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| 1 | A telomere-to-telomere gap-free reference genome of watermelon and its mutation library provide important resources for gene discovery and breeding显示文摘Watermelon,Citrullus lanatus,is the world's third largest fruit crop.Reference genomes with gaps and a narrow genetic base hinder functional genomics and genetic improvement of watermelon.Here,we report the assembly of a telomere-to-telomere gap-free genome of the elite watermelon inbred line G42 by incorporating high-coverage and accurate long-read sequencing data with multiple assembly strategies.All 11 chromosomes have been assembled into single-contig pseudomolecules without gaps,representing the highest completeness and assembly quality to date.The G42 reference genome is 369321829 bp in length and contains 24205 predicted protein-coding genes,with all 22 telomeres and 11 centromeres characterized.Furthermore,we established a pollen-EMS mutagenesis protocol and obtained over 200000M1 seeds from G42.In a sampling pool,48 monogenic phenotypic mutations,selected from 223M1and 78 M2 mutants with morphological changes,were confirmed.The average mutation density was 1 SNP/1.69Mband1 indel/4.55 Mb per M1 plant and 1SNP/1.08Mb and 1 indel/6.25 Mb per M2 plant.Taking advantage of the gap-free G42 genome,8039 mutations from 32 plants sampled from M1 and M2 families were identified with 100%accuracy,whereas only 25% of the randomly selected mutations identified using the 97103v2 reference genome could be confirmed.Using this library and the gap-free genome,two genes responsible for elongated fruit shape and male sterility(CiMs1)were identified,both caused by a single basechange from G to A.The validated gap-free genome and its EMS mutation library provide invaluable resources for functional genomics and genetic improvement of watermelon. | Yun Deng Shoucheng Liu Yilin Zhang Jingsheng Tan Xiaopeng Li Xiao Chu Binghua Xu Yao Tian Yudong Sun Bosheng Li YunbiXu Xing Wang Deng Hang He Xingping Zhang | 2022 | Molecular Plant2022，15，8： | 9 |
| 2 | Isolation,Identification,and Characterization of an Aluminum-Tolerant Bacterium Burkholderia sp.SB1 from an Acidic Red Soil显示文摘Aluminum(Al) toxicity usually occurs in acidic soils worldwide, which is detrimental to the growth of organisms. An Al-tolerant bacterium, SB1, was isolated from an acidic red soil of Chingkang Mountain, located in Jiangxi Province of China. Polyphasic analysis,including a 16 S rDNA phylogenetic tree as well as morphological and physicochemical properties, revealed that the isolate was a gramnegative, rod-shaped bacterium, which was recognized as Burkholderia sp. SB1 and had extreme acidity tolerance(pH 2.2) and excellent Al resistance(270 mg L^(-1) Al^(3+)). It could remove Al by up to 97.7% at a concentration of 54 mg L^(-1) Al^(3+). SB1 behavior under different temperatures and antibiotics was also examined. SB1 preferred moderate temperature conditions, ranging from 25 to37?C, and exhibited notable resistance to multiple antibiotics(including ampicillin, streptomycin, and tetracycline), except for being sensitive to chloramphenicol. Therefore, as the first reported bacterium to possess favorable Al resistance and excellent Al removal,Burkholderia sp. SB1 can potentially be used as an agent for bioremediation of Al-contaminated acidic red soils. | HUANG Shoucheng WANG Xiaodong LIU Xu HE Genhe WU Jichun | 2018 | Pedosphere2018，28，6： | 4 |
| 3 | Relationship among subtropical anticyclone,drought and earth rotation显示文摘The deep reason of severe disaster weather with the relationship among the earth nutation,rotation and atmospheric change is explored based on the effective results about the disaster weather prediction of the long term made by the variation of the earth rotation in near 10 years. It is discussed the relationship between the subtropical anticyclone and subtropical easterlies to aim at the problem of high temperature and drought in the globe,further more,the comparison analysis has been made to the earth nutation and the variation of rotation. The research results show that the reasons of severe disaster weather not only are due to atmosphere itself,but have some variation information of earth movement which could be used for the weather forecast. | Wei Ming OuYang Shoucheng Fei Haiyan | 2008 | Engineering Sciences2008，6，4： | 3 |
