offer petroleum geologists a practical example for the application performance of GC×GC-TOFMS. | WANG HuiTong WENG Na ZHANG ShuiChang ZHU GuangYou CHEN JianPing WEI CaiYun | 2010 | Chinese Science Bulletin2010，55，19： | 6 |
| 5 | Wnt5a Affects Schwann Cell Proliferation and Regeneration via Wnt/c-Jun and PTEN Signaling Pathway显示文摘 | Fei Yu Jian Weng Yu-Song Yuan Yu-Hui Kou Na Han Bao-Guo Jiang Pei-Xun Zhang | 2018 | Chinese Medical Journal2018，，21： | 3 |
| 6 | Comparison of geochemical parameters derived from comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry and conventional gas chromatography-mass spectrometry显示文摘The saturated and aromatic hydrocarbon fractions of crude oil samples have been analyzed by using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC×GC-TOFMS) and conventional gas chromatography-mass spectrometry (GC-MS). In order to investigate the consistency and discrepancy of the obtained data from the two instruments, some petroleum geochemical parameters have been compared. A comparison of 23 geochemical parameters indicates that 10 parameters are comparable from the two instruments with less than 5% deviations. Therefore, GC×GC-TOFMS is equivalent to conventional GC-MS in some geochemical parameter acquisitions. However, the other 13 parameters are discrepant, including gammacerane / αβ-hopane, Ts/Tm, 2-ethyl-naphthalene / 1-ethyl-naphthalene (ENR), (2, 6-dimethyl-naphthalene +2,7-dimethyl-naphthalene) / 1,5-dimethyl-naphthalene (DNR), etc. Furthermore, compared to GC× GC-TOFMS, some low concentration compounds could not be detected by the conventional GC-MS, which results in the missing of related geochemical data. Normally, this is caused by the limited separation power and peak capacity of the conventional GC column. Besides, the co-eluting peak integrations are also affected significantly due to the incomplete separation of the compounds. Some low concentration compounds might not be detected because of the interference from the baseline noise or from other substances. GC×GC-TOFMS prevails in compound separation against the conventional GC-MS by avoiding co-elution, which achieves more accurate and precise peak area measurement with the presence of a true baseline. So petroleum geochemical parameters obtained from the GC×GC-TOFMS are more reliable than those from the conventional GC-MS. GC×GC-TOFMS may become one of the most effective analytical tools for the oil and gas geochemical study. | WANG HuiTong WENG Na ZHANG ShuiChang ZHU GuangYou WEI CaiYun | 2011 | Science China Earth Sciences2011，54，12： | 3 |
| 7 | Impact of formation water on the generation of H2S in condensate reservoirs: a case study from the deep Ordovician in the Tazhong Uplift of the Tarim Basin, NW China显示文摘A number of condensate reservoirs with high concentrations of H_2S have been discovered in the deep dolomite reservoirs of the lower Ordovician Yingshan Formation(O_1y) in the Tazhong Uplift, where the formation water has a high p H value. In the O_1y reservoir, the concentrations of Mg^(2+)and SO_4^(2-)in the formation water are higher than those in the upper Ordovician formation.The concentration of H2 S in the condensate reservoirs and the concentration of Mg^(2+)in the formation water correlate well in the O_1y reservoirs of the Tazhong Uplift, which indicates a presumed thermochemical sulfate reduction(TSR) origin of H_2S according to the oxidation theory of contact ion-pairs(CIPs). Besides, the p H values of the formation water are positively correlated with the concentration of H_2S in the condensate reservoirs, which may indicate that high p H might be another factor to promote and maintain TSR. Oil–source correlation of biomarkers in the sulfuretted condensates indicates the Cambrian source rocks could be the origin of condensates. The formation water in the condensate reservoirs of O_1y is similar