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| 1 | Functional analysis of the larval serum protein gene promoter from silkworm,Bombyx mori.显示文摘The regulation region of larval serum protein gene, Bombyx mori. (BmLSP), consisting of the first intron, the first exon, the central promoter region and 5′-upstream region, is cloned from genomic DNA from the silkworm va-riety of Suju譓inghu. Using PCR and restriction endonu-clease methods, a series of luciferase reporter plasmids, driven by different length of BmLSP promoters, are con-structed. Via the transient expression system in BmN cells, the effects of the regulation elements and foreign insect hor-mones on the BmLSP promoter activity are investigated. The results demonstrate that the promoter activity of BmLSP is 5.8- or 4.4-fold higher than that of BmLSPs whose first in-tron or the element in 5′-upstream region harboring the homologous sequence with the first intron of light-chain fib-roin gene (EHIF) is deleted, respectively, suggesting that both the first intron and EHIF contain the main positive cis-acting elements. However, the inactive mariner transposable ele-ment (MTE) in 5′-upstream region presents a negative effect. Furthermore, the effects of juvenile hormone analogue (JHA) on the BmLSP promoter activity show a typical dose-dependent manner, that is, low concentration treat-ments increase the BmLSP promoter activity and high con-centration treatments decrease it. Meanwhile, insect ecdy-sone (MH) treatments present no significant effect. | TANG Shunming, YI Yongzhu, SHEN Xingjia, ZHANG Zhifang, LI Yiren & HE Jialu Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sci-ences, Zhenjiang 212018, China Correspondence should be addressed to Zhang Zhifang (e-mail: zjsbsri@ public.zj.js.cn) | 2003 | Chinese Science Bulletin2003，48，23： | 13 |
| 2 | Interface and properties of epoxy resin modified by elastomeric nano-particles显示文摘Study on a new composite of epoxy resin/elastomeric nano-particles (ENP) is re- ported in this paper, which shows that, in comparison with pure epoxy resin and epoxy toughened with CTBN, the composites of epoxy resin/carboxylic nitrile-butadiene ENP and epoxy resin/styrene butadiene vinyl-pyridine ENP possess both higher toughness and heat resistance. Both ENPs used in the study have an average size of less than 100 nm. Study on the epoxy network’s morphology and interface properties suggests that due to the chemical reaction be- tween ENP and epoxy resin and more hydrogen bonds between nitrile groups of the rubber and hydroxyl groups of the epoxy resin, stronger interaction at the larger interface may lead to the observed excellent properties of the epoxy resin toughened with ENP. | HUANG Fan1, LIU Yiqun1, ZHANG Xiaohong1, GAO Jianming1, SONG Zhihai1, TANG Banghui1, WEI Genshuan2 & QIAO Jinliang1 1. SINOPEC Beijing Research Institute of Chemical Industry, Beijing 100013, China 2. Institute of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100080, China Correspondence should be addressed to Qiao Jinliang (email: jqiao@brici.ac.cn.) | 2005 | Science China Chemistry2005，48，2： | 9 |
| 3 | Interaction between en- dogenous nitric oxide and carbon monoxide in the pathogenesis of hypoxic pulmonary hypertension显示文摘The aim of the study was to investigate the in-teraction between nitric oxygenase (NOS)/ nitric oxide (NO) and heme oxygenase (HO)/ carbon monoxide (CO) system in the pathogenesis of hypoxic pulmonary hypertension. On a rat model of hypoxic pulmonary hypertension, the pulmo-nary artery pressure was measured, and NO formation and expression of NOS in pulmonary tissues were examined after treatment with ZnPP-IX, an HO-1 inhibitor. The pulmonary artery pressure, CO formation and expression of HO-1 in pulmonary tissues were examined