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| 1 | Antisense MMP-9 RNA inhibits malignant glioma cell growth in vitro and in vivo显示文摘The matrix-degrading metalloproteinases (MMPs), particularly MMP-9, play important roles in the pathogenesis and development of malignant gliomas. In the present study, the oncogenic role of MMP-9 in malignant glioma cells was investigated via antisense RNA blockade in vitro and in vivo. TJ905 malignant glioma cells were transfected with pcDNA3.0 vector expressing antisense MMP-9 RNA (pcDNA-AS-MMP9), which significantly decreased MMP-9 expression, and cell proliferation was assessed. For in vivo studies, U251 cells, a human malignant glioma cell line, were implanted subcutaneously into 4-to 6-week-old BALB/c nude mice. The mice bearing well-established U251 gliomas were treated with intratumoral pcDNA-AS-MMP9-Lipofectamine complex (AS-MMP-9-treated group), subcutaneous injection of endostatin (endostatin-treated group), or both (combined therapy group). Mice treated with pcDNA (empty vector)-Lipofectamine served as the control group. Four or eight weeks later, the volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity were assayed. We demonstrate that pcDNA-AS-MMP9 significantly decreased MMP-9 expression and inhibited glioma cell proliferation. Volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity in the antisense-MMP-9-treated and therapeutic alliance groups were significantly lower than those in the control group. The results suggest that MMP-9 not only promotes malignant glioma cell invasiveness, but also affects tumor cell proliferation. Blocking the expression of MMP-9 with antisense RNA substantially suppresses the malignant phenotype of glioma cells, and thus can be used as an effective therapeutic strategy for malignant gliomas. | Cuiyun Sun Qian Wang Hongxu Zhou Shizhu Yu Alain R. Simard Chunsheng Kang Yanyan Li Yanling Kong Tongling An Yanjun Wen Fudong Shi Junwei Hao | 2013 | Neuroscience Bulletin2013，29，1： | 14 |
| 2 | Data fusion using Bayesian theory and reinforcement learning method显示文摘Dear editor,In recent years,the theory and method of mission planning technology have been widely applied to bio-robotic systems.Increasing demands can be addressed by the application of innovative and advanced technology in mission planning and decision-making of bio-robotic systems. | Tongle ZHOU Mou CHEN Chenguang YANG Zhiqiang NIE | 2020 | Science China(Information Sciences)2020，63，7： | 5 |
| 3 | Host Interferon-Stimulated Gene 20 Inhibits Pseudorabies Virus Proliferation显示文摘Host interferon-stimulated gene 20(ISG20)exerts antiviral effects on viruses by degrading viral RNA or by enhancing IFN signaling.Here,we examined the role of ISG20 during pseudorabies virus(PRV)proliferation.We found that ISG20 modulates PRV replication by enhancing IFN signaling.Further,ISG20 expression was upregulated following PRV infection and poly(I:C)treatment.Ectopic expression of ISG20 inhibited PRV proliferation in PK15 cells,whereas knockdown of ISG20 promoted PRV proliferation.In addition,ISG20 expression upregulated IFN-βexpression and enhanced IFN downstream signaling during PRV infection.Notably,PRV UL24 suppressed the transcription of ISG20,thus antagonizing its antiviral effect.Further domain mapping analysis showed that the N terminus(amino acids 1-90)of UL24 was responsible for the inhibition of ISG20 transcription.Collectively,these findings characterize the role of ISG20 in suppressing PRV replication and increase the understanding of host-PRV interplay. | Xiaoyong Chen Dage Sun Sujie Dong Huanjie Zhai Ning Kong Hao Zheng Wu Tong Guoxin Li Tongling Shan Guangzhi Tong | 2021 | Virologica Sinica2021，36，5： | 4 |
