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| 1 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 2 | Systemic chemotherapy with or without cetuximab in patients with resectable colorectal liver metastasis: the New EPOC randomised controlled trial显示文摘 | John Primrose Stephen Falk Meg Finch-Jones Juan Valle Derek O’Reilly Ajith Siriwardena Joanne Hornbuckle Mark Peterson Myrddin Rees Tim Iveson Tamas Hickish Rachel Butler Louise Stanton Elizabeth Dixon Louisa Little Megan Bowers Sian Pugh O James Garden D | 2014 | Lancet Oncology2014，，6： | 2 |
| 3 | An overview of the PRESSS five-story precast test building 显示文摘 | Nakaki S D Stanton J F and Sritharan S | 1999 | PCI Journal1999，44，2： | 1 |
| 4 | How to avoid iat-mgenic morbidity and mortality increasing availability of oxytocin and mispmstol for PPH prevention? 显示文摘 | Lovold A Stanton C Armbuser D et d | 2008 | Int J Gy- naecol Obstet2008，103，3： | 1 |
| 5 | Broadband acoustic baekscatter and high-resolution morphology of fish: Mea- surement and modeling显示文摘 | REEDER D JECH J STANTON T | 2004 | Journal of the Acoustical So- ciety of America2004，116，2： | 1 |
| 6 | Procedural interference in perceptual classification:Implicit learning or cognitive complexity?显示文摘 | Nosofsky R M Stanton R D Zaki S R | 2005 | Memory & Cognition2005，33，7： | 1 |
| 7 | Negative Affect and Job Search:Further Examination of the Reverse Causation Hypothesis 显示文摘 | CROSSLEY C D STANTON J M | 2005 | Journal of Vocational Behavior2005，66，4： | 1 |
| 8 | How to avoid iatrogenic morbidity and mortality while increasing availability of oxytocin and misoprostol for PPH prevention 显示文摘 | Lovold A Stanton C Armbruster D | 2008 | Int J Gynaecol Obstet2008，103，3： | 1 |
| 9 | Posterior colporrhaphy is superior to the transanal repair for treatment of posterior vaginal wall prolapse显示文摘 | Kahn MA Stanton SL Kumar D | 1999 | Neurourol Urodyn1999，18，： | 1 |
| 10 | Broadband acoustic baekscatter and high-resolution morphology of fish: measurement and modeling显示文摘 | REEDER D B JECH J M STANTON T K | 2004 | Journal of the Acoustical Society of America2004，116，2： | 1 |
| 11 | Frequency Modulation and Wavelength Modulation Spectroscopies显示文摘 | BOMSE S D STANTON C A SILVER A J | 1992 | Applied Optics1992，31，6： | 1 |
| 12 | Preoperative transcatheter closptal defects of congenital muscular ventricular septal defects 显示文摘 | BRIDGES NANCY D PERRY STANTON B KEANE JOHN F | 1991 | N Eng Med1991，324，： | 1 |
| 13 | Terminal complement blockade with pexelizumab during coronary artery bypass graft surgery requiring cardiopulmonary bypass (A randomized trial) 显示文摘 | Edward D Verrier MD Stanton K | 2004 | JAMA2004，291，： | 1 |
| 14 | Uterotonic use athomebirths in low-income countries:a literature review显示文摘 | Flandermeyer D Stanton C Armbruster D | 2010 | Int JGynaecol Obstet2010，108，3： | 1 |
| 15 | Nonenzymatic browning in kiwifruit juice concertrate systems during storage显示文摘 | Wong M Stanton D W | 1998 | J of Food Sci1998，，3： | 1 |
| 16 | Central arteriovenous anastomosis for the treatment of patients with uncontrolled hy- pertension(the ROX CONTROL HTN study):a randomised co- ntrolled trial显示文摘 | Lobo M D Sobotka P A Stanton A | 2015 | Lancet2015，22，62： | 1 |
| 17 | Altered patterns of gene expression in response to myocardial infarction 显示文摘 | Stanton LW GarrardD LD Damm D et cd | 2000 | Cire Res2000，86，9： | 1 |
| 18 | The surgical management of fibrous dysplasia of bone 显示文摘 | Stanton RP Ippolito E Springfield D | 2012 | Orphanet J Rare Dis2012，7，1： | 1 |
| 19 | p38 MAP kinase signaling is necessary for rat chondrosarcoma cell proliferation 显示文摘 | Halawani D Mondeh R Stanton L A | 2004 | Oncogene2004，23，20： | 1 |
| 20 | EphA2 as a novel molecular marker and target in glioblastoma moltiform显示文摘 | Wykosky J Gibo D M Stanton C | 2005 | Mol Cancer Res2005，3，10： | 1 |
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