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| 1 | Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein显示文摘为了发展,一支疫苗,它能同时阻止疾病在鱼由各种各样的病原的细菌引起了,我们试着从不同细菌的病原体发现保存的外部膜蛋白质(OMP ) 抗原。在这研究, 747 bp 的 OMP 碎片(作为 Omp-G 说出),它高度从 NCBI 数据库在七个 Aeromonas OMP 序列被保存,被 PCR 与设计特定的教材从一 Aeromonas sobria 紧张( B10 )和二 Aeromonas hydrophila 紧张( B27 和 B33 )放大。顺序被克隆进 pGEX-2T (6 敶 ? 慣据牥爭汥瑡摥洠剩 ?? 敷敲搠瑥 ' 虘 b 汢? 湵敤 ? 慥档漠 ? 整瑳挠湯楤楴湯 ? 椠摮捩瑡湩 ? 桴瑡洠剩 ?? 敷敲攠瑸敲敭祬猠慴汢? 湡 ? 敲楳瑳湡 ? 潴搠獥牴 ' 讟 N 湯愠摮搠来慲慤楴湯甠摮牥栠牡桳攠癮物湯敭瑮污挠湯楤楴湯吗? | Ruizhang Guan Jing Xiong Wenshu Huang Songlin Guo | 2011 | Acta Biochimica et Biophysica Sinica2011，43，1： | 12 |
| 2 | Arabidopsis Indole Synthase, a Homolog of Tryptophan Synthase Alpha, is an Enzyme Involved in the Trp-independent Indole-containing Metabolite Biosynthesis显示文摘The plant tryptophan (Trp) biosynthetic pathway produces many secondary metabolites with diverse functions. Indole- 3-acetic acid (IAA), proposed as a derivative from Trp or its precursors, plays an essential role in plant growth and development. Although the Trp-dependant and Trp-independent IAA biosynthetic pathways have been proposed, the enzymes, reactions and regulatory mechanisms are largely unknown. In Arabidopsis, indole-3-glycerol phosphate (IGP) is suggested to serve as a branchpoint component in the Trp-independent IAA biosynthesis. To address whether other enzymes in addition to Trp synthase α (TSA1) catalyze IGP cleavage, we identified and characterized an indole synthase (INS) gene, a homolog of TSA1 in Arabidopsis. INS exhibits different subcellular localization from TSA1 owing to the lack of chloroplast transit peptide (cTP). In silico data show that the expression levels of INS and TSA1 in all examined organs are quite different. Histochemical staining of INS promoter-GUS transgenic lines indicates that INS is expressed in vascular tissue of cotyledons, hypocotyls, roots and rosette leaves as well as in flowers and siliques. INS is capable of complementing the Trp auxotrophy of Escherichia coli trpA strain, which is defective in Trp synthesis due to the deletion of TSA. This implies that INS catalyzes the conversion of IGP to indole and may be involved in the biosynthesis of Trp-independent IAA or other secondary metabolites in Arabidopsis. | RuiZhang Bing Wang Jian Ouyang Jiayang Li YonghongWang | 2008 | Journal of Integrative Plant Biology2008，50，9： | 4 |
| 3 | Effects of neferine on Kv4.3 channels expressed in HEK293 cells and ex vivo electrophysiology of rabbit hearts显示文摘目的: Neferine 是从 Nelumbo nucifera (Gaertn ) 的种子胚胎孤立的 isoquinoline 碱,它举办许多生物活动。在这研究,我们在 Kv4.3 隧道上检验了 neferine 的效果,一个主要贡献者到短暂外面的水流(我 到在兔子心的),并且在兔子 hearts.Methods 的前 vivo electrophysiology 上:整个房间的 Kv4.3 水流在用补丁夹钳表示人的心脏的 Kv4.3 隧道的 HEK293 房间被记录技术。兔子的动脉地酒的楔离开了室( LV )被准备,并且 transmembrane 行动潜力同时和 transmural electrocardiography ( ECG )与漂浮的 microelectrodes 从 epicardial ( Epi )和 endocardial ( Endo )地点被记录 .Results : Neferine ( 0.1-100 μ ; mol/L ) dose-dependently 并且 reversibly 禁止的 Kv4.3 水流( IC 50价值是 8.437 μ ; mol/L ,并且在 100 μ 的最大的抑制; mol/L 是44.12%)。Neferine (10 μ mol/L ) 引起了 Kv4.3 水流,和不变的 inactivation 曲线的否定移动的不变的激活曲线的积极移动。而且, neferine (10 μ mol/L ) 加速了 inactivation 然而并非 Kv4.3 水流的激活,并且显著地从 inactivation 减缓了 Kv4.3 水流的恢复。Kv4.3 水流的导致 Neferine 的堵住是频率依赖者。在兔子 LV 的动脉地酒的楔, neferine ( 1 , 3 ,和 10 μ ; mol/L ) dose-dependently 在 Epi 和 Endo 地点延长了 QT 间隔和行动潜力持续时间( APD ),并且在 Epi sites.Conclusion 引起了 APD 10的戏剧的增加: Neferine 由堵住开的状态并且使州的隧道失去活性多半禁止 Kv4.3 隧道,它在兔子心的 Epi 地点贡献 APD 10的 导致neferine 的戏剧的增加。 | Chen WANG Yu-fang CHEN Xiao-qing QUAN Huan WANG RuiZHANG Jun-hua XIAO Jia-ling WANG Cun-tai ZHANG Ji-zhou XIANG Qiang TANG | 2015 | Acta Pharmacologica Sinica2015，36，12： | 4 |
