| 4 | Potential probiotic and health fostering effect of host gut-derived Enterococcus faecalis on freshwater prawn,Macrobrachium rosenbergii显示文摘The present study investigated the potential probiotic effect of Enterococcus faecalis against a pathogenic microorganism,Vibrio harveyi occurring in the giant freshwater prawn(Macrobrachium rosenbergii).V.harveyi was isolated from the intestine of M.rosenbergii through biochemical tests and PCR-based molecular assays.An in-vitro challenge was conducted by mixing isolated probiotic(5.12 log CFU/g)and V.harveyi(3.09 log CFU/g).Feeds incorporated with E.faecalis(9.02 log CFU/g)were applied to the treatment group during in vivo test in the aquarium and earthen pond(70 m^(2),1 m depth).Pathogenic bacteria were found to be reduced after 8 h(from 5.02 to 3.62 log CFU/g)and 60 days(from 5.44 to 2.15 log CFU/g)of probiotic administration in the in vitro and in vivo test,respectively.The E.faecalis mixed feed also showed higher weight gain(WG%,481.22%±17.71%;SGR%,2.93%±0.18%)and digestive enzymes activities(amylase,1.26±0.08 unit/mg;protease,2.78±0.02 unit/mg)compared to control group(WG%,371.31%±13.35%;SGR%,2.30%±0.32%;amylase,0.7±0.03 unit/mg;protease,1.82±0.02 unit/mg).Furthermore,the probiotic also improved the immune response by augmenting NGH(from 73.33%±0.23%to 74.63%±0.11%)and SGH(from 22.24%±0.06%to 23.13%±0.08%)in the treatment group.Therefore,E.faecalis could be recommended to use against bacterial infections of the M.rosenbergii. | Sharmin Sultana Khushi Mohammad Saifuddin Sumon Mirja Kaizer Ahmmed Md Nazmul Hasan Zilani Fatema Ahmmed Stephen G.Giteru Md Golam Sarower | 2022 | Aquaculture and Fisheries2022，7，1： | 0 |
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