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| 1 | Biosorption mechanisms involved in immobilization of soil Pb by Bacillus subtilis DBM in a multi-metal-contaminated soil显示文摘Mechanisms of soil Pb immobilization by Bacillus subtilis DBM, a bacterial strain isolated from a heavy-metal-contaminated soil, were investigated. Adsorption and desorption experiments with living bacterial cells as well as dead cells revealed that both extracellular adsorption and intracellular accumulation were involved in the Pb2+removal from the liquid phase. Of the sequestered Pb(II), 8.5% was held by physical entrapment within the cell wall, 43.3% was held by ion-exchange, 9.7% was complexed with cell surface functional groups or precipitated on the cell surface, and 38.5% was intracellularly accumulated.Complexation of Pb2+with carboxyl, hydroxyl, carbonyl, amido, and phosphate groups was demonstrated by Fourier transform infrared spectroscopic analysis. Precipitates of Pb5(PO4)3OH, Pb5(PO4)3Cl and Pb10(PO4)6(OH)2that formed on the cell surface during the biosorption process were identified by X-ray diffraction analysis. Transmission electron microscopy–energy dispersive spectroscopic analysis confirmed the presence of the Pb(II)precipitates and that Pb(II) could be sequestered both extracellularly and intracellularly.Incubation with B. subtilis DBM significantly decreased the amount of the weak-acid-soluble Pb fraction in a heavy-metal-contaminated soil, resulting in a reduction in Pb bioavailability, but increased the amount of its organic-matter-bound fraction by 71%. The ability of B.subtilis DBM to reduce the bioavailability of soil Pb makes it potentially useful for bacteria-assisted phytostabilization of multi-heavy-metal-contaminated soil. | Jun Bai Xiuhong Yang Ruiying Du Yanmei Chen Shizhong Wang Rongliang Qiu | 2014 | Journal of Environmental Sciences2014，26，10： | 22 |
| 2 | Genome analysis of Taraxacum kok-saghyz Rodin provides new insights into rubber biosynthesis显示文摘The Russian dandelion Taraxacum kok-saghyz Rodin(TKS), a member of the Composite family and a potential alternative source of natural rubber(NR) and inulin, is an ideal model system for studying rubber biosynthesis. Here we present the draft genome of TKS, the first assembled NR-producing weed plant. The draft TKS genome assembly has a length of 1.29 Gb, containing 46 731 predicted protein-coding genes and68.56% repeats, in which the LTR-RT elements predominantly contribute to the genome enlargement. We analyzed the heterozygous regions/genes, suggesting its possible involvement in inbreeding depression.Through comparative studies between rubber-producing and non-rubber-producing plants, we found that enzymes of the mevalonate(MVA) pathway and rubber elongation might be critical for rubber biosynthesis, and several key isoforms have been isolated and shown to be predominantly expressed in the latex, indicating their crucial functions in rubber biosynthesis. Moreover, for two important families in rubber elongation, the CPT/CPTL and REF/SRPP families, diverse evolutionary tracks have been revealed. These results provide valuable resources and new insights into the mechanism of NR biosynthesis,and facilitate the development of alternative NR-producing crops. | Tao Lin Xia Xu Jue Ruan Shizhong Liu Shigang Wu Xiujuan Shao Xiaobo Wang Lin Gan Bi Qin Yushuang Yang Zhukuan Cheng Suhua Yang Zhonghua Zhang Guosheng Xiong Sanwen Huang Hong Yu Jiayang Li | 2018 | National Science Review2018，5，1： | 22 |
| 3 | Climate and litter C/N ratio constrain soil organic carbon accumulation显示文摘Soil organic carbon(SOC) plays critical roles in stabilizing atmospheric CO2 concentration, but the mechanistic controls on the amount and distribution of SOC on global scales are not well understood. In turn, this has hampered the ability to model global C budgets and to find measures to mitigate climate change. Here, based on the data from a large field survey campaign with 2600 plots across China’s forest ecosystems and a global collection of published data from forested land, we find that a low litter carbon-to-nitrogen ratio(C/N) and high wetness index(P/PET, precipitation-to-potentialevapotranspiration ratio) are the two factors that promote SOC accumulation, with only minor contributions of litter quantity and soil texture. The field