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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | 显示文摘 | GIBSON R SMITH M D SPARY C J | 2005 | Environ Sci Technol2005，39，： | 1 |
| 3 | Novel G pro- tein- coupled oestrogen receptor GPR30 shows changes in mRNA expression in the rat brain over the oestrous cycle显示文摘 | Spary E J Chapman S E Sinfield J K | 2013 | Neurosignals2013，21，12： | 1 |
| 4 | Behavior of a Small Four- Stroke Engine Using as Fuel Methanol-Gasoline Mixtures 显示文摘 | Arapatsakos C Karkanis A Sparis P | 2003 | SAE Paper2003，32，： | 1 |
| 5 | A novel form of cellular communication among thymicepithelcal cells: intercellular calcium wave propagation显示文摘 | Nihei OK Campas de Carvalho AC Spary DC | 2003 | Am J Cell Physiol2003，285，： | 1 |
| 6 | Correlation of wind speedbetween neighboring measuring stations 显示文摘 | BECHRAKIS D A SPARIS P D | 2004 | IEEE Trans onEnergy Conversion2004，19，2： | 1 |
| 7 | Changes in glutamate transporter expression in mouse forebrain areas following focal ischemia显示文摘 | Ketheeswaranathan P Turner NA Spary E J | 2011 | Brain Res2011，1418，： | 1 |
| 8 | GABA(B) Mediated Regulation of Sympathetic Preganglionic Neurons:Pre-and Postsynaptic Sites of Action显示文摘 | Wang L Bruce G Spary E | | 0，，： | 1 |
| 9 | An estimation of three-way catalyst performance using artificial neural networks during cold start显示文摘 | Botsaris P N Bechrakis D Sparis P D | 2003 | Applied Catalysis A:General2003，243，9： | 1 |
| 10 | Cross-presentation of the oncofetal tumor antigen 5T4 from irradiated prostate cancer cells-A key role for heat-shock protein 70 and receptor CD91 显示文摘 | Salimu J Spary L K A1-Taei S | 2015 | Cancer Immunol Res2015，3，6： | 1 |
| 11 | Changes in glutamate transpoeter expression in mouse forebrain areas folloing focal ischemia显示文摘 | Ketheeswaranathan P Turner NA Spary EJ | 2011 | Brain Res2011，，1418： | 1 |
| 12 | Correlation of wind speed between neighboring measuring stations显示文摘 | BECHRAKIS D A SPARIS P D | 2004 | IEEE Transactions on Energy Conversion2004，19，2： | 1 |
| 13 | Mixtures of Estrogenic Coritaminants in Bile of Fish Exposed to Wastewater Treatment Works Effluents 显示文摘 | Gibson R Smith MD Spary C J | 2005 | Environ Sci Technol2005，39，8： | 1 |
| 14 | Correlation of wind speed between neighboring measuring stations显示文摘 | BECHRAKIS D A SPARIS P D | 2004 | IEEE Transactions on Energy Conversion2004，19，2： | 1 |
| 15 | Wind Speed Prediction U- sing Artificial Neural Networks显示文摘 | BECHRAKIS D A SPARIS P D | 1998 | Wind Engineering1998，22，6： | 1 |
| 16 | Increased expression of AMPA receptor subunits in the nucleus of the solitary tract in the spontaneously hypertensive rat显示文摘 | Saha S Spary E J Maqbod A | 2004 | Brain Res Mol Brain Res2004，121，12： | 1 |
| 17 | Intrapartal resection of the bicornuete uterus for placenta membranacea percreta 显示文摘 | Spari R Kadija S Tadic J | 2007 | Srp Arh Celok Lek2007，135，2： | 1 |
| 18 | Accounting for differences in estrogenic responses in rainbow trout (Oncorhynchus mykiss : Salmonidae ) and roach ( Rutilus rutilus : Cyprinidae ) exposed to effluents from wastewater treatment works 显示文摘 | TYLER C R SPARY C GIBSON R | 2005 | Environmental Science & Technology2005，39，8： | 1 |
| 19 | Correlation of wind speed between neighboring measuring stations显示文摘 | BECHRAKIS D A SPARIS P D | | 0，，02： | 1 |
| 20 | Multiorgan dysfunction in a gravid woman with placental abruption and disseminated intravascular coagula- tion显示文摘 | Kezi A Spari R Stojimirovi B | 2007 | Srp Arh Celok Lek2007，135，78： | 1 |
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