| 4 | Discussion on Super High-temperature Weather and City Construction显示文摘With the method of structure analysis, this work analyzes the real data of the high-temperature weather of China in 2003 and those in history, and finds out that the structure character of the high-temperature weather process corresponds to the distribution of urban buildings. The result shows that excessive dense buildings could influence the atmosphere structure, which leads to the urban temperature increasing sharply. On the other hand, the structure analysis also reveals some problems on urban construction, and the corresponding countermeasure is an efficient method for high-temperature weather forecast. | OuYang Shoucheng Wei Ming | 2006 | 工程科学(英文版)2006，4，1： | 3 |
| 5 | Lithosphere architecture characterized by crust±mantle decoupling controls the formation of orogenic gold deposits显示文摘This study,via combined analysis of geophysical and geochemical data,reveals a lithospheric architecture characterized by crust-mantle decoupling and vertical heat-flow conduits that control orogenic gold mineralization in the Ailaoshan gold belt on the southeastern margin of Tibet.The mantle seismic tomography indicates that the crust-mantle decoupled deformation,defined from previous seismic anisotropy analysis,was formed by upwelling and lateral flow of the asthenosphere,driven by deep sub duction of the Indian continent,Our magnetotelluric and seismic images show both a vertical conductor across the Moho and high Vp/Vs anomalies both in the uppermost mantle and lowest crust,suggesting that crust-mantle decoupling promotes ponding of mantle-derived basic melts at the base of the crust via a heat-flow conduit.Noble gas isotope and halogen ratios of gold-related ore minerals indicate a mantle source of ore fluid.A rapid decrease in Cl/F ratios of lamprophyres under conditions of 1.2 GPa and1050℃suggests that the ore fluid was derived from degassing of the basic melts.Similar lithospheric architecture is recognized in other orogenic gold provinces,implying analogous formational controls. | Zengqian Hou Qingfei Wang Haijiang Zhang Bo Xu Nian Yu Rui Wang David IGroves Yuanchuan Zheng Shoucheng Han Lei Gao Lin Yang | 2023 | National Science Review2023，10，3： | 2 |
| 6 | Quantitative Analysis of Relationship Between Pore Structure and Compressive Strength of Foamed Concrete显示文摘The effect of dry density,water-cement ratio,the addition of fly ash,and sand content on the porosity and pore distribution of foamed concrete is investigated.Digital microscopy and Image J software are employed to examine the landscape of pores with different sizes.Based on the Balshin empirical formula,a mathematical model is established to quantitatively predict the relationship between the pore structures and the compressive strength of foamed concrete.The results well demonstrate that there is a significant correlation between the modified formula and empirical parameters. | Geng Fei Yin Wanyun Xie Jianguang Liu Shoucheng Li Haoran Gui Jingneng | 2018 | Transactions of Nanjing University of Aeronautics and Astronautics2018，35，3： | 2 |
| 7 | New PVK-based nonlinear optical polymers:Enhanced nonlinearity and improved transparency 显示文摘 | Zhong'an Li Shoucheng Dong Pengcheng Li | 2008 | Journal of Polymer Science : Part A : Polymer Chemistry2008，9，： | 1 |
| 8 | Evolution science and infrastructural analysis of the second Stir 显示文摘 | Ouyang Shoucheng Lin Y Wang Z | 2001 | Kybernetes2001，30，4： | 1 |
| 9 | Conjecture on law of conservation of informational infrastructure显示文摘 | REN Zhenqiu LIN Yi OUYANG Shoucheng | 1998 | Kybernetes-The International Journal of Systems & Cybernetics1998，75，5： | 1 |
| 10 | The liquid flow measuring device based on AT89C51 microcontroller显示文摘 | Ding Shoucheng | 2012 | Advanced Science Letters2012，11，1： | 1 |