survey data demonstrated that high plant diversity decreased litter C/N and thus indirectly promoted SOC accumulation by increasing the litter quality. We conclude that any changes in plant-community composition, plant-species richness and environmental factors that can reduce the litter C/N ratio, or climatic changes that increase wetness index, may promote SOC accumulation. The study provides a guideline for modeling the carbon cycle of various ecosystem scales and formulates the principle for land-based actions for mitigating the rising atmospheric CO2 concentration. | Guoyi Zhou Shan Xu Philippe Ciais Stefano Manzoni Jingyun Fang Guirui Yu Xuli Tang Ping Zhou Wantong Wang Junhua Yan Gengxu Wang Keping Ma Shenggong Li Sheng Du Shijie Han Youxin Ma Deqiang Zhang Juxiu Liu Shizhong Liu Guowei Chu Qianmei Zhang Yuelin Li Wenjuan Huang Hai Ren Xiankai Lu Xiuzhi Chen | 2019 | National Science Review2019，6，4： | 21 |
| 4 | Effect of long term aging on microstructure and stress rupture properties of a nickel based single crystal superalloy | Zhenxue Shi Jiarong Li Shizhong Liu | 2012 | Progress in Natural Science:Materials International2012，22，5： | 14 |
| 5 | 无源非线性延迟锁定保护电路的分析显示文摘本文提出一种简单的无源非线性延迟锁定保护电路。它除具有由复杂的数字逻辑电路构成的计数式延迟锁定保护电路的功能外,对实际工程上常用的rc低通电路相关处理情况,还优于后者。本文分析了无源非线性延迟锁定保护电路的性能,给出了理想积分和rc低通电路相关处理两种情况下的锁定保持时间的均值和方差。 | Yang Shizhong Wu Yongxiang Tang Chaowei Fei Huaipu(Chongqing University,Chongqing 630044) | 1996 | 电子学报1996，24，4： | 10 |
| 6 | Solidification Simulation of Investment Castings of Single Crystal Hollow Turbine Blade显示文摘The three-dimensional solidification simulation of the investment castings of single crystal hollow turbine blade at the withdrawal rates of 2 mm/min, 4.5 mm/min and 7 mm/min has been performed with the finite element thermal analysis. The calculated results are in accordance with the experimental ones. The results show that with the increase of withdrawal rate the concave curvature of the liquidus isotherm is larger and larger, and the temperature gradients of the blades increase. No effects of withdrawal rate on the distribution of the temperature gradients of the starter and helical grain selector of the blades are observed at withdrawal rates of 2 mm/min, 4.5 mm/min and 7 mm/min. The relatively high temperature gradient between 500℃/cm and 1000℃/cm in the starter and helical grain selector is obtained at three withdrawal rates. | Jiarong LI, Shizhong LIU, Hailong YUAN and Zhengang ZHONGNational Key Laboratory of Advanced High Temperature Structural Materials, Beijing Institute of Aeronautical Materials, Beijing 100095, China | 2003 | Journal of Materials Science & Technology2003，19，6： | 9 |
| 7 | Degradation of o-chloronitrobenzene as the sole carbon and nitrogen sources by Pseudomonas putida OCNB-1显示文摘A bacterial strain that utilized o-chloronitrobenzene(o-CNB) as the sole carbon,nitrogen and energy sources was isolated from an activated sludge collected from an industrial waste treatment plant. It was identified as Pseudomonas putida based on its morphology,physiological,and biochemical characteristics with an automatic biometrical system and the 16S rRNA sequence analysis. Microcosm study showed that the biodegradation of o-CNB was optimized at culture medium pH 8.0 and 32°C. At these conditions,the strain degraded 85% of o-CNB at a starting concentration of 1.1 mmol/L in 42 h. o-Chloroaniline was identified as the major metabolite with high performance liquid chromatography(HPLC) and gas chromatography-mass spectrometry(GC-MS) . The study showed that o-CNB degradation by Pseudomonas putida OCNB-1 was initiated by aniline dioxyenase,nitrobenzene reductase and catechol-1,2dioxygenase. | WU Haizhen WEI Chaohai WANG Yaqin HE Qincong LIANG Shizhong | 2009 | Journal of Environmental Sciences2009，21，1： | 9 |
