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| 1 | Expression of von Hippel-Lindau tumor suppressor and tumor-associated carbonic anhydrases IX and XII in normal and neoplastic colorectal mucosa显示文摘AIM: To analyze possible relationships between CA ⅠⅩ/CA Ⅻ and pVHL expression in normal and neoplastic colorectal mucosa.METHODS: Immunohistochemical staining of 42 tissue specimens obtained from 17 cancer patients was performed to evaluate the distribution and semi-quantitatively assess the levels of CA ⅠⅩ, CA Ⅻ and pVHL. VHL mRNAs from 14 fresh-frozen tumors was amplified by RT-PCR and subjected to sequencing. CA9 and CA12 mRNA levels were analyzed by semi-quantitative RT-PCR in comparison with VEGFas an indicator of hypoxia that uncouples the pVHL control.RESULTS: Tumor tissues were associated with a borderline increase of CA ⅠⅩ staining signal and slight but significant decrease of CA Ⅻ immunoreactivity, whereas no association was found for pVHL. Sequence analysis of RT-PCR-amplified VHL mRNAs revealed no deletions/mutations, suggesting that they were VHL-competent. We did not observe any correlation between pVHL and CA ⅠⅩ/CA Ⅻ proteins as well as between VEGF and CA9 mRNAs, but the tumor-associated changes in mRNA levels of VEGF and CA12 showed a significant inverse relationship.CONCLUSION: Our results indicate that CA9 and CA12 are regulated by different intratumoral factors and that lack of apparent relationship between the levels of CA ⅠⅩ/CA Ⅻ and pVHL cannot be fully assigned to uncoupling of negative regulatory function of pVHL by tumor hypoxia signified by induced VEGF transcription. The interplay between the functional pVHL and CA ⅠⅩ/CA Ⅻ in colorectal tumors seems rather complex and is not evident merely at the expression levels. | Antti J. Kivela Seppo Parkkila Juha Saarnio Tuomo J. Karttunen Jyrki Kivela Anna-Kaisa Parkkila Maria Bartosova Vojtech Mucha Michal Novak Abdul Waheed William S. Sly Hannu Rajaniemi Silvia Pastorekova Jaromir Pastorek | 2005 | World Journal of Gastroenterology2005，11，17： | 10 |
| 2 | Divergent expression of bacterial wall sensing toll-like receptors 2 and 4 in colorectal cancer显示文摘AIM To characterize the expression of toll-like receptors(TLR) 2 and 4 in colorectal cancer(CRC) and in normal colorectal mucosa.METHODS We analysed tissue samples from a prospective series of 118 unselected surgically treated patients with CRC. Sections from formalin fixed, paraffin embedded specimens were analysed for TLR2 and TLR4 expression by immunohistochemistry. Two independent assessors evaluated separately expression at the normal mucosa, at the invasive front and the bulk of the carcinoma, and in the lymph node metastases when present. Expression levels in different locations were compared and their associations with clinicopathological features including TNM-stage and the grade of the tumour and 5-year follow-up observations were analysed.RESULTS Normal colorectal epithelium showed a gradient of expression of both TLR2 and TLR4 with low levels in the crypt bases and high levels in the surface. In CRC, expression of both TLRs was present in all cases and in the major proportion of tumour cells. Compared to normal epithelium, TLR4 expression was significantly weaker but TLR2 expression stronger in carcinoma cells. Weak TLR4 expression in the invasive front was associated with distant metastases and worse cancerspecific survival at 5 years. In tumours of the proximal colon the cancer-specific survival at 5 years was 36.9% better with strong TLR4 expression as compared with those with weak expression(P = 0.044). In contrast, TLR2 expression levels were not associated with prognosis. Tumour cells in the lymph node metastases showed higher TLR4 expression and lower TLR2 expression than cells in primary tumours.CONCLUSION Tumour cells in CRC show downregulation of TLR4 and upregulation of TLR2. Low expression of TLR4 in the invasive front predicts poor prognosis and metastatic disease. | Karoliina Paarnio Sara Vayrynen Kai Klintrup Pasi Ohtonen Markus J Makinen Jyrki Makela Tuomo J Karttunen | 2017 | World Journal of Gastroenterology2017，23，26： | 8 |