| 4 | The steady-state level of CDK4 protein ms regulated by antagonistic actions between PAQR4 and SKP2 and involved in tumorigenesis显示文摘 | Lin Wang RuiZhang Xue You Huanhuan Zhang Siying Wei Tingting Cheng Qianqian Gao ZhenzhenWang Yan Chen | 2017 | Journal of Molecular Cell Biology2017，9，5： | 4 |
| 5 | Use of Azotobacter sp.as an indicator to detect the toxicity of heavy metals in soils显示文摘A physiological strain of microorganism - Azotobacter sp. has been adopted as an indicator to detect the toxicity of heavy metals in soils. The concentration of heavy metals to which Azotobacter sp. was behaving initially to have the resistance to heavy metals is defined as the critical poisoning concentration. The method of physiological threshold adopted can have a quantitative determination with reproducible results. The determined critical poisoning concentration is basically consistent with the results of heavy metals and arsenic toxicities to the bacteria reported recently in literatures. Total 9 typical soils, including 6 zonal soils and 3 purple soils, in the whole country were determined for the toxicities of 5 heavy metals and arsenic to Azotobacter sp. that resulted in 48 critical poisoning concentrations. | Liao Ruizhang, Shen Qiuqin, Jin Lizhi, Shen Shuling, Qian Houyin and Guan ZhenshengInstitute of Soils and Fertilizers,Chinese Academy of Agricultural Sciences,Beijing,China | 1990 | Journal of Environmental Sciences1990，2，3： | 2 |
| 6 | Generation of insulin‐producing cells from PDX‐1 gene‐modified human mesenchymal stem cells显示文摘 | YanhuaLi RuiZhang HaifaQiao HepingZhang YunfangWang HongfengYuan QinbinLiu DaqingLiu LinChen XuetaoPei | 2007 | J Cell Physiol2007，，1： | 2 |
| 7 | Preparation of High‐Performance Conductive Polymer Fibers through Morphological Control of Networks Formed by Nanofillers显示文摘 | HuaDeng TetyanaSkipa EmilianoBilotti RuiZhang DirkLellinger LucaMezzo QiangFu IngoAlig TonPeijs | 2010 | Adv Funct Mater2010，，9： | 1 |
| 8 | Framing AnalysisoftheChina MediaCoverageonForeignBrandsNegativeInformation:Reflection of National Interest and Self -consciousness显示文摘 | JinpingLiu YiZhang Lin Lan RuiZhang &FeifeiLiu | 2013 | InformationTechnologyJournal2013，12，10： | 1 |
| 9 | Establishment of Elymus natans improves soil quality of a heavily degraded alpine meadow in Qinghai-Tibetan Plateau, China显示文摘 | Ruizhang Feng Ruijun Long Zhanhuan Shang Yushou Ma Shikui Dong Yanlong Wang | 2010 | Plant and Soil (-)2010，，1： | 1 |
| 10 | Dirichlet process mixture model for document clustering with feature partition 显示文摘 | Huang Ruizhang Yu Guan Wang Zhaojun | 2013 | IEEE Trans on Knowledge and Data Engineering2013，25，8： | 1 |
| 11 | Feeding ecology of the signal cray-fish Pacifaslacus leniusculus in a British lowland river 显示文摘 | GU AN Ruizhang WILKS P R | 1998 | Aqua-culture1998，169，3： | 1 |
| 12 | SHS TiB2-based multiphase ceramics and composites 显示文摘 | YUAN Ruizhang FU Zhengyi WANG Weimin | 2001 | Int J SHS2001，10，4： | 1 |
| 13 | Centralizedand distributed resoureealloeationin relaysystem 显示文摘 | Yin Ruizhang Yu Yu Zhang Guanding | 2010 | Journalof Zhejiang University SeieneeC2010，11，6： | 1 |
| 14 | Fundamental study on SHS preparation of TiB2-AI composites 显示文摘 | FU Zhengyi YUAN Ruizhang MUNIR Z A | 1992 | Int J SHS1992，1，1： | 1 |
