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| 1 | Antihepatoma effect of alpha-fetoprotein antisense phosphorothioate oligodeoxyribonucleotides in vitro and in mice显示文摘AIM To evaluate antihepatoma effect ofantisense phosphorothioate oligodeo-xyribonucleotides (S-ODNs) targeted to alpha-fetoprotein (AFP) genes in vitro and in nudemice.METHODS AFP gene expression was examinedby immunocytochemical method or enzyme-linked immunosorbent assay. Effect of S-ODNson SMMC-7721 human hepatoma cell growth invitro was determined using microculturetetrazolium assay. In vivo antitumor activitiesof S-ODNs were monitored by measuring tumorweight differences in treated and control micebearing SMMC-7721 xenografts. Induction of cellapoptosis was evaluated by fluorescence-activated cell sorter (FACS) analysis.RESULTS Antisense S-ODN treatment led toreduced AFP gene expression. Specificantisense S-ODNs, but not control S-ODNs,inhibited the growth of heaptoma cells in vitro.In vivo. only antisense S-ODNs exhibitedobvious antitumor activities. FACS analysisrevealed that the growth inhibition by antisenseS. ODNs was associated with their cell apoptosisinduction.CONCLUSION Antisense S-ODNs targeted toAFP genes inhibit the growth of human hepatomacells and solid hepatoma, which is related totheir cell apoptosis induction. | Xing Wang Wang~1 Jin Hui Yuan~1 Ru Gang Zhang~1 Li Xia Guo~1 Yong Xie~2 Hong Xie~1 ~1Department of Biotherapy,Shanghai Institute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China ~2Department of Biology,Hong Kong University of Science and Technology,ChinaDr.Xing Wang Wang earned Ph.D.from Shanghai Institute of Materia Medical,Chinese Academy of Sciences in 1997.Now a professor at Shanghai Institute of Cell Biology,Chinese Academy of Sciences. | 2001 | World Journal of Gastroenterology2001，7，3： | 21 |
| 2 | Growth-inhibiting effects of taxol on human liver cancer in vitro and in nude mice显示文摘AIM To investigate the effects of taxol onSMMC-7721 human hepatoma and itsmechanisms.METHODS In vitro cell growth was assessedby trypan blue exclusion method.Experimentalhepatoma model was established by seedingSMMC-7721 cells subcutaneously into Balb/c(nu/nu)nude mice.In vivo tumor growth wasdetermined by measurement of tumor diameterwith Vernier calipers.The syntheses of DNA,RNA and protein were analyzed by incorporationof ~3H-thymidine,~3H-uridine and ~3H-leucinerespectively.Using light and electronmicroscopes to observe the morphologicalchanges of cells including mitosis andapoptosis.RESULTS Taxol was effective against SMMC-7721 human hepatoma cell growth in the rangesof 2.5 nmol/L-10 nmol/L with mitotic arrestand apoptosis in vitro.DNA,RNA and proteinsyntheses in cells were also obviouslysuppressed by in vitro treatment of taxol for72 h.Taxol at 2.5 nmol/L reduced ~3H-thymidineuptake to about 34% of the control value(P<0.05).Increasing the dose of taxol to20 nmol/L resulted in a greater decrease in ~3H-thymidine incorporation to 60% of the controlvalue(P<0.01).At a concentration of 20 nmol/L,the ~3H-uridine and ~3H-leucine uptakeswere reduced to 52%(P<0.05)and 63%(P<0.01),respectively.In vivo,taxolsignificantly inhibited SMMC-7721 tumor growthat 10 mg/kg,i.p.,once daily for 10 d.A morethan 90% decrease in tumor volume wasobserved by day 11(P<0.01)similarly withmitotic arrest and cell apoptosis.CONCLUSION Taxol has a marked anticanceractivity in SMMC-7721 human hepatoma both invitro and in nude mice.Its mechanisms might beassociated with mitotic arrest,subsequently,apoptosis of the hepatoma cells.No obvioustoxicity was observed with in vivoadministration of taxoi. | Jin Hui Yuan Ru Ping Zhang Ru Gang Zhang Li Xia Guo Xing Wang Wang Dan Luo Yong Xie Hong Xie | 2000 | World Journal of Gastroenterology2000，6，2： | 18 |
