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| 1 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 2 | Biological function of Presenilin and its role in AD pathogenesis显示文摘Presenilins(PSs)are the catalytic core of γ-secretase complex.However,the mechanism of FAD-associated PS mutations in AD pathogenesis still remains elusive.Here we review the general biology and mechanism of γ-secretase and focus on the catalytic components–presenilins and their biological functions and contributions to the AD pathogenesis.The functions of presenilins are divided into γ-secretase dependent and γ-secretase independent ones.The γ-secretase dependent functions of presenilins are exemplified by the sequential cleavages in the processing of APP and Notch;the γ-secretase independent functions of presenilins include stabilizing β-catenin in Wnt signaling pathway,regulating calcium homeostasis and their interaction with synaptic transmission. | Shuting Zhang Mingming Zhang Fang Cai Weihong Song | 2013 | Translational Neurodegeneration2013，2，1： | 1 |
| 3 | The valuation of China’s environmental degradation from 2004 to 2017显示文摘This paper aims to evaluate the cost of environmental degradation by adopting the conventional environmental economic methodology in China from 2004 to 2017 and summarize the change in both the causes and costs of China’s environmental degradation.Results from this study revealed the following:i.The environmental degradation cost in China increased from 511 billion yuan to 1,892 billion yuan from 2004 to 2017,and its share in the GDP decreased from 3.05% to 2.23%;ii.The environmental degradation cost growth rate was lower than the GDP growth rate.The environmental degradation cost growth rate decreased sharply,by dropping from 10% in 2014 to 2% in 2017.The environmental benefits of industrial transformation have emerged;iii.The provinces of Shandong,Hebei,Jiangsu,Henan,and Guangdong had the highest environmental degradation costs.The annual average growth rate of the environmental degradation costs in Jiangsu,Guangdong,and Zhejiang were lower than their growth rate of the GDP respectively;iv.Consideration of environmental degradation cost in decision-making could contribute to the high-quality development of China. | Guoxia Ma Fei Peng Weishan Yang Gang Yan Shuting Gao Xiafei Zhou Ji Qi Dong Cao Yue Zhao Wen Pan Hongqiang Jiang Hong Jing Guangxia Dong Minxue Gao Jingbo Zhou Fang Yu Jinnan Wang | 2020 | Environmental Science and Ecotechnology2020，，1： | 1 |
| 4 | Water-dispersible nano-pollutions reshape microbial metabolism in type-specific manners:A metabolic and bacteriological investigation in Escherichia coli显示文摘Incomplete separation and recycling of nanoparticles are causing undesirable nanopollution and thus raising great concerns with regard to nanosafety.Since microorganisms are important regulator of physiological processes in many organisms,the interaction between nanopollution and microbial metabolomics and the resultant impact on the host’s health are important but unclear.To investigate how typical nanopollution perturbs microbial growth and metabolism,Escherichia coli(E.coli)in vitro was treated with six water-dispersible nanomaterials(nanoplastic,nanosilver,nano-TiO 2,nano-ZnO,semiconductor quantum dots(QDs),carbon dots(CDs))at human-/environment-relevant concentration levels.The nanomaterials exhibited type-specific toxic effects on E.coli growth.Global metabolite profiling was used to characterize metabolic disruption patterns in the model microorganism exposed to different nanopollutants.The percentage of significant metabolites(p<0.05,VIP>1)accounted for 6%–38%of the total 293 identified metabolites in each of the nanomaterial-contaminated bacterial groups.Metabolic results also exhibited significant differences between different nanopollutants and dose levels,revealing type-specific and untypical concentration-dependent metabolic responses.Key metabolites responsive to nanopollution exposures were mainly involved in amino acid and purine metabolisms,where 5,4,and 7 significant metabolic features were included in arginine and proline metabolism,phenylalanine metabolism,and purine metabolism,respectively.In conclusion,this study horizontally compared and demonstrated how typical nanopollution perturbs microbial growth and metabolomics in a type-specific manner,which broadens our understanding of the ecotoxicity of nanopollutants on microorganisms. | Shuqin Liu Rui Wu Xi Yang Shuting Fang Zhangmin Xiang Shenghong Yang Gangfeng Ouyang | 2022 | Frontiers of Environmental Science & Engineering2022，16，9： | 0 |
| 5 | Transcriptomic and genetic approaches reveal that low-light-induced disease susceptibility is related to cellular oxidative stress in tomato显示文摘The impact of low light intensities on plant disease outbreaks represents a major challenge for global crop security,as it frequently results in significant yield losses.However,the underlying mechanisms of the effect of low light on plant defense are still poorly understood.Here,using an RNA-seq approach,we found that the susceptibility of tomato to Pseudomonas syringae pv.tomato DC3000(Pst DC3000)under low light was associated with the oxidation-reduction process.Low light conditions exacerbated Pst DC3000-induced reactive oxygen species(ROS)accumulation and protein oxidation.Analysis of gene expression and enzyme activity of ascorbate peroxidase 2(APX2)and other antioxidant enzymes revealed that these defense responses were significantly induced by Pst DC3000 inoculation under normal light,whereas these genes and their associated enzyme activities were not responsive to pathogen inocula-tion under low light.Additionally,the reduced ascorbate to dehydroascorbate(AsA/DHA)ratio was lower under low light compared with normal light conditions upon Pst DC3000 inoculation.Furthermore,the apx2 mutants generated by a CRISPR-Cas9 gene-editing approach were more susceptible to Pst DC3000 under low light conditions.Notably,this increased susceptibility could be significantly reduced by exogenous AsA treatment.Collectively,our findings suggest that low-light-induced disease susceptibility is associated with increased cellular oxidative stress in tomato plants.This study sheds light on the intricate relationship between light conditions,oxidative stress,and plant defense responses,and may pave the way for improved crop protection strategies in low light environments. | Qian Luo Jiao Wang Ping Wang Xiao Liang Jianxin Li Changqi Wu Hanmo Fang Shuting Ding Shujun Shao Kai Shi | 2023 | Horticulture Research2023，10，10： | 0 |
