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| 1 | New method for the analysis of cell cycle-specific apoptosis显示文摘 | Tao Deding Wu Jianhong Feng Yongdong | 2004 | Cytometry Part A2004，57，： | 1 |
| 2 | New Method for the Analysis of Cell Cycle - Specific Apoptosis 显示文摘 | Tao Deding Wu Jianhong Feng Yongdong | 2004 | Cytometry Part A2004，7074，： | 1 |
| 3 | Analysis of DNA ploidy, cell cycle and Ki67 antigen in nasopharyngeal carcinoma by flow cytometry显示文摘 | Shi Xiaoyan Yuan Xianglin Tao Deding Gong Jianping Hu Guoqing | 2005 | Journal of Huazhong University of Science and Technology [Medical Sciences]2005，，： | 1 |
| 4 | CDK1、CDK2 siRNA干扰对肿瘤细胞凋亡和细胞周期的影响(英文)显示文摘Objective:We investigated the influence of CDK1 and CDK2 expression inhibited by cotransfection of CDK1 and CDK2 siRNA on cell cycle and apoptosis,explored the exact role of cell cycle master regulator in tumor cell apoptosis process.Methods:The siRNA targeting the CDK1 and CDK2 genes were synthesized and simultaneously cotransfected into Hela cells by lipofectamine 2000.48 or 60 h after the cotransfection,CDK1 and CDK2 protein expressions were examined by Western blot.Cell cycle distribution was analyzed by flow cytometry.Cell apoptosis was detected by the Annexin V/PI method.The changes of the transfected cell morphological under a microscope after Wright-Giemsa Staining were studied.Results:CDK1 and CDK2 protein expression was decreased at 48 or 60 h after cotransfection.The accumulation of the G2/M and S phase population in cell cycle of the cotransfected cells at 48 or 60 h after transfection was enhanced obviously compared with control.The ratio of apoptotic cell of cotransfected cells at 48 or 60 h after transfection was increased significantly compared with control.More binucleate or multinucleate cells among cotransfected cells were observed under the microscope.Conclusion:The decreased expression of CDK1 and CDK2 by cotransfection of CDK1 and CDK2 siRNA not only leads to tumor cell cycle arrest in S phase and G2/M phase,but also induces tumor cell apoptosis. | Hui Xiao Wanjun Gong Jingpeng Cao Xiaolan Li Deding Tao Junbo Hu Jianping Gong | 2009 | The Chinese-German Journal of Clinical Oncology2009，8，7： | 1 |
| 5 | γ-H2AX细胞周期相关性的分析(英文)显示文摘Objective:To analyze and discuss cell cycle's correlation of γ-H2AX,so as to accumulate the data for the further studies of γ-H2AX.Methods:MOLT-4 cells,and peripheral blood lymphocytes(PBLs),with or without 48 h stimulation of phytohemagglutinin(PHA),were irradiated by ultraviolet rays(UV rays).Fluorescence-labeled γ-H2AX antibody was used to detect γ-H2AX foci at the DNA double-strand breaks(DSBs) in chromatin,DNA damage was analyzed by flow cytometry,cell cycle and cell apoptosis were detected by sub-G1 peak method,the expression of γ-H2AX was detected by Western blot.Results:With the progression of time,sub-G1 peak emerged apparently in the DNA histograms,and the cells of apoptosis increased gradually;with the progression of time,the increase of γ-H2AX emerged and firstly raised,then decreased;PBLs with 48 h stimulation of PHA entered apparently cell cycle,cells of S and G2/M phase emerged,and PBLs without stimulation of PHA did not enter cell cycle;Western blot showed the increase of the expression of γ-H2AX,and the increase also firstly raised,then decreased.Conclusion:γ-H2AX expressed in the cells of stationary phase and proliferative phase,and with the progression of time,the increase of γ-H2AX firstly raised,and then decreased. | Yangping Yue Zhenchuang Zhu Dongdong Yu Yu Deng Dan Huang Xiaolan Li Wei Xiao Deding Tao Junbo Hu Jianping Gong | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，10： | 0 |