after treatment with L-NAME, a NOS inhibitor. We found that pulmonary hy-pertension developed after 2-week hypoxia, while the con-centration of NO in the pulmonary tissue homogenates and the expression of NOS in intrapulmonary artery endothelial cells decreased markedly. ZnPP-IX worsened pulmonary hypertension of hypoxic rats. However, it increased endoge-nous production of NO and the expression of NOS obviously. The concentration of CO in the pulmonary tissue homoge-nates and the expression of HO-1 in intrapulmonary artery smooth muscle cells increased markedly with hypoxic pul-monary hypertension. L-NAME worsened pulmonary hy-pertension of hypoxic rats, but inhibited CO formation and HO-1 expression (P < 0.01). The results of this study sug-gested that endogenous NOS/NO and HO/CO systems might interact with each other and therefore play an important regulating role in hypoxic pulmonary hypertension. | SHI Yun, DU Junbao, GUO Zhiliang, ZENG Chaomei & TANG Chaoshu Department of Pediatrics, the First Hospital, Peking University, Beijing 100034, China Correspondence should be addressed to Du Junbao (e-mail: junbaodu@ ht.rol.cn) | 2003 | Chinese Science Bulletin2003，48，1： | 8 |
| 4 | Physiological responses of osteoblasts to cyclic stretching and the change of intracellular calcium concentration显示文摘The development of bone tissues is regulated by mechanical stimulation. Cyclic stretching was applied to the osteoblasts that were delivered from rat calvarie. The results showed that stretching at 500 me increased the cell prolifera-tion while loading at 1000 me and 1500 me inhabited cell growth. Loading also increased the adhesive force between cells and substrate as well as spreading areas of osteobalsts. Furthermore, the fluorescence probe Fluo-3/AM was used to investigate the effect of stretching stimulation on the intra-cellular calcium concentration of osteoblasts. The intracellu-lar calcium concentration of osteoblasts that were stretched at 500 me for 5 min was 92.9% higher than the control. After being treated with the panax ontoginseng saponins, the stretched osteoblasts still expressed 28.6% higher intracellu-lar calcium concentration than that of the control, which proved that both the influx of extracellular calcium and the release of intracellular calcium store were involved in the increase of intracellular calcium concentration when os-teoblasts responded to the cyclic stretching. And the influx of extracellular calcium through transmembrance channel played a main role. | TANG Liling, WANG Yuanliang, GU Li, CAI Shaoxi & SU Aihua Bio-engineering College, Chongqing University, Chongqing 400044, China Correspondence should be addressed to Tang Liling | 2003 | Chinese Science Bulletin2003，48，4： | 7 |
| 5 | The complete mitochondrial genomes sequences of Asio flammeus and Asio otus and comparative analysis显示文摘The complete mitochondrial genomes of Asio flammeus and Asio otus were se- quenced and found to span 18858 bp and 18493 bp, respectively. It is surprising to find the for- mer to be the largest among all avian mitochondrial genomes sequenced so far. The two ge- nomes have very similar gene order with that of Gallus gallus, neither contains the pseudo con- trol region, but both have a single extra base, namely Cytidine, at position 174 in ND3 gene. The control regions of Asio flammeus and Asio otus’ mitochondrial genomes span 3288 bp and 2926 bp respectively, which are the longest among vertebrates except for Myxine glutinosa and con- tribute to the large size of two genomes. The 3′end of the control region of Asio flammeus and Asio otus contains many tandemly repeated sequences, which are highly similar to a putative control element, i.e. Mt5, and may form stable stem-loop secondary structures. Such repeated