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| 1 | Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81显示文摘AIM: To further analyze the interaction of tupaia CD81 with hepatitis C virus (HCV) envelope protein E2. METHODS: A tupaia CD81 large extracellular loop (CD81 LEL), which binds to HCV E2 protein, was cloned and expressed as a GST-fusion protein, and interaction of HCV E2 protein with a tupaia CD81 LEL was evaluated by enzyme-linked immunosorbent assay (EIA). RESULTS: Although tupaia and human CD81 LEL differed in 6 amino acid changes, tupaia CD81 LEL was strongly recognized by anti-CD81 antibodies against human CD81 LEL conformation-dependent epitopes. Investigating LEL CD81-E2 interactions by EIA, we demonstrated that binding of tupaia CD81 LEL GST fusion protein to recombinant HCV E2 protein was markedly reduced compared to binding of human CD81 LEL GST fusion protein to recombinant HCV E2 protein. CONCLUSION: These data suggest that the structural differences in-between the tupaia and human CD81 may alter the interaction of the large extracellular loop with HCV envelope glycoprotein E2. These findings may be important for the understanding of the mechanisms of binding and entry of HCV to PTHs. | Zhan-Fei Tian Hong Shen Xi-Hua Fu Yi-Chun Chen Hubert E Blum Thomas F Baumert Xi-Ping Zhao | 2009 | World Journal of Gastroenterology2009，15，2： | 16 |
| 2 | Hepatitis C virus infection and apoptosis显示文摘Apoptosis is central for the control and elimination of viral infections. In chronic hepatitis C virus (HCV) infection, enhanced hepatocyte apoptosis and upregulation of the death inducing ligands CD95/Fas, TRAIL and TNFα occur. Nevertheless, HCV infection persists in the majority of patients. The impact of apoptosis in chronic HCV infection is not well understood. It may be harmful by triggering liver fibrosis, or essential in interferon (IFN) induced HCV elimination. For virtually all HCV proteins, pro- and anti-apoptotic effects have been described, especially for the core and NS5A protein. To date, it is not known which HCV protein affects apoptosis in vivo and whether the infectious virions act pro- or anti- apoptotic. With the availability of an infectious tissue culture system, we now can address pathophysiologically relevant issues. This review focuses on the effect of HCV infection and different HCV proteins on apoptosis and of the corresponding signaling cascades. | Richard Fischer Thomas Baumert Hubert E Blum | 2007 | World Journal of Gastroenterology2007，13，36： | 10 |
| 3 | 建立广州生物库进行慢性重大疾病研究概述显示文摘目的报告《建立广州生物库进行慢性重大疾病研究》过去十年的研究进展和今后十年的研究展望。方法选择≥50岁的30 518名有广州市户籍的居民,收集其个人背景资料、职业接触、居住和生活环境与习惯、营养摄入及个人与家族史等详细资料,并作体格检查,血生化检验、尿柯铁宁及心电图、X线胸片与肺功能检测等,同时储存其白细胞与血浆在-196℃液态氮库内使其永生化;定期重复体检随访及电话随访并建立亚队列进行基因、生活方式与职业环境等因素对慢性重大疾病影响的研究。计数资料采用χ2检验,计量资料符合正态分布的采用t检验,不符合正态分布的采用秩和检验,以双侧P<0.05为差异有统计学意义。结果 (1)建立液态氮生物库、条码识别技术与Fico11-Pague分离细胞室及分子流行病学研究室的管理系统;(2)分三个阶段收集30 518名研究对象建立生物库中老年队列;(3)基线资料分析表明,'三高'凸显,男女血压高值分别为24.0%和17.8%,高血压患病率分别为45.3%和41.3%,χ2值为39.89,P<0.001;血糖高值和糖尿病患者男性分别为11.1%和12.0%,略高于女性的9.9%和12.6%,χ2值分别为9.00和2.04,P=0.191和0.293,差异无统计学意义;而高脂血症更为明显,检出率则女性明显高于男性;(4)中央型肥胖、超重和全身性肥胖以及代谢综合征也呈现女性高于男性;短期记忆受损则男性为13.9%,稍高于女性的12.7%,χ2值为8.05,P=0.005;(5)发现不良的生活方式如吸烟、二手烟、饮酒、缺乏体力活动、不良睡眠状态及打鼾对健康的影响,经历不同社会经济环境(生命历程)成长发育对健康的影响,研究已发表论文80多篇;(6)第一次重复体检和电话随访已于2012年12月结束,除606名研究对象因户口迁出或身份证有误无法确定外,成功随访率达98.0%,已知1 801例死亡,随访资料仍在处理和分析中。结论既往十年《广州生物库中老年队列研究》项目已建立标准化的研究平台及完成第一次重复体检与电话随访,未来十年将继续重复体检为主的纵向随访及建立亚队列进行基因、职业与环境因素及生活方式对慢性重大疾病影响的研究,不断向深度和广度发展。 | 江朝强 林大庆 郑家强 刘斌 张维森 靳雅丽 徐琳 Kin Bong Hubert Lam Graham Neil Thomas Peymane Adap 朱彤 梁卓伟 | 2013 | 中国预防医学杂志2013，14，11： | 6 |