| 3 | Overexpression of HSP27 and HSP70 is associated with decreased survival among patients with esophageal adenocarcinoma显示文摘BACKGROUND Overexpression of heat shock proteins(HSPs) is associated with several malignancies and contributes to the development, progression, and metastasis of cancer, in addition to the inhibition of cellular death. In recent years, there has been active research into using HSP inhibitors in several malignancies. Due to the poor prognosis of esophageal adenocarcinoma(EAC), it would be valuable to find new biomarkers for the development of cancer treatments.AIM To evaluate the expressions of HSP27 and HSP70 and their effect on survival in EAC.METHODS Immunohistochemical analyses and evaluations of HSP27 and HSP70 expression were performed on all available samples from 93 patients diagnosed with EACbetween 1990 and 2007 at two university hospitals. Fifteen cases with Barrett's metaplasia and 5 control cases from the same patient population were included in the analysis. HSP expression was quantitatively assessed and classified as high or low. Kaplan-Meier analyses and Cox regression models adjusting for age and sex as well as tumor site, stage, and grade were used to evaluate the effect on survival.RESULTS Tumor stage and surgical treatment were the main prognostic factors. High HSP27 expression in cancer cases was a strong negative predictive factor, with a mean survival of 23 mo compared to the 49 mo in cases with a low expression(P= 0.018). The results were similar for HSP70, with a poorer survival of 17 mo in cases with high HSP70 expression, in contrast to 40 mo(P = 0.006) in cases with a low expression. A Cox regression survival analysis was performed, adjusting for possible confounding factors, and higher HSP27 and HSP70 expressions remained an independent negative prognostic factor. The HSPs' correlation with survival was not affected by cancer treatments. When the analysis was adjusted for all factors, the odds ratios for HSP27 and HSP70 were 3.3(CI: 1.6–6.6, P =0.001) and 2.2(CI: 1.2–3.9, P = 0.02), respectively.CONCLUSION HSP27 and HSP70 overexpression is associated with poor survival in EAC, which is, to the best of our knowledge, reported for the first time. | Henna K S?derstr?m Juha T Kauppi Niku Oksala Timo Paavonen Leena Krogerus Jari R?s?nen Tuomo Rantanen | 2019 | World Journal of Clinical Cases2019，7，3： | 7 |
| 4 | Epithelial cell proliferation and glandular atrophy in lymphocytic gastritis: Effect of H pylori treatment显示文摘AIM: Lymphocytic gastritis is commonly ass ociated with Helicobacter pylori infection. The presence of glandular atrophy and foveolar hyperplasia in lymphocytic gastritis suggests abnormalities in cell proliferation and differentiation,forming a potential link with the suspected association with gastric cancer. Our aim was to compare epithelial cell proliferation and morphology in H pylori associated lymphocytic gastritis and H pylori gastritis without features of lymphocytic gastritis,and to evaluate the effect of H pylori treatment.METHODS: We studied 14 lymphocytic gastritis patients with H pylori infection. For controls, we selected 14 matched dyspeptic patients participating in another treatment trial whose H pylori infection had successfully been eradicated.Both groups were treated with a triple therapy and followed up with biopsies for 6-18 months (patients) or 3 months (controls). Blinded evaluation for histopathological features was carried out. To determine the cell proliferation index,the sections were labeled with Ki-67 antibody.RESULTS: Before treatment, lymphocytic gastritis was characterized by foveolar hyperplasia (P=0.001) and glandular atrophy in the body (P=0.008), and increased proliferation in both the body (P=0.001) and antrum (P=0.002). Proliferation correlated with foveolar hyperplasia and inflammation activity. After eradication therapy, the number of intraepithelial lymphocytes decreased in the body (P=0.004)and antrum (P=0.065), remaining higher than in controls (P<0.001). Simultaneously, the proliferation index decreased in the body from 0.38 to 0.15 (P=0.043), and in the antrum from 0.34 to 0.20 (P=0.069), the antral index still being higher in lymphocytic gastritis than in controls (P=0.010).Foveolar hyperplasia and glandular atrophy in the body improved (P=0.021), reaching the non-LG level.CONCLUSION: In lymphocytic gastritis, excessive epithelial cell proliferation is predominantly present in the body, where it associates with foveolar hyperplasia and glandular atrophy.These characteristic changes of lymphocytic gastritis are largely related to H pylori infection, as shown by their improvement after eradication. However, some residual deviation was still seen in lymphocytic gastritis, indicating either an abnormally slow improvement or the presence of some persistent abnormality. | Johanna M.Mkinen Seppo Niemel Tuomo Kerola Juhani Lehtola Tuomo J.Karttunen | 2003 | World Journal of Gastroenterology2003，9，12： | 2 |