| 8 | Comparison between several seismic inversion methods and their application in mountainous coal fields of western China显示文摘With the objective of establishing the necessary conditions for 3D seismic data from mountainous areas in western China, we compared the application results of wave impedance technology in the lithology and exploration of coal fields. First, we introduce principles and features of three kinds of inversion methods. i.e., Model-Based Inversion, Constrained Sparse Spike Inversion (CSSI) and Geology-Seismic Feature Inversion. Secondly, these inversion methods are contrasted in their application to 3D seismic data from some coalfields in western China. The main information provided by the research includes: improving the vertical resolution of coal deposit strata, inferring lateral variation of the lithology and predicting coal seams and their roof lithology. Finally, the comparison between the three methods shows that the model-based inversion has the higher resolution, while CSSI inversion has better waveform continuity. The geology-seismic feature inversion requires information from a large number of wells and many types of logging curves of good quality. All three methods can meet the requirements of seismic exploration for lithological exploration in coal fields. | XU Yongzhong CHEN Tongjun CHEN Shizhong HUANG Weichuan WU Gang | 2010 | Mining Science and Technology2010，20，4： | 9 |
| 9 | Effects of Rhenium on Creep Rupture Life of a Single Crystal Superalloys显示文摘The slabs of a single crystal superalloy were poured with 2.2% Re, 3.2% Re and 4.2% Re and otherelement contents of the alloys were basicalIy kept no changed. The effects of Re on the ultimatetensile strength, creep rupture life and rafting of a single crystal superalloy were studied. Theresults showed that with increasing Re content the ultimate tensile strength decreased but theplasticity increased at 649℃. and the creep rupture life remarkably increased while the plasticitydecreased at 982℃/248.2 MPa. 1038℃/206.8 MPa and 1070℃/176.8 MPa for the alloy, on themicrostructure aspect, with increasing the creep rupture life of the alloy the rafting was gettingbigger and bigger at the same test condition, and the rafting suppressed creep and increasedcreep rupture life in this single crystal superalloy. | Li, Jiarong Tang, Dingzhong Lao, Riling Liu, Shizhong Wu, Zhongtang
| 1999 | Journal of Materials Science & Technology1999，15，1： | 8 |
| 10 | Enhancing mixing of cohesive particles by baffles in a rotary drum显示文摘 | Zhixing Zhou Jihong Li Jingzhi Zhou Shizhong Li Junxiao Feng | 2016 | Particuology2016，14，2： | 7 |
| 11 | Stability influence factors analysis and construction of a deep beam anchorage structure in roadway roof显示文摘Deep beam anchorage structures based on spatial distribution analysis of the cable prestressed field have been proposed for roadway roof support. Stability and other factors that influence deep beam structures are studied in this paper using mechanical calculations, numerical analysis and field measurements. A mechanical model of deep beam structure subjected to multiple loading is established, including analysis of roof support in the return airway of S1203 working face in the Yuwu coal mine, China. The expression of maximum shear stress in the deep beam structure is deduced according to the stress superposition criterion. It is found that the primary factors affecting deep beam structure stability are deep beam thickness, cable pre-tension and cable spacing. The variation of maximum shear stress distribution and prestressed field diffusion effects according to various factors are analyzed using Matlabòand FLAC3D^(TM) software, and practical support parameters of the S1203 return airway roof are determined.According to the observations of rock pressure, there is no evidence of roof separation, and the maximum values of roof subsidence and convergence of wall rock are 72 and 48 mm, respectively. The results show that the proposed roof support design with a deep beam structure is feasible and achieves effective control of the roadway roof. | Xie Shengrong Gao Mingming Chen Dongdong Sun Yanding Pan Hao Su Hai Lan Shizhong | 2018 | International Journal of Mining Science and Technology2018，28，3： | 7 |