| 6 | 紫外线诱导的Molt-4细胞旁观者效应研究(英文)显示文摘Objective: To find out whether ultraviolet ray, a kind of non-ionic ray, could cause the bystander effect, the UV exposed MOLT-4 cells had been investigated. Methods: Two experiment groups were carried out, in which cells were culture and treated at two concentrations: 2 x 105/mL and 5 x 105/mL. All other treatments were the same. Part of the cells was labeled with DID and exposed to UV ray for 40 s as effect cells; other cells was untreated as bystander cells. Then, the cells were co-cultured and harvested at 4 h interval over a period of 24 h. Annexin V-FITC/PI assay was used to evaluate the bystander effect in bystander cells co-cultured with effected cells. Laser confocal microscope method was used to observe the morphologic changes of the bystander cells. Results: The percentage of cells undergoing apoptosis in the bystander cells was increased over time compared with the control group. They were 6.84%, 8.09%, 9.88%, 17.64%, 17.43%, 30.99% and 37.93% respectively in 0 h, 4 h, 8 h, 12 h, 16 h, 20 h and 24 h. When observed by laser scanning confocal microscope, the bystander cells show some classic character of apoptosis such as chromosome condense, phosphatidylserine transfer and formation of apoptotic bodies. Conclusion: Bystander effect is significant in un-irradiated bystander MOLT-4 cells when co-cultured with UV exposed cells. | Dan Huang Tian Xiao Xiangshang Xu Deding Tao Junbo Hu Jianping Gong | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，4： | 0 |
| 7 | Basal与非basal亚型三阴性乳腺癌临床病理及预后相关因素分析(英文)显示文摘Objective:Triple-negative breast cancer(estrogen receptor-negative,progesterone receptor-negative and Her2-negative) can be classified into two subtypes:basal and non-basal phenotype.And the basal phenotype is associated with poor outcome.The purpose of this study was to figure out the differences of clinicopathological characters and related factors of prognosis between these two subtypes.Methods:Immunohistochemical staining was performed for the CK5/6,CK17 basal markers and EGFR on biopsy samples from 40 triple-negative patients and the clinicopathology features of these samples were investigated.Results:Seventy percent of the patients were diagnosed as the basal phenotype.Compared with the non-basal phenotype,the basal phenotype lesions were significantly larger in diameter with a high nuclear grade.In the node-negative group the basal phenotype clearly showed the same clinicopathological differences.There was statistically significant concordance among all three antibodies.Conclusion:Expression of basal markers identifies a biologically and clinically distinct subgroup of TN tumors,justifying the use of basal markers to define the basal or the non-basal phenotype.It is important to help the doctor deciding the therapeutic strategy for patient with triple-negative breast cancer. | Lin Sun Lin Zhang Shasha Ren Deding Tao Yaqun Wu | 2010 | The Chinese-German Journal of Clinical Oncology2010，9，5： | 0 |
| 8 | The significance of cyclin B1 expression in colorectal cancers显示文摘Objective: To study the relationship between the expression of human cyclin B1 in colorectal carcinomas and the pathological characters. Methods: The Expression of cyclin B1 in 66 cases of colorectal carcinomas were detected by flow cytometry and immunohistochemistry. Then the relationship between the expression of cyclin B1 in colorectal carcinomas and pathological characters was analyzed with statistics. Results: The expression of cyclin B1 in colorectal carcinomas had associa- tivity with the cancer cell differentiation (P<0.05); However, the expression of cyclin B1 in colorectal carcinomas had no obvious associativity with cancer cell infiltrate depth and lymph nodes metastasis (P>0.05). Conclusion: In the colorectal cancers with high expression of cyclin B1, the cancer cells would present high differentiation; with low expression of cyclin B1 the cancer cells would present low differentiation. Along with the expression of cyclin B1 from high to low, the cancer cells differentiation has the tendency from high to low too. | Haocheng Long Xia Gao Xiaoyan Chen Xiaolan Li Xiaojun He Deding Tao Jianping Gong | 2006 | The Chinese-German Journal of Clinical Oncology2006，5，6： | 0 |
