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| 1 | One-year clinical study of NeuroR egen scaffold implantation following scar resection in complete chronic spinal cord injury patients显示文摘The objective of this clinical study was to assess the safety and feasibility of the collagen scaffold, Neuro Regen scaffold, one year after scar tissue resection and implantation. Scar tissue is a physical and chemical barrier that prevents neural regeneration. However, identification of scar tissue is still a major challenge. In this study, the nerve electrophysiology method was used to distinguish scar tissue from normal neural tissue, and then different lengths of scars ranging from 0.5–4.5 cm were surgically resected in five complete chronic spinal cord injury(SCI) patients. The NeuroR egen scaffold along with autologous bone marrow mononuclear cells(BMMCs), which have been proven to promote neural regeneration and SCI recovery in animal models, were transplanted into the gap in the spinal cord following scar tissue resection. No obvious adverse effects related to scar resection or Neuro Regen scaffold transplantation were observed immediately after surgery or at the 12-month follow-up. In addition, patients showed partially autonomic nervous function improvement, and the recovery of somatosensory evoked potentials(SSEP) from the lower limbs was also detected. The results indicate that scar resection and Neuro Regen scaffold transplantation could be a promising clinical approach to treating SCI. | Zhifeng Xiao Fengwu Tang Jiaguang Tang Huilin Yang Yannan Zhao Bing Chen Sufang Han Nuo Wang Xing Li Shixiang Cheng Guang Han Changyu Zhao Xiaoxiong Yang Yumei Chen Qin Shi Shuxun Hou Sai Zhang Jianwu Dai | 2016 | Science China(Life Sciences)2016，59，7： | 18 |
| 2 | Human placenta-derived mesenchymal stem cells loaded on linear ordered collagen scaffold improves functional recovery after completely transected spinal cord injury in canine显示文摘Traumatic spinal cord injury(SCI) is a major challenge in the clinic. In this study, we sought to examine the synergistic effects of linear ordered collagen scaffold(LOCS) and human placenta-derived mesenchymal stem cells(hPMSCs) when transplanted into completely transected beagle dogs. After 36 weeks observation, we found that LOCS+hPMSCs implants promoted better hindlimb locomotor recovery than was observed in the non-treatment(control) group and LOCS group. Histological analysis showed that the regenerated tissue after treatment was well integrated with the host tissue, and dramatically reduced the volume of cystic and chondroitin sulfate proteoglycans(CSPGs) expression. Furthermore, the LOCS+hPMSCs group also showed more neuron-specific βIII-tubulin(Tuj-1)-and NeuN-positive neurons in the lesion area, as well as axonal regeneration, remyelination and synapse formation in the lesion site. Additionally, dogs in the LOCS+hPMSCs group experienced enhanced sprouting of both ascending(CGRP-positive) sensory fibers and descending(5-HT-and TH-positive) motor fibers at the lesion area. All these data together suggested that the combined treatment had beneficial effects on neuronal regeneration and functional improvement in a canine complete transection model. Therefore, LOCS+hPMSCs implantation holds a great promise for bridging the nerve defect and may be clinically useful in the near future. | Sufang Han Zhifeng Xiao Xing Li Huan Zhao Bin Wang Zhixue Qiu Zhi Li Xin Mei Bai Xu Caixia Fan Bing Chen Jin Han Yanzheng Gu Huilin Yang Qin Shi Jianwu Dai | 2018 | Science China(Life Sciences)2018，61，1： | 15 |
