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您的检索式：作者名="Rongfeng Qu"
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| 1 | N^(6)-methyladenosine methyltransferase plays a role in hypoxic preconditioning partially through the interaction with lncRNA H19显示文摘N^(6)-methyladenosine(m6A),a m ethylation in the N6 position of adenosine especially in the mRNA,exerts diverse physiological and pathological functions.However,the precise role of m 6A methylation in hypoxic preconditioning(HPC)is still unknown.Here,we observed that HPC treatm ent protected H9c2 cells against H2O2-induced injury,upregulated the m 6A level in the total RNA and the expression of methyltransferase like 3(METTL3),methyltransferase like 14(METTL14),and long noncoding RNA(lncRNA)H19.Either knockdown of METTL3 or METTL14 notably reversed the HPC-induced enhancement of cell viability,anti-apoptosis ability,and H19 expression.M ethylated RNA im m unoprecipitation(IP)indicated that knockdown of METTL3 or METTL14 decreased m 6A level in the lncRNA H19.Gain-of-function assay dem onstrated that H19 overexpression could partially rescue the decreased protection mediated by METTL3 or METTL14 knockdown in HPCtreated H9c2 cells.RNA binding protein im m unoprecipitation(RIP)assay showed that METTL3 and METTL14 could directly bind w ith H19.Our study identified a novel pattern of posttranscriptional regulation in HPC treatm ent.Since METTL3,METTL14,and lncRNA H19 were involved in HPC protection,they could be considered as potential biomarkers and therapeutic targets in HPC-derived cardiac rehabilitation and therapeutic approaches. | Yamin Su Rongfeng X u Rui Zhang Yangyang Qu Wenjie Zuo Zhenjun Ji Haihua Geng Min Pan Genshan Ma | 2020 | Acta Biochimica et Biophysica Sinica2020，52，12： | 1 |
| 2 | An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo显示文摘Background: Male germline stem cells(MGSCs) are a subpopulation of germ cells in the testis tissue. MGSCs are capable of differentiation into spermatozoa and thus are perfect targets for genomic manipulation to generate transgenic animals.Method: The present study was to optimize a protocol of production of transgenic mice through transduction of MGSCs in vivo using lentiviral-based vectors. The recombinant lentiviral vectors with either EF-1 or CMV promoter to drive the expression of enhanced green fluorescent protein(e GFP) transgene were injected into seminiferous tubules or inter-tubular space of 7-day-old and 28-day-old mouse testes. At 5 or 6 wk post-surgery, these pre-founders were mated with wild-type C57BL/6J female mice(1.5 to 2.0-month-old).Results: Sixty-seven percent of F1 generation and 55.56 % of F2 offspring were positive for eG FP transgene under the control of EF-1 promoter via PCR analysis. The transgenic pups were generated in an injection site-and age-independent manner. The expression of transgene was displayed in the progeny derived from lentiviral vector containing CMV promoter to drive transgene, but it was silenced or undetectable in the offspring derived from lentiviral vector with transgene under EF-1 promoter. The methylation level of g DNA in the promoter region of transgene was much higher in the samples derived lentiviral vectors with EF-1 promoter than that with CMV promoter,suggesting e GFP transgene was suppressed by DNA methylation in vivo.Conclusion: This research reported here an effective strategy for generation of transgenic mice through transduction of MGSCs in vivo using lentivirus vectors with specific promoters, and the transgenic offspring were obtained in an injection site-and age-independent manner. This protocol could be applied to other animal species, leading to advancement of animal transgenesis in agricultural and biomedical fields. | Jinzhou Qin Haixia Xu Pengfei Zhang Conghui Zhang Zhendong Zhu Rongfeng Qu Yuwei Qin Wenxian Zeng | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 1 |
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