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| 1 | Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus显示文摘Objective:To develop diagnostic test for detection chikungunya virus(CHIKV and Dengue virus (DENV) infection.Methods:We have performed a rapid,accurate laboratory confirmative method to simultaneously detect,quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.Results:The assay’s sensitivity was 97.65%,specificity was 92.59% and accuracy was 95.82%when compared to conventional RT-PCR.Additionally,there was no cross-reaction between CHIKV,DENV,Japanese encephalitis virus,hepatitis C,hepatitis A or hepatitis E virus.Conclusions:This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction. | Piyathida Pongsiri Kesmanee Praianantathavorn Apiradee Theamboonlers Sunchai Payungporn Yong Poovorawan | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，5： | 3 |
| 2 | Molecular characterization and phylogenetic analysis of H1N1 and H3N2 human influenza A viruses among infants and children in Thailand显示文摘 | Salin C Thaweesak C Sunchai P | 2008 | Virus Res2008，132，： | 1 |
| 3 | Expression of TNF-α, TGF-β, IP-10 and IL-10 mRNA in kidneys of hamsters infected with pathogenic Leptospira显示文摘 | Alisa Lowanitchapat Sunchai Payungporn Amornpun Sereemaspun Pattama Ekpo Duangporn Phulsuksombati Yong Poovorawan Chintana Chirathaworn | 2009 | Comparative Immunology Microbiology and Infectious Diseases2009，，5： | 1 |
| 4 | Single step multiplex real-time RT-PCR for H5N1influenza A virus detection显示文摘 | Sunchai P Salin C Arunee C | 2006 | Journalof Virological Methods2006，131，2： | 1 |
| 5 | Genetic characterization of H5N1 influenza A viruses isolated from zoo tigers in Thailand显示文摘 | Alongkorn Amonsin Sunchai Payungporn Apiradee Theamboonlers Roongroje Thanawongnuwech Sanipa Suradhat Nuananong Pariyothorn Rachod Tantilertcharoen Sudarat Damrongwantanapokin Chantanee Buranathai Arunee Chaisingh Thaweesak Songserm Yong Poovorawan | 2005 | Virology2005，，2： | 1 |
| 6 | Single-Step Multiplex Reverse Transcription-Polymerase Chain Reaction (RT-PCR) for Influenza A Virus Subtype H5N1 Detection 显示文摘 | Sunchai P Piraya P Salin C | 2004 | Viral Immunol2004，17，4： | 1 |
| 7 | Genetic Characterization of H5N1 Influenza A Viruses Isolated from Zoo Tigers in Thailand显示文摘 | Alongkorn Amonsin Sunchai Payungporn Apiradee Theamboonlers | 2006 | Virology2006，314，2： | 1 |
| 8 | Single Step Multiplex real - time RT - PCR for H5N1 Influenza A Virus Detection 显示文摘 | Sunchai Payungporn Salin Chutinimitkul Arunee Chaisingh | 2006 | J Virol Methods2006，131，2： | 1 |
| 9 | Entire genome characteriza- tion of human papillomavirus type 16 from infecter Thai women with different cytological findings 显示文摘 | Woradee L Pairoj J Sunchai P | 2009 | Virus genes2009，39，1： | 1 |
| 10 | Early viral kinetics during hepatitis C virus genotype 6 treatment according to IL28B polymorphisms显示文摘AIM:To investigate the early viral kinetics and interleukin-28B(IL28B)polymorphisms of hepatitis C genotype6 during pegylated interferon and ribavirin therapy.METHODS:Sixty-five patients with chronic hepatitis C virus(HCV)infection treated with pegylated interferon and ribavirin(PEG-IFN/RBV)were included,of whom15(23.1%),16(24.6%)and 34(52.3%)patients were infected with hepatitis C genotype 1(HCV-1),genotype3(HCV-3)and genotype 6(HCV-6),respectively.Serum HCV-RNA levels were measured frequently during the first 4-wk of therapy.DNA extracted from samples was analyzed for the IL28B single nucleotide polymorphism(SNP)rs12979860 by polymerase chain reaction and direct sequencing.RESULTS:During the first 4-wk of therapy,the mean viral decline for patients with HCV-6(5.55±1.82 log10IU/mL)was comparable to that of patients with HCV-3(5.55±1.82 log10IU/mL vs 5.86±1.02 log10IU/mL,P=0.44)and was significantly higher than patients with HCV-1(5.55±1.82 log10IU/mL vs 4.23±1.99 log10IU/mL,P=0.04).In the HCV-6 group,the first phase(days 0-2)viral decline was significantly higher in patients with the favorable rs12979860 CC than non-CC genotypes(2.46±1.01 log10IU/mL/wk vs 1.70±0.67 log10IU/mL,respectively,P=0.045).A statistically insignificant decrease in the second-phase(days 7-28)decline was also found in patients with the CC genotype than those with the non-CC genotype,though not significantly different(1.24±0.64 log10IU/mL/wk vs 0.80±0.65 log10IU/mL/wk,respectively,P=0.172).At baseline,the SNP genotype was an independent predictor of rapid virological response but not of sustained virological response.CONCLUSION:The IL28B genotype was linked to an impact on early viral kinetics in response to PEG-IFN/RBV therapy in HCV-6 infected patients. | Srunthron Akkarathamrongsin Sunchai Payungporn Vo Duy Thong Kittiyod Poovorawan Phisit Prapunwattana Yong Poovorawan Pisit Tangkijvanich | 2014 | World Journal of Gastroenterology2014，20，30： | 0 |
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