5篇
您的检索式：作者名="Ruixing Hou"
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| 1 | Quercetin promotes human epidermal stem cell proliferation through the estrogen receptor/β-catenin/c-Myc/cyclin A2 signaling pathway显示文摘Skin epidermal stem cells(EpSCs)play an important role in wound healing.Quercetin is a phytoestrogen reported to accelerate skin wound healing,but its effect on EpSCs is unknown.In this study,we investigated the effect of quercetin on human EpSC proliferation and explored the underlying mechanisms.We found that quercetin at 0.1~1μM significantly promoted EpSC proliferation and increased the number of cells in S phase.The pro-proliferative effect of quercetin on EpSCs was confirmed in cultured human skin tissue.Mechanistic studies showed that quercetin significantly upregulated the expressions ofβ-catenin,c-Myc,and cyclins A2 and E1.Inhibitor forβ-catenin or c-Myc significantly inhibited quercetin-induced EpSC proliferation.Theβ-catenin inhibitor XAV-939 suppressed quercetin-induced expressions ofβ-catenin,c-Myc,and cyclins A2 and E1.The c-Myc inhibitor 10058-F4 inhibited the upregulation of c-Myc and cyclin A2 by quercetin.Pretreatment of EpSCs with estrogen receptor(ER)antagonist ICI182780,but not the G protein-coupled ER1 antagonist G15,reversed quercetin-induced cell proliferation and upregulation ofβ-catenin,c-Myc,and cyclin A2.Collectively,these results indicate that quercetin promotes EpSC proliferation through ER-mediated activation ofβ-catenin/c-Myc/cyclinA2 signaling pathway and ER-independent upregulation of cyclin E1 and that quercetin may accelerate skin wound healing through promoting EpSC proliferation.As EpSCs are used not only in clinic to treat skin wounds but also as seed cells in skin tissue engineering,quercetin is a useful reagent to expand EpSCs for basic research,skin wound treatment,and skin tissue engineering. | Zhaodong Wang Guangliang Zhang Yingying Le Jihui Ju Ping Zhang Dapeng Wan Qiang Zhao Guangzhe Jin Hao Su Jinwei Liu Jiaxuan Feng Yi Fu Ruixing Hou | 2020 | Acta Biochimica et Biophysica Sinica2020，52，10： | 3 |
| 2 | Effects of 11 years of conservation tillage on soil organic matter fractions in wheat monoculture in Loess Plateau of China显示文摘 | Haiqing Chen Ruixing Hou Yuanshi Gong Hongwen Li Mingsheng Fan Yakov Kuzyakov | 2009 | Soil & Tillage Research2009，，1： | 2 |
| 3 | Effects of tillage and residue management on soil organic carbon and total nitrogen in the North China Plain显示文摘 | HOU Ruixing OUYANG Zhu Li Yunsheng | | 0，，： | 1 |
| 4 | Ulnar artery distal cutaneous descending branch as free flap in hand reconstruction显示文摘 | JU Jihui LIU Yuefei HOU Ruixing | 2009 | Injury2009，40，12： | 1 |
| 5 | Angiopoietin-like 4 promotes epidermal stem cell proliferation and migration and contributes to cutaneous wound re-epithelialization显示文摘Proliferation and migration of epidermal stem cells(EpSCs)are essential for epithelialization during skin wound healing.Angiopoietin-like 4(ANGPTL4)has been reported to play an important role in wound healing,but the mechanisms involved are not fully understood.Here,we investigate the contribution of ANGPTL4 to full-thickness wound re-epithelialization and the underlying mechanisms using Angptl4-knockout mice.Immunohistochemical staining reveals that ANGPTL4 is significantly upregulated in the basal layer cells of the epidermis around the wound during cutaneous wound healing.ANGPTL4 deficiency impairs wound healing.H&E staining shows that ANGPTL4 deficiency significantly reduces the thickness,length and area of the regenerated epidermis postwounding.Immunohistochemical staining for markers of EpSCs(α6 integrin andβ1 integrin)and cell proliferation(PCNA)shows that the number and proliferation of EpSCs in the basal layer of the epidermis are reduced in ANGPTL4-deficient mice.In vitro studies show that ANGPTL4 deficiency impedes EpSC proliferation,causes cell cycle arrest at the G1 phase and reduces the expressions of cyclins D1 and A2,which can be reversed by ANGPTL4 overexpression.ANGPTL4 deletion suppresses EpSC migration,which is also rescued by ANGPTL4 overexpression.Overexpression of ANGPTL4 in EpSCs accelerates cell proliferation and migration.Collectively,our results indicate that ANGPTL4 promotes EpSC proliferation by upregulating cyclins D1 and A2 expressions and accelerating the cell cycle transition from G1 to S phase and that ANGPTL4 promotes skin wound re-epithelialization by stimulating EpSC proliferation and migration.Our study reveals a novel mechanism underlying EpSC activation and re-epithelialization during cutaneous wound healing. | Yuan Yang Chenghao Yu Yingying Le Weijuan Gong Jihui Ju Guangliang Zhang Pengxiang Ji Rui Zuo Zhe Liu Ping Zhang Ruixing Hou Yi Fu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，8： | 0 |
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