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| 1 | The ORF3a protein of SARS-CoV-2 induces apoptosis in cells显示文摘Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)has caused the ongoing pandemic of Coronavirus Disease 2019.SARS-CoV-2 belongs to the genus Betacoronavirus of the Coronaviridae family,which includes SARS-CoV and Middle East respiratory syndrome coronavirus.1,2 Coronavirus-encoded accessory proteins play critical roles in virus–host interactions and the modulation of host immune responses,thereby contributing to coronaviral pathogenicity via different strategies.3 However,the functions of SARS-CoV-2-encoded accessory proteins are not well understood.Apoptosis is a predominant type of programmed cell death,and has been recognized as an important host antiviral defense mechanism that controls viral infection and regulates the inflammatory response.4,5 Previous studies have reported that the SARS-CoV-encoded accessory protein ORF3a can induce apoptosis in cells,6,7 leading to the question of whether SARS-CoV-2 ORF3a also has pro-apoptotic activity.Here,we investigated the potential apoptosis-inducing activity of SARS-CoV-2 ORF3a in different cell lines and compared the pro-apoptotic activities of SARS-CoV-2 ORF3a with those of SARS-CoV ORF3a using the same system. | Yujie Ren Ting Shu Di Wu Jingfang Mu Chong Wang Muhan Huang Yang Han Xue-Yi Zhang Wei Zhou Yang Qiu Xi Zhou | 2020 | Cellular & Molecular Immunology2020，17，8： | 11 |
| 2 | Influence of CeO2 loading on structure and catalytic activity for NH3-SCR over TiO2-supported CeO2显示文摘A series of supported CeO2/TiO2 catalysts were prepared to explore the influence of CeO2 loading on these catalysts for the selective catalytic reduction of NO3 by NH3(NH3-SCR).The catalysts were investigated in detail by means of XRD,Raman,H2-TPR,NH3-TPD,XPS,in situ DRIFTS,and NH3-SCR reaction.The activity of the catalyst is closely related to the content of CeO2.When the loading of CeO2 is near the dispersion capacity(1.16 mmol Ce4+/100 m^2 TiO2),the catalytic activity is better.This may be because that the dispersed CeO2 is the active species and the catalyst has appropriate redox property,along with the larger amounts of surface Ce content and surface adsorbed oxygen species.Finally,a possible reaction mechanism via the Langmuir-Hinshelwood(L-H)mechanism is tentatively proposed to further understand the NH3-SCR reaction. | Hongliang Zhang Long Ding Hongming Long Jiaxin Li Wei Tan Jiawei Ji Jingfang Sun Changjin Tang Lin Dong | 2020 | Journal of Rare Earths2020，38，8： | 5 |
| 3 | Two unique C21-diterpenoid alkaloids from Aconitum carmichaelii显示文摘Two sulfonated diterpenoid alkaloids possessing different but related novel carbon skeletons,named aconidenusulfonine A(1)and 12,16-secoaconidenusulfonine A(2),respectively,were isolated as minor components from an aqueous extract of the lateral roots of Aconitum carmichaelii('Fu Zi').The structures of 1 and 2,representing the first two C21-diterpenoid alkaloids from nature,were determined by analysis of various spectroscopic data and chemical transformation,of which 1 was further proved by single-crystal X-ray diffraction.Especially,1 exhibited dose-depended analgesic activity consistent with the clinical function of Fu Zi. | Jingfang Zhang Xiaoqiang Lei Yazi Wei Hui Liu Qinglan Guo Tiantai Zhang Jiangong Shi | 2022 | Chinese Chemical Letters2022，33，12： | 4 |
| 4 | Docking and molecular dynamics studies on CYP2D6显示文摘Drug-metabolizing enzymes,also known as cytochrome P450s,are a superfamily of hemoglobin responsible for metabolizing more than 90% clinical drugs.Cytochrome P450 2D6(CYP2D6) is a significant member of cytochrome P450s for the reason of metabolizing about 20% clinical drugs.In this paper,molecular docking and molecular dynamic simulations are used to investi-gate the active site of CYP2D6,roles of essential amino acids within the active site and time-dependent protein energy changes.The results suggest that amino acids Glu216,Asp301,Ser304 and Ala305 in the active site are likely to form hydrogen