| 3 | Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells显示文摘A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in ail of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences. | ZHANG RU GANG XING WANG WANG JIN HUI YUAN LI XIA GUO HONG XIE (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China) | 2000 | Cell Research2000，10，1： | 8 |
| 4 | Regular Space-like Hypersurfaces in S1^m+1 with Parallel Para-Blaschke Tensors显示文摘In this paper, we give a complete conformal classification of the regular space-like hypersurfaces in the de Sitter Space S_1^(m+1) with parallel para-Blaschke tensors. | Xing Xiao LI Hong Ru SONG | 2017 | Acta Mathematica Sinica,English Series2017，33，10： | 5 |
| 5 | Atomic-scale observation of a two-stage oxidation process in Cu2O显示文摘Cu 2 O nanocrystallines (NC ) 直接被观察由在 situ 高分辨率的传播电子显微镜学。一个二阶段的氧化过程被观察:(1 ) 起始的氧化阶段被 Cu 2 经由稳固固体的转变的 O NC,导致一个新中间的 CuO x 阶段的形成。CuO x 阶段的可能的水晶结构被讨论。(2 ) 随后, CuO x 被 layer-by-layer 氧化转变成 CuO。这些结果将在理解铜氧化物的氧化机制帮助并且为改进他们的结构的差异并且利用他们的潜在的工业应用铺平道路。 | Huihui Liu He Zheng Lei Li Huaping Sheng Shuangfeng Jia Fan Cao Xi Liu Boyun Chen Ru Xing Dongshan Zhao Jianbo Wang | 2017 | Nano Research2017，10，7： | 4 |
| 6 | Association between Hypertension with Hyperhomocysteinemia and Cognitive Impairment in the Kailuan Community of China:A Cross-sectional Study显示文摘Cognitive impairment,especially dementia,is common in elderly individuals and seriously affects life quality.Dementia is the most severe expression of cognitive impairment characterized by a progressive functional decline in one or more cognitive domains.The prevalence of dementia has increased dramatically,and the number of patients with dementia will reach 130 million in 2050.The worldwide prevalence of dementia among elderly individuals is approximately 5.0%.In China,more than 220 million people aged over 60 years in 2016. | CHEN Xue Yu YANG Yao TANG Zhao Yang LYU Jian SHI Feng Xue CHEN Yan Ru WANG Xiao Hui TAN Tan XING Wei Jia HOU Hai Feng JI Long LI Xiao Dong LI Dong | 2021 | Biomedical and Environmental Sciences2021，34，7： | 3 |
| 7 | Optimal Waist Circumference Cut-off values for Identifying Metabolic Risk Factors in Middle-aged and Elderly Subjects in Shandong Province of China显示文摘Objective To study the optimal waist circumference(WC) cut-off values for identifying metabolic risk factors in middle-aged and elderly subjects in Shandong Province of China. Methods A total of 2 873 men and 5 559 women were included in this cross-sectional study. Metabolic syndrome(MetS) was diagnosed according to the definition of Chinese Diabetes Society in 2004. The relation between WC and MetS was analyzed by multivariate logistic regression analysis. The optimal WC cut-off values were identified using the area under the ROC curve and the different diagnostic criteria for central obesity were compared. Results The WC was the risk factor for MetS independent of BMI, blood glucose, blood lipid, and blood pressure. The optimal WC cut-off value was 83.8 cm and 91.1 cm for identifying MetS in women and men, respectively. Compared with 80 cm and 85 cm for women and men, 85 cm and 90 cm had a higher Youden index for identifying all metabolic risk factors and MetS in women and men. Conclusion The appropriate WC cut-off value is 85 cm and 90 cm for identifying central obesity and MetS in women and men in Shandong Province of China. | HOU Xin Guo WANG Chuan MA Ze Qiang YANG Wei Fang WANG Ji Xiang LI Cheng Qiao WANG Yu Lian LIU Shu Min Hu Xiu Ping ZHANG Xiu Ping JIANG Mei WANG Wei Qing NING Guang ZHENG Hui Zhen MA Ai Xia SUN Yu SONG Jun LIN Peng LIANG Kai LIU Fu Qiang LI Wen Juan XIAO Juan GONG Lei WANG Mei Jian LIU Ji Dong YAN Fei YANG Jun Peng WANG Ling Shu TIAN Meng ZHAO Ru Xing JIANG Ling CHEN Li | 2014 | Biomedical and Environmental Sciences2014，27，5： | 3 |
