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| 1 | Increased catabolism and decreased unsaturation of ganglioside in patients with inflammatory bowel disease显示文摘AIM: To investigate whether accelerated catabolism of ganglioside and decreased ganglioside content contribute to the etiology of pro-inflammatory intestinal disease. METHODS: Intestinal mucosa from terminal ileum or colon was obtained from patients with ulcerative colitis or inflammatory Crohn's disease(n = 11) undergoing bowel resection and compared to control samples of normal intestine from patients with benign colon polyps(n = 6) and colorectal cancer(n = 12) in this observational case-control study. Gangliosides and phospholipids of intestinal mucosa were characterized by class and ceramide or fatty acid composition using liquid chromatography triple-quad mass spectrometry. Content and composition of ganglioside classes GM1, GM3, GD3, GD1 a, GT1 and GT3 were compared among subject groups. Content and composition of phospholipid classes phosphatidylcholine(PC) and phosphatidylethanolamine were compared among subject groups. Unsaturation index of individual ganglioside and phospholipid classes was computed and compared among subject groups. Ganglioside catabolism enzymes beta-hexosaminidase A(HEXA) and sialidase-3(NEU3) were measured in intestinal mucosa using western blot and compared among subject groups. RESULTS: Relative GM3 ganglioside content was 2-fold higher(P < 0.05) in intestine from patients with inflammatory bowel disease(IBD) compared to control intestine. The quantity of GM3 and ratio of GM3/GD3 was also higher in IBD intestine than control tissue(P < 0.05). Control intestine exhibited 3-fold higher(P < 0.01) relative GD1 a ganglioside content than IBD intestine. GD3 and GD1 a species of ganglioside containing three unsaturated bonds were present in control intestine, but were not detected in IBD intestine. The relative content of PC containing more than two unsaturated bonds was 30% lower in IBD intestine than control intestine(P < 0.05). The relative content of HEXA in IBD intestine was increased 1.7-fold(P < 0.05) and NEU3 was increased 8.3-fold(P < 0.01) compared to normal intestine. Intestinal mucosa in IBD is characterized by increased GM3 content, decreased GD1 a, and a reduction in polyunsaturated fatty acid constituents in GD3, GD1 a and PC.CONCLUSION: This study suggests a new paradigm by proposing that IBD occurs as a consequence of increased metabolism of specific gangliosides. | John J Miklavcic Glen K Shoemaker Vera C Mazurak M Tom Clandinin Tasha DL Hart Kareena L Schnabl Gordon M Lees Bodil MK Larsen Oliver F Bathe Alan BR Thomson M Tom Clandinin | 2015 | World Journal of Gastroenterology2015，21，35： | 3 |
| 2 | bla (CTX-M-2)and bla(CTX-M-28)e xtendedspectrum beta-lactamase genes and class 1 integrons in clinical isolates of Klebsiella pneumoniae from Brazil显示文摘 | Lopes AC Veras DL Lima AM | 2010 | Mem Inst Os waldo Cruz2010，105，2： | 1 |
| 3 | Isolation of independent gene pairs at two loci for seed coat color in Brassica juncea 显示文摘 | Vera CL Woods DL | 1982 | Can J Plant Sci1982，62，： | 1 |
| 4 | Bla(cTX_M_2) and bla( CTX-M- 28) extendedspectrumbeta-lactamase genes and class l integrons in clinical isolates of KlebsieUa pneumoniae from Brazil显示文摘 | Lopes AC Veras DL Lima AM | 2010 | Mem Inst Os waldo Cruz2010，105，2： | 1 |
| 5 | UltrastructuralChanges in Clinical and Microbiota Isolates of Klebsiellapneumoniae Carriers of Genes bla SHV, bla TEM, blaCTX-M, or bla KPC When Subject to ^-Lactam Antibi-otics 显示文摘 | Veras DL Lopes AC da Silva GV | 2015 | Scientific World Journal2015，15，6： | 1 |
| 6 | Ultrastructural changes in clinical and microbiota isolates of Klebsiella pneumoniae carriers of genes bla SHV,bla TEM,bla CTX-M,or bla KPC when subject toβ-lactam antibiotics显示文摘 | Veras DL Lopes AC da Silva GV | 2015 | Sci World J2015，2015，57： | 1 |
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