| 4 | A Classification–Detection Approach of COVID-19 Based on Chest X-ray and CT by Using Keras Pre-Trained Deep Learning Models显示文摘The Coronavirus Disease 2019(COVID-19)is wreaking havoc around the world,bring out that the enormous pressure on national health and medical staff systems.One of the most effective and critical steps in the fight against COVID-19,is to examine the patient’s lungs based on the Chest X-ray and CT generated by radiation imaging.In this paper,five keras-related deep learning models:ResNet50,InceptionResNetV2,Xception,transfer learning and pre-trained VGGNet16 is applied to formulate an classification-detection approaches of COVID-19.Two benchmark methods SVM(Support Vector Machine),CNN(Conventional Neural Networks)are provided to compare with the classification-detection approaches based on the performance indicators,i.e.,precision,recall,F1 scores,confusion matrix,classification accuracy and three types of AUC(Area Under Curve).The highest classification accuracy derived by classification-detection based on 5857 Chest X-rays and 767 Chest CTs are respectively 84%and 75%,which shows that the keras-related deep learning approaches facilitate accurate and effective COVID-19-assisted detection. | Xing Deng Haijian Shao Liang Shi Xia Wang Tongling Xie | 2020 | Computer Modeling in Engineering & Sciences2020，，11： | 4 |
| 5 | Reinforcement Learning Based Data Fusion Method for Multi-Sensors显示文摘In order to improve detection system robustness and reliability, multi-sensors fusion is used in modern air combat. In this paper, a data fusion method based on reinforcement learning is developed for multi-sensors. Initially, the cubic B-spline interpolation is used to solve time alignment problems of multisource data. Then, the reinforcement learning based data fusion(RLBDF) method is proposed to obtain the fusion results. With the case that the priori knowledge of target is obtained, the fusion accuracy reinforcement is realized by the error between fused value and actual value. Furthermore, the Fisher information is instead used as the reward if the priori knowledge is unable to be obtained. Simulations results verify that the developed method is feasible and effective for the multi-sensors data fusion in air combat. | Tongle Zhou Mou Chen Jie Zou | 2020 | IEEE/CAA Journal of Automatica Sinica2020，7，6： | 3 |
| 6 | Non-equilibrium alloy phase formation and transformation driven by ion beam mixing in the Fe-Hf-Nb multilayers显示文摘In the ion beam mixing experiments,eight Fe-Hf-Nb multilayered films,with overall compositions of Fe67Hf22Nb11,Fe67Hf11Nb22,Fe54Hf38Nb8,Fe54Hf30Nb16,Fe54Hf11Nb35,Fe25Hf67Nb8,Fe25Hf50Nb25 and Fe25Hf11Nb64,were irradiated by 200 keV xenon ions to doses ranging from 3×1014 Xe+/cm2 to 7×1015 Xe+/cm2.The results showed that unique amorphous phases were obtained at designed alloy compositions,falling in the favored glass-forming region deduced from three binary metal sub-systems.Interestingly,at some alloy compositions,the crystal-amorphous-crystal transformations were observed back and forth while varying the irradiation doses.In addition,at the alloy composition of Fe25Hf67Nb8,a metastable FCC phase was formed through an HCP-FCC structural phase transformation and it had a large lattice constant identified to be a=4.51 .Besides,the formation mechanism of non-equilibrium alloy phases was also discussed in terms of thermodynamics of solids and atomic collision theory. | LI Nan WANG TongLe DING Ning DAI Ye BAI Xue LIU BaiXin | 2012 | Science China(Technological Sciences)2012，55，3： | 2 |