| 4 | Pegylated interferon alfa-2b alone or in combination with lamivudine for HBeAg-positive chronic hepatitis B: a randomised trial显示文摘 | Harry LA Janssen Monika van Zonneveld Hakan Senturk Stefan Zeuzem Ulus S Akarca Yilmaz Cakaloglu Christopher Simon Thomas MK So Guido Gerken Robert A de Man Hubert GM Niesters Pieter Zondervan Bettina Hansen Solko W Schalm | 2005 | The Lancet . 2005 (9454)2005，，： | 2 |
| 5 | Immunodominance and functional alterations of tumor‐associated antigen‐specific CD8+ T‐cell responses in hepatocellular carcinoma显示文摘 | Tobias Flecken Nathalie Schmidt Sandra Hild Emma Gostick Oliver Drognitz Robert Zeiser Peter Schemmer Helge Bruns Thomas Eiermann David A. Price Hubert E. Blum Christoph Neumann‐Haefelin Robert Thimme | 2014 | Hepatology2014，，： | 2 |
| 6 | Serotonin type 3 receptor subunit gene polymorphisms associated with psychosomatic symptoms in irritable bowel syndrome:A multicenter retrospective study显示文摘BACKGROUND Single-nucleotide polymorphisms(SNPs)of the serotonin type 3 receptor subunit(HTR3)genes have been associated with psychosomatic symptoms,but it is not clear whether these associations exist in irritable bowel syndrome(IBS).AIM To assess the association of HTR3 polymorphisms with depressive,anxiety,and somatization symptoms in individuals with IBS.METHODS In this retrospective study,623 participants with IBS were recruited from five specialty centers in Germany,Sweden,the United States,the United Kingdom,and Ireland.Depressive,anxiety,and somatization symptoms and sociodemographic characteristics were collected.Four functional SNPs—HTR3A c.-42C>T,HTR3B c.386A>C,HTR3C c.489C>A,and HTR3E c.*76G>A—were genotyped and analyzed using the dominant and recessive models.We also performed separate analyses for sex and IBS subtypes.SNP scores were calculated as the number of minor alleles of the SNPs above.The impact of HTR3C c.489C>A was tested by radioligand-binding and calcium influx assays.RESULTS Depressive and anxiety symptoms significantly worsened with increasing numbers of minor HTR3C c.489C>A alleles in the dominant model(F_(depressive)=7.475,P_(depressive)=0.006;F_(anxiety)=6.535,P_(anxiety)=0.011).A higher SNP score(range 0-6)was linked to a worsened depressive symptoms score(F=7.710,P-linear trend=0.006)in IBS.The potential relevance of the HTR3C SNP was corroborated,showing changes in the expression level of 5-HT3AC variant receptors.CONCLUSION We have provided the first evidence that HTR3C c.489C>A is involved in depressive and anxiety symptoms in individuals with IBS.The SNP score indicated that an increasing number of minor alleles is linked to the worsening of depressive symptoms in IBS. | Sabrina Berens Yuanjun Dong Nikola Fritz Jutta Walstab Mauro D'Amato Tenghao Zheng Verena Wahl Felix Boekstegers Justo Lorenzo Bermejo Cristina Martinez Stefanie Schmitteckert Egbert Clevers Felicitas Engel Annika Gauss Wolfgang Herzog Robin Spiller Miriam Goebel-Stengel Hubert Mönnikes Viola Andresen Frieling Thomas Jutta Keller Christian Pehl Christoph Stein-Thöringer Gerard Clarke Timothy G Dinan Eamonn M Quigley Gregory Sayuk Magnus Simrén Jonas Tesarz Gudrun Rappold Lukas van Oudenhove Rainer Schaefert Beate Niesler | 2022 | World Journal of Gastroenterology2022，28，21： | 2 |