| 8 | Effect of long-term fertilization on soil nitrate distribution显示文摘A thirteen years long term field fertilizer experiment was conducted to monitor the effect of different fertilization on soil nitrate distribution. The results showed: (1) Applying relative excessive N fertilizer could result large quantities of NO - 3 residue and NO - 3 movement downward in soil profiles; amending phosphate fertilizer or organic manure with nitrogen fertilizer together could significantly improve the status of NO - 3 leaching downward due to the balanced uptake of nutrients by crops. (2) Appropriate amounts of nitrogen fertilizer which was equal or smaller than the optimal fertilization rate could not result in more NO - 3 leaching in Northern China. (3) Precipitation influenced the amounts and depth of soil NO\+-\-3 leaching: NO - 3 could move to 80 cm depth or below at autumn or at the next spring when rainfall was higher during the rainy season through July to September in North China. | GUO Li ping *, ZHANG Fu suo, WANG Xing ren, MAO Da ru, CHEN Xin ping (Department of Plant Nutrition, China Agricultural University, Beijing 100094, China. E mail: guolp@ns.ami.ac.cn) | 2001 | Journal of Environmental Sciences2001，13，1： | 3 |
| 9 | Synthesis and anti-TMV activity of novel N-(pyrimidin-5-yl)-N'-phenylureas显示文摘In order to find novel leading structures of pesticide,a series of N-(pyrimidin-5-yl)-N'-phenylureas(4a-4h) were designed and synthesized from 4-chloro-2-diethylamino-6-methyl-5-nitropyrimidine 1 via substitution,reduction and acylation procedures. Preliminary bioassay showed that all the target compounds processing good anti-TMV(tobacco mosaic virus) activity.Two compounds(4d and 4e) displayed higher activity superior to virazole at a concentration of 5.0×10^(-4)g/mL. | De Kai Yuan Da Qiang Zhang Ru Xing Li Dao Quan Wang Xin Ling Yang | 2011 | Chinese Chemical Letters2011，22，1： | 2 |
| 10 | Dynamic Response Analysis of Working Device of Hydraulic Excavator under Working Impact Loading显示文摘 | Guo Li Xin Xie Hua Long Zhou Shu Wen Li Jin Li Wang Zhao Wen Xing Ru Fei | 2009 | Applied Mechanics and Materials2009，，16： | 1 |
| 11 | Cloning a second form of adenine phosphoribosyl transferase gene(TaAPT2) from wheat and analysis of its association with thermo-sensitive genic male sterility(TGMS)显示文摘 | XING Q H Ru Z G Li J | 2005 | Plant Science2005，169，： | 1 |
| 12 | GeC film with high substitutional carbon concentration formed by ion implantation and solid phase epitaxy for strained Ge n-MOSFETs显示文摘Ge is considered as a promising channel materialto replace Si because of its high carrier mobil-ity than Si and compatibility with conventional Siprocess [1-3]. Strain engineering has been widelyused in Si-based devices and can also be adoptedin Ge-based devices tofurther improve devicereported that electronextend scaling limits andperformance. It has beenand hole mobility of Geare boosted by compressive and tensile strain. | Bingxin ZHANG Xia AN Xiangyang HU Ming LI Xing ZHANG Ru HUANG | 2018 | Science China(Information Sciences)2018，61，6： | 1 |
| 13 | Formation of β-SiC nanowires by annealing SiC films in hydrogen atmosphere显示文摘 | Li Yang Xing Zhang Ru Huang Guoyan Zhang Chengshan Xue | 2006 | Physica E: Low-dimensional Systems and Nanostructures2006，，1： | 1 |
| 14 | A Virtual Human’s Driving Method Based on Motion Capture Data显示文摘 | Li Hua Zhang Xue Ying Nai Dong Hai Zhu Cheng Cai Zhu Xing Ru Zhang Wu Li | 2013 | Advanced Materials Research2013，，711： | 1 |
