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| 1 | Molecular diagnostics in a teacup:Non-Instrumented Nucleic Acid Amplification(NINA) for rapid,low cost detection of Salmonella enterica显示文摘We report on the use of a novel non-instrumented platform to enable a Loop Mediated isothermal Amplification(LAMP) based assay for Salmonella enterica.Heat energy is provided by addition of a small amount(<150 g) of boiling water,and the reaction temperature is regulated by storing latent energy at the melting temperature of a lipid-based engineered phase change material.Endpoint classification of the reaction is achieved without opening the reaction tube by observing the fluorescence of sequence-specific FRET-based assimilating probes with a simple handheld fluorometer.At or above 22℃ ambient temperature the non-instrumented devices could maintain reactions above a threshold temperature of 61℃ for over 90 min-significantly longer than the 60 min reaction time.Using the simple format,detection limits were less than 20 genome copies for reactions run at ambient temperatures ranging from 8 to 36℃.When used with a pre-enrichment step and non-instrumented DNA extraction device,trace contaminations of Salmonella in milk close to 1 CFU/mL could be reliably detected.These findings illustrate that the non-instrumented amplification approach is a simple,viable,low-cost alternative for field-based food and agricultural diagnostics or clinical applications in developing countries. | KUBOTA Ryo LABARRE Paul WEIGL Bernhard H LI Yong HAYDOCK Paul JENKINS Daniel M | 2013 | Chinese Science Bulletin2013，58，10： | 4 |
| 2 | Surface roughness of zirconia for full-contour crowns after clinically simulated grinding and polishing显示文摘The aim of this study was to evaluate the effect of controlled intraoral grinding and polishing on the roughness of full-contour zirconia compared to classical veneered zirconia. Thirty bar-shaped zirconia specimens were fabricated and divided into two groups(n515). Fifteen specimens(group 1) were glazed and 15 specimens(group 2) were veneered with feldspathic ceramic and then glazed. Prior to grinding,maximum roughness depth(Rmax) values were measured using a profilometer, 5 times per specimen. Simulated clinical grinding and polishing were performed on the specimens under water coolant for 15 s and 2 N pressure. For grinding, NTI diamonds burs with grain sizes of 20 mm, 10 mm, and 7.5 mm were used sequentially. The ground surfaces were polished using NTI kits with coarse, medium and fine polishers. After each step, Rmaxvalues were determined. Differences between groups were examined using one-way analysis of variance(ANOVA). The roughness of group 1 was significantly lower than that of group 2. The roughness increased significantly after coarse grinding in both groups. The results after glazing were similar to those obtained after fine grinding for non-veneered zirconia. However, fine-ground veneered zirconia had significantly higher roughness than venerred, glazed zirconia. No significant difference was found between fine-polished and glazed zirconia, but after the fine polishing of veneered zirconia, the roughness was significantly higher than after glazing.It can be concluded that for full-contour zirconia, fewer defects and lower roughness values resulted after grinding and polishing compared to veneered zirconia. After polishing zirconia, lower roughness values were achieved compared to glazing; more interesting was that the grinding of glazed zirconia using the NTI three-step system could deliver smooth surfaces comparable to untreated glazed zirconia surfaces. | Rim Hmaidouch Wolf-Dieter Mller Hans-Christoph Lauer Paul Weigl | 2014 | International Journal of Oral Science2014，6，4： | 2 |
