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| 1 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 2 | The underground in grassland显示文摘 | Stanton N L | 1988 | Annual Review of Ecology and Systematics1988，19，： | 1 |
| 3 | HIV/AIDS-related sex- ual risk behaviors among rural residents in China Potential role of rural -to-urban migration显示文摘 | Li X Zhang L Stanton B Fang X | 2007 | AIDS Education and Prevention2007，19，5： | 1 |
| 4 | Changes in vegetation and soil fertility along a predictable snowmelt gradient in the Mosquito Range,Colorado,USA显示文摘 | Stanton M L Rejmnek M GalenC | 1994 | Arct Alp Res1994，26，： | 1 |
| 5 | Seed variation in wild radish: effect of seed size on components of seedling and adult fitness 显示文摘 | Stanton M L | 1984 | Ecology1984，65，： | 1 |
| 6 | Coping Resources, Coping Processes, and Mental Health显示文摘 | Taylor S E Stanton A L | 2007 | Annual Review of Clini- cal Psychology2007，3，1： | 1 |
| 7 | Superiority of ambulatory over clinic blood pressure measurement in predicting mortality: the Dublin outcome study显示文摘 | Dolan E Stanton A Thijs L | 2005 | Hypertension2005，46，1： | 1 |
| 8 | Responses of snow bed plant species to changes in growing season length显示文摘 | Galen C Stanton M L | 1995 | Ecology1995，74，： | 1 |
| 9 | The standardization of terminology of lower u-rinary tract function recommended by the international continence society显示文摘 | Abrams P Blaivas J G Stanton S L | 1990 | Int Urogy-necol1990，1，： | 1 |
| 10 | Auditory evoked potentials and auditory behavior following prenatal and perinatal asphyxia in rhesus monkeys显示文摘 | Mirsky AF Orren MM Stanton L | 1979 | Devv Psychobiol1979，12，4： | 1 |
| 11 | Determination of seasonality in southern hairy-nosed wombats(Lasiorhinus latifrons)by analysis of fecal androgens 显示文摘 | Hamihon R A Stanton P G Donnell L | 2000 | Biology of Repro-duction2000，63，： | 1 |
| 12 | Superiority of ambulatory over clinic blood pressure measurement in predicting mortality:the Dublin outcome study显示文摘 | Dolan E Stanton A Thijs L | 2005 | Hypertension2005，46，： | 1 |
| 13 | Perceptions of Shigella and of Shigella vaccine among rural Chinese:compatibility with Western models of behavioral change显示文摘 | YOULONG G STANTON BF VON SEIDLEN L | 2004 | Southeast Asian J Trop Med Public Health2004，35，： | 1 |
| 14 | Superiority of ambulatory over clinic blood pressuremeasurement in predictingmortality: the Dublin outcome study显示文摘 | Dolan E Stanton A Thijs L | 2005 | Hypertension2005，46，1： | 1 |
| 15 | Superiority of ambulatory over clinic blood pressure measurement in predicting mortality~ the Dublin outcome study显示文摘 | Dolan E Stanton A Thijs L | 2005 | Hypertension2005，46，1： | 1 |
| 16 | Superiority of ambulatory over clinic blood pressure measurement in predicting mortali- ty : the Dublin outcome study 显示文摘 | DolanE Stanton A Thijs L | 2005 | Hypertension2005，46，1： | 1 |
| 17 | Emotionally expressive coping predicts psychological and physical adjustment to breast cancer显示文摘 | Stanton AL Danoff BS Cameron C L | 2000 | J Consult Clin Psychol2000，68，5： | 1 |
| 18 | Fast,cost-effective development of species-specific microsatellitemarkers by genomic sequencing显示文摘 | Abdelkrim J Robertson B C Stanton J A L | 2009 | Biotechniques2009，46，3： | 1 |
| 19 | p38 MAP kinase signaling is necessary for rat chondrosarcoma cell proliferation 显示文摘 | Halawani D Mondeh R Stanton L A | 2004 | Oncogene2004，23，20： | 1 |
| 20 | Quality of life at the end of primary treatment of breast cancer:first results from moving beyond cancer randomized trial显示文摘 | Ganz P A Kwan L Stanton A L | 2003 | J Natl Cancer Inst2003，96，5： | 1 |
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