sequences probably play an important role in regulating transcription and replication of mito- chondrial genome. Our results may provide important clues for uncovering the origin and evolu- tion mechanisms of mitochondrion genome. | SUN Yi1, MA Fei1, XIAO Bing1, ZHENG Junjie2, YUAN Xiaodong2, TANG Minqian2, WANG Li3, YU Yefei3 & Li Qingwei1 1. College of Life Sciences, Liaoning Normal University, Dalian 116029, China 2. TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian 116600, China 3. The Administrative Office of the Natural Protective Zone of Snake Island-Lao Tie Hill, Dalian 116041, China Correspondence should be addressed to Li Qingwei | 2004 | Science China(Life Sciences)2004，47，6： | 6 |
| 6 | Quasicrystalline Phase in As-cast Mg-Zn-Y-Zr Alloy显示文摘An icosahedral phase in as-cast Mg 5. 78Zn-0.89Y-0.48Zr (wt-%) alloy has been identified with transmission electron microscope (TPM) The selected area diffraction patterns (SADP) from the phase show a five-fold symmetry of a quasicrystalline structure. The energy dispersive X-ray spectra (EDXS) indicate that the quaternary phase has a chemical composition of 67.6Zn-1 9.4Mg-8,1 Y-4 9Zr in at -% | Yali TANG Ningfu SHEN and Xin YUAN (Research Center for Materials, Zhengzhou Institute of Technology, Zhengzhou, 450002, China)Zhiping LUO and Shaoqing ZHANG (Institute of Aeronautical Materials, Beijing,100095, China)(To whom correspondence should be ad | 1994 | Journal of Materials Science & Technology1994，10，4： | 4 |
| 7 | Ribozyme probe based on molecular beacon for real time monitoring of enzymatic cleavage process显示文摘Ribozyme probe based on molecular beacon (MBR) for monitoring enzymatic cleavage process in real time is designed and studied. The approach relies on ri-bozyme substrates modified at the two arms, with a fluores-cent moiety attached to the end of one arm and a non-fluorescent quenching moiety attached to the end of the other arm. MBR is employed to directly convert the cleavage information into fluorescence signal in real time. Compared with traditional approach, this method provides a no-radiolabeling, sensitive and effective way to research on the ribozyme activity, enzymatic dynamic process and ri-bozyme function during gene therapy. The activity of the ribozyme against hepatitis C virus RNA (HCV-RNA) is stud-ied based on this assay. | MENG Xiangxian, WANG Kemin, TAN Weihong, LI Jun, TANG Zhiwen, GUO Qiuping, HUANG Shasheng & LI Du State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Biological Technology, College of Chemistry and Chemical Engi-neering, Hunan University, Changsha 410082, China Correspondence should be addressed to Wang Kemin (e-mail: kmwang @hnu.net.cn) | 2003 | Chinese Science Bulletin2003，48，23： | 3 |
| 8 | Phase Diagram Prediction of the Al_2O_3-SiO_2-La_2O_3 System显示文摘Rare earth oxide systems Al2O3-La2O3 and SiO2-La2O3 were thermodynamically assessed and optimized with the substitution model. The obtained model parameters and Gibbs free energy of tine compounds and pure components were applied to the prediction of the liquidus surface and isothermal sections of Al2O3-SiO2-La2O3 | Lin LI and Zhujun TANG(Department of Materials Science & Engineering, Shanghai University, Shanghai 200072, China) To whom correspondence should be addressedE-mail: liling@public6.sta.net.cn Weiying SUN and Peiling WANG(Shanghai Institute of Ceramics, Chi | 1999 | Journal of Materials Science & Technology1999，15，5： | 3 |
| 9 | Impact of precipitation on the treatment of real ion-exchange brine using the H(2)- based membrane biofilm reactor显示文摘 | Van Ginkel SW Tang Y Rittmann BE | 2011 | Water Sci Technol2011，63，7： | 1 |
| 10 | Effect of hydrothennal tem- perature on the structure and electrochemical performance of manga- nese compound/Ordered mesoporous carbon composites for superca- pacitors 显示文摘 | Zhao Jiachang Tang Be Cao Jian | 2012 | Mater Manuf Processes2012，27，2： | 1 |