| 9 | Cytarabine and paclitaxel exhibit different cell-cycle specificities in different cell growing status显示文摘Objective:To investigate the cell-cycle specificities of cytarabine and paclitaxel in different growing status of target cell.Methods:Using flow cytometry,we tested the cell-cycle specificities of cytarabine and paclitaxel on acute lymphocyte leukemia cell line Molt-4 in different growing status and on clinical acute lymphocyte leukemia specimens in vitro as well as in leu-kemia patients in vivo.Results:Cytarabine induced S phase specific cell-cycle blockage and apoptosis in exponentially growing Molt-4,but showed G0/G1 phase specificity in high-density cultured Molt-4 and in clinical specimens.Paclitaxel induced G2/M phase specific cell-cycle blockage and apoptosis in exponential Molt-4,but showed G0/G1 phase specificity in high-density cultured Molt-4 and S phase specificity in clinical specimens.In the first day of clinical chemotherapy,cytarabine induced G0/G1 with a little S phase apoptosis in leukemia cells of acute lymphocyte leukemia patient in vivo.Cytarabine plus paclitaxel together had almost the same effect in the second day.Conclusion:The cell-cycle effects of cytarabine and paclitaxel were different in different target cell growing status.It should be noted that the in vivo effect of these agents may be different from people usually anticipated during clinical chemotherapy.So the combined chemotherapeutic regimens may need to be redesigned. | Peng Zhang Yi Zhou Deding Tao Jianfeng Zhou Jianping Gong | 2006 | The Chinese-German Journal of Clinical Oncology2006，5，6： | 0 |
| 10 | 含异倍体的结直肠癌高表达CD133且预后不良(英文)显示文摘Objective:The aim of the study was to investigate the relationship of DNA ploidy status in colorectal cancers with patients' prognosis and also the relationship of DNA ploidy status with expression of the colorectal cancer stem cell marker CD133.Methods:The DNA ploidy status and CD133 expression in colorectal cancers were detected by flow cytometry.The clinicopathological characteristics and progression-free survival analysis of patients was evaluated based on the clinical data.Results:DNA ploidy pattern did not correlated with gender,age,lesion diameter,histological type,depth of tumor invasion,lymphatic invasion and Dukes stage.Only primary lesion cite showed significant correlation with DNA ploidy pattern,more aneuploids were observed in colonic cancer than rectal cancer,P < 0.05.The 2-year progression-free survival rate and total progression-free time in patients with aneuploids were lower than that with diploids,P < 0.05.Tumors contained aneuploids showed higher expression of CD133 than tumors of only diploids,P < 0.05.Conclusion:Tumor DNA ploidy status is a significant prognostic factor in patients with colorectal cancer and also associated with the existence of CD133 positive colorectal cancer stem cells. | Dongdong Yu Yonghong Zhang You Zou Ming Tian Deding Tao Junbo Hu Jianping Gong | 2010 | The Chinese-German Journal of Clinical Oncology2010，9，10： | 0 |
| 11 | The role of CDK1 siRNA interference in cell cycle and cell apoptosis显示文摘In the present report,cyclin-dependent kinase1(CDK1)siRNA was transfected into cells to silence the CDK1 gene expression and study its role in the cell cycle and cell apoptosis.The siRNA targeting CDK1 gene was chemically synthesized and transfected into Hela cells by lipofectamine 2000.The expression levels of CDK1 gene and protein were examined by real-time quantitative polymerase chain reaction(PCR)and Western blot,respectively.The cell cycle was analyzed by using DNA content analysis byflow cytometry.Cell apoptosis was detected