| 3 | Properties of Supersulphated Phosphogysum-slag Cement (SSC) Concrete显示文摘Supersulphated phosphogysum-slag cement(SSC) is a newly developed non-burned cementitious material mainly composed of phosphogysum(PG) and ground granulated blast furnace slag(GGBFS), with small amount of steel slag(SS) and clinker(CL). SSC is a kind of environmentally-friendly cementitious material due to its energy-saving, low-carbon emission, and waste-utilization. We prepared concretes with supersulphated phosphogysum-slag cement, and studied the mechanical properties, microproperties and resistance to chloride penetration of concrete in comparison with those of portland slag cement(PSC) and ordinary portland cement(OPC) concrete. The test results show that the compressive strength of SSC concrete can reach 38.6 MPa after 28 d, close to PSC concrete and OPC concrete. Microanalyses indicate that large quantities of ettringite and C-S-H, and little amount of Ca(OH)2 are generated during the hydration of SSC. The dense cement paste structure of SSC is formed by ettringite and C-S-H, surrounded unreacted phosphogysum. The property of resistance to chloride penetration of SSC concrete is better than PSC and OPC concrete due to the fact that SSC can form much more ettringite to solidify more Cl-. | 丁沙 水中和 CHEN Wei LU Jianxin TIAN Sufang | 2014 | Journal of Wuhan University of Technology(Materials Science)2014，29，1： | 6 |
| 4 | The Three-Dimensional Collagen Scaffold Improves the Sternness of Rat Bone Marrow Mesenchymal Stem Cells显示文摘因为他们的自强和 multipotential 能力,间充质的干细胞(MSC ) 为许多疾病的治疗显示出大诺言。MSC 通常是有教养的在上二维(2D ) 在 vitro 的底层。有指示,他们可以同时失去他们是的 stemness 和 multipotentiality 延长 2D 的结果文化。在这研究,我们使用了三维(3D ) 是的骨胶原脚手架老鼠 MSC 搬运人并且在 3D 骨胶原脚手架上把 MSC 的性质与单层作比较有教养的 MSC。结果证明骨胶原脚手架对老鼠 MSC 坚持和增长合适。与在 2D 文化下面的 MSC 相比,更重要地, 3D MSC 显著地维持了 stemness 基因(Oct4, Sox2, Rex-1 和 Nanog ) 的更高的表示层次,产出形成殖民地的 units-fibroblastic (CFU-F ) 的高频率并且显示出在正式就职之上的提高的 osteogenic 和 adipogenic 区别效率。因此, 3D 骨胶原脚手架可能为当在 vitro 维持干细胞性质时,扩展老鼠 MSC 是有益的。 | Sufang Han Yannan Zhao Zhifeng Xiao Jin Han Bing Chen Lei Chen Jianwu Dai | 2012 | Journal of Genetics and Genomics2012，39，12： | 6 |
| 5 | The Arabidopsis phosphatase PP2C49 negatively regulates salt tolerance through inhibition of At HKT1;1显示文摘Type 2C protein phosphatases(PP2Cs)are the largest protein phosphatase family.PP2Cs dephosphorylate substrates for signaling in Arabidopsis,but the functions of most PP2 Cs remain unknown.Here,we characterized PP2 C49(AT3G62260,a Group G PP2C),which regulates Na^(+)distribution under salt stress and is localized to the cytoplasm and nucleus.PP2C49 was highly expressed in root vascular tissues and its disruption enhanced plant tolerance to salt stress.Compared with wild type,the pp2c49 mutant contained more Na^(+)in roots but less Na^(+)in shoots and xylem sap,suggesting that PP2C49 regulates shoot Na^(+)extrusion.Reciprocal grafting revealed a root-based mechanism underlying the salt tolerance of pp2 c49.Systemic Na+distribution largely depends on AtHKT1;1 and loss of function of AtHKT1;1 in the pp2c49 background overrode the salt tolerance of pp2c49,resulting in salt sensitivity.Furthermore,compared with plants overexpressing PP2C49 in the wild-type background,plants overexpressing PP2C49 in the athtk1;1 mutant background were sensitive to salt,like the athtk1;1 mutants.Moreover,protein-protein interaction and two-voltage clamping assays demonstrated that PP2C49 physically interacts with AtHKT1;1 and inhibits the Na^(+)permeability of AtHKT1;1.This study reveals that PP2C49 negatively regulates AtHKT1;1 activity and thus determines systemic Na^(+)allocation during salt stress. | Moli Chu Pengwang Chen Sufang Meng Peng Xu Wenzhi Lan | 2021 | Journal of Integrative Plant Biology2021，63，3： | 5 |
