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| 1 | A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals显示文摘The Middle East respiratory syndrome(MERS)is a lethal zoonosis caused by MERS coronavirus(MERS-CoV)and poses a significant threat to public health worldwide.Therefore,a rapid,sensitive,and specific serologic test for detecting anti-MERS-CoV antibodies in both humans and animals is urgently needed for the successful management of this illness.Here,we evaluated various novel luciferase immunosorbent assays(LISA)based on nucleocapsid protein(NP)as well as fragments derived from spike protein(S)including subunit 1(S1),N terminal domain(NTD),receptorbinding domain(RBD)and subunit 2(S2)of S for the detection of MERS-CoV-specific IgG.Fusion proteins,including nanoluciferase(NLuc)and various fragments derived from the NP or S protein of MERS-CoV,were expressed in human embryonic kidney 293 T cells.LISAs that detected anti-MERS-CoV IgG were further developed using cell lysates expressing various fusion proteins.Panels of human or animal samples infected with MERS-CoV were used to analyze the sensitivity and specificity of various LISAs in reference to a MERS-CoV RT-PCR,commercial S1-based ELISA,and pseudovirus particle neutralization test(ppNT).Our results showed that the S1-,RBD-,and NP-LISAs were more sensitive than the NTD-and S2-LISAs for the detection of anti-MERS-CoV IgG.Furthermore,the S1-,RBD-,and NP-LISAs were more sensitive(by at least 16-fold)than the commercially available S1-ELISA.Moreover,the S1-,RBD-,and NPLISA specifically recognized anti-MERS-CoV IgG and did not cross-react with samples derived from other human CoV(OC43,229E,HKU1,NL63)-infected patients.More importantly,these LISAs proved their applicability and reliability for detecting anti-MERS-CoV IgG in samples from camels,monkeys,and mice,among which the RBD-LISA exhibited excellent performance.The results of this study suggest that the novel MERS-CoV RBD-and S1-LISAs are highly effective platforms for the rapid and sensitive detection of anti-MERS-CoV IgG in human and animal samples.These assays have the potential to be used as serologic tests for the management and control of MERS-CoV infection. | Wenling Wang Tianyu Wang Yao Deng Peihua Niu Ruhan A Jincun Zhao Malik Peiris Shixing Tang Wenjie Tan | 2019 | Biosafety and Health2019，1，3： | 1 |
| 2 | In-situ energy calibration of SSRF macromolecular crystallography beamline using powder diffraction显示文摘For X-ray powder diffraction spectra collected by an area detector of MarCCD on macromolecular crystallography beamline of SSRF,an energy calibration method was developed using LaB_6(660a) powder diffraction for in-situ rapid energy calibration of the X-rays without changing the experimental conditions.The intensity of each diffraction ring was integrated,and the accurate peak positions were fitted by Pseudo-Voigt function model.The sample's interplanar spacing for XRD analysis and the calibrated energy were obtained by the PCPDFWIN code and by fitting all the energies with the least-square method.The exposure time and the sample-to-detector distance were found no effect on accuracy of the energy calibration,and the in-situ energy calibration could be done with an accuracy of better than 0.4‰in 7-18 keV.This method is applicable to other X-ray beamlines. | Tao Shixing Zhu Jing Niu Jing Chen Mingzhi Liu Ke Wang Yu Wang Qisheng Sun Bo Huang Sheng Tang Lin He Jianhua | 2010 | Nuclear Science and Techniques2010，21，6： | 1 |
| 3 | Human Immunodeficiency Virus Type 1 N-Terminal Capsid Mutants That Exhibit Aberrant Core Morphology and Are Blocked In Initiation of Reverse Transcription in Infected Cells显示文摘 | Shixing Tang Tsutomu Murakarni Beth E Agresta | 2001 | Journal of Virology Oct2001，75，： | 1 |
| 4 | Development and Evaluation of a Universal and Supersensitive NS1-Based Luciferase Immunosorbent Assay to Detect Zika Virus-Specific IgG显示文摘Zika virus(ZIKV) causes rash, moderate fever, conjunctivitis, and arthralgia, and has serious connection with neurological complications;therefore, it is a major threat to public health. A rapid and supersensitive method for detecting anti-ZIKV antibodies in humans and animals is thus urgently required. Here, we report an NS1-based luciferase immunosorbent assay(LISA), developed to detect ZIKV-specific IgG. Fusion proteins including a reporter Nano-luciferase(NLuc) and various fragments of ZIKV NS1 protein were expressed in 293 T cells. LISA was performed using the above cell lysates containing the expressed fusion proteins. Sample panels of humans and animals infected with ZIKV were examined for sensitivity of LISA, relative to those of ZIKV RT-PCR, commercial NS1-based ELISA, and micro-neutralization(MN) assays.Specificity and potential cross-reactivity were also evaluated using various convalescent serum samples derived from patients infected with dengue virus(DENV), Japanese encephalitis virus(JEV), and hepatitis C virus(HCV). Results indicated the optimal antigenic domain for anti-ZIKV IgG detection was located within 172–352 amino acids(aa) of ZIKV NS1 protein. NS1-based LISA performs better than commercial ELISA in anti-ZIKV Ig G detection. LISA was shown to be at least fourfold more sensitive than commercial ELISA, and could detect anti-ZIKV Ig G in various animal hosts without the need of species-specific labeled antibody. This novel assay is potentially useful for the rapid and sensitive detection of anti-ZIKV IgG in human and animal samples. | Tianyu Wang Ying Zhan De Wu Zhihai Chen Wei Wu Yao Deng Wenling Wang Wenjie Tan Shixing Tang | 2020 | Virologica Sinica2020，35，1： | 0 |
| 5 | A novel 3D printed technology to construct a monolithic ultrathin nanosheets Co_(3)O_(4)/SiO_(2) catalyst for benzene catalytic combustion显示文摘In this study,a novel three-dimensional(3D)-OMm-Co_(3)O_(4)/SiO_(2)-0.5AP(OMm=ordered macro–meso porous,AP=aluminum phosphate)monolithic catalyst was for the first time constructed successfully with the hierarchical Co-phyllosilicate ultrathin nanosheets growth on the surface of 3D printed ordered macropore–mesoporous SiO_(2)support.On the one hand,we discovered that the construction of ordered macropore–mesoporous structures is beneficial to the diffusion and adsorption of reactants,intermediates,and products.On the other hand,the formation of hierarchical Co-phyllosilicate ultrathin nanosheets could provide more active Co&+species,abundant acid sites,and active oxygen.The above factors are in favor of improving the catalytic performance of benzene oxidation,and then a 3D-OMm-Co_(3)O_(4)/SiO_(2)-0.5AP catalyst exhibited the superior catalytic activity.To explore the effect of catalysts structure and morphology,various Co-based catalysts were also constructed.Simultaneously,the 3D-OMm-Co_(3)O_(4)/SiO_(2)-0.5AP catalyst has excellent catalytic performance,water resistance,and thermal stability in the catalytic combustion of benzene due to the strong interactions between Co&+species and SiO_(2)in the phyllosilicate.Therefore,this study proposes a new catalyst synthesis method through 3D printing,and presents considerable prospects for the removal of VOCs from industrial applications. | Yuntai Xi Fang Dong Xin Xu Shixing Wu Zhicheng Tang Jiyi Zhang | 2023 | Nano Research2023，16，10： | 0 |
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