| 7 | Pilot trial of autologous dendritic cells loaded with tumor lysate(s)from allogeneic tumor cell lines in patients with metastatic medullary thyroidcarcinoma显示文摘 | Thomas Bachleitner-Hofmann Josef Friedl Michaela Hassler Hubert Hayden Peter Dubsky Monika Sachet Erwin Rieder Roswitha Pfragner Christine Brostjan Stefan Riss Bruno Niederle Michael Gnant Anton Stift | 2009 | Oncology Reports2009，，6： | 2 |
| 8 | Pseudomonas exotoxin antisense RNA selectively kills hepatitis B virus infected cells显示文摘AIM: To present an approach for selectively killing retrovirus-infected cells that combines the toxicity of Pseudomonas exotoxin (PE) and the presence of reverse transcriptase (RT) in infected cells. METHODS: PE antisense toxin RNA has palindromic stem loops at its 5' and 3' ends enabling self-primed generation of cDNA in the presence of RT. The RT activity expressed in retrovirus-infected cells converts 'antisense-toxin-RNA' into a lethal toxin gene exclusively in these cells. RESULTS: Using cotransfection studies with PE-expressing RNAs and β-gal expressing reporter plasmids, we show that, in HepG2 and HepG2.2.15 hepatoma cells as well as in duck hepatitis B virus (DHBV) infected cells, HBV or DHBV-polymerase reverse transcribe a lethal cDNA copy of an antisense toxin RNA, which is composed of sequences complementary to a PE gene and eukaryotic transcription and translation signals. CONCLUSION: This finding may have important implications as a novel therapeutic strategy aimed at the elimination of HBV infection. | Peter Hafkemeyer Ulrich Brinkmann Elizabeth Brinkmann Ira Pastan Hubert E Blum Thomas F Baumert | 2008 | World Journal of Gastroenterology2008，14，18： | 2 |
| 9 | Pegylated interferon alfa-2b alone or in combination with lamivudine for HBeAg-positive chronic hepatitis B: a randomised trial显示文摘 | Harry LA Janssen Monika van Zonneveld Hakan Senturk Stefan Zeuzem Ulus S Akarca Yilmaz Cakaloglu Christopher Simon Thomas MK So Guido Gerken Robert A de Man Hubert GM Niesters Pieter Zondervan Bettina Hansen Solko W Schalm | 2005 | 2005 (9454)2005，，9454： | 1 |
| 10 | Preclinical efficacy and safety of rVIII-SingleChain (CSL627), a novel recombinant single-chain factor VIII显示文摘 | Sabine B. Zollner Elmar Raquet Jochen Müller-Cohrs Hubert J. Metzner Thomas Weimer Ingo Pragst Gerhard Dickneite Stefan Schulte | 2013 | Thrombosis Research2013，，： | 1 |
| 11 | Short‐term versus long‐term induction therapy with antithymocyte globulin in orthotopic liver transplantation显示文摘 | Thomas Soliman Hubert Hetz Christoph Burghuber Georg Gy?ri Gerd Silberhumer Rudolf Steininger Ferdinand Mühlbacher Gabriela A. Berlakovich | 2007 | Transplant International2007，，5： | 1 |
