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| 1 | Two-dimensional gel electrophoresis in bacterial proteomics显示文摘Two-dimensional gel electrophoresis(2-DE)is a gel-based technique widely used for analyzing the pro-tein composition of biological samples.It is capable of resolving complex mixtures containing more than a thousand protein components into individual protein spots through the coupling of two orthogonal bio-physical separation techniques:isoelectric focusing(first dimension)and polyacrylamide gel electrophore-sis(second dimension).2-DE is ideally suited for ana-lyzing the entire expressed protein complement of a bacterial cell:its proteome.Its relative simplicity and good reproducibility have led to 2-DE being widely used for exploring proteomics within a wide range of envi-ronmental and medically-relevant bacteria.Here we give a broad overview of the basic principles and his-torical development of gel-based proteomics,and how this powerful approach can be applied for studying bacterial biology and physiology.We highlight specific 2-DE applications that can be used to analyze when,where and how much proteins are expressed.The links between proteomics,genomics and mass spectrometry are discussed.We explore how proteomics involving tandem mass spectrometry can be used to analyze(post-translational)protein modifications or to identify proteins of unknown origin by de novo peptide se-quencing.The use of proteome fractionation tech-niques and non-gel-based proteomic approaches are also discussed.We highlight how the analysis of pro-teins secreted by bacterial cells(secretomes or exo-proteomes)can be used to study infection processes or the immune response.This review is aimed at non-specialists who wish to gain a concise,compre-hensive and contemporary overview of the nature and applications of bacterial proteomics. | Shirly O.T.Curreem Rory M.Watt Susanna K.P.Lau Patrick C.Y.Woo | 2012 | Protein & Cell2012，3，5： | 4 |
| 2 | 天福自走式TMR饲料搅拌机显示文摘自走式TMR饲料搅拌机是以农用汽车为载体,结合现有TMR设备自主研发的一款集搅拌、混合、撒料于一体的新型TMR饲料搅拌车。此设备饲喂方便快捷,一机多用,可设计双侧开门,能将各种干草、农作物秸秆、青贮等纤维饲料和精饲料直接混合饲喂,对提高饲养场空间利用率和生产管理、生产效率有显著作用。适用于农牧业养殖区、大中型饲养场和规范社区饲养场的饲料搅拌及喂养作业。 | Shirly | 2015 | 农业机械(导购)2015，0，7： | 2 |
| 3 | The effect of a new multimodal periop- erative anesthetic regimen on postoperatiwe pain, side effects,rehabilitation, and lenth of hospital slay after total joint arthroplasty显示文摘 | Peters CL Shirly B | 2006 | J Arthroplasty2006，21，62： | 1 |
| 4 | Androgen receptor expression is greater with implications for atherosclerosis 显示文摘 | McCrohon JA Alison KD Shirly N | 2000 | Circulation2000，101，2： | 1 |
| 5 | Genetic modificationof iron metabolism in mice affects the gut microbiota 显示文摘 | KEME B R SHIRLY M B YARL D P | 2012 | Biometals2012，25，： | 1 |
| 6 | Selective feeding and biodeposition by zebra mussels and their relation to changes in phytoplankton composition and seston load显示文摘 | Baker J S Levinton J P | 1998 | J Shellfish Res1998，17，4： | 1 |
| 7 | Use of Immunochemical Techniques for the Analysis of Pesticides显示文摘 | FRIEIA J SHIRLY J G ROBERT O H | 1989 | Pestic Sci1989，26，3： | 1 |
| 8 | Managing nausea andvomiting:Current strategies显示文摘 | Kitty G Kayo T Shirly W | 2003 | Care Nurse2003，23，1： | 1 |
| 9 | A city initiative to improve the quality of life for urban youth: how evaluation contributed to efective social programming显示文摘 | Rex S Green Peter M Ellis Shirly S Lee | 2005 | Evaluation and Program Planning2005，，1： | 1 |
| 10 | A possible role for secreted ferritin in tissue iron distribution显示文摘 | ESTHER G MEYRON H SHIRLY M B | 2011 | J Neural Transm2011，118，： | 1 |
| 11 | 雷沃谷神GE60(4LZ-6E3)型谷物联合收割机显示文摘雷沃谷神GE60配装雷沃动力、玉柴、全柴97.8kW(133hp)发动机,强劲的动力系统能够保障机器作业更顺畅:它还采用加长脱离分离滚筒,脱离分离能力更强,作业效率高:配装920mm加宽过桥,300mm加宽排草口,喂入通道通畅,输送能力增强,不堵塞,作业速度快、效率高;清选室加宽到920mm。 | Shirly | 2015 | 农业机械(导购)2015，0，9： | 1 |
| 12 | The impact of comfort control on air conditioner energy use in humid climates显示文摘 | HI Henderson K Rengaraj ian D B Shirly | 1992 | ASHR -AE Trans1992，98，2： | 1 |
| 13 | A factorial design experiment as a pilot study for noninvasive genetic sampling显示文摘 | SHARON RENAN EDITH SPEYER NAAMA SHAHAR TOMER GUETA ALAN R. TEMPLETON SHIRLI BAR‐DAVID | 2012 | Molecular Ecology Resources2012，，： | 1 |
| 14 | Heat wave fast ignition in inertial confinement energy显示文摘An accelerated micro-foil is used to ignite a pre-compressed cylindrical shell containing deuterium–tritium fuel.The well-known shock wave ignition criterion and a novel criterion based on heat wave ignition are developed in this work.It is shown that for heat ignition very high impact velocities are required.It is suggested that a multi-petawatt laser can accelerate a micro-foil to relativistic velocities in a very short time duration(picosecond)of the laser pulse.The cylindrical geometry suggested here for the fast ignition approach has the advantage of geometrically separating the nanosecond lasers that compress the target from the picosecond laser that accelerates the foil.The present model suggests that nuclear fusion by micro-foil impact ignition could be attained with currently existing technology. | Shalom Eliezer Shirly Vinikman Pinhasi | 2013 | High Power Laser Science and Engineering2013，1，1： | 1 |
| 15 | Challenges in the successful control of the avian coccidian显示文摘 | SHIRLY M W SMITH A L BLAKE D P | 2007 | Vaccine2007，25，： | 1 |
| 16 | Challenges in the success- ful control of the avian coccidian 显示文摘 | Shirly MW Smith AL Blake DP | 2007 | Vaccine2007，25，： | 1 |
| 17 | A microfacet based BRDF generator显示文摘 | Michael Ashikhmin Simon Premoze Perter Shirly | 2000 | In Proceeding of SIGGRAPH2000，6，： | 1 |
| 18 | A possible role for secreted ferritin in tissue iron distribution显示文摘 | Esther G Meyron H Shirly M B | | 0，，： | 1 |
| 19 | The influence of uncertainty in illness,stress apprasial,and hope on coping in survivors of breast cancer显示文摘 | Tipaporn M Shirly M Carol M | 2000 | Cancer Nursing2000，23，6： | 1 |
| 20 | Expression of the Ubiquitin Ligase Subunit Cyclin Kinase Subunit 1 and its Relationship to S-Phase Kinase Protein 2 and p27 Kip1 in Prostate Cancer显示文摘 | Ma’anit Shapira Ofer Ben-Izhak Merav Slotky Oleg Goldin Shirly Lahav-Baratz Dan D. Hershko | 2006 | The Journal of Urology2006，，5： | 1 |
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