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| 1 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001,46,23: | 6 |
| 2 | Expression analysis of gdcsP promoter from C_3-C_4 intermediate plant Flaveria anomala in transgenic rice显示文摘The gdcsP promoter isolated from C3-C4 intermediate plant Flaveria anomala was fused to the β-glu-curonidase (GUS) gene. The chimeric gene was inserted into the binary vector pBin19 and introduced into the rice (Oryza saliva L.) cv. 8706 by Agrobacterium-mediated gene transfer. GUS activity can be detected in leaf, leaf sheath, stem and root tissues via fluorometric GUS assay. However, no GUS activity was found in mature endosperm. Histochemical localization revealed that GUS expression was exclusively restricted to vascular tissues in transgenic plants. This promoter also showed spatial-temporal expression patterns that GUS expression declined significantly with the maturity of plants. These expression patterns make the gdcsP promoter extremely valuable in the applied biotechnology that needs target gene expression restricted to vascular tissues. | CHEN Shuai, QU Nan, CAO Shouyun, Hermann Bauwe. CHEN Shouyi, TIAN Wenzhong & CHU Chengcai1. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China 2. Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany 3. Rostock University, 18055 Rostock, Germany | 2001 | Chinese Science Bulletin2001,46,19: | 3 |
| 3 | A new luminescent metal-organic framework for selective sensing of nitroaromatic explosives显示文摘A microporous luminescent metal-organic framework [Zn_4L_2(H_2O)_2].(H_2O)_m(DMA)_n(1)(H_4L=5,5'-((1H-pyrazole-3,5-dicarbonyl)bis(azanediyl))diisophthalic acid, DMA=N,N-dimethylacetamide) was synthesized and characterized by infrared radiation(IR), thermogravimetric analyses(TGA), powder X-ray diffraction spectra(PXRD) and X-ray diffraction. Complex 1has a three dimensional(3D) framework, which can be simplified as 5,5,5,5-c net with the Schlfi symbol of {43.64.83}{44.65.8}{45.65}2. This luminescent metal-organic framework(MOF) shows selectively sensitive to nitrobenzene and series of nitroaromatic explosives such as 4-nitrotoluene, 1,4-dinitrobenzene, 1,3-dinitrobenzene and 2,4-dinitrotoluene, and exhibits well recyclability. So complex 1 could be used to detect nitroaromatic explosives as a selective sensing material. | Tingting Wang Yanyuan Jia Qiang Chen Rui Feng Shouyi Tian Tong-Liang Hu Xian-He Bu | 2016 | Science China Chemistry2016,59,8: | 3 |
| 4 | Cloning and expression of putative ethylene receptor genes in soybean plant显示文摘Ethylene plays important roles in plant growth, development, and stress responses, and ethylene receptors have been identified and studied extensively in various plant species. Here we report the cloning of four ethylene receptor genes from soybean, i.e. GmETR1, GmERS1, GmETR2 and GmEIN4. Construction of the phylogenic tree showed that GmETR1 and GmERS1 belong to subfamily I whereas GmETR2 and GmEIN4 belong to subfamily II. The four ethylene receptor genes showed different tissue-specific expression patterns in roots, stems, leaves, cotyledons, flowers, pods and seeds of soybean. These genes were differentially regulated by various abiotic stresses and plant hormones. The possible roles of the four genes in soybean plant were also discussed. | Xie Zongming Lei Gang Wuriyanghan Hada Tian Aiguo Zhang Jinsong Chen Shouyi | 2007 | Progress in Natural Science:Materials International2007,17,10: | 1 |