to that in the Cambrian; therefore, the TSR of sulfate-CIPs likelyoccurred in the Cambrian. High H_2S-bearing condensates are mainly located near the No. 1 Fault and NE-SW strikeslip faults, which are the major migration pathway of deep fluids in the Tazhong Uplift. The redox between sulfateCIPs and hydrocarbons is the generation mechanism of H_2S in the deep dolomite condensate reservoirs of the Tazhong Uplift. This finding should be helpful to predict the fluid properties of deep dolomite reservoirs. | Jin Su Yu Wang Xiao-Mei Wang Kun He Hai-Jun Yang Hui-Tong Wang Hua-Jian Wang Bin Zhang Ling Huang Na Weng Li-Na Bi Zhi-Hua Xiao | 2017 | Petroleum Science2017，14，3： | 2 |
| 8 | Repurposing econazole as a pharmacological autophagy inhibitor to treat pancreatic ductal adenocarcinoma显示文摘Pancreatic ductal adenocarcinoma(PDAC)is characterized by the highest mortality among carcinomas.The pathogenesis of PDAC requires elevated autophagy,inhibition of which using hydroxychloroquine has shown promise.However,current realization is impeded by its suboptimal use and unpredictable toxicity.Attempts to identify novel autophagy-modulating agents from already approved drugs offer a rapid and accessible approach.Here,using a patient-derived organoid model,we performed a comparative analysis of therapeutic responses among various antimalarial/fungal/parasitic/viral agents,through which econazole(ECON),an antifungal compound,emerged as the top candidate.Further testing in cell-line and xenograft models of PDAC validated this activity,which occurred as a direct consequence of dysfunctional autophagy.More specifically,ECON boosted autophagy initiation but blocked lysosome biogenesis.RNA sequencing analysis revealed that this autophagic induction was largely attributed to the altered expression of activation transcription factor 3(ATF3).Increased nuclear import of ATF3 and its transcriptional repression of inhibitor of differentiation-1(ID-1)led to inactivation of the AKT/mammalian target of rapamycin(m TOR)pathway,thus giving rise to autophagosome accumulation in PDAC cells.The magnitude of the increase in autophagosomes was sufficient to elicit ER stress-mediated apoptosis.Furthermore,ECON,as an autophagy inhibitor,exhibited synergistic effects with trametinib on PDAC.This study provides direct preclinical and experimental evidence for the therapeutic efficacy of ECON in PDAC treatment and reveals a mechanism whereby ECON inhibits PDAC growth. | Ningna Weng Siyuan Qin Jiayang Liu Xing Huang Jingwen Jiang Li Zhou Zhe Zhang Na Xie Kui Wang Ping Jin Maochao Luo Liyuan Peng Edouard C.Nice Ajay Goel Suxia Han Canhua Huang Qing Zhu | 2022 | Acta Pharmaceutica Sinica B2022，12，7： | 2 |
| 9 | Prevalence and risk factors of enteral nutrition intolerance in intensive care unit patients: a retrospective study显示文摘Background: Feeding intolerance (FI) among intensive care unit (ICU) patients undergoing early continuous enteral nutrition (EN) is related to poor outcomes. This study aimed to explore the prevalence and risk factors of FI in ICU patients.Methods: We retrospectively enrolled 1057 patients who received early continuous EN via a nasogastric tube between January 2014 and August 2019. The prevalence of FI during the first 7 days of ICU stay was calculated, and the risk factors were investigated using multivariate logistic regression analysis.Results: The prevalence of FI during the first 7 days of ICU stay was 10.95%. FI occurred in 159 of 1057 (15.04%) patients on ICU day 2, 114 of 977 (11.67%) patients on ICU day 3, and 86 of 715 (12.03%) patients on ICU day 7. Mechanical ventilation (MV) (odds ratio [OR]: 1.928, 95% confidence interval [CI]: 1.064–3.493,P = 0.03) was an independent risk factor for FI defined by a gastric residual volume (GRV) of 200 mL and/or vomiting, and acute renal failure (OR: 3.445, 95% CI: 1.115–10.707,P = 