| 4 | Effects of muscle fiber orientation to main magnetic field on muscle metabolite profiles for magnetic resonance spectroscopy acquisition显示文摘BACKGROUND Proton magnetic resonance spectroscopy(~1H MRS) is a technique widely used for investigating metabolites in humans. Lipids are stored outside the muscle cell are called extramyocellular lipids(EMCL), and lipids stored on the inside of muscle cells are called intramyocellular lipids(IMCL). The relationship between metabolic syndrome and IMCL has been extensively studied.AIM To determine the effects of muscle fiber orientations on muscle metabolites using ~1H MRS.METHODS Chicken muscles were used as the subject in this study. MRS spectra were performed on a 1.5 T Magnetic resonance imaging machine(1.5 Tesla Philips Achieva). A single voxel(8 mm × 8 mm × 20 mm) was placed on the chicken extensor iliotibialis lateralis muscle with the muscle fiber oriented at 0°, 30°, 60°,and 90° to the main magnetic field. ~1H MRS spectra were acquired using a pointresolved spectroscopy, TR = 2000 ms, TE = 30 ms, and NSA = 256. Metabolites of interest from each orientation to the main magnetic field were compared using Wilcoxon signed-rank test. Differences less than 0.05 were considered to be statistically significant with 95%CI.RESULTS The metabolite profiles were different for each orientation of muscle fibers to the main magnetic field. The orientation at 90° was the most different compared to other orientations. The quantity of IMCL and EMCL exhibited statistically significantly changes with impacts at 30°, 60°, and 90° when compared with muscles aligned at 0° to the main magnetic field. Statistical analysis showed statistically significant IMCL(CH_3), EMCL(CH_3), and IMCL(CH_2) at 30°, 60°, and 90°(P = 0.017, 0.018, and 0.018, respectively) and EMCL(CH_2) at 30° and 60°(P =0.017 and 0.042, respectively). EMCL(CH_2) at 90° was unable to be measured in this study. The muscle lipids quantified at 30°, 60°, and 90° tended to be lower when compared to 0°.CONCLUSION Careful positioning is one of the most important factors to consider when studying ~1H MRS metabolites in muscles to ensure reproducibility and uniformity of muscle metabolite spectra. | Duanghathai Pasanta Tipparat Kongseha Suchart Kothan | 2019 | World Journal of Radiology2019，11，1： | 0 |
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