bonding interactions with substrates;the benzene ring of Phe120 and aromatic ring in the substrates form Π-Π interactions.In addition,molecular dynamics simulations prove that the catalytic conformation of CYP2D6 without ligands can be obtained by their own atomic fluctuations.The impact of ligands on protein system energy and large conformational shift is not very large.Cytochrome P450s is known for their genetic polymorphisms,which will result in severe adverse drug reactions.Ideally,we hope to use mo-lecular modeling to investigate the differences between the substrates of wild-type and mutants while they are bonded with drugs,and predict the drug metabolizing ability of mutants.Reduce the possibility for people taking drugs that they can not metabolize,therefore reduce the rate of adverse drug reactions,and eventually establish a platform of personalized drugs to largely benefit human health. | WANG JingFang ZHANG ChengCheng WEI DongQing LI YiXue | 2010 | Chinese Science Bulletin2010，55，18： | 2 |
| 5 | Molecular dynamics simulations exploring drug resistance in HIV-1 proteases显示文摘Although HIV-1 subtype B still dominates the epidemic AIDS in developed countries,an increasing number of people in developing countries are suffering from an epidemic of non-subtype B viruses.What is worse,the efficacy of the combinational use of antiretroviral drugs is gradually compromised by the rapid development of drug resistance.To gain an insight into drug resistance, 10-ns MD simulations were simultaneously conducted on the complexes of the TL-3 inhibitor with 4 different proteases(Bwt,Bmut, Fwt and Fmut),among which the complex of the Bwt protease with the TL-3 inhibitor was treated as the control group.Detailed analyses of MD data indicated that the drug resistance of Bmut against TL-3 mainly derived from loss of an important hydrogen bond and that of Fwt was caused by the decrease of hydrophobic interactions in S1/S1'pocket,while both of the two reasons mentioned above were the cause of the Fmut protease's resistance.These results are in good agreement with the previous experiments, revealing a possible mechanism of drug resistance for the aforementioned protease subtypes against the TL-3 inhibitor.Additionally,another indication was obtained that the mutations of M36I,V82A and L90M may induce structural transforms so as to alter the inhibitor's binding mode. | GU Hui CHEN HaiFeng WEI DongQing WANG JingFang | 2010 | Chinese Science Bulletin2010，55，24： | 2 |
| 6 | Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp.B-3 on gene expression in RAW264.7 macrophage cells显示文摘B-3 exopolysaccharide is extracted from the Antarctic psychrophilic bacterium Psychrobacter sp. B-3. We have previously shown that it activates macrophages and affects their immunoregulatory activities. To determine what genes are affected during this process, we detected the genes differentially expressed in cells of RAW264.7 macrophages treated with B-3 exopolysaccharide by transcriptomic analysis. B-3 exopolysaccharide treatment caused differential expression of 420 genes, of which 178 were up-regulated and 242 were down-regulated. These genes were shown to be involved in many aspects of cell function, mainly metabolism and immunity. Genes were enriched in multiple immune-related pathways, and the most significantly enriched genes were involved in antigen processing and presentation pathways. The pathway in which differentially expressed genes were the most significantly enriched was the metabolic pathway; specifically, the expression of many metabolic enzyme genes was altered by B-3 exopolysaccharide treatment. Additionally, the genes involved in metabolisms of amino acids, carbohydrates, lipids and nucleotides, varied to certain degrees. B-3 exopolysaccharide, therefore, appears to directly affect the immune function of RAW264.7 macrophages as an immunostimulant, or to indirectly change intracellular metabolism. This is the first study to determine the effect of an Antarctic psychrophilic bacterial exopolysaccharide on RAW264.7 macrophages. Our findings provide an important reference for research into the regulation of macrophage immune function by different polysaccharides. | ZHANG Pingping LI Jiang YU Leiye WEI Jingfang XU Tong SUN Guojie | 2018 | Acta Oceanologica Sinica2018，37，8： | 1 |
