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| 1 | Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues显示文摘BACKGROUND Mesenchymal stromal/stem cells (MSCs) constitute a promising tool in regenerative medicine and can be isolated from different human tissues. However, their biological properties are still not fully characterized. Whereas MSCs from different tissue exhibit many common characteristics, their biological activity and some markers are different and depend on their tissue of origin. Understanding the factors that underlie MSC biology should constitute important points for consideration for researchers interested in clinical MSC application. AIM To characterize the biological activity of MSCs during longterm culture isolated from: bone marrow (BM-MSCs), adipose tissue (AT-MSCs), skeletal muscles (SMMSCs), and skin (SK-MSCs). METHODS MSCs were isolated from the tissues, cultured for 10 passages, and assessed for: phenotype with immunofluorescence and flow cytometry, multipotency with differentiation capacity for osteo-, chondro-, and adipogenesis, stemness markers with qPCR for mRNA for Sox2 and Oct4, and genetic stability for p53 and c-Myc;27 bioactive factors were screened using the multiplex ELISA array, and spontaneous fusion involving a co-culture of SM-MSCs with BM-MSCs or AT-MSCs stained with PKH26 (red) or PKH67 (green) was performed. RESULTS All MSCs showed the basic MSC phenotype;however, their expression decreased during the follow-up period, as confirmed by fluorescence intensity. The examined MSCs express CD146 marker associated with proangiogenic properties;however their expression decreased in AT-MSCs and SM-MSCs, but was maintained in BM-MSCs. In contrast, in SK-MSCs CD146 expression increased in late passages. All MSCs, except BM-MSCs, expressed PW1, a marker associated with differentiation capacity and apoptosis. BM-MSCs and AT-MSCs expressed stemness markers Sox2 and Oct4 in long-term culture. All MSCs showed a stable p53 and c-Myc expression. BM-MSCs and AT-MSCs maintained their differentiation capacity during the follow-up period. In contrast, SK-MSCs and SM-MSCs had a limited ability to differentiate into adipocytes. BM-MSCs and AT-MSCs revealed similarities in phenotype maintenance, capacity for multilineage differentiation, and secretion of bioactive factors. Because AT-MSCs fused with SM-MSCs as effectively as BM-MSCs, AT-MSCs may constitute an alternative source for BM-MSCs. CONCLUSION Long-term culture affects the biological activity of MSCs obtained from various tissues. The source of MSCs and number of passages are important considerations in regenerative medicine. | Urszula Kozlowska Agnieszka Krawczenko Katarzyna Futoma Tomasz Jurek Marta Rorat Dariusz Patrzalek Aleksandra Klimczak | 2019 | World Journal of Stem Cells2019，11，6： | 10 |
| 2 | Isolation and expression of cold specific genes in potato (Solanum sogarandinum)显示文摘 | Rorat T Wojciech J G Pierre B | 1998 | Plant Science1998，133，1： | 1 |
| 3 | Liver steatosis in Polish children assessed by medicolegal autopsies 显示文摘 | Rorat M Jurek T Kuchar E | 2013 | World J Pediatr2013，9，1： | 1 |
| 4 | Plant dehydrins-tissue location, structure and function 显示文摘 | RORAT T | 2006 | Cell Mol Biol Lett2006，11，4： | 1 |
| 5 | The organ-dependent abundance of a solanum lipid transfer protein is up-regulated upon osmotic constraints and associated with cold acclimation ability显示文摘 | Kielbowicz-Matuk A Rey P Rorat T | 2008 | Journal of Experimental Botany2008，59，8： | 1 |
| 6 | Expression of SK3-type dehydrin in transporting organs is associated with cold acclimation in Solanum species显示文摘 | Rorat T Szabala B M Grygorowicz W J Wojtowicz B Yin Z Rey P | | 0，，01： | 1 |
| 7 | Expression of a Solanum sogarandinum SK 3 -type dehydrin enhances cold tolerance in transgenic cucumber seedlings显示文摘 | Zhimin Yin Tadeusz Rorat Bartosz M. Szabala Agnieszka Zió?kowska Stefan Malepszy | 2006 | Plant Science2006，，6： | 1 |
| 8 | Plant dehydrins-tissue location, structure and function 显示文摘 | Rorat T | 2006 | Cellular & Moleeular Biology Letters2006，11，4： | 1 |
| 9 | The clinical course of late diagnosed fatal cases of A ( H1N1 ) influ- enza in Poland 显示文摘 | RORAT M JUREK T KUCHAR E | 2013 | Postepy Hig Med Dosw ( Online)2013，67，： | 1 |
| 10 | Plant dehydrins--tissue location, structure and function显示文摘 | Rorat T | 2006 | Cell Mol Biol Lett2006，11，4： | 1 |
| 11 | Plant dehydrins-tissue location, structure and function显示文摘 | Rorat T | 2006 | Cell Mol Biol Lett2006，11，： | 1 |
| 12 | Expression of SK3-type dehydrin in transporting organs is associated with cold acclimation in Solanum species显示文摘 | RORAT T SZABALA B M GRYGOROWICZ W J etal | 2006 | Planta2006，224，： | 1 |
| 13 | Expression of a Solarium sogarandinurn SK3-type dehydrin enhances cold tolerance in transgenic cucumber seedlings显示文摘 | YIN Z M RORAT T SZABALA B M etal | 2006 | Plant Science2006，170，： | 1 |
| 14 | Expression of SK3-type dehydrin in transporting organs is associated with cold acclimation in Solanum species显示文摘 | RORAT T SZABALA B M GRYGOROWICZ W J WOJTOWICZ B YIN Z REY P | 2006 | Planm2006，224，1： | 1 |
| 15 | Expression of a Solanum sogarandinum SK3-type dehydrin en-hances cold tolerance in transgenic cucumber seedlings显示文摘 | YIN Z RORAT T SZABALA B M ZIOLKOWSKA A MALEPSZY S | 2006 | PlantSci2006，170，6： | 1 |
| 16 | Plant dehydrins-tissue location, structure and function 显示文摘 | RORAT T | 2006 | Cellular & Molecular Biology Letters2006，11，： | 1 |
| 17 | Expression of KS-type dehydrins is primarily regulated by factors related to organ type and leaf developmental stage during vegetative growth显示文摘 | T. Rorat W. J. Grygorowicz W. Irzykowski P. Rey | 2004 | Planta2004，，5： | 1 |
| 18 | Mixed tumors of the vulva: clinical outcome and pathology显示文摘 | Rorat E Wallach RC | 1984 | Int J Gynecol Pathol1984，3，3： | 1 |
| 19 | Liver steatosis in Polish children assessed by medicolegal autopsies显示文摘 | Marta Rorat Tomasz Jurek Ernest Kuchar Leszek Szenborn Wojciech Golema Agnieszka Halon | 2013 | World Journal of Pediatrics2013，，1： | 1 |
| 20 | Plant dehydrins — Tissue location, structure and function显示文摘 | Tadeusz Rorat | 2006 | Cellular & Molecular Biology Letters2006，，4： | 1 |
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