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| 1 | Epigenetic modification regulates both expression of tumor-associated genes and cell cycle progressing in human colon cancer cell lines: Colo-320 and SW1116显示文摘The aim of this study is to assess the effects of DNA methylation and histone acetylation, alone or in combination, on the expression of several tumor-associated genes and cell cycle progression in two established human colon cancer cell lines: Colo-320 and SW1116. Treatments with 5-aza-2'-deoxycytidine (5-aza-dC) and trichostatin A, alone or in combination, were applied respectively. The methylation status of the CDKN2A promoter was determined by methylation-specific PCR, and the acetylated status of the histones associated with the p21wAF1 and CDKN2A genes was examined by chromatin immunoprecipitation. The expression of the CDKN2A, p21WAF1, p53, p73, APC, c-myc, c-Ki-ras and survivin genes was detected by real-time RT-PCR and RT-PCR. The cell cycle profile was established by flow cytometry.We found that along with the demethylation of the CDKN2A gene promoter in both cell lines induced by 5-aza-dC alone or in combination with TSA, the expression of both CDKN2A and APC genes increased. The treatment of TSA or sodium butyrate up-regulated the transcription of p21 WAF1 significantly by inducing the acetylation of histones H4 and H3, but failed to alter the acetylation level of CDKN2A-associated histones. No changes in transcription of p53, p73,c-myc, c-Ki-ras and survivin genes were observed. In addition, TSA or sodium butyrate was shown to arrest cells at the G1 phase. However, 5-aza-dC was not able to affect the cell cycle progression. In conclusion, regulation by epigenetic modification of the transcription of tumor-associated genes and the cell cycle progression in both human colon cancer cell lines Colo-320 and SW1116 is gene-specific. | JingYuanFANG YingXuanCHEN JuanLU RongLU LiYANG HongYinZHU WeiQiGU LunGenLU | 2004 | Cell Research2004，14，3： | 46 |
| 2 | Expression of Dnmt1,demethylase,MeCP2 and methylation of tumor-related genes in human gastric cancer显示文摘AIM: To explore the effect of DNA methyltransferase,demethylase and methyl-CpG binding protein MeCP2 on the expressions and methylation of hMSH2 and protooncogene in human gastric cancer.METHODS: Paired samples of primary gastric cancer and corresponking para-cancerous, non-cancerous gastric mucosae were obtained from surgically resected specimens of 28 patients. TranscriplJon levels of Dnmtl, mbd2, MeCP2, p16^INK4A,hMSH2 and c-myc were detected by using real-time PCR or RT-PCR. Promoter methylation of p16^INK4A, c-myc and hMSH2 genes was assayed by methylation-specific PCR (MSP) and sequencing (mapping). Their relationships were analyzed by Fisher's exact test using the software SPSS.RESULTS: The average mRNA level of Dnmtl gene from cancerous tissue was higher and that of mbd2 gene from cancerous tissue was lower than that from non-cancerous tissue, respectively, mbd2 was lower in cancerous tissue than in non-cancerous tissue in 14 (50.0%) of patients but higher in 3 cases (10.7%) of non-cancerous gastric tissue(P<0.001). c-myc expression was up-regulated in cancer tissues (P<0.05). The up-regulation of mbd2 was found in all patients with hypomethyiated c-myc. The transcriptional levels of p16^IK4A and MeCP2 genes did not display any differenoe between gastric cancerous and matched non-cancerous tissues. There were down-regulation and hypermethylation of hMSH2 in cancer tissues, and the hypermethylation of hMSH2 coexisted with down-regulated transcription.However, the transcription level of the above genes was not associated with biological behaviours of gastric cancers.CONCLUSION: The up-regulation of proto-oncogene may be the consequence of epigenetic control of gene expression by demethylase, and mbd2 is involved in the regulation of hMSH2 expression in human gastric cancer. | Jing-YuanFang Zhong-HuaCheng Ying-XuanChen RongLu LiYang Hong-YinZhu Lun-GenLu | 2004 | World Journal of Gastroenterology2004，10，23： | 19 |