| 12 | Virus-host Interactions during Hepatitis C Virus Entry-Implications for Pathogenesis and Novel Treatment Approaches显示文摘Hepatitis C virus (HCV) is a member of the Flaviviridae family and causes acute and chronic hepatitis. Chronic HCV infection may result in severe liver damage including liver cirrhosis and hepatocellular carcinoma. The liver is the primary target organ of HCV, and the hepatocyte is its primary target cell. Attachment of the virus to the cell surface followed by viral entry is the first step in a cascade of interactions between the virus and the target cell that is required for successful entry into the cell and initiation of infection. This step is an important determinant of tissue tropism and pathogenesis; it thus represents a major target for antiviral host cell responses, such as antibody-mediated virus neutralization. Following the development of novel cell culture models for HCV infection our understanding of the HCV entry process and mechanisms of virus neutralization has been markedly advanced. In this review we summarize recent developments in the molecular biology of viral entry and its impact on pathogenesis of HCV infection, development of novel preventive and therapeutic antiviral strategies. | Joachim Lupberger Mirjam B. Zeisel Anita Haberstroh Eva K. Schnober Sophie Krieger Eric Soulier Christine Thumann Cathy Royer Samira Fafi-Kremer Catherine Schuster Franoise Stoll-Keller Hubert E. Blum Thomas F. Baumert | 2008 | Virologica Sinica2008，23，2： | 1 |
| 13 | Intravenous Contrast Medium Aggravates the Impairment of Pancreatic Microcirculation in Necrotizing Pancreatitis in the Rat显示文摘 | Jan Schmidt Hubert G. Hotz Thomas Foitzik Eduard Ryschich Heinz J. Buhr Andrew L Warshaw Christian Herfarth Ernst Klar | 1995 | Annals of Surgery1995，，3： | 1 |
| 14 | Technology Development Mode:a Transaction Cost Conceptualization显示文摘 | THOMAS S R HUBERT G | 1998 | Strategic Management Joumal1998，，： | 1 |
| 15 | Antireflux Transoral Incisionless Fundoplication Using EsophyX: 12-Month Results of a Prospective Multicenter Study显示文摘 | Guy-Bernard Cadière Michel Buset Vinciane Muls Amin Rajan Thomas R?sch Alexander J. Eckardt Joseph Weerts Boris Bastens Guido Costamagna Michele Marchese Hubert Louis Fazia Mana Filip Sermon Anna K. Gawlicka Michael A. Daniel Jacques Devière | 2008 | World Journal of Surgery2008，，8： | 1 |
| 16 | An Exchange Theory Model of Interpersonal Communication 显示文摘 | Garignon Hubert Thomas S Robertson | 1986 | Advances in Consumer Re- search1986，13，： | 1 |
| 17 | Are self-thinning constraints needed in a tree-specific mortality model?显示文摘 | MONSERUD R A THOMAS L HUBERT S | 2004 | Forest Science2004，50，6： | 1 |
| 18 | An Exchange Theory Model of Interpersonal Communication 显示文摘 | Gatignon Hubert and Robertson Thomas S | 1986 | Advances in Consumer Re- search1986，13，： | 1 |
| 19 | Pegylated interferon alfa-2b alone or in combination with lamivudine for HBeAg-positive chronic hepatitis B: a randomised trial显示文摘 | Harry LA Janssen Monika van Zonneveld Hakan Senturk Stefan Zeuzem Ulus S Akarca Yilmaz Cakaloglu Christopher Simon Thomas MK So Guido Gerken Robert A de Man Hubert GM Niesters Pieter Zondervan Bettina Hansen Solko W Schalm | 2005 | The Lancet2005，，9454： | 1 |
| 20 | Fire retardancy of sol-gel derived titania wood-inorganic composites显示文摘 | Muhammad Shabir Mahr Thomas Hubert Martin Sabel | 2012 | Mater Sci2012，47，： | 1 |
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