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| 1 | Study of the mo- lecular recognition of aptamers selected through ovarian cancer cell-SELEX 显示文摘 | Van Simaeys D Lopez-Colon D Sefah K | 2010 | PLoS One2010，5，13： | 1 |
| 2 | Study of the molecular recognition of aptamers se lected through ovarian cancer cell-SELEX显示文摘 | Van Simaeys D López-Colón D Sefah K Sutphen R Jimenez E Tan W | | 0，，： | 1 |
| 3 | Rapid genotyping of Achr- omobacter xylosoxidans, Acinetobacter banmannii, Klebsiella pneum- oniae, Pseudomonas aeruginosa and Stenotrophomonas maltophilia is- olates using melting curve analysis of RAPD-generated DNA fragme- nts (McRAPD)显示文摘 | Deschaght P Van Simaey L Decat E | 2011 | Res Microbiol2011，162，4： | 1 |
| 4 | Comparison of the sensitivity of culture, PCR and quantitative real - time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients 显示文摘 | Deschaght P De Baere T Van Simaey L | 2009 | BMC Microbiol2009，9，： | 1 |
| 5 | Interactive effects of salicylic acid and nitric oxide on soybean plants un der NaCl salinity显示文摘 | SIMAEI M KHAVARI NEJAD R A SAADATMAND S | 2011 | Russian Journal of Plant Physiology2011，5，58： | 1 |
| 6 | The role of surgery for local recurrence of renal Ewing's sarcoma: a case report显示文摘 | Basiri A Parvin M Simaei NR | 2006 | Urol J2006，3，4： | 1 |
| 7 | Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamers显示文摘AIM: To generate DNA-aptamers binding to Methicillinresistant Staphylococcus aureus(MRSA).METHODS: The Cell-Systematic Evolution of Ligands by Exponential Enrichment(SELEX) technology was used to run the selection against MRSA bacteria and develop target-specific aptamers. MRSA bacteria were targeted while Enterococcus faecalis bacteria were used for counter selection during that process. Binding assays to determine the right aptamer candidates as well as binding assays on clinical samples were performed through flow cytometry and analyzed using the FlowJ o software. The characterization of the aptamers was done by determination of their Kd values and determined by analysis of flow data at different aptamer concentration using Sigma Plot. Finally, the recognitionof the complex Gold-nanoparticle-aptamer to the bacteria cells was observed using transmission electron microscopy(TEM).RESULTS: During the cell-SELEX selection process, 17 rounds were necessary to generate enrichment of the pool. While the selection was run using fixed cells, it was shown that the binding of the pools with live cells was giving similar results. After sequencing and analysis of the two last pools, four sequences were identified to be aptamer candidates. The characterization of those aptamers showed that based on their Kd values, DTMRSA4 presented the best binding with a Kd value of 94.61 ± 18.82 nmol/L. A total of ten clinical samples of MRSA, S. aureus and Enterococcus faecalis were obtained to test those aptamers and determine their binding on a panel of samples. DTMRSA1 and DTMRSA3 showed the best results regarding their specificity to MRSA, DTMRSA1 being the most specific of all. Finally, those aptamers were coupled with gold-nanoparticle and their binding to MRSA cells was visualized through TEM showing that adduction of nanoparticles on the aptamers did not change their binding property.CONCLUSION: A total of four aptamers that bind to MRSA were obtained with Kd values ranking from 94 to 200 nmol/L. | Diane Turek Dimitri Van Simaeys Judith Johnson Ismail Ocsoy Weihong Tan | 2013 | World Journal of Translational Medicine2013，2，3： | 0 |
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