| 7 | Theoretical study of the electronic ground states and low-lying singlet excited states of thiophene-based spirofluorenes显示文摘The electronic structures and optical properties of oligothiophene substituted spirofluorenes are investigated theoretically with semi-empirical quantum chemical calculations. A theoretical investigation of the interaction between two perpendicular π-systems of various oligothiophene substituted spirofluorenes is conducted. The results demonstrate that the interaction between two perpendicular branches is reduced by oligothiophene substitutions. Photoexcitation induced relaxation is mainly located on one of the equivalent branches or the branch with longer conjugation length. In addition to these benefits brought by 9,9-spirobifluorene center, the specific oligothiophene moieties linked to the spiro center also have properties of significant merit, such as the ability to tune energy levels and emission colors by controlling the conjugation length. | CHEN RunFeng MA Cong PAN JingFang ZHENG Chao HUANG Wei | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，5： | 1 |
| 8 | Theoretical investigation of substitution and end-group effects on poly(p-phenylene vinylene)s显示文摘Semi-empirical AM1 and ZINDO/S,as well as density function theory(DFT)method B3LYP/6-31G(d)quantum chemical calculations were carried out to study the electronic structures and optical properties of poly(p-phenylene vinylene)derivatives(PPVs)with 10 and 11 phenylene rings in the backbone.The calculations suggest that the assembly of alternate incorporation of CN and alkoxy substituted phenylene rings in the PPV backbone could be a good way to construct organic semiconductors with low HOMO/LUMO energy band-gaps.The effect of the end-group on the electronic structures and optical properties of the conjugated polymer was investigated by the calculated UV-Vis and UPS spectra.It was demonstrated that the aldehyde and phosphate end-groups have limited effects on the photophysical properties in the UV-Visible range. | ZHENG Chao PAN JingFang CHEN RunFeng WANG Yang HUANG Wei | 2014 | Science China Chemistry2014，57，3： | 0 |
| 9 | The structure of phospholamban and its MD simulations显示文摘Phospholamban is an important protein with responsibility for regulating the activity of the sarcoplasmic reticulum Ca2+ pump through reversible phosphorylation.And its three-dimensional structure in living cell has been a focus of attention.In the current case, we summarized the investigations on phospholamban structure, and on this base, employed long time-scale molecular dy-namics simulations to study its structure systematically.The first 22 residues from one chain of phospholamban in bellflower structure determined by NMR experiments, together with its phosphorylation at position 16 and mutation at position 9 were picked up as three different systems.By molecular dynamics simulations of 10 ns in the explicit solution surroundings, it was found that the 3–15 residues of the original structure retained their helix structures, while the phosphorylation and mutation had less probability to form helix structures.These structural changes might result in inhibition decrease to the sarcoplasmic reticulum Ca2+ pump, which is in accordance with previous experimental results. | TANG Bei GONG Ke WANG JingFang LI YiXue WEI DongQing | 2010 | Chinese Science Bulletin2010，55，16： | 0 |
| 10 | Universality class of machine learning for critical phenomena显示文摘Herein,percolation phase transitions on a two-dimensional lattice were studied using machine learning techniques.Results reveal that different phase transitions belonging to the same universality class can be identified using the same neural networks(NNs),whereas phase transitions of different universality classes require different NNs.Based on this finding,we proposed the universality class of machine learning for critical phenomena.Furthermore,we investigated and discussed the NNs of different universality classes.Our research contributes to machine learning by relating the NNs with the universality class. | Gaoke Hu Yu Sun Teng Liu Yongwen Zhang Maoxin Liu Jingfang Fan Wei Chen Xiaosong Chen | 2023 | Science China(Physics,Mechanics & Astronomy)2023，66，12： | 0 |