| 6 | Comparison of COSMO-SAC Model and DFT Quantum Chemical Model in Molecular Design of IL Extractant显示文摘ILs agents with better extraction efficiency for desulfurization were screened by simulation in this paper.The COSMO-SAC model and the density functional theory(DFT)were used to select the prospective solvents from a wide variety of ionic liquids suitable for extractive distillation.The COSMO-SAC model and the density functional theory(DFT)were used to study the inconsistencies of the efficiency of extractive separation during the simulation.Six ILs solvents with better extraction efficiency were screened out by the COSMO-SAC model.And the six kinds of ionic liquids based on the extraction efficiency order determined by the COSMO-SAC model were reordered based on the DFT model.The results showed that the order of desulfurization efficiency based on the two kinds of models was different.Experiments were carried out to verify the accuracy of the two sequences.The order of the above six ILs related with the extractive desulfurization efficiency was studied by experimental researching.The results show that the extraction efficiency sequence of the six ILs obtained by the experiment is consistent with the order of simulation results based on the DFT model.The difference between the COSMO-SAC model and the DFT model was analyzed and interpreted from the calculation principle aspect at the end of this paper. | Fang Jing Wang Yijing Zhang Shuting Zheng Wenwen Li Hao Li Chunli | 2019 | China Petroleum Processing & Petrochemical Technology2019，21，3： | 0 |
| 7 | Rare variants in FANCJ induce premature ovarian insufficiency in humans and mice显示文摘Premature ovarian insufficiency(POI)is a severe female reproductive disorder that affects 1%of women in general populations(European Society for Human Reproduction and Embryology[ESHRE]Guideline Group on POI et al.,2016).An increasing prevalence up to 3.7%has been reported in a recent meta-analysis(Golezar et al.,2019).POl can lead to infertility or subfertility,as well as a range of complex complications suffering multi-organ systems,seriously threatening women's health and reducing the life quality.By contrast,POl is a representative heterogeneous disease with multiple etiologies.While more than 70 causative POI genes have been identified,the etiology of more than half of the POI patients is still ambiguous(Jiao et al.,2018).Unreported POI causative genes,therefore,remain to be identified. | Xi Yang Shuting Ren Jialin Yang Yuncheng Pan Zixue Zhou Qing Chen Yunzheng Fang Lingyue Shang Feng Zhang Xiaojin Zhang Yanhua Wu | 2024 | Journal of Genetics and Genomics2024，51，2： | 0 |
| 8 | PHD3 inhibits colon cancer cell metastasis through the occludin-p38 pathway显示文摘Prolyl hydroxylase 3(PHD3)hydroxylates HIFαin the presence of oxygen,leading to HIFαdegradation.PHD3 inhibits tumorigenesis.However,the underlying mechanism is not well understood.Herein,we demonstrate that PHD3 inhibits the metastasis of colon cancer cells through the occludin-p38 MAPK pathway independent of its hydroxylase activity.We find that PHD3 inhibits colon cancer cell metastasis in the presence of the PHD inhibitor DMOG,and prolyl hydroxylase-deficient PHD3(H196A)suppresses cell metastasis as well.PHD3 controls the stability of the tight junction protein occludin in a hydroxylase-independent manner.We further find that PHD3-inhibited colon cancer cell metastasis is rescued by knockdown of occludin and that occludin acts as a negative regulator of cell metastasis,implying that PHD3 suppresses metastasis through occludin.Furthermore,knockdown of occludin induces phosphorylation of p38 MAPK,and the p38 inhibitor SB203580 impedes cell migration and invasion induced by occludin knockdown,indicating that occludin functions through p38.Moreover,knockdown of occludin enhances the expression of MKK3/6,the upstream kinase of p38,while overexpression of occludin decreases its expression.Our results suggest that PHD3 inhibits the metastasis of colon cancer cells through the occludin-p38 pathway independent of its hydroxylase activity.These findings reveal a previously undiscovered mechanism underlying the regulation of cancer cell metastasis by PHD3 and highlight a noncanonical hydroxylaseindependent function of PHD3 in the suppression of cancer cells. | Yuyao Li Tanglong Yuan Hongwei Zhang Shuting Liu Jie Lun Jing Guo Yu Wang Yuying Zhang Jing Fang | 2023 | Acta Biochimica et Biophysica Sinica2023，55，11： | 0 |
| 9 | A molecular toolkit to boost functional genomic studies in transformation-recalcitrant vegetable legumes显示文摘Dear Editor Legumes,the second-largest family of crops,contribute over one-third of human dietary proteins.Soybean(Glycine max L.),common bean(Phaseolus vulgaris L.),pea(Pisum sativum L.),and cowpea(Vigna unguiculata L.)are among the most widely culti-vated crop legumes for grain and vegetable and are essential for food security globally. | Xinyang Wu Peipei Zhang Shuting Chen Zixin Zhang Yihan Zhang Pingping Fang Kang Ning Ting Sun Pei Xu | 2023 | Horticulture Research2023，10，5： | 0 |
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