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| 1 | Modern diagnostic approaches to cholangiocarcinoma显示文摘BACKGROUND:Cholangiocarcinoma is a very aggressive tumor with poor survival.Therefore,early diagnosis and surgical resection are of paramount importance.Its diagnosis is difficult because access to the tumor is not easy.Biopsy is possible only for intrahepatic cholangiocarcinoma,which accounts for 10% of cases.Routine brush cytology from endoscopic retrograde cholangiopancreatography(ERCP) has a high specificity of 100% but unfortunately a low sensitivity of 30%.In this review we briefly describe new diagnostic techniques applicable to ERCP brush cytology specimens and targeting the genetic background of the disease,in particular fluorescence in situ hybridization(FISH) and digital image analysis(DIA).DATE SOURCES:The PubMed database up to 2011 was used for the retrieval of relevant articles.The search terms FISH,fluorescence in situ hybridization,DIA,digital image analysis and cholangiocarcinoma were used.Both original and review articles were used.RESULTS:FISH identifies cells with chromosomal abnormalities,mainly numerical aberrations,using a mixture of fluorescencelabeled probes.FISH offers a higher sensitivity than routine cytology,retaining a high level of specificity.The DIA criterion for malignancy is demonstration of aneuploidy.This technique increases the sensitivity to 40%,but the specificity remains low.Preliminary data from application to other tumors suggest that combination of FISH and DIA may be of further benefit.CONCLUSIONS:The new techniques offer a significantly enhanced diagnostic efficacy in the evaluation of ERCP brush specimens.Apart from contributing to a more timely diagnosis,their wider application to cholangiocarcinoma may also facilitate the genetic study of the disease and add to our understanding of oncogenesis at the molecular level,with the prospect of identifying targets for novel therapeutic interventions. | Larisa E Vasilieva Stefanos I Papadhimitriou Spyros P Dourakis | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，4： | 2 |
| 2 | The antiplatelet effect of atorvastatin in patients with acute coronary syndrome depends on the hs-CRP level显示文摘 | Vasilieva E Kasyanova O Shpektor A | 2008 | Acute Card Care2008，10，3： | 1 |
| 3 | Brachial artery flow-mediated dilation in pa-tients with Tako-Tsubo cardiomyopathy显示文摘 | VASILIEVA E VOROBYEVA I LEBEDEVA A | 2011 | Am J Med2011，124，： | 1 |
| 4 | The antiplatelet effect of atorvastatin in patients with acute coronary syndrome depends on the hs-CRP level 显示文摘 | VASILIEVA E KASYANOVA O SHPEKTOR A | 2008 | Acute Card Care2008，10，： | 1 |
| 5 | Struc-tural and tectonic peculiarities of structure and prospects ofoil and gas potential of the northern part of Barents-Kara re-gion显示文摘 | Dolgunov K A Martirosyan V N Vasilieva E A | 2011 | Oil and Gas Geology2011，，6： | 1 |
| 6 | The antiplatelet effect of ator vastatin in Patients with acute coronary syndrome depend sonthehs-CRP level显示文摘 | Vasilieva E Kasyanova O Shpektor A | 2008 | Acute Card Care2008，10，3： | 1 |
| 7 | The antiplatelet effect of atorvastatin inpatientswith acute coronary syndrome depends on the hs - CRP level 显示文摘 | Vasilieva E Kasyanova O ShpektorA | 2008 | AcuteCard Care2008，10，3： | 1 |
| 8 | BACE2, a β-secretase homolog, leaves at the β site and within the amyloid-β region of the amyloid-β precursor protein 显示文摘 | FARZAN M SCHNITZLER C E VASILIEVA N | 2000 | Proc Natl Acad Sci USA2000，97，17： | 1 |
| 9 | The antiplatelet effect of atorvastatin in patients with acute coronary syndrome depends on the hs-CRP level显示文摘 | Vasilieva E Kasyanova O Shpektor A | 2008 | Acute Card Care2008，10，3： | 1 |
| 10 | , A tyrosine-sulfated peptide based on the N terminus of CCR5 interacts with a CD4 enhanced epitope of the HIV-1 gp120 envelope glycoprotein and inhibits HIV-1 entry显示文摘 | Farzan M Vasilieva N Sehnitzler C E | 2000 | J Biol Chem2000，275，33： | 1 |
| 11 | Odors providing sexual information in Djungarian hamsters:evidence for an across-odor code显示文摘 | LAI S C VASILIEVA N Y JOHNSTON R E | 1996 | Horm Behav1996，3，： | 1 |
| 12 | The antiplate|et effect of atorvastatin in patients with acute coronary syndrome depends on the hs-CRP level显示文摘 | Vasilieva E KasyanovaO ShpektorA | 2008 | Acute Card Care2008，10，3： | 1 |
| 13 | Mapping small DNA ligand hydroxyl radical footprinting and affinity cleavage products for capillary electrophoresis 显示文摘 | He G Vasilieva E Bashkin J K | 2013 | Analytical Biochemistry2013，439，： | 1 |
| 14 | The antiplatelet effect of atorvastatin in patients with acute coronary syndrome depends on the hs-CRP level显示文摘 | Vasilieva E Kasyanova O Shpektor A | 2008 | Acute Card Care2008，10，3： | 1 |
| 15 | The antiplatelet effect of atorvastatin in patients with acute coronary syndrome depends on the hs-CRP level显示文摘 | Vasilieva E Kasyanova O Shpektor A | 2008 | Acute Card Care2008，10，3： | 1 |
| 16 | A tyrosine-sulfated peptide based on the N terminus of CCR5 interacts with a CD4-enhanced epitope of the HIV-1 gp120 envelope glycoprotein and inhibits HIV-1entry显示文摘 | VASILIEVA N CHRISTINE E | 2000 | J Biol Chem2000，275，33： | 1 |
| 17 | The antiplatelet effect of atorvastatin in patients with acute coronary syndrome depends on the hs-CRP level显示文摘 | Vasilieva E Kasyanova O Shpektor A | 2008 | Acute Card Care2008，10，3： | 1 |
| 18 | The antiplatelet effect of ator- vastatin in patients with acute coronary syndrome depends on the hs- CRP level显示文摘 | Vasilieva E Kasyanova O Shpektor A | 2008 | Acute Card Care2008，10，3： | 1 |
| 19 | BACE2,a beta- secretase homolog, cleaves at the beta site and within the amy loid--beta region of the amyloid-beta precursor protein显示文摘 | Farzan M Schnitzler C E Vasilieva N | 2000 | Proc Natl Acad Sci USA2000，97，： | 1 |
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