| 12 | Solidification Simulation of Single Crystal Investment Castings显示文摘The three dimensional solidification simulation of the single crystal investment castings at withdrawal rates of 2 mm/min, 4.5 mm/min and 7 mm/min was performed with the finite element thermal analysis method. The calculated results were in accordance with the experimental ones. The results showed that with the increase of withdrawal rate the concave curvature of the liquidus isotherm was bigger and bigger and the temperature gradient of the castings decreased. No effects of withdrawal rate on the distribution of the temperature gradient of the starter and helical grain selector of the castings were observed at withdrawal rates of 2 mm/min, 4.5 mm/min and 7mm/min.The relatively high temperature gradient between 500°C/cm and 1000°C/cm in the starter and helical grain selector was obtained at three withdrawal rates. The study indicates the three dimensional solidification simulation by finite element method is a powerful tool for understanding solidification and predicting defects in single crystal investment castings. | Jiarong LI, Shizhong LIU and Zhengang ZHONGBeijing Institute of Aeronautical Materials, Beijing 100095, China | 2002 | Journal of Materials Science & Technology2002，18，4： | 7 |
| 13 | Genomic selection: A breakthrough technology in rice breeding显示文摘Rice(Oryza sativa)provides a staple food source for more than half the world population.However,the current pace of rice breeding in yield growth is insufficient to meet the food demand of the everincreasing global population.Genomic selection(GS)holds a great potential to accelerate breeding progress and is cost-effective via early selection before phenotypes are measured.Previous simulation and experimental studies have demonstrated the usefulness of GS in rice breeding.However,several affecting factors and limitations require careful consideration when performing GS.In this review,we summarize the major genetics and statistical factors affecting predictive performance as well as current progress in the application of GS to rice breeding.We also highlight effective strategies to increase the predictive ability of various models,including GS models incorporating functional markers,genotype by environment interactions,multiple traits,selection index,and multiple omic data.Finally,we envision that integrating GS with other advanced breeding technologies such as unmanned aerial vehicles and open-source breeding platforms will further improve the efficiency and reduce the cost of breeding. | Yang Xu Kexin Ma Yue Zhao Xin Wang Kai Zhou Guangning Yu Cheng Li Pengcheng Li Zefeng Yang Chenwu Xu Shizhong Xu | 2021 | The Crop Journal2021，9，3： | 7 |
| 14 | Comparison between several multi-parameter seismic inversion methods in identifying plutonic igneous rocks显示文摘With the objective of establishing the necessary conditions for 3-D seismic data from a Permian plutonic oilfield in western China, we compared the technology of several multi-parameter seismic inversion methods in identifying igneous rocks. The most often used inversion methods are Constrained Sparse Spike Inversion (CSSI), Artificial Neural Network Inversion (ANN) and GR Pseudo-impedance Inversion. Through the application of a variety of inversion methods with log curves correction, we obtained relatively high-resolution impedance and velocity sections, effectively identifying the lithology of Permian igneous rocks and inferred lateral variation in the lithology of igneous rocks. By means of a comprehensive comparative study, we arrived at the following conclusions: the CSSI inversion has good waveform continuity, and the ANN inversion has lower resolution than the CSSI inversion. The inversion results show that multi-parameter seismic inversion methods are an effective solution to the identification of igneous rocks. | Yaog Haijun Xu Yongzhong Huang Zhibin Chen Shizhong Yang Zhilin Wu Gang Xiao Zhongyao | 2011 | Mining Science and Technology2011，21，3： | 6 |
| 15 | Chimeric flagellin expressed by Salmonella typhimurium induces an ESAT-6-specific Th1-type immune response and CTL effects following intranasal immunization显示文摘The flagellin component FliC of Salmonella typhimurium is capable of activating the innate immune system via specific interactions with TLR5 and can also act as a carrier of foreign antigen to elicit antigen-specific immune responses.Thus,we constructed an attenuated Salmonella strain SL5928(fliC/esat)expressing chimeric flagellin that contained the ESAT-6 antigen coding sequence of Mycobacterium tuberculosis inserted into the highly variable region of the Salmonella flagellin coding gene fliCi.The chimeric flagellin functioned normally,as demonstrated using a flagella swarming assay and electron microscopy.To analyze the effects of chimeric flagellin,the cell-mediated immune response and cytotoxic T lymphocyte(CTL)effects specific for ESAT-6 antigen were tested after intranasal immunization