| 11 | Studies on the Coordination Polymer of Copper El ectropol yurushiol显示文摘The coordination polymer of copper electropolyurushiol(EPU-Cu2+) was obtained by the reaction of copper chloride with electropolyurushiol (EPU) in an isopropyl alcohol solution. The properties of EPU-Cu2+ were studied by means of ESR, FTIR, XPS, DMTA and TG-DTA.The results of AES show that the content of Cu2+ was 8.63%. The electric resistances of EPUCu2+ and EPU were determined to be 9.37×1010Ω and 7.90×1010Ω respectively. It was also ascertained that in EPU-Cu2+ each Cu2+ is coordinated with two units hydroxyl in EPU, making the EPU-Cu2+ cross-link further. As a result, the title polymer exhibits an insolubility in most of organic solutions, a higher glass transition temperature and thermal resistance. | TANG Jie-yuan~(**), ZHANG Wen-gong, SHEN Guo-cai and GAO Feng (Institute of Polymer SCience, Fujian Teachers University, Fuzhou 350007, P. R. China) To whom correspondence should be addressed. | 2001 | Chemical Research in Chinese Universities2001，17，3： | 1 |
| 12 | Imaging theory of nonlinear second harmonic and third harmonic generations in confocal microscopy显示文摘The imaging theory of nonlinear second harmonic generation (SHG) and third harmonic generation (THG) in confocal microscopy is presented in this paper. The nonlinear effect of SHG and THG on the imaging properties of confocal microscopy has been analyzed in detail by the imaging theory. It is proved that the imaging process of SHG and THG in confocal microscopy, which is different from conventional coherent imaging or incoherent imaging, can be divided into two different processes of coherent imaging. The three-dimensional point spread functions (3D-PSF) of SHG and THG confocal microscopy are derived based on the nonlinear principles of SHG and THG. The imaging properties of SHG and THG confocal microscopy are discussed in detail according to its 3D-PSF. It is shown that the resolution of SHG and THG confocal microscopy is higher than that of single- and two-photon confocal microscopy. | TANG Zhilie1, XING Da2 & LIU Songhao2 1. Department of Physics, South China Normal University, Guangzhou 510631, China 2. School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, China Correspondence should be addressed to Tang Zhilie (email: tangzhl@scnu.edu.cn) Received April 23, 2003 | 2004 | Science China(Physics,Mechanics & Astronomy)2004，47，1： | 1 |
| 13 | Sap flux:Up scaled canopy transpiration,stomatal conductance,and water use efficiency in an old growth forest in the Great Lakes region显示文摘 | Tang JW Bolstad PV Ewers BE | | 0，，： | 1 |
| 14 | Macrophages and glomerular crescent formation.Studies with rat nephrotoxic nephritis显示文摘 | Clarke BE Ham KN Tange JD | | 0，，： | 1 |
| 15 | Performance comparison between total variation (TV)-based compressed sensing and statistical iterative reconstruction algorithms显示文摘 | Tang Jie Nett BE Chen GH | 2009 | Phys Med Biol2009，54，： | 1 |
| 16 | Si nanowires grown from silicon oxide 显示文摘 | Wang N Tang Y H Zhang Y F Lee C S Be]lo I | 1999 | Chemical Phys- ics Letters1999，299，： | 1 |
| 17 | Synthesis, crystal structure and properties of binuclear manganese complex [(bipy)_2Mn_2(/J-O)(/i-Ac)_2(H_2O)_2](ClO_4)_2显示文摘Binuclear manganese complex [(bipy)2Mn2(μ-O)(μ-Ac)2(H2O)2](CIO4)2 was synthesized by the reaction of MnAc3 · 2H2O with 2,2’-bipyridine in the HAc-NaAc buffer (pH = 4.0). X-ray diffraction result for the single crystal shows that the crystal is monoclinic, space group C2/C, with a = 3.4082(7), 6 = 0.8644(2), c= 2.174 9(4) nm,β= 105.2°, V =6.186(2) nm3, Z= 8. There are two very strong peaks of UV-Vis spectrum in the range of 400-600 nm, which are similar to those of Mn catalase and Mn ribonuleotide reductase extracted from organisms. Cyclic voltammogram shows that the complex in CH3CN undergoes quasi-reversible one-electron reduction and oxidation at E1/2=1.15V. | LI Jun, YANG Shuming, ZHANG Fengxing, TANG Zongxun, SHI Qizhen & ZHOU Zhongyuan1. Department of Chemistry, Northwest University, Xi’an 710069, China 2. Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, China Correspondence should be addressed to Li Jun | 2000 | Chinese Science Bulletin2000，45，12： | 0 |
