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| 1 | Silybin counteracts lipid excess and oxidative stress in cultured steatotic hepatic cells显示文摘AIM: To investigate in vitro the therapeutic effect and mechanisms of silybin in a cellular model of hepatic steatosis.METHODS: Rat hepatoma Fa O cells were loaded with lipids by exposure to 0.75 mmol/L oleate/palmitate for 3 h to mimic liver steatosis. Then, the steatotic cells were incubated for 24 h with different concentrations(25 to 100 μmol/L) of silybin as phytosome complex with vitamin E. The effects of silybin on lipid accumulation and metabolism, and on indices of oxidative stress were evaluated by absorption and fluorescence microscopy, quantitative real-time PCR, Western blot, spectrophotometric and fluorimetric assays.RESULTS: Lipid-loading resulted in intracellular triglyceride(TG) accumulation inside lipid droplets, whose number and size increased. TG accumulation was mediated by increased levels of peroxisome proliferator-activated receptors(PPARs) and sterol regulatory element-binding protein-1c(SREBP-1c). The lipid imbalance was associated with higher production of reactive oxygen species(ROS) resulting in increased lipid peroxidation, stimulation of catalase activity and activation of nuclear factor kappa-B(NF-κB). Incubation of steatotic cells with silybin 50 μmol/L significantly reduced TG accumulation likely by promoting lipid catabolism and by inhibiting lipogenic pathways, as suggested by the changes in carnitine palmitoyltransferase 1(CPT-1), PPAR and SREBP-1c levels. The reduction in fat accumulation exerted by silybin in the steatotic cells was associated with the improvement of the oxidative imbalance caused by lipid excess as demonstrated by the reduction in ROS content, lipid peroxidation, catalase activity and NF-κB activation.CONCLUSION: We demonstrated the direct antisteatotic and anti-oxidant effects of silybin in steatotic cells, thus elucidating at a cellular level the encouraging results demonstrated in clinical and animal studies. | Giulia Vecchione Elena Grasselli Adriana Voci Francesca Baldini Ignazio Grattagliano David QH Wang Piero Portincasa Laura Vergani | 2016 | World Journal of Gastroenterology2016，22，26： | 13 |
| 2 | Decortication for Chronic Parapneumonic Empyema: Results of a Prospective Study显示文摘 | Giulio Melloni Angelo Carretta Paola Ciriaco Giampiero Negri Carlopietro Voci Giuseppe Augello Piero Zannini | 2004 | World Journal of Surgery2004，，5： | 2 |
| 3 | Direct effects of iodothyronines on excess fat storage in rat hepatocytes显示文摘 | Elena Grasselli Adriana Voci Laura Canesi Rita De Matteis Fernando Goglia Federica Cioffi Emilia Fugassa Gabriella Gallo Laura Vergani | 2010 | Journal of Hepatology2010，，6： | 2 |
| 4 | Value of echocardiography in predicting future cardiac events after acute myocardial infarction显示文摘 | Voci P Romeo F | 2003 | Curr Opin Cardiol2003，18，5： | 1 |
| 5 | Intergroup contact and prejudice toward immigrants in Italy: The mediational role of anxiety and the moderational role of group salience 显示文摘 | Voci A Hewstone M | 2003 | Group Processes Intergroup Relations2003，6，1： | 1 |
| 6 | Successful weaning from cardiopulmonary bypass with central venous prostaglandin E and left atrial norepinephrine infusion in patients with acute pulmonary hypertention 显示文摘 | Tritapepe L Voci P Cogliati AA | 1999 | Crit Care Med1999，27，10： | 1 |
| 7 | Nonivasive coronary flow reserve assessed by transthoracic coronary Doppler ultrasound in patients- with left anterior descending coronary artery stents显示文摘 | Pizzuto F Voci P Mariano E | 2003 | Am J Cardi- ol2003，91，5： | 1 |
| 8 | Uncoupling protein 2 mRNA exp -ression and respiratory parameters in kupffer cells isolated from euthyroid and hyperthyroid rat livers 显示文摘 | Demori I Franzi AT | 2001 | Eur J Endocrionol2001，145，3： | 1 |
| 9 | Assessment of flow velocity reserve by transthoracic Doppler echocardiography and venous adenosine infusion before and after left anterior descending coronary artery stenting显示文摘 | Pizzuto F Voci P Mariano E | 2001 | J Am Coll Cardiol2001，38，4： | 1 |
| 10 | Noninvasive coronary flow reserve assessed by transthoracic coronary doppler ultrasound in patients with left anterior descending coronary artery stents显示文摘 | Francesco Pizzuto Paolo Voci Enrica Mariano | 2003 | Am J Cardiol2003，91，： | 1 |
| 11 | Imaging of the distal left anterior descending coronary artery by transthoracic color Doppler echocardiography显示文摘 | Testa G Plaustro G | 1998 | The America Journal of Cardiology1998，18，12： | 1 |
| 12 | Uncoupling protein 2 mRNA expression and respiratory parameters in Kupffer cells isolated from euthyroid and hyperthyroid rat livers 显示文摘 | Voci A Demori I Franzi AT | 2001 | Eur J Endocrinol2001，145，3： | 1 |
| 13 | Estimating the gradient threshold in the Pemna- Malic equation显示文摘 | VOCI F EIHO S SUGIMUID N | 2004 | IEEE Signal Processing Magazine2004，3946，： | 1 |
| 14 | Tissue-type plasminogen activator therapy Versus primary coronary angioplasty:impact on myocardial tissue pefusion and regional function显示文摘 | Agati L Voci P Hickle P | 1998 | J Am Cell Cardial1998，31，： | 1 |
| 15 | Direct effects of Bisphenol A on lipid homeostasis in rat hepatoma cells显示文摘 | Grasselli E Cortese K Voci A | 2013 | Chemosphere2013，91，8： | 1 |
| 16 | Bortezomib and gemcitabine in relapsed or refractory Hodgkin's lymphoma显示文摘 | Mendler JH Kelly J Voci S | 2008 | Ann Oncol2008，19，10： | 1 |
| 17 | Coronary recanalization in anterior myocardial infarction: the open perforator hypothesis显示文摘 | VOCI P MARIANO E PIZZUTO F | 2002 | J Am Coll Cardiol2002，40，： | 1 |
| 18 | Estimating the gradient threshold in the Perona-Malik equation 显示文摘 | Voci F Eiho S SugJmoto N | 2004 | IEEE Signal Pro- cessing Magazine2004，21，3： | 1 |
| 19 | Noninvasive surrogate marrers of atherosclerosis显示文摘 | Voci P Pizzuto F | 2002 | Am J Cardiol2002，89，： | 1 |
| 20 | Assessment of Myocardial Reperfusion by Intravenous Myocardial Contrast Echocardiography and Coronary Flow Reserve After Primary Percutaneous Transluminal Coronary Angiography in Patients With Acute Myocardial Infarction显示文摘 | Wolfgang Lepper Rainer Hoffmann Otto Kamp Andreas Franke Carel C. de Cock Harald P. Kühl Gertjan T. Sieswerda Jürgen vom Dahl Uwe Janssens Paolo Voci Cees A. Visser Peter Hanrath | 2000 | Circulation: Journal of the American Heart Association2000，，20： | 1 |
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