| 6 | Simultaneous determination of 15 pesticide residues in Chinese cabbage and cucumber by liquid chromatography-tandem mass spectrometry utilizing online turbulent flow chromatography显示文摘In this experiment,a liquid chromatography tandem mass spectrometry method was built to determine 15 pesticide residues in Chinese cabbage and cucumber samples based on online turbulent flow chromatography purification.After modified quick,easy,cheap,effective,rugged,and safe(QuEChERS)extraction,extracts were directly injected to the TLX(TurboFlow Liquid Xcalibur)system and brought to TurboFlow™columns for on-line purification and then transferred to analytical column for further separation and analysis.TurboFlow™columns types,transfer flow rate,and transfer time were optimized.Limits of detection and limits of quantification of the method obtained for 15 pesticide residues were ranged between 0.2–1.0μg/kg and 0.5–2.0μg/kg in Chinese cabbage and cucumber samples.Recoveries of pesticide residues were in range of 75.3%–103.7%.Matrix effects for 15 pesticides were in range of 5.6%–106.6%.The developed method has been successfully used for the determination of 15 pesticide residues in real samples. | Sufang Fan Junmei Ma Meirong Cao Juan Wang Leilei Zhang Yan Zhang Qiang Li Jia Chen | 2021 | Food Science and Human Wellness2021，10，1： | 5 |
| 7 | A preliminary investigation on genetic diversity of Sousa chinensis in the Pearl River Estuary and Xiamen of Chinese waters显示文摘In this study, the mitochondrial DNA (mtDNA) control region and the mitochondrial cytochrome b gene of stranded Indo-Pacific humpback dolphin (Sousa chinensis) samples from the Pearl River Estuary and Xiamen waters were sequenced and analyzed. The result of mtDNA control region revealed 34 variable sites and four unique haplotypes (named as A, B, C and D) identified among the total samples from these two water areas, and the most common haplotype (A) was shared by 75% of the dolphins sampled from the two water areas. The haplotypic diversity (h) was 0.455 and the nucleotide diversity (π) was 0.0088. The phylogenetic analysis showed that the hap- lotype A, C, and D were closely related, but the haplotype B (unique for XM01 from Xiamen) was far from the other three. By scanning cytochrome b fragments, two haplotypes (A and B) were identified in these two water areas, and the most common haplotype (A) was shared by 91.67% individuals, while XM01 from Xiamen as the only exception. The data suggest that there is a possibility of gene exchange between the two populations in the Pearl River Estuary and Xiamen waters, and there possibly exists a unique maternal lineage in Xiamen waters. | Hongshan Chen Kan Zhai Jialin Chen Yulong Chen Haijun Wen Sufang Chen Yuping Wu | 2008 | Journal of Genetics and Genomics2008，35，8： | 4 |
| 8 | Antrodia cinnamomea exerts an anti-hepatoma effect by targeting PI3K/AKT-mediated cell cycle progression in vitro and in vivo显示文摘Antrodia cinnamomea is extensively used as a traditional medicine to prevention and treatment of liver cancer.However,its comprehensive chemical fingerprint is uncertain,and the mechanisms,especially the potential therapeutic target for anti-hepatocellular carcinoma(HCC)are still unclear.Using UPLC-Q-TOF/MS,139 chemical components were identified in A.cinnamomea dropping pills(ACDPs).Based on these chemical components,network pharmacology demonstrated that the targets of active components were significantly enriched in the pathways in cancer,which were closely related with cell proliferation regulation.Next,HCC data was downloaded from Gene Expression Omnibus database(GEO).The Cancer Genome Atlas(TCGA)and Dis Ge NET were analyzed by bioinformatics,and 79 biomarkers were obtained.Furtherly,nine targets of ACDP active components were revealed,and they were significantly enriched in PI3 K/AKT and cell cycle signaling pathways.The affinity between these targets and their corresponding active ingredients was predicted by molecular docking.Finally,in vivo and in vitro experiments showed that ACDPs could reduce the activity of PI3 K/AKT signaling pathway and downregulate the expression of cell cycle-related proteins,contributing to the decreased growth of liver cancer.Altogether,PI3 K/AKT-cell cycle appears as the significant central node in anti-liver cancer of A.Cinnamomea. | Yan Zhang Pin Lv Junmei Ma Ning Chen Huishan Guo Yan Chen Xiaoruo Gan Rong Wang Xuqiang Liu Sufang Fan Bin Cong Wenyi Kang | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 2 |