| 11 | Photoluminescence properties and thermal stability of Eu^(3+)-activated La_(7)Ta_(3)W_(4)O_(30) red-emitting phosphors for near-UV-excited w-LEDs显示文摘Novel trivalent europium(Eu^(3+))-activated La_(7)Ta_(3)W_(4)O_(30):xEu^(3+)(x=0.5 mol%-40 mol%) red-emitting phosphors were synthesized by means of a high-temperature solid-state reaction.The structure,morphology,photoluminescence,thermal-stability properties,lifetime,and color-rendering of the prepared phosphors were investigated in detail.The La_(7)Ta_(3)W_(4)O_(30):Eu^(3+) phosphors show five emission peaks under near-ultraviolet(n-UV) at 397 nm,and these peaks are ascribed to the transitions of ^(5)D0-^(7)Fj(j=0,1,2,3 and 4) by Eu^(3+) ions.The optimal doping concentration of Eu^(3+) is 20 mol%,and the critical distance of the energy transfer between the Eu^(3+)ions was calculated to be 1.768 nm.The quenching temperature(T_(0.5)) of La_(7)Ta_(3)W_(4)O_(30):20 mol%Eu^(3+) is about 440 K.The quantum yield(QY) was measured to be 85.85%.The fabricated white-light-emitting diodes(w-LEDs) possess high color-rendering index(R_(a)) of 90,and high correlated color temperature(CCT) of 5810 K,respectively.The Commission Internationale de L’Eclairage(CIE) coordinates are(0.311,0.322).Therefore,the prepared phosphor has a promising application for w-LEDs. | Yu Ren Bin Deng Ting Liang Shuang Shu Junlan Guo Shoucheng Zhao Ruijin Yu | 2021 | Journal of Rare Earths2021，39，8： | 1 |
| 12 | Topological insulators and superconductors显示文摘 | QI Xiaoliang ZHZNG Shoucheng | 2011 | Rev Mod Phys2011，83，4： | 1 |
| 13 | Quantum spin hall effect and topological phase transition in HgTe quantum wells显示文摘 | BERNEVIG B A HUGHES T L ZHANG Shoucheng | 2006 | Science2006，314，5806： | 1 |
| 14 | About spin and evolution: introduction, rotation and evolution 显示文摘 | Ouyang Shoucheng | 2009 | Scientific Inquiry2009，10，2： | 1 |
| 15 | The effect of collagen-chi- tosan porous scaffold thickness on dermal regeneration in a one-stage grafting procedure显示文摘 | Haifei S Xingang W Shoucheng ' | 2014 | J Mech Bchav Biomed Mater2014，29，： | 1 |
| 16 | The quantum spin Hall effect and topological insulators 显示文摘 | Qi Xiaoliang Zhang Shoucheng | 2010 | Physics Today2010，63，1： | 1 |
| 17 | Reviews on the research status and prospect of the pre- fabricated concrete shear wall structure显示文摘 | Ding Kewei Ling Ru Yin Wanyun Liu Shoucheng Ni Shumei | 2013 | Applied Mechanics and Materials2013，291，： | 1 |
| 18 | Preparationand indoor formaldehyde photocatalytic property of Cu -Ce / Ti O2显示文摘 | Zhang Hao Liu Shoucheng Hu Yi | 2015 | Chinese Rare Earths2015，36，5： | 1 |
| 19 | Identification and analysis of mouse non-coding RNA using transcriptome data显示文摘Transcripts are expressed spatially and temporally and they are very complicated, precise and specific; however, most studies are focused on protein-coding related genes. Recently, massively parallel c DNA sequencing(RNA-seq) has emerged to be a new and promising tool for transcriptome research, and numbers of non-coding RNAs, especially linc RNAs, have been widely identified and well characterized as important regulators of diverse biological processes. In this study, we used ultra-deep RNA-seq data from 15 mouse tissues to study the diversity and dynamic of non-coding RNAs in mouse. Using our own criteria, we identified totally 16,249 non-coding genes(21,569 non-coding RNAs) in mouse. We annotated these non-coding RNAs by diverse properties and found non-coding RNAs are generally shorter, have fewer exons, express in lower level and are more strikingly tissue-specific compared with protein-coding genes. Moreover, these non-coding RNAs show significant enrichment with transcriptional initiation and elongation signals including histone modifications(H3K4me3, H3K27me3 and H3K36me3), RNAPII binding sites and CAGE tags. The gene set enrichment analysis(GSEA) result revealed several sets of linc RNAs associated with diverse biological processes such as immune effector process, muscle development and sexual reproduction. Taken together, this study provides a more comprehensive annotation of mouse non-coding RNAs and gives an opportunity for future functional and evolutionary study of mouse non-coding RNAs. | Yuhui Zhao Wanfei Liu Jingyao Zeng Shoucheng Liu Xinyu Tan Hasanawad Aljohi Songnian Hu | 2016 | Science China(Life Sciences)2016，59，6： | 1 |
| 20 | Pricing Life Insurance:Combining Economic,Finan-cial ,and Actuarial Approaches显示文摘 | Mao Hong Carson James M Ostaszewski Krzysztof M Shoucheng Luo | 2004 | Journal of Insurance Issues2004，27，2： | 1 |
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