| 7 | Comparison of Viromes in Ticks from Different Domestic Animals in China显示文摘Ticks are involved in the transmission of various arboviruses and some tick-borne viruses pose significant threats to the health of humans or livestock.This study aimed to investigate the geographical distribution of tick species and tickassociated viruses in central and eastern China.Total 573 ticks from domestic animals including dogs,sheep and cattle were collected in 2017.Two genera of ticks were identified including Rhipicephalus and Haemaphysalis.Sequencing was performed on Miseq Illumina platform to characterize the tick viromes from the four different sampling locations.Following trimming,13,640 reads were obtained and annotated to 19 virus families.From these sequences,above 37.74%of the viral reads were related to the RNA viruses.Virome comparison study revealed that the tick viral diversity was considerably different in the two identified tick genera.The viral diversity of R.microplus was significantly different from that of other Rhipicephalus species.On the other hand,substantial overlap in viral species was observed between the same genera.In addition,we found no evidence that the natural host played a major role in shaping virus diversity based on the comparison of their viromes.Rather,the geographic location seems to significantly influence the viral families.Phylogenetic study indicated that the novel negative-sense RNA viruses identified in this study was closely related to Bole tick virus 1 and 3 viruses.In conclusion,the present study provides a baseline for comparing viruses detected in ticks,according to species,natural hosts,and geographic locations. | Tingting Zhao Haiyan Gong Xiaojuan Shen Wen Zhang Tongling Shan Xiangqian Yu Seong Jin Wang Li Cui | 2020 | Virologica Sinica2020，35，4： | 2 |
| 8 | Dynamic monitoring of changes in endothelial cell-substrate adhesiveness during leukocyte adhesion by microelectrical impedance assay显示文摘到在发炎过程的 endothelial 房间的白血球的粘附导致 endothelial 房间底层粘着性的变化,并且这的理解变化将向我们提供发炎过程的重要信息。在这研究,我们使用了叫作在 endothelial 房间底层粘着性监视变化的电子传感器(RT-CES ) 系统以一种动态、量的方式由人的 monoblastic 房间线 U937 房间粘附导致了的即时房间的一个非侵略的生物传感器系统。这试金,基于房间底层阻抗读出,能由于 U937 房间粘附在房间底层粘着性监视短暂变化。对 endothelial 房间的 U937 房间粘附被 lipopolysaccharide (LPS ) 以一种剂量依赖者方式导致。尽管到 endothelial 房间的支持者 U937 房间的数字被标准试金验证,在 U937 房间的增加以后的 endothelial 房间的粘着性被 RT-CES 系统监视。而且,在 endothelial 房间的焦点的粘附 kinase 蛋白质减少和 F 肌动朊重新整理在 U937 房间的增加以后被观察。我们的结果显示到对待 LPS 的 endothelial 房间的 U937 房间的粘附把房间粘着性归结为底层,和如此的减小可能便于白血球的渗入。 | Yakun Ge Tongle Deng Xiaoxiang Zheng | 2009 | Acta Biochimica et Biophysica Sinica2009，41，3： | 2 |
| 9 | CHOICE OF FILLING MATERIALS AND TECHNIQUES IN XINQIAO PYRITE MINE显示文摘CHOICEOFFILLINGMATERIALSANDTECHNIQUESINXINQIAOPYRITEMINE①ZhangQinli,WangXinminColegeofResource,EnvironmentandCivilEnginering,... | Zhang Qinli, Wang Xinmin College of Resource, Environment and Civil Engineering, Central South University of Technology, Changsha 410083 Chen Jiasheng Xinqiao Pyrite Mine, Tongling 244131 | 1997 | 中国有色金属学会会刊:英文版1997，7，4： | 2 |
| 10 | Metallic glass formation in the ternary Ni-Nb-Mo system by ion beam mixing显示文摘Glass forming ability of the ternary Ni-Nb-Mo system was studied by ion beam mixing of the Ni-Nb-Mo multilayered films.In the experiment,metallic glasses i.e.amorphous alloys were formed in the Ni51Nb19Mo30,Ni52Nb35Mo13,Ni61Nb15Mo24 and Ni72Nb20Mo8 multilayered films,while only solid solutions were obtained in the Ni24Nb29Mo47 and Ni26Nb53Mo21 multilayered films.It turned out that the Ni concentration played a dominating role in affecting the glass-forming ability of the system.Besides,thermodynamic calculations predicted a favored composition region for metallic glass formation,matching well with the observations from ion beam mixing. | LI Yang WANG TongLe DING Ning LIU JianBo LIU BaiXin | 2012 | Science China(Technological Sciences)2012，55，8： | 2 |
