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您的检索式：作者名="Vincent Bulone"
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| 1 | What Do We Really Know about Cellulose Biosynthesis in Higher Plants?显示文摘Cellulose biosynthesis is one of the most important biochemical processes in plant biology. Despite the considerable progress made during the last decade, numerous fundamental questions related to this key process in plant development are outstanding. Numerous models have been proposed through the years to explain the detailed molecular events of cellulose biosynthesis. Almost all models integrate solid experimental data with hypotheses on several of the steps involved in the process. Speculative models are most useful to stimulate further research investigations and bring new exciting ideas to the field. However, it is important to keep their hypothetical nature in mind and be aware of the risk that some undemonstrated hypotheses may progressively become admitted. In this review, we discuss the different steps required for cellulose formation and crystallization, and highlight the most important specific aspects that are supported by solid experimental data. | Gea Guerriero Johanna Fugelstad Vincent Bulone | 2010 | Journal of Integrative Plant Biology2010，52，2： | 14 |
| 2 | Biosynthesis of Callose and Cellulose by Detergent Extracts of Tobacco Cell Membranes and Quantification of the Polymers Synthesized in vitro显示文摘The conditions that favor the in vitro synthesis of cellulose from tobacco BY-2 cell extracts were determined. The procedure leading to the highest yield of cellulose consisted of incubating digitonin extracts of membranes from 11-day-old tobacco BY-2 cells in the presence of 1 mM UDP-glucose, 8 mM Ca2+ and 8 mM Mg2+. Under these conditions, up to nearly 40% of the polysaccharides synthesized in vitro corresponded to cellulose, the other polymer synthesized being callose. Transmission electron microscopy analysis revealed the occurrence of two types of structures in the synthetic reactions. The first type consisted of small aggregates with a diameter between 3 and 5 nm that associated to form fibrillar strings of a maximum length of 400 nm. These structures were sensitive to the acetic/nitric acid treatment of Updegraff and corresponded to callose. The second type of structures was resistant to the Updegraff reagent and corresponded to straight cellulose microfibrils of 2-3 nm in diameter and 200 nm to up to 5 μm in length. In vitro reactions performed on electron microscopy grids indicated that the minimal rate of microfibril elongation in vitro is 120 nm/min. Measurements of retardance by liquid crystal polarization microscopy as a function of time showed that small groups of microfibrils increased in retardance by up to 0.047 nm/min per pixel, confirming the formation of organized structures. | Carolina Cifuentes Vincent Bulone Anne Mie C.Emons | 2010 | Journal of Integrative Plant Biology2010，52，2： | 2 |
| 3 | Identification and Preliminary Characterization of a New Chemical Affecting Glucosyltransferase Activities Involved in Plant Cell Wall Biosynthesis显示文摘化学遗传作为化学 genomics 的部分是一条强大、快的发展中的途径把因为基因冗余性或致命性,可能对困难的生物过程描绘使用常规遗传并且在多糖生合成的情况中,种灵活性。多糖合成的酶位于二台主要 compartmentsthe Golgi 仪器,血浆 membraneand 能用膜部分在 vitro 被学习。这里,我们首先开发了允许在 glycosyltransferase 活动与潜在的效果屏蔽化学药品的一个图书馆的高产量的试金。从在 Golgi glucosyltransferases 上为他们的效果屏蔽的 4800 化学药品,从主要屏幕的 66 混合物在糖类生合成上有效果。十这些混合物被证实在第二幅屏幕以后禁止葡萄糖加入。展出强壮的抑制效果(标志 6240780 命名化学 A ) 的一混合物被选择并且进一步学习了。它 reversibly 由 Golgi 膜从 UDP 葡萄糖禁止葡萄糖的转移,但是激活血浆膜界限 callose synthase。抑制效果依赖于混合物的化学结构,它不影响植物房间的 endomembrane 形态学,但是在房间墙作文引起变化。化学 A 为 Golgi 和血浆膜界限 glucosyltransferases 的机制的学习与一个大潜力代表新奇的药。 | Olga Zabotina Erik Maim Georgia Drakakaki Vincent Bulone Natasha Raikhel | 2008 | Molecular Plant2008，1，6： | 1 |
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