| 18 | Intense laser induced field ionization of C_(2)H_(2), C_(2)H_(4), and C_(2)H_(6)显示文摘Using HOMO Field Ionization Model, the tun-neling probabilities and the theoretical threshold intensities of the field ionizations of acetylene, ethylene, and ethane in intense laser field are calculated. C2H2, C2H4, and C2H6 were irradiated by 800 nm, 100 fs laser pulses with the intensity range of 1013—1014 W/cm2. A TOF-mass spectrometer was coupled to the laser system and used to experimentally in-vestigate the field ionization of these molecules. The experi-mental ionization threshold intensities are obtained. The calculating results of the three molecules agree well with the experimental results, indicating that HOMO Field Ionization Model is valid for the ionization of polyatomic molecules in intense laser field. | GAO Lirong1,2, JI Na1, XONG Yijia1, TANG Xiaoping1 & KONG Fan抋o1 1. Insistute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China 2. Graduate School of Chinese Academy Sciences, Beijing 100039, China Correspondence should be addressed to Kong Fan抋o (e-mail: kong@ mrdlab.icas.ac.cn) | 2003 | Chinese Science Bulletin2003，48，16： | 0 |
| 19 | Raman spectroscopy study of zinc finger ZNF191(243-368)显示文摘The structure of ZNF191(243-368), the zinc finger region protein of zinc finger protein ZNF191, and its structural change upon thermal and EDTA-induced denatu-ration were investigated by the Raman spectroscopy. It was demonstrated that the coordination between Zn2+ and His/Cys in ZNF191(243-368) is the essential factor to the stability of zinc finger, which plays an important role in maintaining the hydrophobic core and the secondary struc-ture in zinc finger, and the Raman spectroscopy is a powerful tool for investigating the structure of ZNF191(243-368). | HUANG Zhongxian, ZHANG Hailiang, YAO Wenhua, LIU Yuqi, ZHOU Jing & TANG Liming Chemical Biology Laboratory, Department of Chemistry, Fudan Univer-sity, Shanghai 200433, China Correspondence should be addressed to Huang Zhongxian (e-mail: zxhuang@fudan.edu.cn) | 2003 | Chinese Science Bulletin2003，48，16： | 0 |
| 20 | Adsorption behavior of hexadecyltrimethylammonium bromide (CTAB) to mica substrates as observed by atomic force microscopy显示文摘Atomic force microscopy is employed to study the adsorption behavior of CTAB to mica surfaces. Results show that conformational transitions from globular micelles→cylindrical micelles→flat films are observed in the process of the adsorption of CTAB to mica at twice the bulk critical concentration. However, in 0.5 cmc CTAB solution, the adsorbed CTAB molecules to mica form the layered film structures, and apparent conformational transitions do not appear. Meanwhile, the phenomena of the adsorption of CTAB to mica and the dissociation of mica sur- faces are periodically observed. | ZHAO Feng1,2, DU Yukou1, YANG Ping1, LI Xingchang2 & TANG Ji’an2 1. Chemistry and Chemical Engineering Department, Suzhou University, Suzhou 215006, China 2. Key Laboratory of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China Correspondence should be addressed to Tang Ji’an (email: jatang@iccas.ac.cn) | 2005 | Science China Chemistry2005，48，2： | 0 |
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