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| 1 | Claudin-11 and occludin are major contributors to Sertoli cell tight junction function, in vitro显示文摘Sertoli 房间紧密的连接(TJ ) 是血睾丸障碍的关键部件,在它扣押开发在生精的小管以内经历精子发生的细菌房间的地方。神经质地调整的 claudin-11 是在不孕涉及障碍功能和它的鼠科的猛烈结果的批评 transmembrane 蛋白质。我们试图估计份量上到 TJ 功能的 claudin-11 的贡献的意义在 vitro,使用调停 siRNA 的基因 silencing。我们也进行了 occludin 的贡献的分析, TJ 的另一内在的 transmembrane 蛋白质。claudin-11 或 occludin 的 Silencing 在一个不成熟的老鼠 Sertoli 房间文化模型用 siRNA 被进行。Transepithelial 电的抵抗被用来在整个文化估计份量上 TJ 功能。在 siRNA 处理以后的二天,房间为对 mRNA 的 RT-PCR 评价为连接蛋白质或 lyzed 的 immunocytochemical 本地化被修理表示。claudin-11, occludin,或两个的 Silencing 在 55% 的 TJ 功能导致了重要减少(P <0.01 ) , 51%(P <0.01 ) ,并且 62%(P <0.01 ) 分别地。数据在到 Sertoli 房间 TJ 的指向的蛋白质的本地化是有在 mRNA 表示和显著减小的重要减少的伴随物。我们提供 claudin-11 显著地贡献的量的证据(P <0.01 ) 到 Sertoli 房间 TJ 在 vitro 工作。有趣地, occludin,神经质地在不孕被调整然而并非含有直到迟了的成人生活,也是一重要(P <0.01 ) 贡献者到障碍功能。我们的数据与一致在清楚地为这些表明一个角色的 vivo 研究,在维持正常 TJ 障碍的蛋白质组织并且工作。 | Mark J McCabe Caroline FH Foo Marcel E Dinger Peter M Smooker Peter G Stanton | 2016 | Asian Journal of Andrology2016，18，4： | 7 |
| 2 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 3 | The Gut Microbiota and the Liver Pathophysiological and Clinical Implications显示文摘 | Eamonn M .M. Quigley Catherine Stanton Eileen F. Murphy | 2012 | Journal of Hepatology2012，，： | 2 |
| 4 | Activation of the hexosamine pathway causes oxidative stress and abnormal embryo gene expressicn:involvement in diabetic teratogenesis显示文摘 | Horal M Zhang Z Stanton R | 2004 | Birth Defects Res A Clin Mol Teratol2004，70，8： | 1 |
| 5 | Emergy evaluation of the performance and sustainability of three agricultural systems with different scales and management显示文摘 | Martin J F Diemont S A W Powell E Stanton M Levy-Tacher S | 2006 | Agriculture Ecosystems&Environment2006，115，14： | 1 |
| 6 | Test for the presence of sulfate-reducing bacteria显示文摘 | TATNALL R E STANTON K M EBERSOLE R C | 1988 | General interest1988，27，8： | 1 |
| 7 | Procedural interference in perceptual classification:Implicit learning or cognitive complexity?显示文摘 | Nosofsky R M Stanton R D Zaki S R | 2005 | Memory & Cognition2005，33，7： | 1 |
| 8 | Negative Affect and Job Search:Further Examination of the Reverse Causation Hypothesis 显示文摘 | CROSSLEY C D STANTON J M | 2005 | Journal of Vocational Behavior2005，66，4： | 1 |
| 9 | Simple phenomenological model for reinforcing steel under arbitrary load 显示文摘 | Hoehler M S Stanton J F | 2006 | Journal of Structural Engineering2006，132，7： | 1 |
| 10 | Changes in vegetation and soil fertility along a predictable snowmelt gradient in the Mosquito Range,Colorado,USA显示文摘 | Stanton M L Rejmnek M GalenC | 1994 | Arct Alp Res1994，26，： | 1 |
| 11 | Seed variation in wild radish: effect of seed size on components of seedling and adult fitness 显示文摘 | Stanton M L | 1984 | Ecology1984，65，： | 1 |
| 12 | Conjugated linoleic acid in bovine milk fat:a food -based approach to cancer chemoprevention 显示文摘 | M OShea F Lawless C Stanton R Devery | 1998 | Trends in Food Science & Technology1998，，9： | 1 |
| 13 | Responses of snow bed plant species to changes in growing season length显示文摘 | Galen C Stanton M L | 1995 | Ecology1995，74，： | 1 |
| 14 | Eyeblink classical conditioning in the preweanling lamb显示文摘 | Johnson TB Stanton M E Goodlett C R | 2008 | BehavNeurosci2008，122，3： | 1 |
| 15 | 显示文摘 | McCauley TG Hamaguchi N Stanton M | 2003 | Anal Biochem2003，319，2： | 1 |
| 16 | Broadband acoustic baekscatter and high-resolution morphology of fish: measurement and modeling显示文摘 | REEDER D B JECH J M STANTON T K | 2004 | Journal of the Acoustical Society of America2004，116，2： | 1 |
| 17 | The eft ect of chronic low back pain on size and contraction of the lumbar multifi dus muscle 显示文摘 | Wallwork TL Stanton WR Freke M | 2009 | Man Ther2009，14，5： | 1 |
| 18 | 显示文摘 | Hamaguchi N Ellington A Stanton M | 2001 | Anal Biochem2001，294，2： | 1 |
| 19 | Non-invasive assessment lymph edematous limb 显示文摘 | STANTON A M BADGER C SITZIA | 2000 | J Lymphol2000，33，3： | 1 |
| 20 | Nonenzymatic browning in kiwifruit juice concertrate systems during storage显示文摘 | Wong M Stanton D W | 1998 | J of Food Sci1998，，3： | 1 |
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