0.032) was an independent risk factor of FI defined by a GRV of 500 mL and/or vomiting. Continuous renal replacement therapy (CRRT) was an independent predictor regardless of the FI defined by a GRV of 200 mL (OR: 2.064, 95% CI: 1.233–3.456,P = 0.006) or 500 mL (OR: 6.199, 95% CI: 2.108–18.228,P = 0.001) in the ICU patients.Conclusions: FI occurs frequently in early ICU days, especially in patients receiving MV and CRRT. However, further investigation of a consensus definition of FI and risk factors is still warranted in future studies. | Kunrong Yu Na Guo Dingding Zhang Ying Xia Yanling Meng Li Weng Bin Du | 2022 | Chinese Medical Journal2022，，15： | 2 |
| 10 | Soil Organic Carbon Stocks of Citrus Orchards in Yongchun County Fujian Province China显示文摘Studies related to the quantitation and distribution of soil organic carbon(C) under different land use types can help to fill the knowledge gaps regarding estimation of the amount of C stored in soils at a global scale. Orchards are an important land use type in southern China; the total area(1.15 × 10~7 ha) of orchards in China comprises approximately 20.5% of the area of all orchards worldwide. We assessed soil organic C stocks(SOCS) in citrus orchards in Yongchun County(consisting of 22 towns), Fujian Province,China in 1982 and 2010. The southeastern part of the county, an area featuring extensive citrus farming with a gently sloping landscape and low rates of water erosion, had the highest SOCS. In contrast, the lowest SCOS was observed in the northern part of the county,where steep hills with severe erosion problems are common and citrus orchards are sparsely distributed. From 1982 to 2010, the mean SOCS in citrus orchards increased from 22.1 to 41.7 Mg ha^(-1), which indicated that current management practices such as fertilization,irrigation, and cultivation enhanced soil C stocks. Further in-depth studies on the effects of these practices on C sequestration would be worthwhile and surveys of SOCS throughout Fujian Province are needed, to support efforts to mitigate global C emission. | WANG Yixiang WENG Boqi TIAN Na ZHONG Zhenmei WANG Mingkuang | 2017 | Pedosphere2017，27，5： | 1 |
| 11 | Influ- ence detection between blog posts through blog features, content analy- sis, and conmaunity identity 显示文摘 | Tan Luke Kien - Weng Na Jin - Cheon Theng Yin - Leng | 2011 | Online infomlation review2011，35，3： | 1 |
| 12 | A new analytical method for quantifying of sterane and hopane biomarkers显示文摘An analytical method for quantifying biomarker compounds of the sterane and the hopane existing in saturated hydrocarbons has been established by using comprehensive two-dimensional gas chromatography-flame ionization detector(GC×GC-FID) with optimized operating parameters. The new method achieves the quantification by using a GC×GC-FID system which is able to completely separate steranes from hopanes. The data obtained by the new method are of good repeatability and reliability. Compared with the original data, the relative standard deviations(RSDs) of 12 reference compounds are less than 5%. The RSDs of the quantitative results of the biomarkers based on seven separate analyses are also less than 5%. Compared with the traditional method of gas chromatography-mass spectrometry(GC-MS), the new method has a number of advantages, such as common internal standards(ISs), high resolution, no co-eluting peak, and no interference caused by diagnostic ion peaks. The new method provides petroleum geologists with an effective and scientific means in future researches. | WANG HuiTong ZHANG ShuiChang WENG Na ZHANG Bin WEI XiaoFang YU Han WEI CaiYun | 2014 | Science China Earth Sciences2014，57，8： | 1 |