of mice with flagellated Salmonella SL5928(fliC/esat).The results showed that SL5928(fliC/esat)intranasal immunization can strongly elicit an ESAT-6-specific T helper(Th)1-type immune response in mucosal lymphoid tissues,such as nasopharynx-associated lymph nodes,lung and Peyer’s patches,and a Th1/Th2 response was elicited in spleen and mesenteric lymph nodes.Furthermore,intranasal immunization of SL5928(fliC/esat)produced efficient CTL effects,as demonstrated using a 5-and 6-carboxyfluorescein diacetate succinimidyl ester(CFSE)assay.Thus,our study revealed that Salmonella flagellin acts as a carrier for foreign antigen and triggers strong Th1 and CTL responses during intranasal immunization.Chimeric flagellin is potentially an effective strategy for the development of novel vaccines against tuberculosis in humans and animals. | Hui Zhang Liu Liu Ke Wen Jinlin Huang Shizhong Geng Junsong Shen Zhiming Pan Xinan Jiao | 2011 | Cellular & Molecular Immunology2011，8，6： | 6 |
| 16 | CO_2 efflux from different forest soils and impact factors in Dinghu Mountain, China显示文摘CO2 fluxes from soils and related environmental factors were measured in three forest ecosystems of Dinghu Mountain using static chamber-gas chromatograph technique for one year. The seasonal pattern of CO2 flux, contribution of litter on total CO2 flux and the correlations of CO2 flux with soil temperature and soil water content were examined for each type of forest. The results were given as followings: (1) The seasonal patterns of CO2 flux from soil of the three types of forest were similar, with a higher CO2 flux in rainy season than in dry season. The comparative relations of mean annual CO2 fluxes between the three sites were expressed as:monsoon forest > mixed forest > pine forest. (2) CO2 fluxes from litter decomposition in monsoon forest, mixed forest and pine forest accounted for 24.43%, 41.75% and 29.23% of the corresponding total CO2 fluxes from forest floor, respectively. (3) Significant relationships were found between CO2 fluxes and soil temperatures at 5 cm depth for the three types of forest, which could be best described by exponential equations. The calculated Q10 values based on soil temperature at 5 cm depth ranged from 1.86 to 3.24. More significant relationships were found between CO2 fluxes and soil water content when the annual variation coefficients of soil moisture were higher. | ZHOU Cunyu, ZHOU Guoyi, ZHANG Deqiang, WANG Yinghong & LIU Shizhong Dinghushan Forest Ecosystem Research Station, Chinese Academy of Sciences, Guangzhou 510650, China Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China | 2005 | Science China Earth Sciences2005，48，z1： | 6 |
| 17 | Geology, Geochemistry and Origin of the Hongshan Porphyry-Cryptoexplosive Breccia Type Copper Deposit in Huichang County, Jiangxi Prvince显示文摘The Hongshan porphyry-cryptoexplosive breccia type copper deposit occurs in a metamorphic rockseries of the Mesoproterozoic Zhongcun Group. Orebodies are distributed inside and outside porphyry-cryptoexplosive breccia pipes. The deposit involves five ore-forming types, Le the porphyry type, crytoexplosivebreccia type. contact-zone veinlet-disseminated type, in-pipe fracture-zone filling-replacement type and out-of-pipetracture-zone filling-replacement type, forming an ore-forming system of 'five ore-forming types within a singlerock body' Fluid inclusion and isotope geochemical studies indicate the following: S, Pb, O and Sr were derivedfrom the lower crust, Nd was derived from the continental crust or depleted mantle and rare earth elements (REE)and trace elements have the crustal source characters; fluids consist dominantly of formation water, metamorphic wa-ter and meteoric water with a part of magmatic mater; heat came from porphyry while the latter originated from par-tial melting caused by shear heating in the lower crust and upper mantle. According to its origin the deposit is classi-fied as the hypabyssal and near-surface, meso-and hypothermal copper deposit ussociated with the late Yanshanianporphyry-cryptoexplosive breccia. | ZHOU Jiyuan CUI Bingfang CHEN Shizhong | 1999 | Acta Geologica Sinica(English Edition)1999，73，1： | 5 |