| 15 | Phase-Locking of a Gunn Oscillator at 3mm Waveband Using High Temperature Superconducting Josephson Junction Harmonic Mixer显示文摘A Gunn oscillator at 3mm waveband has been phase-locked to a synthesizer local oscillator using a high T_(c)YBa_(2)Cu_(3)O_(7-δ) Josephson junction operated at 77K as the harmonic mixer,with harmonic numbers up to 52.The observed spectrum of the phase-locked output at the intermediate frequency(IF)shows clearly that the quality of the phase-locking is very good.To drive the analogue phase locking loop successfully,it is essential to have IF output and signal-to-noise ratio both as high as possible. | WANG Huabing XU Weiwei CHENG Qiheng JI Zhengming GAO Ruizhang WU Peiheng XU Zhicai | 1995 | Chinese Physics Letters1995，12，4： | 1 |
| 16 | Molecular cloning and characterization analysis of immunoglobulin M heavy chain gene in European eel ( Anguilla anguilla )显示文摘 | Jianjun Feng Ruizhang Guan Peng Lin Songlin Guo | 2008 | Veterinary Immunology and Immunopathology2008，，1： | 1 |
| 17 | Characteristics of denaturized chondroitin sulfate from sturgeon显示文摘 | ZHENG Jiang TIAN Yonghua GUAN Ruizhang | 2008 | J Biotechnol2008，136，： | 1 |
| 18 | Study on the toxicity of acid rain to microbiota in soils显示文摘With simulated acid rain and acidification, the soils from both Nanning and Liuzhou municipalities, Guangxi Zhuang Nationality Autonomous Region have been studied to determine the counts of azotobacter, bacteria, actinomyces and the activity of urease in soils, and the changes in respiratory intensity of soil so as to identify the toxicity of acidic substances to microbiota and enzyme in such soils. The concept of the critical pH of toxicity has been developed and used to discuss the representation of the concept, the basis on which the soil treated with simulated acid rain can be taken as an object of study, and the criteria for the identification of toxicity. Based on that as mentioned above, it was found that acid rain behaved to have toxicity to the microbiota in soils from Liuzhou other than from Nanning. The findings may be regarded as an objective basis to study the toxicity of acidic substances to microbiota and enzyme in soils in this region. | Liao Ruizhang , Cao Hongfa, Liu Liangui and Gao JinlanInstitue of Soils and Fertilizers,Chinese academy of Agricultural Sciences,Beijing 100081,China.Institue of Ecology,Chinese Academy of Environmental Sciences,Beijing 100012,China | 1991 | Journal of Environmental Sciences1991，3，2： | 0 |
| 19 | GP×1 knockdown suppresses chondrogenic differentiation of ATDC5 cells through induction of reductive stress显示文摘谷胱甘肽 peroxidase (GPx1 ) 1 是包含蛋白质的硒(Se ) 并且在软骨形成被导致。GPx1 消除反应的氧种类(ROS ) ,它为 chondrogenic 正式就职被要求。在软骨和包含的氧化还原作用机制的 GPx1 的生理的性质不被知道。ATDC5 房间的 chondrogenic 区别上的 GPx1 的效果通过短发卡被检验调停 RNA 的基因 silencing。结果表明了那 GPx1 决定区域 Y 盒子的性的击倒的损害基因表示 9,骨胶原 II (关口 II ) ,和 aggrecan。GPx1 击倒压制了软骨 glycosaminoglycans 的累积(作呕) 并且 chondrocyte 的增长。GPx1 击倒的也导致的房间 apoptosis。然而,向外长的氧化应力的房间敏感没在 GPx1 以后被增加击倒。出人意料地, GPx1 击倒不仅 ROS 的增加的生产描绘而且引起了减少的应力的导致的氧化应力由谷胱甘肽(GSH ) 的举起显示了 /oxidized GSH (GSSG ) 比率。而且,减少的 GPx1 击倒调停和氧化应力能被氧化 thiol 代理人 diamide 和包含 thiol 混合物 N-acetylcysteine (NAC ) 分别地反对。而且, NAC 稀释了 GPx1 击倒导致房间 apoptosis,当 diamide 阻止了 GPx1 击倒压制 chondrocyte 增长时。最后, diamide 然而并非 NAC 能救 GPx1 击倒调停损害 chondrogenic 区别。在摘要, GPx1 通过细胞内部的 GSH/GSSG 比率,而非抗氧化剂酶的调整主要为在 ATDC5 房间感应的 chondrogenic 是必要的除去 ROS。另外, GPx1 击倒导致损害了 chondrogenesis 可以由于 Se 参予地方性的 osteoarthropathy 的致病缺乏。这些观察在软骨退化期间为治疗学的目标的发展提供新奇卓见。 | Jidong Yan Yuanxu Guo Yao Fei RuiZhang Yan Han Shemin Lu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，2： | 0 |
| 20 | Reversing of multidrug resistance in leukemia cell Line with gene transfer K562/MDR by traditional chinese medicine combining with west medicine and the correlative mechanism显示文摘 | RuiZhang JinyaoZhao LiKong | 2004 | 中国组织化学与细胞化学杂志2004，13，3： | 0 |
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