| 13 | Insight of unresolved complex mixtures of saturated hydrocarbons in heavy oil via GC×GC-TOFMS analysis显示文摘The saturated hydrocarbon fraction of a heavily biodegraded crude oil from Liaohe oilfield was analyzed by using comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC×GC-TOFMS). The 'humps', which was termed as unresolved complex mixtures (UCMs) in conventional gas chromatographic analysis, was separated for identification of the individual compound. Main compounds of UCMs were identified according to the combined information of characteristics of GC×GC chromatogram and time-of-flight mass spectra. It's found that UCMs are mainly composed of great number of ring compounds with extremely low concentration. The ring compounds are separated according to their boiling point diversity in the first dimension column, then to their polarity or ring number variety in the second dimension column. In the sample there are two groups of UCMs, i.e., the first group compounds with boiling point lower that of C24 and the second group compounds with boiling point higher than that of C24. The first group of UCMs mainly consists of monocyclic, bicyclic and tricyclic ring compounds. The first group of UCMs is mainly composed of saturated hydrocarbons, in which the percentage of ring compounds with signal-to-noise ratio above 100 is about 75% of the total quantity of saturated hydrocarbon, while its mass is over 80% of the total saturated hydrocarbon. The second group of UCMs is mainly composed of tetracyclic and pentacyclic ring compounds, in which the percentage of UCM compounds with signal-to-noise ratio over 100 is about 17% of the total quantity of saturated hydrocarbon, and its mass is about 0.5% of the total saturated hydrocarbon. The results of this study are helpful for heavy oil developing and genesis mechanism understanding. | WANG HuiTong ZHANG ShuiChang WENG Na WEI XiaoFang ZHU GuangYou YU Han BI LiNa MA WenLing | 2013 | Science China Chemistry2013，56，2： | 0 |
| 14 | Space advanced technology demonstration satellite显示文摘The Space Advanced Technology demonstration satellite(SATech-01),a mission for low-cost space science and new technology experiments,organized by Chinese Academy of Sciences(CAS),was successfully launched into a Sun-synchronous orbit at an altitude of~500 km on July 27,2022,from the Jiuquan Satellite Launch Centre.Serving as an experimental platform for space science exploration and the demonstration of advanced common technologies in orbit,SATech-01 is equipped with 16 experimental payloads,including the solar upper transition region imager(SUTRI),the lobster eye imager for astronomy(LEIA),the high energy burst searcher(HEBS),and a High Precision Magnetic Field Measurement System based on a CPT Magnetometer(CPT).It also incorporates an imager with freeform optics,an integrated thermal imaging sensor,and a multi-functional integrated imager,etc.This paper provides an overview of SATech-01,including a technical description of the satellite and its scientific payloads,along with their on-orbit performance. | ZHANG XiaoFeng CHEN Wen ZHU XiaoCheng MENG Na HE JunWang BI XingZi ZHANG YongHe SHI Qi LI Fei LIU Rui FENG ZhengGong LIU Liu LI JinSong WU HaiChen XU DongXiao LI TaiJie HUANG JiangJiang LIU Shuo LI TianTong YU XianSheng GAO Yang ZHOU Heng BAN HanYu ZHANG YanLi ZHANG YueTing YANG YingQuan HE Tao DUAN XuLiang CHEN Xin WANG YaMin SUN AnTai ZHANG KuoXiang SUN Ying WANG YaoBin FAN ChengCheng XIONG ShaoLin LI XinQiao WEN XiangYang LING ZhiXing SUN XiaoJin ZHANG Chen BAI XianYong WANG ZhanShan DENG YuanYong TIAN Hui YANG JianFeng XUE HongBo SANG Peng LIU JinGuo ZHENG HuiLong ZHU Xiang HE JianWu LI Hui XU LuXiang XU ShuYan CHEN WenWu LIU ZhenDong WANG ZhaoLi MAO XiangLong GAO Rong LI ZongXuan DING GuoPeng WANG XinYu DOU RunJiang WENG LuBin LUO Hao WANG YaPing LIANG XianFeng FANG ZiRuo | 2024 | Science China(Technological Sciences)2024，67，1： | 0 |