| 18 | Kernel selection with spectral perturbation stability of kernel matrix显示文摘Kernel selection is one of the key issues both in recent research and application of kernel methods.This is usually done by minimizing either an estimate of generalization error or some other related performance measure. Use of notions of stability to estimate the generalization error has attracted much attention in recent years. Unfortunately, the existing notions of stability, proposed to derive the theoretical generalization error bounds, are difficult to be used for kernel selection in practice. It is well known that the kernel matrix contains most of the information needed by kernel methods, and the eigenvalues play an important role in the kernel matrix. Therefore, we aim at introducing a new notion of stability, called the spectral perturbation stability,to study the kernel selection problem. This proposed stability quantifies the spectral perturbation of the kernel matrix with respect to the changes in the training set. We establish the connection between the spectral perturbation stability and the generalization error. By minimizing the derived generalization error bound, we propose a new kernel selection criterion that can guarantee good generalization properties. In our criterion,the perturbation of the eigenvalues of the kernel matrix is efficiently computed by solving the derivative of a newly defined generalized kernel matrix. Both theoretical analysis and experimental results demonstrate that our criterion is sound and effective. | LIU Yong LIAO ShiZhong | 2014 | Science China(Information Sciences)2014，57，11： | 5 |
| 19 | Improvement of catalytic stability for CO_2 reforming of methane by copper promoted Ni-based catalyst derived from layered-double hydroxides显示文摘Copper-promoted nickel-based metal nanoparticles(NPs) with high dispersion and good thermal stability were derived from layered-double hydroxides(LDHs) precursors that were facilely developed by a coprecipitation strategy.The copper-promoted Ni-based metal NPs catalysts were investigated for methane reforming with carbon dioxide to hydrogen and syngas.A series of characterization techniques including XRD,N_2 adsorption and desorption,H_2-TPR,XPS,CO_2-TPD,TEM,TGA and in situ CH_4-TPSR were utilized to determine the structure-function relationship for the obtained catalysts.The copper addition accelerated the catalyst reducibility as well as the methane activation,and made the Ni species form smaller NPs during both preparation and reaction by restricting the aggregation.However,with higher copper loading,the derived catalysts were less active during methane reforming with CO_2 to syngas.It was confirmed that the catalyst with 1 wt%Cu additive gave the higher catalytic activity and remained stable during long time reaction with excellent resistance to coking and to sintering.Furthermore,the mean size of metal NPs changed minimally from 6.6 to 7.9 nm even after 80 h of time on stream at temperature as high as700℃ for this optimized catalyst.Therefore,this high dispersed anti-coking copper-promoted nickel catalyst derived from LDHs precursor could be prospective catalyst candidate for the efficient heterogeneous catalysis of sustainable CO_2 conversion. | Bing Li Zhenxin Xu Fangli Jing Shizhong Luo Ning Wang Wei Chu | 2016 | Journal of Energy Chemistry2016，25，6： | 5 |
| 20 | Oxidation behavior of Mo-Si-B alloys at medium-to-high temperatures显示文摘Continuous exploration of high-temperature structural materials is being driven by the needs of gasturbine engines capable of withstanding the high-temperature environment.Relatively low melting points of currently applied superalloys restrain the further improvement of service tempe ratures.With higher melting tempe ratures above 2000℃,Mo-Si-B alloys are regarded as a new generation of ultrahightemperature structural materials.However,oxidation is a concern for the industrial application of Mo-Si-B alloys.Therefore,an in-depth understanding of the oxidation mechanisms may contribute to solving this issue,whereas relevant reviews about their recent advances are lacking.In the current work,a comprehensively systematic review about the oxidation behaviors of Mo-Si-B alloys is described for this purpose. | Kunming Pan Yanping Yang Shizhong Wei Honghui Wu Zhili Dong Yuan Wu Shuize Wang Laiqi Zhang Junping Lin Xinping Mao | 2021 | Journal of Materials Science & Technology2021，，1： | 5 |
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