| 5 | Incorporating futures research into regional knowledge creation and management显示文摘 | TUOMO UOTILA HELINA MELKAS VESA HARMAAKORPI | 2005 | Futures2005，，37： | 1 |
| 6 | Circulating pregnancy as sociated plasma protein a predicts outcome in patients with acute coronary syndromes but no troponin I elevation显示文摘 | Juha M Qiu-Ping Q Tuomo I | 2003 | Circulation2003，108，6： | 1 |
| 7 | Temperature optimisation of a diesel engine using exhaust gas heat recovery and thermal energy storage (diesel engine with thermal energy storage)显示文摘 | Pertti Kauranen Tuomo Elonen Lisa Wikstr?m Jorma Heikkinen Juhani Laurikko | 2009 | Applied Thermal Engineering2009，，6： | 1 |
| 8 | A Method for Assessing Absorptive Capacity of a Re-gional Innovation System显示文摘 | HARMAAKORPI V MELKAS H | 2006 | FENNIA2006，184，1： | 1 |
| 9 | Promoting network-based organizational innovations: A new approach in Finnish labour and technology policies 显示文摘 | ALASOINI Tuomo | 2001 | International Journal of Technology Management2001，22，13： | 1 |
| 10 | Immunohistochemical Study of Colorectal Tumors for Expression of a Novel Transmembrane Carbonic Anhydrase, MN/CA IX, with Potential Value as a Marker of Cell Proliferation显示文摘 | Juha Saarnio Seppo Parkkila Anna-Kaisa Parkkila Kari Haukipuro Silvia Pastoreková Jaromir Pastorek Matti I. Kairaluoma Tuomo J. Karttunen | 1998 | The American Journal of Pathology1998，，1： | 1 |
| 11 | Size distributions of mass and chemical components in street-level and rooftop PM1 particles in Helsinki 显示文摘 | Tuomo A Pakkanena Veli-Matti Kerminena Kati Loukkolaa | 2003 | Atmospheric Environment2003，37，： | 1 |
| 12 | Early markers of myocardial injury: cTnI is enough显示文摘 | Tuomo I Juha L Pekka P | 2009 | Clin Chim Acta2009，400，12： | 1 |
| 13 | Effect of regular exercise on homocysteine concentrations: the HERITAGE Family Study显示文摘 | Tomohiro Okura Tuomo Rankinen Jacques Gagnon Suzanne Lussier-Cacan Jean Davignon Arthur S. Leon D. C. Rao James S. Skinner Jack H. Wilmore Claude Bouchard | 2006 | European Journal of Applied Physiology2006，，： | 1 |
| 14 | Enzyme activ-ities of rumen particles and feed samples incubated in situ with diffe-ring types of cloth显示文摘 | Pekka Huhtanen Aila Yanhatalo Tuomo Varvikko | 1998 | Bridsh Journal of Nutrition1998，79，： | 1 |
| 15 | No association between the angiotensin-converting enzyme ID polymorphism and elite endurance athlete status显示文摘 | RANKINEN TUOMO BERND WOLFARTH JEAN-AIME SIMONEAU | 2000 | J Appl Physiol2000，88，： | 1 |
| 16 | Towards defining the neuropathological substrates of vascular dementia显示文摘 | Raj N. Kalaria Rose Anne Kenny Clive G. Ballard Robert Perry Paul Ince Tuomo Polvikoski | 2004 | Journal of the Neurological Sciences2004，，： | 1 |
| 17 | A cluster approach for the adsorption of oxygen and carbon monioxide on SnO2 and CdS surfaces显示文摘 | Tuomo S Golovanov V Lantto V | 1995 | Sensors and Actuators B1995，25，1： | 1 |
| 18 | A conceptual framework for identifying the need and role of models in the implementation of the Water Framework Directive显示文摘 | Tuomo M Saloranta | 2003 | Intl J River Basin Management2003，1，4： | 1 |
| 19 | Circulating pregnancy-associated plasma protein A predicts outcome in patients with acute coronary syndromes but no troponin I elevation显示文摘 | Juha M Qiu-ping Q Tuomo I | 2003 | Circulation2003，108，： | 1 |
| 20 | Human leucocyte antigen and TNFα polymorphism association in microscopic colitis显示文摘 | Ritva M. Koskela Tuomo J. Karttunen Seppo E. Niemel? Juhani K. Lehtola Jorma Ilonen Riitta A. Karttunen | 2008 | European Journal of Gastroenterology & Hepatology2008，，4： | 1 |
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