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| 1 | Lack of correlation between Treg quantification assays in inflammatory bowel disease patients显示文摘AIM:To compare the number of regulatory T-cells( Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR(q PCR) method in patients suffering from inflammatory bowel disease(IBD).METHODS:Tregs percentages obtained by both flow cytometry and q PCR methods in 35 adult IBD patients,18 out of them with Crohn′s disease(CD)and 17 with ulcerative colitis(UC)were compared to each other as well as to scores on two IBD activity questionnaires using the Harvey Bradshaw Index(HBI)for CD patients and the Simple Colitis Clinical Activity Index(SCCAI)for UC patients.The Treg percentages by flow cytometry were defined as CD4+CD25highCD127lowFOXP3+cells in peripheral blood mononuclear cells,whereas the Treg percentages by q PCR method were determined as FOXP3 promoter demethylation in genomic DNA.RESULTS:We found an average of 1.56%±0.78%Tregs by using flow cytometry,compared to 1.07%±0.53%Tregs by using q PCR in adult IBD patients.There were no significant correlations between either the percentages of Tregs measured by flow cytometry or q PCR and the HBI or SCCAI questionnaire scores in CD or UC patients,respectively.In addition,there was no correlation between Treg percentages measured by q PCR and those measured by flow cytometry(r=-0.06,P=0.73;Spearman Rho).These data suggest that,either Treg-related immune function or the clinical scores in these IBD patients did not accurately reflect actual disease activity.Until the cause(s)for these differences are more clearly defined,the resultssuggest caution in interpreting studies of Tregs in various inflammatory disorders.CONCLUSION:The two methods did not produce equivalent measures of the percentage of total Tregs in the IBD patients studied which is consistent with the conclusion that Tregs subtypes are not equally detected by these two assays. | Gunnar Brandhorst Darinka Todorova Petrova Sebastian Weigand Christoph Eberle Nicolas von Ahsen Jessica Schmitz Frank Christian Schultze Dirk Raddatz Michael Karaus Michael Oellerich Philip D Walson | 2015 | World Journal of Gastroenterology2015，21，11： | 2 |
| 2 | Primary adenocarcinoma of the urinary bladder ,a clinicopathological and prognostic study 显示文摘 | Anderstrom C Joganssonn SL Von Schultz | 1983 | Cancer1983，52，： | 1 |
| 3 | Primary adenocarcinoma of the urinary bladder,a clinico-pathological and prognostic study显示文摘 | Anderstrom C Jogansson SL Von Schultz | 1983 | Cancer1983，52，： | 1 |
| 4 | CD40 activation:potential for specific immunotherapy in B-CLL显示文摘 | von Bergwelt-Baildon M Maecker B Schultze J | 2004 | Ann Oncol2004，15，6： | 1 |
| 5 | Primary adenocarcinoma of the urinary bladder: a clinicopathologic and prognostic study显示文摘 | Anderstrom C Johansson S L von Schultz L | 1983 | Cancer1983，52，： | 1 |
| 6 | Giant negative magnetoresistance in perovskitelike La(2/3)Ba(1/3) MnOx ferromagnetic films显示文摘 | Von Helmolt R Wecker J Holzapfel B Schultz L Samwer K | 1993 | Physical Review Letters1993，71，： | 1 |
| 7 | The moderating role of internal and external resources on the performance effect of multitasking:Evidence from the R&D performance of surgeons显示文摘 | Schultz C Schreyoegg J von Reitzenstein C | 2013 | Research Policy2013，42，13： | 1 |
| 8 | Giant magnetoresistance in melt spun Cu Co alloys 显示文摘 | WECKER J VON HELMOLT R SCHULTZ L | 1993 | Applied Physics Letters1993，62，16： | 1 |
| 9 | 查看详情显示文摘 | von Helmolt R Wecker J Holzapfel B Schultz L Samwer K | | 0，，： | 1 |