by the Annexin V/PI method.The morphological changes of transfected cells were examined under the microscopy by Wright-Giemsa stain.CDK1 gene was successfully silenced by its siRNA,and the CDK1 protein expression level was decreased significantly,especially from 48th h to 60th h after transfection.The DNA content analysis showed that transfection of CDK1 siRNA led to cells accumulating in G2/M phase.There was no significant difference in the apoptotic rate between transfected cells and the control cells after transfection of CDK1 siRNA for 48 or 60 h.More double nucleus or multinucleus cells could be seen under the microscopy among the transfected cells.The decreased CDK1 expression by siRNA silencing gave rise to cell cycle arrest in G2/M phase but did not induce apoptosis. | Hui XIAO Ming TIAN Junna GE Xin WEI Zhaoming LI Xiaolan LI Deding TAO Junbo HU Jianping GONG | 2009 | Frontiers of Medicine2009，3，4： | 0 |
| 12 | The feasibility of antitumor drugs chemosensitivity testing by flow cytometry显示文摘Objective: To investigate the feasibility of chemosensitivity testing of antitumor drugs by flow cytometry in clinical applications so as to provide experimental and theoretical basis for the establishment of a novel antitumor drugs sensitivity testing and the screening of particular antitumor drugs. Methods: Detect the apoptosis rate of 12 cases of Molt-4 cell line, 57 cases of fresh clinical gastrointestinal tumor cells by Sub-G1 and Annexin V assay of flow cytometry under the effects of antitumor drugs at different times and the outcomes were compared with the ones of the MTT (3-(4,5-dimethylthiazolyl-2) -2,5-diphenyltetrazolium bromide) assay. Results: The lethality of drugs on Molt-4 cell, clinical gastrointestinal tumor cells had a positive correlation with the acting time of antidrugs by employing Annexin V, Sub-G1 and MTT assay. Drug-incurring maximum lethality of Annexin V assay was higher than MTT colorimetric assay, that of Sub-G1 was lower than MTT assay, the virtual times of Annexin V and Sub-G1 assay were obviously earlier than that of MTT colorimetric assay. Conclusion: Annexin V and Sub-G1 assay of flow cytometry can be taken as potent protocols testing anti-tumor drug chemosensitivity. Annexin V assay is featured by more sensitive, concise, reliable compared with the classical chemosensitivity testing assay of MTT colorimetric assay and it possesses clinical applied value. | Jing Yao Jianhong Wu Daxing Xie Xiaolan Li Deding Tao Junbo Hu Jianping Gong | 2007 | The Chinese-German Journal of Clinical Oncology2007，6，3： | 0 |
| 13 | Dual effect of Molt-4 cell line onset with roscovitine显示文摘Objective:To observe the effect of roscovitine(ROSC)as some Cyclin-dependent kinases(CDKs),inhibitor onset with the Molt-4 cell lines.Methods:DNA assay of single cells by flow cytometry was used to detect the effect of cell cycle arrest and Annexin-V/FITC assay was used to detect the effect of cell apoptosis when the Molt-4 cell lines cultured with different concentration ROSC in different time points.Results:It showed that ROSC exerted strong inhibitory effect on prolif- eration and cell cycle progression of Molt-4 cell lines,accumulation of G2/M cells arrested took place after onset with 10μm and 20μm ROSC more than 6 h;at the same time,the cell apoptosis of Molt-4 cells would be detected.According with the time and concentration increasing,the cell apoptosis rate would rise.Conclusion:It is concluded that Roscovitine(ROSC) as some Cyclin-dependent kinases(CDKs),inhibitor,has dual effects to Molt-4 cells,not only the effect of cell cycle arrest but also the effect of cell apoptosis. | Haocheng Long Xia Gao Xiaolan Li Hui Xiao Xiaojun He Deding Tao Jianping Gong | 2007 | The Chinese-German Journal of Clinical Oncology2007，6，4： | 0 |
