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72篇 您的检索式:作者名="Ryo H"
    题名 作者 年代 出处 被引量
1Molecular diagnostics in a teacup:Non-Instrumented Nucleic Acid Amplification(NINA) for rapid,low cost detection of Salmonella enterica显示文摘We report on the use of a novel non-instrumented platform to enable a Loop Mediated isothermal Amplification(LAMP) based assay for Salmonella enterica.Heat energy is provided by addition of a small amount(<150 g) of boiling water,and the reaction temperature is regulated by storing latent energy at the melting temperature of a lipid-based engineered phase change material.Endpoint classification of the reaction is achieved without opening the reaction tube by observing the fluorescence of sequence-specific FRET-based assimilating probes with a simple handheld fluorometer.At or above 22℃ ambient temperature the non-instrumented devices could maintain reactions above a threshold temperature of 61℃ for over 90 min-significantly longer than the 60 min reaction time.Using the simple format,detection limits were less than 20 genome copies for reactions run at ambient temperatures ranging from 8 to 36℃.When used with a pre-enrichment step and non-instrumented DNA extraction device,trace contaminations of Salmonella in milk close to 1 CFU/mL could be reliably detected.These findings illustrate that the non-instrumented amplification approach is a simple,viable,low-cost alternative for field-based food and agricultural diagnostics or clinical applications in developing countries.KUBOTA Ryo LABARRE Paul WEIGL Bernhard H LI Yong HAYDOCK Paul JENKINS Daniel M 2013Chinese Science Bulletin2013,58,10:4
2Similarity among the Drosophila (6-4)photolyase, a human photolyase homolog,and the DNA photolyase-blue-light photoreceptor family显示文摘Todo T Ryo H Yamamoto K 1996Science1996,272,5258:1
3Activation of an ER-body-localized β-Glucosidase via a cytosolic binding partner in damaged tissues of Arabidopsis thaliana 显示文摘Atsushi J N Ryo M Ikuko H N 2005Plant & cell Physiology2005,46,7:1
4显示文摘RYO S TAMIO H 2006J Am Chem Soc2006,128,:1
5Stable suppression of tumorigenicity by Pin1-targeted RNA interference in prostate cancer显示文摘Ryo A Uemura H Ishiguro H 2005Clin Cancer Res2005,11,20:1
6Water- soluble extracts from Angelica acutiloba kitagawa enhance hematopoiesis by activating immature erythroid cells in mice with 5 - fluorouracil - induced anemia 显示文摘Ryo H Fumihide T Shinji F 2004Experimental Hematology2004,32,:1
7Effects of pulsed ultrasound on development and glucose uptake of preimplantation mouse embryos 显示文摘Ryo E Shiotsu H Takai Y 2001Ultrasound Med Biol2001,27,7:1
8Control and analysis of the modular multilevelascade converterased on double-starhop-per-cells(MMCC-DSCC)显示文摘Makoto H Ryo M Hirofumi A 2011IEEE Trans on Power Electronics2011,26,6:1
9Magnetic and Neutron Diffraction Study on Quaternary Oxides MTe Mo O6(M=Mn and Zn)显示文摘Yoshihiro D Ryo S Yukio H 2009Journal of Physics:Condensed Matter2009,21,46:1
10A new model for predicting time coursetoxicity of heavy metals based on Biotic Ligand Model(BLM)显示文摘AYUMI H RYO S 2010Comparative Biochemistry and Physiology Part C Toxicology& Pharmacology2010,15,1:1
11Determination of biguanide groups in polyhexamethylene biguanide hydrochloride by titrimetrie methods 显示文摘TOSHIAKI H YASUKO N RYO K 2003Analytical Sciences2003,19,:1
12Thyroid gland tumor diagnosis at US elastography显示文摘Andrej L Tatsuya H Ryo A 2005Radiology2005,237,1:1
13Possible involvement of oxidatire stress in salivary gland of patients with Sjogren' s syndrome 显示文摘Ryo K Yamada H Nakagawa Y Tai Y Obara K Inoue H 2006Pathobiology2006,73,5:1
14Stable suppression of tu- morigenicity by Pinl-targeted RNA interference in prostate cancer 显示文摘Ryo A Uemura H Ishiguro H 2005Clin Cancer Res2005,11,20:1
15A prolactin-releasing peptide in the brain 显示文摘Shuji H Yugo H Ryo F 1998Nature1998,393,6682:1
16Singularty-spreading phase unwrapping显示文摘Ryo Y Akria H 2007IEEE Transactions on Geoscience and Remote Sensing2007,45,10:1
17Bone metastasis of primary lung cancer显示文摘Ryo H Sakai H Yoneda S 1998Nihon Kokyuki Gakkai Zasshi1998,36,4:1
18Thyroid gland tumor diagnosis at US elastography显示文摘Andrej L Tatsuya H Ryo A 2005Radiology2005,237,:1
19Prompt diagnosis of ectopic pregnancy with the huulan chorionic gonadotropin test using exuded extract from endometrial curettings显示文摘Ryo E Yokoyama S Koizumi H 2006Fertil Steri12006,85,:1
20Transcatheter closure of secundum atrial septal defect显示文摘Shimpo H Hojo R Ryo M 2013Gen Thorac Cardiovasc Surg2013,61,11:1
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