| 9 | A photosensitive fluorinated ionic complex with tunable surface wetting properties : Mesostructure and photosensitivity显示文摘 | Lu Xuemin Xiao Sufang Chen Xumeng | 2011 | Polymer Chemistry2011，2，11： | 1 |
| 10 | 显示文摘 | WANG Zonghua LUO Guoan CHEN Jianfang XIAO Sufang WANG Yining | 2003 | Electrophoresis2003，24，24： | 1 |
| 11 | EBV encoded miR-BHRF1-1 potentiates viral lytic replication by downregulating host p53 in nasopharyngeal carcinoma显示文摘 | Zijian Li Xue Chen Lili Li Sufang Liu Lifang Yang Xiaoqian Ma Min Tang Ann M. Bode Zigang Dong Lunquan Sun Ya Cao | 2011 | International Journal of Biochemistry and Cell Biology2011，，2： | 1 |
| 12 | β-Cyclodextrin Incorporated Carbon Nanotubes-Modified Electrodes for Simultaneous Determination of Adenine and Guanine显示文摘 | Wang Zonghua Xiao Sufang Chen Yue | 2006 | Journal of Electroanalytical Chemistry2006，589，2： | 1 |
| 13 | In vitro Transcriptome Analysis of Two Chinese Isolates of Streptococcus suis Serotype 2显示文摘The Streptococcus suis serotype 2(S. suis 2) isolates 05ZYH33 and 98HAH33 have caused severe human infections in China. Using a strand-specific RNA-seq analysis, we compared the in vitro transcriptomes of these two Chinese isolates with that of a reference strain(P1/7). In the89 K genomic island that is specific to these Chinese isolates, a toxin–antitoxin system showed relatively high levels of transcription among the S. suis. The known virulence factors with high transcriptional activity in these two highly-pathogenic strains are mainly involved in adhesion, biofilm formation, hemolysis and the synthesis and transport of the outer membrane protein. Furthermore,our analysis of novel transcripts identified over 50 protein-coding genes with one of them encoding a toxin protein. We also predicted over 30 small RNAs(s RNAs) in each strain, and most of them are involved in riboswitches. We found that six s RNA candidates that are related to bacterial virulence, including csp A and rli38, are specific to Chinese isolates. These results provide insight into the factors responsible for the difference in virulence among the different S. suis 2 isolates. | Dake Zhang Nan Du Sufang Ma Qingtao Hu Guangwen Lu Wei Chen Changqing Zeng | 2014 | Genomics, Proteomics & Bioinformatics2014，12，6： | 1 |
| 14 | Novel Aqueous Soluble Cobalt Phthalocyanine : Synthesis and Catalytic Activ- ity on Oxidation of 2-Mercaptoethanol显示文摘 | Pan Yong Chen Wenxing Lu Sufang | 2005 | Dyes Pigments''2005，66，2： | 1 |
| 15 | β-cyclodextrin incorporated carbon nanotubes-modified electrodes for simultaneous determination of adenine and guanine显示文摘 | Wang Zonghua Xiao Sufang Chen Yue | 2006 | Journal of Electroanalytical Chemistry2006，，589： | 1 |
| 16 | Preparation and properties of phosphorous–nitrogen containing UV-curable polymeric coatings based on thiol–ene click reaction显示文摘 | Chunhai Liu Tingcheng Li Junheng Zhang Sufang Chen Zhicai Xu Aiqing Zhang Daohong Zhang | 2016 | Progress in Organic Coatings2016，，： | 1 |
| 17 | Effect of preparation method on halloysite supported cobalt catalysts for Fischer-Tropsch synthesis显示文摘Novel cobalt Fischer-Tropsch synthesis(FTS) catalysts were prepared from natural halloysite nanotubes(HNT) by double-solvent and wetimpregnation methods,and characterized by TEM,XRD,TPR and N2 adsorption-desorption.Comparing with the catalyst prepared by wetimpregnation method,the catalyst prepared by double-solvent method reduces Co3O4 particle migration and agglomeration due to size-induced effect,thus showing higher catalytic activity for Fischer-Tropsch synthesis. | Sufang Chen Jinlin Li Yuhua Zhang Daohong Zhang Junjiang Zhu | 2012 | Journal of Natural Gas Chemistry2012，21，4： | 0 |