| 11 | Bex2 regulates cell proliferation and apoptosis in malignant glioma cells via the c-Jun NH2-terminal kinase pathway显示文摘 | Xiuping Zhou Qingming Meng Xuebin Xu Tongle Zhi Qiong Shi Yong Wang Rutong Yu | 2012 | Biochemical and Biophysical Research Communications2012，，3： | 1 |
| 12 | An intense 14 MeV neutron source显示文摘 | SU Tongling SUN Biehe YANG Baotai | 1990 | Nuclear Instruments and Methods in Physics Research A1990，287，45： | 1 |
| 13 | Design status of an intense 14 MeV neutron source for cancer therapy显示文摘 | YAO Zeen SU Tongling CHEN Shangwen | 2002 | Nuclear Instruments and Methods in Physics Research A2002，490，3： | 1 |
| 14 | The relationship between adverse reac- tions and composition of Shuanghuanglian injection[J]显示文摘 | TongL | 1997 | 中成药1997，19，4： | 1 |
| 15 | ClaynetworkinABSGgraftGMAHnanocomposites:rheologyandflammability显示文摘 | MaH TongL XuZ etal | 2007 | PolyGmerDegradationandStability2007，92，8： | 1 |
| 16 | Screening for plant extracts to control potato late blight显示文摘 | WANG Shutong HU Tongle ZHANG Fengqiao | 2007 | Frontiers of Agriculture in China2007，1，1： | 1 |
| 17 | Genomic Characterization and High Prevalence of Bovaviruses in Swine 显示文摘 | Shan Tongling Lan Daoliang Li Linlin | 2011 | PLoS One2011，6，17： | 1 |
| 18 | An intense 14 MeV neutron source显示文摘 | SU Tongling SUN Biehe YANG Baotai | 1990 | Nucl Instrum Methods1990，287，： | 1 |
| 19 | Design status of an intense 14 MeV neutron source for cancer therapy显示文摘 | YAO Zeen SU Tongling CHENG Shangwen | 2002 | Nucl Instrum Methods2002，490，： | 1 |
| 20 | Fusing Carbocycles of Inequivalent Ring Size to a Bis(imino)pyridine-Iron Ethylene Polymerization Catalyst: Distinctive Effects on Activity, PE Molecular Weight, and Dispersity显示文摘The 4,6-bis(arylimino)-1,2,3,7,8,9,10-heptahydrocyclohepta[b]quinoline-iron(II)chlorides(aryl=2,6-Me_(2)C_(6)H_(3) Fe1;2,6-Et_(2)C_(6)H_(3) Fe_(2);2,6-i-Pr_(2)C_(6)H_(3) Fe_(3);2,4,6-Me3C_(6)H_(2) Fe4;and 2,6-Et_(2)-4-Me_(2)C_(6)H_(2) Fe_(5))have been prepared in good yield by a straightforward one-pot reaction of 2,3,7,8,9,10-hexahydro-1H-cyclohepta[b]quinoline-4,6-dione,FeCl_(2)·4H_(2)O,and the appropriate aniline in acetic acid.All ferrous complexes have been characterized by elemental analysis and FT-IR spectroscopy.In addition,the structure of Fe_(3) has been determined by single crystal X-ray diffraction,which showed the iron center to adopt a distorted square pyramidal geometry with the saturated sections of the fused six-and sevenmembered carbocycles to be cis-configured.In combination with either MAO or MMAO,Fe1–Fe5 exhibited exceptionally high activities for ethylene polymerization(up to 15:86×10^(6) g(PE)mol^(−1)(Fe)h^(−1) at 40℃(MMAO)and 9:60×10^(6) g(PE)mol^(−1)(Fe)h^(−1) at 60℃(MAO))and produced highly linear polyethylene(HLPE,Tm≥128℃)with a wide range in molecular weights;in general,the MMAO-promoted polymerizations were more active.Irrespective of the cocatalyst employed,the 2,6-Me_(2)-substituted Fe1 and Fe4 proved the most active while the more sterically hindered 2,6-i-Pr_(2) Fe_(3) the least but afforded the highest molecular weight polyethylene(Mw:65.6–72.6 kg mol^(-1)).Multinuclear NMR spectroscopic analysis of the polymer formed using Fe4/MMAO at 40℃ showed a preference for fully saturated chain ends with a broad bimodal distribution a feature of the GPC trace(Mw/Mn=13:4).By contrast,using Fe4/MAO at 60℃ a vinyl-terminated polymer of lower molecular weight(Mw=14:2 kg mol^(−1))was identified that exhibited a unimodal distribution(Mw/Mn=3:8).Moreover,the amount of aluminoxane cocatalyst employed,temperature,and run time were also found to be influential on the modality of the polymer. | Zheng Wang Gregory A.Solan Yanping Ma Qingbin Liu Tongling Liang Wen-Hua Sun | 2019 | Research2019，，1： | 1 |
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