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| 1 | KCa channels contribute to exercise-induced coronary vasodilation in swine显示文摘 | Merkus D Sorop O Houweling B | | 0，，： | 1 |
| 2 | The Genous endothelial progenitor cell capture stent accelerates stent re-endothelialization but does not affect intimal hyperplasia in porcine coronary arteries 显示文摘 | van Beusekom HM Ertas G Sorop O | 2012 | Catheter Cardiovasc Interv2012，79，2： | 1 |
| 3 | Peripheral arteri-al tonometry cannot detect patients at low risk of coronary arterydisease显示文摘 | van den Heuvel M Sorop O Musters PJ | 2015 | Neth Heart J2015，23，10： | 1 |
| 4 | Coronary microvascular dysfunction in a porcine model of early atherosclerosis and diabetes显示文摘 | Van DM Sorop O Koopmans SJ | 2012 | Am J Physio Heart Circ2012，302，1： | 1 |
| 5 | KCa channels contribute to exercise-induced coronary vasodilation in swine显示文摘 | MERKUS D SOROP O HOUWELING B | | 0，，： | 1 |
| 6 | KCa + channels contribute to exercise-induced coronary vasodilation in swine 显示文摘 | Merkus D Sorop 0 Houweling B | 2006 | Am J Physiol Heart Circ Physiol2006，291，5： | 1 |
| 7 | Coronary vasoconstric-tor influence of angiotensin I1 is reduced in remodeled myocardi um after myocardial infarction显示文摘 | Merkus D Haitsma DB Sorop O | 2006 | Am J Physiol Heart Circ Physiol2006，291，5： | 1 |
| 8 | The Genous endothelial progenitor cell capture stent ccelerates stent re-endothelialization but does not affect intimal hyperplasia in porcine coronary arteries显示文摘 | Van Beusekom HM Ertas G Sorop O | 2012 | Catheter Cardiovasc Interv2012，79，2： | 1 |
| 9 | Role of T-type calcium channels in myogenic tone of skeletal muscle resistance arteries显示文摘 | Vanbavel E Sorop O Andreasen D | 2002 | Am J Physiol Heart Circ Physiol2002，283，6： | 1 |
| 10 | Coronary vasoconstrictor influence of angiotensin Ⅱ is reduced in remodeled myocardium after myocardial infarction显示文摘 | Merkus D Haitsma DB Sorop O | 2006 | AmJ Physiol Heart Circ Physiol2006，291，5： | 1 |
| 11 | Magnetization reversal of individual Fe nanowires in alumites studied by magnetic force microscopy显示文摘 | Sorop T G Untiedt C Luis F | 2003 | J Appl Phys2003，93，10： | 1 |
| 12 | Integrative control of coronary resistance vessel tone by endothelin and angiotensin II is altered in swine with a recent myocardial infarction 显示文摘 | Debeer V C Sorop O Pijnappels D A | 2008 | Heart Cite Physiol2008，294，5： | 1 |
| 13 | Invasive coronary imaging in animal models of atherosclerosis 显示文摘 | van Ditzhuijzen NS van den Heuvel M Sorop O | 2011 | Neth Heart2011，19，： | 1 |
| 14 | Endoscopic ultrasound-guided fine-needle aspiration pancreatic adenocarcinoma samples yield adequate DNA for next-generation sequencing:A cohort analysis显示文摘BACKGROUND Genetic tests are increasingly performed for the management of unresectable pancreatic cancer.For genotyping aimed samples current guidelines recommend using core specimens,although based on moderate quality evidence.However,in clinical practice among the endoscopic ultrasound(EUS) guided tissue acquisition methods,fine needle aspiration(FNA) is the most widely performed.AIM To assess the adequacy for next generation sequencing(NGS) of the DNA yielded from EUS-FNA pancreatic adenocarcinoma(PDAC) samples.METHODS Between November 2018 and December 2021,105 patients with PDAC confirmed by EUS-FNA were included in the study at our tertiary gastroenterology center.Either 22 gauge(G) or 19G FNA needles were used.One pass was dedicated to DNA extraction.DNA concentration and purity(A260/280,A260/230) were assessed by spectrophotometry.We assessed the differences in DNA parameters according to needle size and tumor characteristics(size,location) and the adequacy of the extracted DNA for NGS(defined as A260/280 ≥ 1.7,and DNA yield:≥ 10 ng for amplicon based NGS,≥ 50 ng for whole exome sequencing [WES],≥ 100 ng for whole genome sequencing [WGS]) by analysis of variance and ttest respectively.Moreover,we compared DNA purity parameters across the different DNA yield categories.RESULTS Our cohort included 49% male patients,aged 67.02 ± 8.38 years.The 22G needle was used in 71%of the cases.The DNA parameters across our samples varied as follows:DNA yield:1289 ng(inter quartile range:534.75-3101),A260/280 = 1.85(1.79-1.86),A260/230 = 2.2(1.72-2.36).DNA yield was > 10 ng in all samples and > 100 ng in 93% of them(one sample < 50 ng).There were no significant differences in the concentration and A260/280 between samples by needle size.Needle size was the only independent predictor of A260/230 which was higher in the 22G samples(P =0.038).NGS adequacy rate was 90% for 19G samples regardless of NGS type,and for 22G samples it reached 89% for WGS adequacy and 91% for WES and amplicon based NGS.Samples with DNA yield > 100 ng had significantly higher A260/280(1.89 ± 0.32 vs 1.34 ± 0.42,P = 0.013).Tumor characteristics were not corelated with the DNA parameters.CONCLUSION EUS-FNA PDAC samples yield DNA adequate for subsequent NGS.DNA amount was similar between 22G and 19G FNA needles.DNA purity parameters may vary indirectly with needle size. | Stefania Bunduc Bianca Varzaru Razvan Andrei Iacob Andrei Sorop Ioana Manea Andreea Spiridon Raluca Chelaru Adina Emilia Croitoru Gabriel Becheanu Mona Dumbrava Simona Dima Irinel Popescu Cristian Gheorghe | 2023 | World Journal of Gastroenterology2023，29，18： | 0 |
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