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| 1 | Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma显示文摘AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;P<0.001)and western blotting(1.92±0.26 vs 2.88±0.32;P<0.01).IHC revealed thatβ-actin expression is majorly distributed between epithelial and inflammatory cells of the tissues.Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal(2.46±0.13 vs 5.92±0.23,P<0.001),as well as,in tumor tissues(2.79±0.24 vs6.71±0.14,P<0.001).Further,comparison of immunostaining between normal and tumor tissues revealed that both epithelial and inflammatory cells overexpressβ-actin in tumor tissues,however,significant difference was observed only in inflammatory cells(5.92±0.23vs 6.71±0.14,P<0.01).Moreover,combined expression in epithelial and inflammatory cells also showed significant increase(4.19±0.15 vs 4.75±0.14,P<0.05)in tumor tissues.In addition,univariate analysis showed a positive correlation ofβ-actin level of inflammatory cells with tumor grade(P<0.05)while epithelial cells exhibited negative correlation(P>0.05).CONCLUSION:In gastric cancer,β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade. | Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta | 2014 | World Journal of Gastroenterology2014，20，34： | 2 |
| 2 | Review on thermal energy storage with phase change materials and applications 显示文摘 | Sharma A Tyagi V V Chen C R | 2009 | Renewable and Sustainable Energy Reviews2009，13，2： | 1 |
| 3 | Facile synthesis of ZrO2 powders:Control of morphology显示文摘 | Grover V Shukla R Tyagi A K | 2007 | Scr Mater2007，57，： | 1 |
| 4 | Detection of Vibrio parahaemolyticus in tropical shellfish by sybr green real-time PCR and evaluation of three enrichment media显示文摘 | TYAGI A SARAVANAN V KARUNASAGAR I | 2009 | International Journal of Food Microbiology2009，129，9： | 1 |
| 5 | SCS-CN-basedmodeling of sediment yield显示文摘 | Mishra S K Tyagi J V Singh V P | 2006 | Hydrol2006，324，14： | 1 |
| 6 | Skewing of the Thl/Th2 responses in mice due to variation in the level of expres- sion of an antigen in a recombinant BCG system显示文摘 | Dhar N Rao V Tyagi AK | 2003 | Immu- nol Lett2003，88，3： | 1 |
| 7 | A computed tomography-based analysis of proximal femoral geometry for lateral impingement with two types of proximal femoral nail anterotation in subtrchanteric fractures显示文摘 | Tyagi V Yang JH Oh KJ | | 0，，： | 1 |
| 8 | Review on ther- mal energy storage with phase change materials and applications 显示文摘 | SHARMA A TYAGI V V CHEN C R | 2009 | Renewable and Sustainable Energy Reviews2009，13，2： | 1 |
| 9 | pixel-based image forgery detection: A review 显示文摘 | Ansari MD Ghrera SP Tyagi V | 2014 | IETE Journal of Education2014，55，1： | 1 |
| 10 | Review on thermal energy storage with phase change materials and applications 显示文摘 | SHARMA A TYAGI V V CHEN C R | 2009 | Renewable and Sustainable Energy Reviews2009，13，： | 1 |
| 11 | SCS-CN-based Modeling of Sediment Yield 显示文摘 | Mishra S K Tyagi J V Singh V P | 2006 | Journal of Hydrology2006，324，： | 1 |
| 12 | Gender-based reciprocal expression of transforming growth factor-β1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis显示文摘 | Tyagi P Tyagi V Yoshimura N | 2009 | J Inflamm(Lond)2009，6，： | 1 |
| 13 | Sludge: A waste or renewable sourcefor energy and resources recovery-显示文摘 | Tyagi V K Lo S L | 2013 | Renewable andSustainable Energy Reviews2013，25，： | 1 |
| 14 | Energy and Exergy Analysis of Typical Renewable Energy Systems 显示文摘 | PARK S R PANDEY A K TYAGI V V | 2014 | Renewable and Sustainable Energy Reviews2014，30，2： | 1 |
| 15 | DevR-mediated adap-tive response in Mycobacterium tuberculosis H37Ra: links to as-paragine metabolism显示文摘 | Malhotra V Tyagi JS Clark-Curtiss JE | 2009 | Tuberculosis (Edinb)2009，89，2： | 1 |
| 16 | Spleen stiffness in patients with cirrhosis in predicting esophageal varices 显示文摘 | SHARMA P KIMAKE V TYAGI P | 2013 | Am J Gastroentero12013，108，7： | 1 |
| 17 | A computed tomography-based anal- ysis of proximal femoral genmetry for lateral impingement with two types of proximal femoral nail antirotation in subtrocimnteric frac- tures显示文摘 | Tyagi V Yang J H Oh K J | 2010 | Injury2010，41，8： | 1 |
| 18 | Developments in Organic Solid-liquid Phase Change Materials and Their Applications in Thermal Energy Storage显示文摘 | Sharma R K Ganesan P Tyagi V V | 2015 | Energy Conversion and Management2015，95，： | 1 |
| 19 | Thermal cycle testing of calcium chlo- ride hexahydrate as a possible PCM for latent heat storage 显示文摘 | Tyagi V V Buddhi D | 2008 | Solar Energy Mater Solar Cells2008，92，8： | 1 |
| 20 | Review on thermal energy storage with phase change materials and application显示文摘 | Sharma A Tyagi V V Chen C R | | 0，，02： | 1 |
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