| 14 | 结肠癌细胞系SW480对不同化疗药物化疗敏感性的研究(英文)显示文摘Objective:The aim of this study was to investigate the sensitivity of chemotherapeutic agents 5-FU,cisplatin(DDP) or TAXOL on colon cancer cell line SW480 with different methods,to find out the best examine time period for further study of chemotherapeutic sensitivities.Methods:The SW480 cell was treated with 5-FU(200μg/mL),DDP(150μg/mL) or TAXOL(50μg/mL) respectively for 4h,8h or 12h.MTT assay was used to examine the cell survival rate,Annexin V/PI assay was used to analysis the apoptosis rate,Western Blot assay was applied to examine the expression of apoptotic protein.Results:(1) Results of MTT assay showed that the survival rate of SW480 cells at 4h,8h or 12h was:5-FU(200μg/mL)96.0%±8.2%,85.4%±7.8%,74.4% ±10.2%(P<0.05);DDP(150μg/mL) 99.0%±6.4%,88.7%±4.7%(P<0.05),46.9%±2.6%(P<0.01);TAXOL(50μg/mL) 51.5%±4.2%(P<0.01),31.9%±3.1%,17.6%±2.3%,or blank group 97.2%±5.8%,98.7%±7.2%,97.5%±7.5% respectively.(2) The apoptosis rate of cancer cells at 4h,8h or 12h was:control group:3.4%±0.2%,6.2%±0.4%,7.0%±0.5%;5-FU(200μg/mL) 4.0%±0.3%,4.8%±0.4%,7.7%±0.7%;DDP(150μg/mL) 8.5%±0.9%,18.6%±1.6%(P<0.05),67.0%±6.2%(P<0.01);or TAXOL(50μg/mL) 32.0%±5.2%(P<0.01),76.6%±8.5%,94.0% ±8.2%,respectively.(3) Western Blot assay showed that the expression of apoptosis associated protein.PARP,X-linked inhibitor of apoptasis(XIAP),Caspase-9 and Bcl-xL were changed.Conclusion:The sensitivity of chemotherapy could be assessed by MTT assay,Annexin V/PI assay and Western Blot.The best examine time of the three chemotherapuc agents was 5-FU(200μg/mL):>12h,DDP(150μg/mL):8-12h,or TAXOL(50μg/mL):<4h. | Yu Deng Guihua Wang Weina Li Xiaolan Li Wei Xiao Deding Tao Jianping Gong Junbo Hu | 2011 | The Chinese-German Journal of Clinical Oncology2011，10，5： | 0 |
| 15 | 金克诱导Molt-4细胞凋亡及其机制研究(英文)显示文摘Objective: To investigate the apoptosis-inducing effect of Jinke on Molt-4 cells and its possible mechanism. Methods: The Molt-4 cells were treated with different concentrations of Jinke and then cultured for necessary time. The An- nexin-V / PI method was used to detect the apoptosis rate. The cell cycle was analyzed by DNA content with flow cytometry. Double parameters analysis of cyclins / DNA was performed to detect the expression of cyclin E. API method was used to confirm the cell cycle-specific apoptosis. The expressions of Bcl-2 and Bax were detected by western blot. Results: 24 h after the treatment of 0.5, 1.0, 1.5, 2.0 and 3.0 mg/mL Jinke, the apoptosis rate of Molt-4 cells was evaluated in a concentra- tion-dependent manner, from 5.2% of the control group to 41.0% of the 3.0 mg/mL Jinke group. When the Molt-4 cells were cultured with 1.5 mg/mL Jinke, the apoptosis rate was evaluated in a time-dependent manner. DNA content analysis showed that G0/G1 phase of Molt-4 cells increased in a time-dependent manner. The expression of cyclin E increased gradually. API assay showed the apoptosis cells were almost in G0/G1 phase. Western blot showed the Bcl-2 was down-regulated and the Bax was up-regulated. Conclusion: Jinke could induce G1 phase-specific apoptosis in Molt-4 cells in time- and concentra- tion-dependent manners involving G1 phase arrest. The mechanism of apoptosis inducing effect may be related to the up- regulation of Bax and the down-regulation of Bcl-2. | Zhenchuang Zhu Yixin Tong Yangping Yue Yu Deng Dongdong Yu Wei Xiao Xiaolan Li Deding Tao Junbo Hu Jianping Gong | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，8： | 0 |
