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| 1 | Pre-treatment role of inosine triphosphate pyrophosphatase polymorphism for predicting anemia in Egyptian hepatitis C virus patients显示文摘AIM: To investigate and clarify, for the first time, the role of inosine triphosphate pyrophosphatase (ITPA ) polymorphism in Egyptian chronic hepatitis C virus (HCV) patients.METHODS:The human genomic DNA of all patients was extracted from peripheral blood cells in order to determine the single nucleotide polymorphism (SNP) of ITPA (rs1127354). SNP genotyping was performed by real time polymerase chain reaction (PCR, ABI TaqMan allelic discrimination kit) for 102 treatment-naive Egyptian patients with chronic HCV. All patients had no evidence of cardiovascular or renal diseases. They received a combination treatment of pegylated interferon α (PEG-IFNα) as a weekly subcutaneous dose plus an oral weight-adjusted dose of ribavirin (RBV). The majority received PEG-IFNα2a (70.6%) while 29.4% received PEG-IFNα2b. The planned duration of treatment was 24-48 wk according to the viral kinetics throughout the course of treatment. Pre-treatment liver biopsy was done for each patient for evaluation of fibrosis stage and liver disease activity. The basal viral load level was detected quantitatively by real time PCR while viral load throughout the treatment course was performed qualitatively by COBAS TaqMan assay. RESULTS: Ninety-three patients (91.2%) had ITPA SNP CC genotype and 9 (8.8%) had non-CC genotype (CA and AA). The percentage of hemoglobin (Hb) decline was higher for CC patients than for non-CC patients, particularly at weeks 4 and 8 (P=0.047 and 0.034, respectively). During the first 12 wk of treatment, CC patients had significantly more Hb decline > 3 g/dL than non-CC patients: 64.5% vs 22.2% at weeks 8 and 12, respectively, (P=0.024 and 0.038). Reduction of the amount of the planned RBV dose was significantly higher for CC patients than non-CC patients during the first 12 wk (18% ± 12.1% vs 8.5% ± 10.2%, P=0.021). The percentage of CC patients with RBV dose reduction was significantly greater than that of non-CC patients (77.4% vs 44.4%, P=0.044). Multivariate analysis identified only the percentage of RBV dose as a predictor for Hb decline. Platelet decline was significantly higher in non-CC patients than CC patients at weeks 12, 24 and 48 (P=0.018, 0.009 and 0.026, respectively). CONCLUSION: Rs1127354 ITPA polymorphism plays a decisive role in protecting against treatment-induced anemia and the need for RBV dose reduction in Egyptian HCV patients. | Walaa H Ahmed Norihiro Furusyo Saad Zaky Abeer Sharaf Eldin Hany Aboalam Eiichi Ogawa Masayuki Murata Jun Hayashi | 2013 | World Journal of Gastroenterology2013，19，9： | 2 |
| 2 | Gene expression of 49 kDa apyrase,cytoskeletal proteins,ATPase,ADPase and amino acid contents of Pisum sativum(L.)cells germinated in Euryops arabicus(Steud.ex Jaub.&Spach)water extract显示文摘The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases,cytoskeletal proteins,ATPases,ADPase and amount of amino acid of pea(Pisum sativum L.var.Alaska).Pellets of cytoskeletals proteins(27000 xg)were probed with anti-apyrase antibody,biotinylated anti-rat,actin and alpha and beta-tubulin for Western blotting.ATPase and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate.By 72 hours,the abundance of apyrases,cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds in E.arabicus extracts were sharply increased than those sown in distilled water.All the samples exhibited that the stems had more amount from apyrases,cytoskeletal proteins,amino acids and ATPase and ADPase activities than primary leaves and primary roots that were germinated either on E.arabicus water extract or in distilled water.Based on the enzyme’s capability to hydrolyse nucleotide triphosphate and nucleotide diphosphate as well as the direct association between expression of 49-kDa apyrase and cytoskeletal proteins,E.arabicus water extract had an important effect on plant germinations. | MAHMOUD MOUSTAFA SAAD ALAMRI HOIDA ZAKI NAGLAA LOUTFY TAREK TAHA ALI SHATI MOHAMED ALKAHTANI SAJDA SIDDIQI | 2019 | BIOCELL2019，43，2： | 1 |
| 3 | Temperature-programmed reduction of calcined chromia-coated alumina and silica catalysts: probing chromium (VI)-oxygen species显示文摘 | Mohamed I. Zaki Nasr E. Fouad Geoffrey C. Bond Saad F. Tahir | 1996 | Thermochimica Acta1996，，1： | 1 |
| 4 | Involvement of inflammatory reactions and elevated cell proliferation in the development of bladder cancer in schistosomiasis patients显示文摘 | Rosin MP Saad el Din Zaki S Ward AJ | 1994 | Mutat Res1994，305，2： | 1 |
| 5 | Spectrophotomtric determination of vitamin K3显示文摘 | SAAD S M HASSAN M M Abd El Fattah M T M Zaki | 1975 | Anal Chem1975，275，： | 1 |
| 6 | Spectral sensitivity coefficients (SSCs) of the based materials for photonic devices under optical wavelength and temperature sensing variations in modern optical access networks显示文摘 | Abd El-Naser A Mohammed Ahmed Nabih Zaki Rashed Abd El-Fattah A Saad | | 0，，04： | 1 |
| 7 | Involvement of inflammatory reactions and elevated cell proliferation in the development of bladder cancer in schistosomiasis patients显示文摘 | Rosin MP Saad el Din Zaki S Ward AJ | | 0，，02： | 1 |
| 8 | Dietary effects of A zolla pinnata combined with exogenous digestive enzyme(Digestin^(TM)) on growth and nutrients utilization of freshwater prawn, Macrobrachium rosenbergii(de Man 1879)显示文摘The present study was conducted to evaluate the effect of either individual or combined wheat bran(WB) replacement with Azolla pinnata supplemented with Digestin^(TM) in the diet of freshwater prawn, Macrobrachium rosenbergii Postlarvae(PL) on growth performance, nutrient utilization, chemical body composition and survival(%). Experimental diets were a wheat bran-soybean based diet with no Azolla and Digestin^(TM)(control, T_1), and diets containing 17% Azolla supplemented with Digestin^(TM) 0%(T_2), 1%(T_3), 2%(T_4) and 3%(T 5). Each experimental diet was allocated into three tanks(6 m^3/tank) fed for 12 wks. Each tank was subdivided into three equal pens by nets(2 m^3) and stoked with 84 PL/m^2. The experimental diets were readily consumed by prawns PLs where both high growth and good feed efficiency were achieved for all diets. The results showed that the diets containing A. pinnata supplemented with Digestin? at the level up to 3% have the higher growth and better nutrient utilization than the control diet. No differences were observed for moisture and protein content among the experimental diets. However, the highest protein content was observed on prawns fed on diets T_1 and T 5 respectively, while the lowest value was recorded for T_4 diet. The results also show that prawn PLs fed the diets contain A. pinnata and supplemented with Digestin^(TM) recorded the highest values of body lipid content compared to the control diet. Feed efficiency and economic conversion rate(ECR) values show that economic performance and the cost-effectiveness of the A. pinnata supplemented with up to 3% Digestin^(TM) recorded the highest net return, and therefore it is recommended for prawn, M. rosenbergii PL's. These results are clearly indicating that A. pinnata have a good potential for use in prawn diets at reasonable levels than other conventional diets. | GODA Ashraf SAAD Amal HANAFY Mohamed SHARAWY Zaki EL-HAROUN Ehab | 2018 | Journal of Oceanology and Limnology2018，36，4： | 0 |
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