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| 1 | Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma显示文摘AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;P<0.001)and western blotting(1.92±0.26 vs 2.88±0.32;P<0.01).IHC revealed thatβ-actin expression is majorly distributed between epithelial and inflammatory cells of the tissues.Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal(2.46±0.13 vs 5.92±0.23,P<0.001),as well as,in tumor tissues(2.79±0.24 vs6.71±0.14,P<0.001).Further,comparison of immunostaining between normal and tumor tissues revealed that both epithelial and inflammatory cells overexpressβ-actin in tumor tissues,however,significant difference was observed only in inflammatory cells(5.92±0.23vs 6.71±0.14,P<0.01).Moreover,combined expression in epithelial and inflammatory cells also showed significant increase(4.19±0.15 vs 4.75±0.14,P<0.05)in tumor tissues.In addition,univariate analysis showed a positive correlation ofβ-actin level of inflammatory cells with tumor grade(P<0.05)while epithelial cells exhibited negative correlation(P>0.05).CONCLUSION:In gastric cancer,β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade. | Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta | 2014 | World Journal of Gastroenterology2014，20，34： | 2 |
| 2 | Phenotypic instability and rapid gene silencing in newly formed Arabidopsis alloteraploids显示文摘 | Comai L Tyagi A P Winter K Holmes-Davis R Reynolds S H Stevens Y Byers B | 2000 | Plant Cell2000，12，9： | 2 |
| 3 | Assessment of the efficiency of various gene promoters via biolistics in leaf and regenerating seed callus of millets,Eleusine coracana and Echinochloa crusgalli显示文摘 | GUPTA P RAGHUVANSHI S TYAGI A K | 2001 | Plant Biotechnol2001，18，： | 1 |
| 4 | Negative thermal expansion in Er2W3O12 and Yb2W3O12 by high temperature X-ray diffraction显示文摘 | Sumithra S Tyagi A K Umarji A M | 2005 | Mater Sci Eng B2005，116，： | 1 |
| 5 | Review on thermal energy storage with phase change materials and applications 显示文摘 | Sharma A Tyagi V V Chen C R | 2009 | Renewable and Sustainable Energy Reviews2009，13，2： | 1 |
| 6 | Silibinin suppres- ses growth of human colorectal carcinoma SW480 cells in culture and xenograft through down-regulation of be- ta-catenin-dependent signaling 显示文摘 | Kaur M Velmurugan B Tyagi A | 2010 | Neoplasia2010，12，5： | 1 |
| 7 | Facile synthesis of ZrO2 powders:Control of morphology显示文摘 | Grover V Shukla R Tyagi A K | 2007 | Scr Mater2007，57，： | 1 |
| 8 | Solubility of Ce^4+ and Sr^2+ in the pyrochlore lattice of Gd2Zr2O7 for simulation of Pu and alkaline earth metal显示文摘 | PATWE S J TYAGI A K | 2006 | Ceram Intern2006，32，： | 1 |
| 9 | Recurrent and residual juvenile angiofibromas显示文摘 | TYAGI I SYAL R GOYAL A | 2007 | J Laryngol Otol2007，121，： | 1 |
| 10 | Detection of Vibrio parahaemolyticus in tropical shellfish by sybr green real-time PCR and evaluation of three enrichment media显示文摘 | TYAGI A SARAVANAN V KARUNASAGAR I | 2009 | International Journal of Food Microbiology2009，129，9： | 1 |
| 11 | Role of thoracic epidural block in improving post-operative outcome for septic patients:a preliminary report显示文摘 | TYAGI A SEELAN S SETHI AK | 2011 | Eur J Anaesthesiol2011，28，4： | 1 |
| 12 | Silibinin down-regulates survivin protein and mRNA expression and causes caspases activation and apoptosis in human bladder transitional-cell papilloma RT4 cells显示文摘 | TYAGI A K AGARWAL C SINGH R P | 2003 | Biochem Biophys Res Comm2003，312，4： | 1 |
| 13 | Role of thoracic cpidural block in improving post-operative outcome for septic patients: a preliminary report显示文摘 | Tyagi A Seelan S Sethi AK | 2011 | Eur J Anaesthesiol2011，28，4： | 1 |
| 14 | Mechanisms of endothelial dysfunction with development of type 1 diabetes mellitus role of insulin and C-peptide显示文摘 | Bratcher A P Rodriguez W E Tyagi S C | 2005 | Cell Biochem2005，96，6： | 1 |
| 15 | Antiproliferative and apoptotic effects of silibinin in rat prostate cancer cells 显示文摘 | Tyagi A Bhatia N Condon MS | 2002 | Prostate2002，53，3： | 1 |
| 16 | Powder characteristics and sinterability of ceria powders prepared through different routes显示文摘 | Purohit R D Saha S Tyagi A K | 2006 | Ceram Int2006，32，： | 1 |
| 17 | 显示文摘 | Kim H J Tyagi R K Fouda A E | 1996 | J Appl Polym Sci1996，62，： | 1 |
| 18 | Silibinin causes cellcycle arrest and apoptosis in human bladder transitional cellcarcinoma cells by regulating CDKI - CDK - cyclin cascade,and caspase 3 and PARP cleavages显示文摘 | Tyagi A Agarwal C Harrison G | 2004 | Carcinogenesis2004，25，9： | 1 |
| 19 | Review on ther- mal energy storage with phase change materials and applications 显示文摘 | SHARMA A TYAGI V V CHEN C R | 2009 | Renewable and Sustainable Energy Reviews2009，13，2： | 1 |
| 20 | Silibinin acti- vates p53-caspase 2 pathway and causes caspase-mediated cleavage of Cipl/p21 in apoptosis induction in bladder tran- sitional-cell papilloma RT4 cells: evidence for a regulatory loop between p53 and caspase 2显示文摘 | TYAGI A SINGH R P AGARWAL C | 2006 | Carcinogenesis2006，27，11： | 1 |
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