| 3 | Histone acetylation regulates p21^(WAF1) expression in human colon cancer cell lines显示文摘AIM: To investigate the effect of histone acetylation on regulation of p21^WAF1 gene expression in human colon cancer cell lines.METHODS: Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate.Expressions of p21^WAF1 mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively.Acetylation of two regions of p21^WAF1 gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay and Western blotting.RESULTS: Trichostatin or sodium butyrate re-activated p21^WAF1 transcription resulted in up-regulated p21^WAF1 protein level in colon cancer cell lines. Those effects were accompanied by an accumulation of acetylated histones in total cellular chromatin and p21^WAF1 gene-associated region of chromatin.CONCLUSION: Histone acetylation regulates p21^WAF1 expression in human colon cancer cell lines, Colo-320 and SW1116. | Ying-XuanChen Jing-YuanFang Hong-YinZhu RongLu Zhong-HuaCheng De-KaiQiu | 2004 | World Journal of Gastroenterology2004，10，18： | 4 |
| 4 | Improvements of Thorp- Rolewicz Theorems on Operator Series 显示文摘 | LI Ronglu CUI Chengri CHO Minhyung | 1998 | Bull Korean Math1998，35，1： | 1 |
| 5 | A new uniform boundedness principle显示文摘 | Zhong Shuhui Cui Chengri | | to appear0，，： | 1 |
| 6 | Spaces for which the uniform boundedness principle holds显示文摘 | Swartz C | 1992 | Studia Sci Math Hungar1992，，27： | 1 |
| 7 | The Strongest Intrinsic Meaning of Sequential-evaluation Convergence 显示文摘 | LI Ronglu WANG Fubin ZHONG Shuhui | 2007 | Topology and Its Applications2007，154，： | 1 |
| 8 | Impulsive Functional Differential Inclusions and Fuzzy Population Models显示文摘 | GUO Mengshu XUE Xiaoping LI Ronglu | 2003 | Fuzzy Sets and Systems2003，138，3： | 1 |
| 9 | A new open mapping theorem显示文摘 | Zhong Shuhui | | to appear0，，： | 1 |
| 10 | A new closed graph theorem显示文摘 | Zhong Shuhui | | to appear0，，： | 1 |
| 11 | Microstructure and hardness of laser clad SiCP-Al composite coatings on Al alloys 显示文摘 | Sun Ronglu Lei Yiwen | 2008 | Materials Letters2008，62，1718： | 1 |
| 12 | Numerical simulation of temperature distribution and TiC growth kinetics for high power laser clad TiC/NiCrBSiC composite coatings 显示文摘 | Lei Yiwen Sun Ronglu Tang Ying | 2012 | Optics & Laser Technology2012，，44： | 1 |
| 13 | Microstructure and hardness of laser clad SiCp-Al composite coatings on Al alloys显示文摘 | Sun Ronglu Lei Yiwen | 2008 | Mater Lett2008，62，： | 1 |
| 14 | Microstructure and hardness of laser clad SiCp-Al composite coatings on AI alloys 显示文摘 | Sun Ronglu Lei Yiwen | 2008 | Materials Letters2008，62，1718： | 1 |
| 15 | New Basic Principles of Functional Analysis显示文摘 | Zhong Shuhui Cui Chengri | 2004 | Journal of Yanbian University (Natural Science)2004，30，3： | 1 |
| 16 | Ex- perimental and thermodynamic investigations into the microstructure of laser clad A1-Si coatings on AZglD al- loys显示文摘 | LEI YIWEN SUN RONGLU TANG YING | 2012 | Surface and Coatings Technology2012，，207： | 1 |
| 17 | On the gliding hump property显示文摘 | LI Ronglu CHO Min-Hyung | 1999 | Taiwan Journal of Math1999，3，： | 1 |
| 18 | Continuity and boundedness for operator-valued matrix mappings显示文摘 | WU Junde LI Ronglu Swartz C | 1998 | Taiwan Journal of Math1998，4，： | 1 |
| 19 | Numericalsimulation of temperature distribution and TiC growth kineticsfor high power laser clad TiC/NiCrBSiC composite coatings显示文摘 | Lei Yiwen Sun Ronglu | 2012 | Optics & Laser Technology2012，，44： | 1 |
| 20 | Trichostatin A, a histone deacetylase inhibitor, suppresses JAK2/STAT3 signaling via inducing the promoter‐associated histone acetylation of SOCS1 and SOCS3 in human colorectal cancer cells显示文摘 | HuaXiong WanDu Yan‐JieZhang JieHong Wen‐YuSu Jie‐TingTang Ying‐ChaoWang RongLu Jing‐YuanFang | 2012 | Mol Carcinog2012，，2： | 1 |
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