| 11 | Transgressive Events since the Late Pleistocene in the Yellow River Delta: Grain-size Distribution and Palynological Results显示文摘This study deals with the relationship between sea-level changes and paleoclimatic fluctuations based on the analysis of stratigraphy, grain sizes, palynology, and radiometric dating of the Yellow River delta since the Late Pleistocene. Evidence from the sedimentary record, grain sizes, and pollen provides a paleoenvironmental history of the Late Pleistocene from the boreholes of the delta. Based on a combination of grain-size analysis with lithological studies, marine deposit units contain the intervals of 13.85–16.9, 18.5–19.69, 27.9–34.8, 36.4–37.2, 48.4–51.6, and 54.1–55.9 m, and transitional facies units contain the intervals of 10.25–13.85, 16.9–18.5, 19.69–27.9, 34.8–36.4, 37.2–48.4, 51.6–54.1, and 55.9–60 m, compared with fluvial(terrestrial facies) deposit units(3.36–10.25 m). Based on pollen analysis and pollen assemblages, there were three warm-wet periods from 9.1–0.16 ka BP, 16.1–60 ka BP, and 90.1–94.6 ka BP From the top to the bottom of the borehole, the paleoclimate has an evident fluctuation: warm and moist(Holocene Optimum) —cool and dry(Younger Dryas Event)—mild semi cool—cool and dry—warm and moist. There were three warm-wet periods from 9.1–0.16 ka BP, 16.1–60 ka BP, and 90.1–94.6 ka BP, corresponding to the Holocene Optimum stage, MIS 3, and MIS 5, respectively. The warm period allowed monsoonal evergreen and broadleaved deciduous forests that corresponded to Holocene hypsithermal climatic conditions and the Late Pleistocene climatic Optimum. Three warm-wet periods occurred in marine deposit units from 9.1–0.16 ka BP, 60.1–16.1 ka BP, and 94.6–90.1 ka BP. These periods correspond to the Cangzhou transgression, Xianxian transgression, and Huanghua transgression, respectively. From 90.1–60.1 ka BP, 17.5–9.1 ka BP, and 0. 16 ka BP–1855 AD, three dry and cold phases are recognized. The phases indicate the fluvial(flood plain) sedimentary environment, corresponding to cooler and mild dry periods based on palynological results and grain-size distribution. | LU Jingfang LIU Jian HUANG Wei HU Gang ZHANG Daolai John BIRKS | 2020 | Acta Geologica Sinica(English Edition)2020，94，4： | 0 |
| 12 | Heterogenous glucose-stimulated insulin secretion at single islet level显示文摘Insulin secretion by pancreatic islets plays a vital role in regulating blood glucose levels.Nevertheless,the mech-anism responsible for this dynamic insulin secretion has not been completely understood,particularly at the single islet level.In this study,we have successfully developed an easy microfluidic platform that allows for the exploration of dynamic glucose-stimulated insulin secretion(GSIS)at the single islet level.With the utilization of this platform,we evaluated dynamic GSIS from single islets isolated from both normal and diabetic rats.Our results demonstrate that islets can be categorized into three types based on their dynamic GSIS:Type Ⅰ exhibits a biphasic GSIS profile with a fast first phase and flat second phase;Type Ⅱ also has a biphasic GSIS profile with a fast first phase but a slow increased second phase;Type Ⅲ displays only a slowly increased second phase and lacks a fast first phase.RNA sequencing analysis demonstrated that the cell type and exocytosis-specific genes are consistent with the proportion of cells and insulin release kinetics among the three types of islets,respectively.Moreover,our findings suggest that high expression of Atp5pb is anti-correlated with the first phase of insulin secretion.Furthermore,we revealed that diabetic islets exhibit only the type Ⅰ GSIS response,indicating a deliberate impairment of the second phase of insulin secretion.Together,this device serves as a crucial tool in the research field of islets and diabetes,allowing researchers to investigate islet functional heterogeneity and identity at the single islet level. | Jiaxiang Yin Hao Meng Haopeng Lin Meijun Mo Jingfang Lin Jingyi Chen Lihua Chen Xiaojun Xu Zonghong Li Wei Ji Tao Xu Huisheng Liu | 2023 | Engineered Regeneration2023，4，4： | 0 |