| 18 | Five Large Generation Groups: Competing in Capital Operation显示文摘Since the reform of electric power industry in 2002, the newly established five large generation groups have been persisting in the development strategy of 'taking electricity as the core and extending to up-and-downstream businesses.' Stringent measures were taken in capital operation and their potential has been shown through electric power as- sets acquiring, coal and financial resources investing, capital market financing as well as power utility restructuring. The five groups are playing more and more important roles in merger and acquisition (M&A) and capital markets. | Chen Zongfa and Yin Hongjun China Huadian Corporation Zhang Sufang | 2008 | Electricity2008，19，3： | 0 |
| 19 | Initial stiffness and moment resistance of reinforced joint with end-plate connection显示文摘In beam-to-column joint with bolted end-plate connection,the structural details of column flange reinforced by backing plate and column web panel reinforced by supplementary plate are analyzed.The joint is divided into some basic components,and the initial stiffness of each component is obtained.Especially,the initial stiffness of reinforced components is drawn by theoretical model and finite element analysis.The initial stiffness of reinforced joint can be obtained by assembling the initial stiffness of each component.The design moment resistance of column flange reinforced by backing plate is deduced based on yield line method,and the design moment resistances of other components are deduced based on present codes.The design moment resistance of the reinforced joint is then determined by the minimum of the design moment resistances of all components.By comparison with the results of finite element calculation,it is verified that the method to calculate the initial stiffness of reinforced joint is accurate enough to be used to estimate the rigid behavior of the joint and to make parametric study. | Sufang WANG Yiyi CHEN | 2009 | Frontiers of Structural and Civil Engineering2009，3，4： | 0 |
| 20 | Rapid-acting antidepressants targeting modulation of the glutamatergic system: clinical and preclinical evidence and mechanisms显示文摘Major depressive disorder(MDD)is a devastating mental illness that affects approximately 20%of the world's population.It is a major disease that leads to disability and suicide,causing a severe burden among communities.Currently available medications for treating MDD target the monoaminergic systems.The most prescribed medications include selective serotonin reuptake inhibitors and selective norepinephrine reuptake inhibitors.However,these medications have serious drawbacks,such as a delayed onset requiring weeks ormonths to reach efficacy and drug resistance,as one-third of patients are unresponsive to the medications.Therefore,it is imperative to develop novel therapies with rapid action,high efficacy and few adverse effects.The discovery of the rapid antidepressant effect of ketamine has triggered tremendous enthusiasm for studying new antidepressants that target the glutamatergic system in the central nervous system.Many agents that directly or indirectly modulate the glutamatergic system have been shown to provide rapid and lasting antidepressant action.Among these agents,ketamine,an antagonist of metabotropic glutamate 2/3 receptors,and scopolamine,an unspecific muscarinic acetylcholine receptor antagonist,have been extensively studied.In this review,we discuss the clinical and preclinical evidence supporting theantidepressant efficacy of these agents and the current understanding of the underlying mechanisms. | Shikai Wang Sufang Tang Jintao Huang Huanxin Chen | 2022 | General Psychiatry2022，35，6： | 0 |
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