| 15 | Territory maximization hypothesis during peripheral nerve regeneration显示文摘Territory awareness refers to the notion that an organism lives in a territory,considers this territory its own,and prevents entry of other organisms.Generally,an organism maximizes its territory for best survival advantages,which subsequently allows for species continuation.Axonal sprouting occurs | jiu-xu deng jian weng yu-hui kou pei-xun zhang yan-hua wang na han bao-guo jiang xiao-feng yin | 2018 | Neural Regeneration Research2018，13，2： | 0 |
| 16 | Structural insights into ligand recognition and selectivity of somatostatin receptors显示文摘Somatostatin receptors(SSTRs)play versatile roles in inhibiting the secretion of multiple hormones such as growth hormone and thyroid-stimulating hormone,and thus are considered as targets for treating multiple tumors.Despite great progress made in therapeutic development against this diverse receptor family,drugs that target SSTRs still show limited efficacy with preferential binding affinity and conspicuous side-effects.Here,we report five structures of SSTR2 and SSTR4 in different states,including two crystal structures of SSTR2 in complex with a selective peptide antagonist and a non-peptide agonist,respectively,a cryo-electron microscopy(cryo-EM)structure of Gi1-bound SSTR2 in the presence of the endogenous ligand SST-14,as well as two cryo-EM structures of Gi1-bound SSTR4 in complex with SST-14 and a small-molecule agonist J-2156,respectively.By comparison of the SSTR structures in different states,molecular mechanisms of agonism and antagonism were illustrated.Together with computational and functional analyses,the key determinants responsible for ligand recognition and selectivity of different SSTR subtypes and multiform binding modes of peptide and non-peptide ligands were identified.Insights gained in this study will help uncover ligand selectivity of various SSTRs and accelerate the development of new molecules with better efficacy by targeting SSTRs. | Wenli Zhao Shuo Han Na Qiu Wenbo Feng Mengjie Lu Wenru Zhang Mu Wang Qingtong Zhou Shutian Chen Wei Xu Juan Du Xiaojing Chu Cuiying Yi Antao Dai Liaoyuan Hu Michelle YShen Yaping Sun Qing Zhang Yingli Ma Wenge Zhong Dehua Yang Ming-Wei Wang Beili Wu Qiang Zhao | 2022 | Cell Research2022，32，8： | 0 |
| 17 | Comparative Neonatal Outcomes of Vitrified Versus Fresh Embryo Transfers: A Systematic Review and Meta-Analysis显示文摘Objective:Vitrification prevents ice formation within the high concentration of cryoprotectant agents and allows the cells and extracellular milieu to solidify into a glass-like state.This study aimed to provide objective information on the available data regarding singleton births comparing embryo vitrification with fresh embryo transfers.Methods:PubMed and Cochrane databases were searched for studies conducted up to 2017.Low birth weight(LBW),very low birth weight(VLBW),preterm birth(PTB),very PTB,small for gestational age(SGA),large for gestational age,perinatal mortality,cesarean section,and preeclampsia were measured.Results:The search returned 220 articles,and 13 of these met the inclusion criteria.A significantly lower risk of SGA birth was observed in the vitrified embryo transfer group than in the fresh transfer group.Conversely,a higher risk of very PTB,VLBW,large size for gestational age,and perinatal mortality was observed more frequently in the vitrified embryo transfer group.Conclusions:Vitrified embryo transfer was associated with the improved outcome of neonates regarding the SGA rate compared with fresh embryo transfer,but included a higher risk of very PTB,VLBW,large size for gestational age,and perinatal mortality.Caution should be exercised in interpreting these findings given the low level of evidence of the studies. | Cai-Hong Li Dong-Kai Cheng Hong-Jun Yu Chun-Yi Li Hai-Qin Ren Ning Weng Bao-Shan Li Na Yang Peng Xu | 2020 | Reproductive and Developmental Medicine2020，4，3： | 0 |
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