| 16 | Epidermal growth factor enhances chemosensitivityof colon cancer by inducing cancer stem cells toenter the cell cycle显示文摘Objective The aim of the study was to investigate whether colon cancer stem cells induced by epidermal growth factor(EGF) to enter the cell cycle enhanced the chemosensitivity of colon cancer.Methods In vitro,EGF was used to stimulate the entry of human colon cancer HCT116 cells into the cell cycle.Before and after treatment with EGF,CD133+ HCT116 cells were collected and flow cytometry was conducted to determine the apoptosis rate based on the 5-Fu and Ki-67 expression rates.The cell cycle distribution of the two groups was also determined.In vivo,a subcutaneous xenograft model of HCT116 human colon cancer cell lines in nude mice was established.The nude mice were divided into two groups and treated with EGF and 5-Fu,respectively.Differences in the growth of implanted tumors revealed the efficiency of cycle-induction combined chemotherapy.Results(1) After EGF stimulation,the S-G2/M proportion of CD133+ HCT116 cells and Ki67 expression were increased,indicating that more cancer stem cells entered the cell cycle and promoted proliferation;(2) After EGF stimulation,CD133+ HCT116 cells showed a higher apoptosis rate induced by 5-Fu.(3) Animal experiments showed that the group subjected to combined treatment with EGF and 5-Fu had smaller tumor sizes compared to the group treated with 5-Fu alone.Conclusion EGF enhanced tumor sensitivity to chemotherapeutic drugs,likely by promoting tumor stem cells to enter the cell cycle. | You Zou Xiaolan Li Deding Tao Junbo Hu Jianping Gong | 2017 | Oncology and Translational Medicine2017，3，2： | 0 |
| 17 | CDK1在紫外线诱导的细胞凋亡中重要作用的研究(英文)显示文摘Objective:To find out whether CDK1 could appear the important role in the cell apoptosis that induced by ultraviolet ray.Methods:We constructed two kinds of plasmids, one can expressed CDK1 but mutated the fifth amino acid(group A ) and the other can effectively silence the expression of CDK1(group B).When we transfected these plasmids into the 293 cells and at the same time following ultraviolet radiation, we detected the difference of two group cells' apoptosis ratio by subG1 peak assay and want to clarify the mechanism by Western blot assay.Results:Two kinds of plasmids could effectively express in 293 cells.The apoptosis ratio of group A was higher than the group B(P < 0.01).And the apoptosis ratio of group B was lower than the group C(P < 0.01).Compared with group A and C, the expression of Bcl2 of group B was significantly reduced.Conclusion:CDK1 may take a very important role in the programmed cell death. | Xin Wei Leya He Xiaolan Li Hui Xiao Deding Tao Junbo Hu Jianping Gong | 2009 | The Chinese-German Journal of Clinical Oncology2009，8，5： | 0 |
| 18 | The approaches in detecting cell cycle specificity of Fas-mediated apoptosis in leukemia cell lines and activated PBLs in vitro显示文摘Objective: To establish a system in detecting the cell cycle specificity induced by recombinant human Fas ligand in vitro, so as to provide a reliable platform for further exploring the mechanism of cell cycle control and regulation in Fas-medi- ated apoptosis. Methods: The target cells—leukaemia cell lines and activated peripheral blood lymphocytes stimulated by phytohemagglutinin were incubated with recombinant human Fas ligand for 6 to 36 h, apoptosis was detected by sub-G1, com- mon annexin-V/PI and modified annexin V and propidium iodide (API) methods and analysed by flow cytometry. Results: The modified API method demonstrated that Fas-mediated apoptosis was cell cycle specific and initiated at G1 phase. The common annexinV/PI method showed the most appropriate condition for the detection of typical cell cycle-specific apoptosis. The sub-G1 method could only illuminate late apoptosis and DNA histogram. Conclusion: Fas-mediated apoptosis was cell cycle-specific and initiated at G1 phase. Based on the modified API and common AnnexinV/PI methods, the establishment of stable and typical cell cycle-specific model in Fas-mediated apoptosis in vitro was feasible. | Xiaojun He Jing Hu Xiaolan Li Hui Xiao Deding Tao Haocheng Long Jianping Gong | 2006 | The Chinese-German Journal of Clinical Oncology2006，5，5： | 0 |
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