| 15 | Dehalogenation of Aryl Halides Catalyzed by MontK10 Immobilized PVP-Pd-Sn Catalyst in Aqueous System显示文摘A series of PVP-Pd-Sn/MontK10 catalysts were prepared by immobilization of PVP[poly(N-vinyl-2-pyrrolidone)] supported bimetallic catalyst using MontK10 as carrier. This catalyst has good catalytic activity for hydrogen transfer dehalogenation of aryl halides. The catalytic reaction was carried out in aqueous system in the presence of phase transfer catalyst and sodium formate as hydrogen source. The catalyst with loading Pd 0.19wt% and molar ratio of Pd/Sn 8:1 gives the highest activity and good stability. This catalyst is more reducible with NaBH4. It is also found that the catalyst is easily separated from the reaction system. | Xiao Li ZHEN1, Jian Rong HAN2, Ru Hong KANG2*, Xing Mei OUYANG2 1Department of Essential Education, Hebei Science ( Technique University, Shijiazhuang 050018 2Department of Chemistry, Heibei Normal University, Shijiazhuang 050016 | 2001 | Chinese Chemical Letters2001，12，4： | 1 |
| 16 | Cloning a second form of adenine phosphoribosyl transferase gene (TaAPT2) from wheat and analysis of its association with thermo-sensitive genic male sterility (TGMS)显示文摘 | Xing Q H Ru Z G Li J | 2005 | Plant Sci2005，169，： | 1 |
| 17 | Two-step synthesis of one-dimensional single crystalline GaN nanowires显示文摘 | Yang Li Zhang Xing Huang Ru | 2005 | Physica E2005，25，： | 1 |
| 18 | Improvement of thermal stability of nickel germanide using nitrogen plasma pretreatment for germanium-based technology显示文摘Dear editor,Ge has been considered as a very promising candidate as a channel material of MOSFET to extend Moore’s law beyond sub-7 nm node, due to its high and symmetric mobility, as well as the compatibility with conventional Si process [1, 2].In spite of higher carrier mobility than Si, there are still several challenges for Ge MOSFETs, such as low electrical concentration of n-type dopants and high electron Schottky barrier height, resulting in large source/drain (S/D) series resistance. | Bingxin ZHANG Xia AN Pengqiang LIU Xiangyang HU Ming LI Xing ZHANG Ru HUANG | 2018 | Science China(Information Sciences)2018，61，10： | 1 |
| 19 | Ge surface passivation by GeO_2 fabricated by N_2O plasma oxidation显示文摘In this paper, Ge surface passivation by Ge O2 grown by N2 O plasma oxidation is presented and experimentally demonstrated. Results show that stoichiometrically Ge O2 can be achieved by N2 O plasma oxidation at 350°C. The transmission electron microscope observation reveals that the Ge O2/Ge interface is automatically smooth and the thickness of Ge O2is~0.9 nm with 120 s N2 O plasma oxidation. The interface state density of Ge surface after N2 O plasma passivation is about~3×1011 cm-2e V-1. With Ge O2 passivation,the hysteresis of MOS capacitor with Al2O3 as gate dielectric is reduced to~55 m V, compared to 130 m V of the untreated one. The Fermi-level at Ge O2/Ge interface is unpinned, and the surface potential is effectively modulated by the gate voltage, which is promising for high performance NMOSFETs fabrication. | LIN Meng AN Xia LI Ming YUN QuanXin LI Min LI Zhi Qiang LIU PengQiang ZHANG Xing HUANG Ru | 2015 | Science China(Information Sciences)2015，58，4： | 1 |
| 20 | Cloning a second form of adenine phosphoribosyl transferase gene ( TaAPT2) from wheat and analysis of its association with thermo-sensitive genic male sterility (TGMS)显示文摘 | Quanhua Xing Zhengang Ru Jun Li Chunjiang Zhou Demin Jin Yan Sun Bin Wang | 2005 | Plant Science2005，，1： | 1 |
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