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您的检索式：作者名="Stephen R.Williams"
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| 1 | Reference quality assembly of the 3.5-Gb genome of Capsicum annuum from a single linked-read library显示文摘Linked-Read sequencing technology has recently been employed successfully for de novo assembly of human genomes,however,the utility of this technology for complex plant genomes is unproven.We evaluated the technology for this purpose by sequencing the 3.5-gigabase(Gb)diploid pepper(Capsicum annuum)genome with a single Linked-Read library.Plant genomes,including pepper,are characterized by long,highly similar repetitive sequences.Accordingly,significant effort is used to ensure that the sequenced plant is highly homozygous and the resulting assembly is a haploid consensus.With a phased assembly approach,we targeted a heterozygous F1 derived from a wide cross to assess the ability to derive both haplotypes and characterize a pungency gene with a large insertion/deletion.The Supernova software generated a highly ordered,more contiguous sequence assembly than all currently available C.annuum reference genomes.Over 83%of the final assembly was anchored and oriented using four publicly available de novo linkage maps.A comparison of the annotation of conserved eukaryotic genes indicated the completeness of assembly.The validity of the phased assembly is further demonstrated with the complete recovery of both 2.5-Kb insertion/deletion haplotypes of the PUN1 locus in the F1 sample that represents pungent and nonpungent peppers,as well as nearly full recovery of the BUSCO2 gene set within each of the two haplotypes.The most contiguous pepper genome assembly to date has been generated which demonstrates that Linked-Read library technology provides a tool to de novo assemble complex highly repetitive heterozygous plant genomes.This technology can provide an opportunity to cost-effectively develop high-quality genome assemblies for other complex plants and compare structural and gene differences through accurate haplotype reconstruction. | Amanda M.Hulse-Kemp Shamoni Maheshwari Kevin Stoffel Theresa A.Hill David Jaffe Stephen R.Williams Neil Weisenfeld Srividya Ramakrishnan Vijay Kumar Preyas Shah Michael C.Schatz Deanna M.Church Allen Van Deynze | 2018 | Horticulture Research2018，5，1： | 4 |
| 2 | Theoretical Analysis of the Fluctuation Theorem Applied to Electric Circuits显示文摘We carry out a theoretical analysis of the fluctuations in the Johnson–Nyquist noise in an RC electric circuit being driven by a non-ideal constant current source. Using the appropriate Langevin equation, we derive the fluctuation theorem for the system. This analysis exhibits some interesting features. Firstly, the average of the dissipation function is not necessarily just an instantaneous entropy production, i.e. the work being done on the circuit by the external power source divided by the ambient temperature. Secondly, by appropriate selection of the values for the circuit components,the transient response of the system can be made identical to the nonequilibrium steady state response. In the limit of an ideal current source, the average of the dissipation function reduces to the entropy production. Our analysis accurately reproduces published experimental results. | Charlotte F.Petersen Elmars Krausz Denis J.Evans Stephen R.Williams | 2014 | Communications in Theoretical Physics2014，61，10： | 0 |
| 3 | 一体化物流系统对电子商务和企业资源规划系统的影响显示文摘供应链观念的发展要求物流组织内外并重地改善信息的流动.在许多公司里,信息量增长的需求促进了物流信息系统(LIS)和供应链信息系统的一体化进程.在不久的将来,电子商务、企业资源规划和其他物流信息系统的工具和技术愈来愈多的应用将会决定商业流程的发展方向.公司在做出决定支持自己的供应链系统时应该明白自己的选择及其带来的影响. | Stephen M.Rutner Brian J.Gibson Susan R.Williams 何立杨 陈华殊 | 2004 | 经济资料译丛2004，，2： | 0 |
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