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| 1 | Biofilm formation in clinical isolates of nosocomial Acinetobacter baumannii and its relationship with multidrug resistance显示文摘Objective: To check biofilm formation by Acinetobacter baumannii(A. baumannii)clinical isolates and show their susceptibility to different antibiotics and investigate a possible link between establishment of biofilm and multidrug resistance.Methods: This study was performed on clinical samples collected from patients with nosocomial infections in three hospitals of Tehran. Samples were initially screened by culture and biochemical tests for the presence of different species of Acinetobacter. Identifications were further confirmed by PCR assays. Their susceptibilities to 11 antibiotics of different classes were determined by disc diffusion method according to Clinical and Laboratory Standards Institute guidelines. The ability to produce biofilm was investigated using methods: culture on Congo red agar, microtiter plate, and test tube method.Results: From the overall clinical samples, 156 specimens were confirmed to contain A. baumannii. The bacteria were highly resistant to most antibiotics except polymyxin B.Of these isolates, 10.26% were able to produce biofilms as shown on Congo red agar.However, the percentage of bacteria with positive biofilm in test tube, standard microtiter plate, and modified microtiter plate assays were 48.72%, 66.66%, and 73.72%, respectively. At least 92% of the biofilm forming isolates were multidrug resistant.Conclusions: Since most of the multidrug resistant strains produce biofilm, it seems necessary to provide continuous monitoring and determination of antibiotic susceptibility of clinical A. baumannii. This would help to select the most appropriate antibiotic for treatment. | Ebrahim Babapour Azam Haddadi Reza Mirnejad Seyed-Abdolhamid Angaji Nour Amirmozafari | 2016 | Asian Pacific Journal of Tropical Biomedicine2016，6，6： | 13 |
| 2 | The aflatoxin B1 isolating potential of two lactic acid bacteria显示文摘Objective:To determine lactic acid bacteria's capability to enhance the process of binding and isolating aflatoxin B1 and to utilize such lactic acid bacteria as a food supplement or probiotic products for preventing absorption of aflatoxin Bl in human and animal bodies.Methods:In the present research,the bacteria were isolated from five different sources.For surveying the capability of the bacteria in isolating aflatoxin Bl,ELISA method was implemented,and for identifying the resultant strains through 16S rRNA sequencing method,universal primers were applied.Results:Among the strains which were isolated,two strains of Lactobacillus pentosus and Lactobacillus beveris exhibited the capability of absorbing and isolating aflatoxin Bl by respectively absorbing and discharging 17.4%and 34.7%of the aforementioned toxin existing in the experiment solution.Conclusions:Strains of Lactobacillus pentosus and Lactobacillus beveris were isolated from human feces and local milk samples,respectively.And both strains has the ability to isolate or bind with aflatoxin Bl. | Adel Hamidi Reza Mirnejad Emad Yahaghi Vahid Behnod Ali Mirhosseini Sajad Amani Sara Sattari Ebrahim Khodaverdi Darian | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，9： | 8 |
| 3 | A duplex PCR for the rapid and simultaneous detection of Brucella spp.in human blood samples显示文摘Objective:To design a duplex PCR for rapid and simultaneous detection of Brucella species, in human blood samples.Methods:Fifty-two peripheral bloods samples were collected from suspicious patients with brucellosis.Following DNA extraction,PCR assay were performed, using three primers that could simultaneously identify and differentiate three major species of pathogenic Brucella in humans and animals.Results:Of the 52 peripheral bloods samples tested, 25 sample(48%) showed positive reactions in PCR.Twelve samples were positive for Brucella abortus(B.abortus)(23%,13 for Brucella melUensis(B.melUensis)(25%) and 0 for Brucella ovis (6.ovis)(Ow.Conclusions:This work de=monstrates dial in case where specific primers were utilized,duplex PCR has proved to be a simple,fast,and relatively inexpensive method for simultaneous detection of important species of Brucella in clinical samples. | Reza Mirnejad Mozafar mohamadi Vahbeh Piranfar Seied Mojtaba Mortazavi Reza Kachuei | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，6： | 6 |
| 4 | Simultaneous detection and differentiates of Brucella abortus and Brucella melitensis by combinatorial PCR显示文摘Objective:To evaluate simultaneous detection and differentiates of Brucella abortus(B.abortus) and Brucella melitensis(B.melitensis) through the combinatorial POR method.Methods:This study was designed using three primers that could simultaneously identify and differentiate two major species of pathogenic Brucella in humans and animals.Identification and differentiation of each species using the size of the PCR product were determined.To determine the specifieity of the method,bacteria close to the genus Brucella were used.Finally,to confirm PCR products.In addition to the products sequence,RFLP was performed on PCR products using restriction enzymes.Results:The method of optimized combinatorial PCR in this study could simultaneously detect and differentiate B.abortus and B.melitensis with high specificity and sensitivity in clinical samples.Differentiation of species is based on the resulting bands: therefore,the band 494 bp for B.abortus and 733 bp for B.melitensis were obtained.RFLP and sequencing results confirmed PCR results.Conclusions:The results of this study shows that without routine diagnostic methods such as culture and serology tests,using the molecular method of combinatorial PCR,important species of Brucella can be simultaneously identified and differentiated in clinical samples. | Reza Mirnejad Reza Hosseini Doust Reza Kachuei Seied Mojtaba Mortazavi Mehdi Khoobdel Ali Ahamadi | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，1： | 5 |
| 5 | Antibiotic resistance and carriage class 1 and 2 integrons in clinical isolates of Acinetobacter baumannii from Tehran,Iran显示文摘Objective:To investigate antibiotic resistance and carriage class 1 and 2 integrons in clinical isolates of Acinetobacter baumannii(A.baumannii)from Tehran,Iran.Methods:Antimicrobial susceptibility testing was performed according to the Clinical and Laboratory Standards Institute.The presence of integrons was investigated by PCR using specific primers.Results:Among isolated A.baumannii strains,82%were multidrug resistant,27 samples(54%)were resistant to three or more than three antibiotics and 16 samples(32%)showed resistance to two antibiotics.Integrons were detected from 44 of 50 isolates(88%),with classes 1 and 2 being observed in 42%(21/50)and 82%(41/50)of isolates,respectively.Integron-positive A.baumannii isolates showed higher antibiotic resistance than integron-negative isolates and all showed a multidrug-resistant phenotype.Conclusions:Our findings show that classes 1 and 2 integrons,and especially classes 2 integrons are widely disseminated among A.baumannii strains isolated from Tehran and these structures are playing a major role in the acquisition of multidrug resistance in these strains.So monitoring of drug resistance with investigating carriage class 1 and 2 integrons is very important to plan specific infection control measures due to multidrug resistance A.baumannii in Iran hospitals. | Reza Mirnejad Sepideh Mostofi Faramaz Masjedian | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，2： | 5 |
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