| 10 | DCs and CD40-activated B cells:current and future avenues to cellular cancer immunotherapy显示文摘 | Schultze J L Grabbe S von Berqwelt-Baildon M S | 2004 | Trends Immunol2004，25，12： | 1 |
| 11 | Primary adenocarcinoma of the urinary bladder, a clinicopathological and prognostic study 显示文摘 | Anderstrom C Jogansson SL Von Schultz | 1983 | Cancer1983，52，： | 1 |
| 12 | Giant negative magnetoresistance in perovskite like La2/3Ba1/3MnOx ferromagnetic films 显示文摘 | Helmolt R von Holzapfel B Schultz L | 1993 | Phys Rev Lett1993，71，： | 1 |
| 13 | Enhancement of N-formamideinduced carcinogenesis by urinary tract infection in rats显示文摘 | Johansson S L Anderstrom C Von Schultz L | 1987 | Cancer Res1987，47，2： | 1 |
| 14 | FoxP3 demethylation is increased in human colorectal cancer and rat cholangiocarcinoma tissue显示文摘 | Frank Christian Schultze Reiner Andag Salamah Mohammad Alwahsh Draga Toncheva Svilen Maslyankov Nikolay Yaramov Nicolas von Ahsen Gunnar Brandhorst Philip D. Walson Michael Oellerich Darinka Todorova Petrova | 2013 | Clinical Biochemistry2013，，： | 1 |
| 15 | T cell responses to hepatitis B surface antigen are detectable in non-vaccinated individuals显示文摘AIM: To evaluate, whether humoral hepatitis-B-vaccine non-responders also fail to mount a T cell response and to compare these results to normal vaccinees. METHODS: Fourty-seven health care employees were enrolled in this study including all available non- responders (n = 13) with an anti-HBsAg titer < 10 kU/L and all available low-responders (n = 12) with an anti- HBsAg titer < 100 kU/L. Also, 12 consecutive anti-HBsAg negative pre-vaccination subjects were enrolled as well as 10 subjects (+7 from the vaccinated group) with titers > 1000 kU/L as controls. PBMC from all subjects were analyzed by IFN-γ and IL-4 ELISPOT assays for the presence of hepatitis B surface antigen (HBsAg) reactive T cells. RESULTS: Non-responders and low-responders had no or only very limited T cell responses, respectively. Indi- viduals responding to vaccination with the induction of a high anti-HBsAg titer showed a strong T cell response after the third vaccination. Surprisingly, these individuals showed response even before the first vaccination. T cell response to control antigens and mitogens was similar in all groups. CONCLUSION: Our data suggest that there is no gen- eral immune deficiency in non-/low-responders. Thus, we hypothesize that the induction of anti-HBsAg re- sponses by vaccination is significantly dependent on the pre-existing T cell repertoire against the specific antigen rather than the presence of a general T cell defect. | Martin R Weihrauch Michael von Bergwelt-Baildon Milos Kandic Martin Weskott Winfried Klamp Joachim Rsler Joachim L Schultze | 2008 | World Journal of Gastroenterology2008，14，16： | 1 |
| 16 | Primary adonocarcinoma of the urinary bladder,a clinicopathological and prognostic study显示文摘 | NDERSTROM C JOGANSSON S L VON SCHULTZ L | 1983 | Cancer1983，52，4： | 1 |
| 17 | Diabetic keratopathy显示文摘 | Schultz RO Von Hom Peter MA | 1981 | Trans Am Ophthalmol Soc1981，79，2： | 1 |
| 18 | Primary adenocarcinoma of the urinary bladder:a clinicopathologic and prognostic study显示文摘 | Anderstrm C Johansson SL von Schultz L | 1983 | Cancer1983，52，7： | 1 |
| 19 | Primary adenocarinoma of the urinary bladder:A clinicopathologic and prognostic study显示文摘 | Anderst'm C Johansson SL von Schultz L | | 0，，： | 1 |
| 20 | Giant magnetoresistance in melt spun Cu-Co alloys显示文摘 | Wecker J von Helmolt V Schultz L | | 0，，16： | 1 |
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