| 3 | A new HPV-DNA test for cervical-cancer screening in developing regions: a cross-sectional study of clinical accuracy in rural China显示文摘 | You-lin Qiao John W Sellors Paul S Eder Yan-ping Bao Jeanette M Lim Fang-hui Zhao Bernhard Weigl Wen-hua Zhang Roger B Peck Ling Li Feng Chen Qing-jing Pan Attila T Lorincz | 2008 | Lancet Oncology2008，，10： | 1 |
| 4 | Detection of antigens in mycelial and in arthroconidial phases of Trichophyton mentagrophytes 显示文摘 | Weigl E Raska M | 1998 | Folia Microbiol (Praha)1998，43，6： | 1 |
| 5 | A novel missense mutation in theNDP gene in a child with Norrie disease and severe neurological in-volvement including infantile spasms 显示文摘 | Lev D Weigl Y Hasan M | 2007 | Am J Med Genet A2007，143，9： | 1 |
| 6 | Generation of s urvivin-specific CD8 ^+ T effector cells by dendritic cells pulsed with protein or selected peptides 显示文摘 | SCHMITZ M DIESTELKOETFER P WEIGLE B | 2000 | Cancer Res2000，60，17： | 1 |
| 7 | Antibody response to the tumor associated inhibitor of apoptosis protein survivin in cancer patients 显示文摘 | Rohaye m J Diestelkcetter P Weigle B | 2000 | Cancer Res2000，60，7： | 1 |
| 8 | Three-dimensional ultrasound of the pelvic floor 2 days after delivery:influence of conabitutional and obstetric factors显示文摘 | Falkert A Endress E Weigl M el al | 2010 | Ultrasound Obstet Gynecol2010，35，3： | 1 |
| 9 | Association between baseline plasma leptin levels and subsequent development of diabetes in Japanese Americans显示文摘 | McNeely M J Boyko E J Weigle D S | 1999 | Diabetes Care1999，22，1： | 1 |
| 10 | Work engagement accumulation of task, social, personal resources : A three-wave structural equation model显示文摘 | Weigl M Hornung S Parker S K | 2010 | Journal of Vocational Behavior2010，77，1： | 1 |
| 11 | Beyond top-down and bottom-up work redesign: Customizing job content through idiosyncratic deals显示文摘 | Homung S Rousseau D M Glaser J Angerer P and Weigl M | 2010 | Journal of Organizational Behavior2010，31，2： | 1 |
| 12 | Elevated free fatty acidsinduce uncoupling protein 3 expressionin muscle显示文摘 | WEIGLE D S SELFFRIDGE L E SCHW ARTZ M W | 1998 | Diabetes1998，47，： | 1 |
| 13 | Alkyl-substituted 2,6-dioxadiazolylpyridines as selective extractants for trivalent actinides 显示文摘 | WEIGL M MUELLICH U GEIST A | 2003 | J Radioanal Nuclear Chem2003，256，3： | 1 |
| 14 | Characteristics of radial growth and stable isotopes in a single oak tree to be used in climate studies显示文摘 | Weigl M Grabner M Helle G Schleser GH Wimmer R | | 0，，： | 1 |
| 15 | A systematic review of currently available pharmacological neuroprotective agents as s sole intervention before anticipated or induced cardiac arrest显示文摘 | Tenze G Steinlecchner B | 2005 | Resuscitation2005，65，： | 1 |
| 16 | Linking osteoarthritis-specific health-status measures to the international classification of functioning,disability,and health (ICF)显示文摘 | Weigl M Cieza A Harder M Geyh S Amann E Kostanjsek N | 2003 | Osteoarthritis Cartilage2003，11，： | 1 |
| 17 | Myometrial LH/hCG receptors during the estrous cycle and pregnancy in pigs显示文摘 | Rzucidlo S J Weigl R M Tihon J E | 1998 | Anim Reprod1998，51，： | 1 |
| 18 | Rotating stall control in a high speed stooge with inlel dis- tortion:Part H circumferential distortion显示文摘 | Spakovszky Z S Van Schalkwyk C M Weigl H J | 1999 | Journal of Turbomachinery1999，121，3： | 1 |
| 19 | Generation of survivin-specific CD8+T effector cells by dendritic cells pulsed with protein or selected peptides显示文摘 | Schmitz M Diestelkoetter P Weigle B | 2000 | Cancer Res2000，60，： | 1 |
| 20 | Generation of survivin-specific CD8+ T effector cells by dendritic cells pulsed with protein or selected peptides显示文摘 | Schmitz M Diestelkoetter P Weigle B | 2000 | Cancer Res2000，60，17： | 1 |
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