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| 1 | Hepcidin/ferroportin expression levels involve efficacy of pegylated-interferon plus ribavirin in hepatitis C virusinfected liver显示文摘AIM:To investigate the relationship between the ironmetabolism-related gene expression profiles and efficacy of antiviral therapy in chronic hepatitis C patients.METHODS:The hepatic expression profile of ironmetabolism-related genes was analyzed and its association with virological response to pegylated-interferon plus ribavirin combination therapy was evaluated.A hundred patients with chronic hepatitis C(genotype1b,n = 50; genotype 2,n = 50) were enrolled and retrospectively analyzed.Liver biopsy samples were subjected to quantitative polymerase chain reaction for iron-metabolismrelated genes and protein expression(Western blotting analysis) for ferroportin.As a control,normal liver tissue was obtained from 18 living donors of liver transplantation.Serum hepcidin level was measured by sensitive liquid chromatography/electrospray ionization tandem mass spectrometry.RESULTS:Iron overload is associated with liver damage by increasing oxidative stress and hepatitis C virus(HCV)is reported to induce iron accumulation in hepatocytes in vivo.Conversely,iron administration suppresses HCV replication in vitro.Therefore,the association between HCV infection and iron metabolism remains unclear.Compared with controls,patients had significantly higher gene expression for transferrin,iron-regulatoryproteins 1 and 2,divalent metal transporter 1,and ferroportin,but similar for transferrin receptors 1 and2,and hepcidin.When the expression profiles were compared between sustained virological response(SVR)and non-SVR patients,the former showed significantly lower transcription and protein expression of hepcidin and ferroportin.Expression of hepcidin-regulating genes,BMPR1,BMPR2,and hemojuvelin,was significantly increased,whereas BMP2 was decreased in HCV-infected liver.BMPR2 and hemojuvelin expression was significantly lower in the SVR than non-SVR group.HCV infection affects the expression of iron-metabolism-related genes,leading to iron accumulation in hepatocytes.CONCLUSION:Decreased expression of hepcidin and ferroportin in SVR patients indicates the importance of hepatocytic iron retention for viral response during pegylated-interferon plus ribavirin treatment. | Motoyuki Kohjima Tsuyoshi Yoshimoto Munechika Enjoji Nobuyoshi Fukushima Kunitaka Fukuizumi Tsukasa Nakamura Miho Kurokawa Nao Fujimori Yusuke Sasaki Yasushi Shimonaka Yusuke Murata Susumu Koyama Ken Kawabe Kazuhiro Haraguchi Yorinobu Sumida Naohiko Harada Masaki Kato Kazuhiro Kotoh Makoto Nakamuta | 2015 | World Journal of Gastroenterology2015，21，11： | 4 |
| 2 | PCR-based method for identifying the S-genotypes of Asian pear cultivars显示文摘 | Ishimizu T Inoue K Shimonaka M | 1999 | Theoretical and Applied Genetics1999，98，： | 1 |
| 3 | PCR based method for identifying the S-genotypes of Japanese pear cuhivars显示文摘 | Shimizu T Inoue K Shimonaka M | 1999 | Theor Appl Genet1999，98，： | 1 |
| 4 | Molecular cloning of the cDNAs encoding a novel insulin-like growth factorbinding protein from rat and human显示文摘 | Shimasaki S Uchiyama F Shimonaka M | 1990 | Mol Endocrinol1990，4，10： | 1 |
| 5 | Hepcidin as well as TNFα are significant predictors of arterial stiffness in patients on maintenance hemodialysis显示文摘 | Kuragano T Itoh K Shimonaka Y | | 0，，8： | 1 |
| 6 | Determinants of hepcidin in patients on maintenance hemodialysis:role of inflammation显示文摘 | Kuragano T Shimonaka Y Kida A | | 0，，6： | 1 |
| 7 | Substance P markedly potentiates the antinocieeptive effects of morphine sulfate administered at the spinal level 显示文摘 | Kream RM Kato T Shimonaka H | 1993 | Proc Natl Acad Sci USA1993，90，8： | 1 |
| 8 | Spinal antinociceptive action of Na^+ - K^+ pump inhibitor ouabain and its interaction with morphine and lidocaine in rats显示文摘 | ZENG W DOHI S SHIMONAKA H | 1999 | Anesthesiology1999，90，2： | 1 |
| 9 | Spinal antinociceptive action of Na^+-K^+ pump inhibitor ouabain and its interaction with morphine and lidocaine in rats 显示文摘 | Zeng W Dohi S Shimonaka H | 1999 | Anesthesiology1999，90，2： | 1 |
| 10 | Determinants of hepcidin in patients on maintenance hemodialysis:role of inflammation显示文摘 | Kuragano T Shimonaka Y Kida A | 2010 | Am J Nephrol2010，31，： | 1 |
| 11 | Hepcidin as well as TNF-a are significant predictors of arterial stiffness in patients on maintenance hemodialysis 显示文摘 | KURAGANO T ITOH K SHIMONAKA Y | 2011 | Ncphrol Dial Transplant2011，26，8： | 1 |
| 12 | PCR-based method for identifying the S-genotypes of Japanese pear cultivars显示文摘 | Ishimizu T Inoue K Shimonaka M | 1999 | Theoretical and Applied Genetics1999，98，： | 1 |
| 13 | Substance P markedly potentiates the antinociceptive effects of morphine sulfate administered at the spinal level显示文摘 | Kream RM Kato T Shimonaka H | 1993 | Proc Natl Acad Sci USA1993，90，8： | 1 |
| 14 | Substance P markedly potentiates the antinociceptive effects of morphine sulfate administered at the spinal level显示文摘 | Kream RM Kato T Shimonaka H | | 0，，： | 1 |
| 15 | PCR based method for identifying the S-genotypes of Japanese pear cultivars显示文摘 | Ishimizu T Inoue K Shimonaka M | 1999 | Theoretical and Applied Genetics1999，98，： | 1 |
| 16 | PCR-based method for identifying the S-genotypes of Japanese pear cultivars显示文摘 | T. Ishimizu K. Inoue M. Shimonaka T. Saito O. Terai S. Norioka | 1999 | TAG Theoretical and Applied Genetics (-)1999，，6： | 1 |
| 17 | Hepcidin as well as TNF-alpha are significant predictors of arterial stiffness in patients on maintenance hemodialysis 显示文摘 | Kuragano T Itoh K Shimonaka Y | 2011 | Nephrol Dial Transplant2011，26，： | 1 |
| 18 | Rapl translates chemo- kine signals to integrin activation, cell polarization, and motility across vascular endothelium under flow 显示文摘 | Shimonaka M Katagiri K Nakayama T | 2003 | J Cell Biol2003，161，2： | 1 |
| 19 | Spinal antinociceptive action of Na+-K+ pump inhibitor ouabain and its interaction with morphine and lidocaine in rats显示文摘 | Zeng W Dohi S Shimonaka H | 1999 | Anesthesiology1999，90，2： | 1 |
| 20 | PCR-based method for identifying the S genotypes of Japanese pear eultivars 显示文摘 | Ishimizu T Inoue K Shimonaka M | 1999 | Theoretical and Applied Genetics1999，98，： | 1 |
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