| 13 | Solid-phase impregnation promotes Ce doping in TiO_(2) for boosted denitration of CeO_(2)/TiO_(2) catalysts显示文摘CeO_(2)/TiO_(2)(denoted as Ce Ti) catalysts obtained by solid-phase impregnation behaved better in lowtemperature selective catalytic reduction of NO_(x)with NH_(3)(NH_(3)-SCR) than that by conventional wet impregnation.To explore the main factors for activity distinction,the texture property,CeO_(2)dispersion and structure changes of TiO_(2)were comprehensively analyzed.It was found that surface changes of TiO_(2)had a significant impact on the improved activity.From results of inductively coupled plasma atomic emission spectrometer (ICP-AES),diffuse reflectance UV-vis spectroscopy (UV-vis-DRS) and Raman,it was inferred that Ce ions were partially incorporated into TiO_(2)lattice,accompanied with the formation of defects and vacancies during solid-phase impregnation.Accordingly,Ce Ti catalysts from solid-phase impregnation exhibited superiority in adsorption and activation of reactants.Further result from monitoring the preparation process indicated that the evolved NO played an important role in promoting Ce doping through depriving oxygen atoms on TiO_(2)surface.The interaction between Ce and Ti was enhanced.The catalyst performed better in NH_(3)-SCR,especially at low temperature,which testified the solid-phase impregnation could be an effective method to modulate interface structure for designing efficient catalyst. | Wang Song Jiawei Ji Kai Guo Xin Wang Xiaoqian Wei Yandi Cai Wei Tan Lulu Li Jingfang Sun Changjin Tang Lin Dong | 2022 | Chinese Chemical Letters2022，33，2： | 0 |
| 14 | Specific ion effects on soil aggregate stability and rainfall splash erosion显示文摘Soil interparticle forces can pose important effects on soil aggregate stability and rainfall splash erosion.Meanwhile,these interparticle forces are strongly influenced by specific ion effects.In this study,we applied three monovalent cations(Li^(+),Na^(+),and K^(+))with various concentrations to investigate the influence of specific ion effects on aggregate stability and splash erosion via pipette and rainfall simulation methods.The specific ion effects on soil interparticle forces were quantitatively evaluated by introducing cationic non-classical polarization.The results showed that aggregate stability and splash erosion had strong ion specificity.Aggregate breaking strength and splash erosion rate at the same salt concentration followed the sequence as Li^(+)>Na^(+)>K^(+).With decreasing salt concentration,the difference in aggregate breaking strength or splash erosion rate between different cation systems increased initially(1–10^(-2)mol L^(–1))and later was nearly invariable(10^(–2)–10^(–4)mol L^(–1)).The experimental results were well quantitatively explained by soil interparticle forces considering cationic non-classical polarization.Furthermore,both aggregate breaking strength and splash erosion rate of three cations revealed a strong positive linear relation with net force subjected to cationic non-classical polarization(R^(2)=0.81,R^(2)=0.81).These results demonstrated that different non-classical polarization of cations resulted in different soil interparticle forces,and thus led to differences in aggregate stability and splash erosion.Our study provides valuable information to deeply understand the mechanisms of rainfall splash erosion. | Jingfang Liu Feinan Hu Chenyang Xu Wei Du Zhenghong Yu Shiwei Zhao Fenli Zheng | 2022 | International Soil and Water Conservation Research2022，10，4： | 0 |
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