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| 1 | GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm显示文摘Background: Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination(AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments,which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm(i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine reproduction.Results: Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar sperm.However, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor(PFE) than in good(GFE) freezability ejaculates in both pre-frozen(1.00 INT·mm^2± 0.14 INT·mm^2 vs.0.72 INT·mm^2± 0.15 INT·mm^2;P < 0.05) and post-thawed(0.96 INT·mm^2± 0.20 INT·mm^2 vs. 70 INT·mm^2± 0.19 INT·mm^2;P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species(ROS) production, and high membrane lipid disorder postthaw(P < 0.05).Conclusions: The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar sperm. | Marc Llavanera Ariadna Delgado-Bermúdez Beatriz Fernandez-Fuertes Sandra Recuero Yentel Mateo Sergi Bonet Isabel Barranco Marc Yeste | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 4 |
| 2 | A polymorphism in the regulatory region of APOE associated with risk for Alzheimer's demen- tia显示文摘 | Bullido M J Artiga M J Recuero M | 1998 | Nat Genet1998，18，1： | 1 |
| 3 | Factors contributing to the uncertainty in circumaural earphone calibration for audiometric test 显示文摘 | RUIZ M FEUEREISEN B MACHON D RECUERO M | 2005 | Applied Acoustics2005，66，9： | 1 |
| 4 | Herpes simplex virus type I induces an incomplete autophagic response in human neuroblastoma cells显示文摘 | Santana S Bullido MJ Recuero M | 2012 | J Alzheimers Dis2012，30，： | 1 |
| 5 | Assessment of Noise Exposure During Commuting in the Madrid Subway显示文摘 | M. Tabacchi I. Pavón M. Ausejo C. Asensio M. Recuero | 2011 | Journal of Occupational and Environmental Hygiene2011，，9： | 1 |
| 6 | Herpes simplex virus type I induces an incomplete autophagic response in human neuroblastoma cells显示文摘 | Santana S Bullido MJ Recuero M | 2012 | J Alzheimers Dis2012，30，4： | 1 |
| 7 | Use of cone beam computed tomography in the diagnosis, planning and follow up of a typem dens invaginatus case 显示文摘 | Vier-Pelisser FV Pelisser A Recuero LC | 2012 | Int Endod J2012，45，2： | 1 |
| 8 | Community Noise Survey of the City of Valdivia,Chile显示文摘 | Sommerhoff J Recuero M Suárez E | 2004 | Applied Acoustics2004，65，7： | 1 |
| 9 | Muhilocus species tree analyses resolve the radiation of the widespread Bufo bufo species group ( Anura, Bufonidae) 显示文摘 | Recuero E Canestrelli D V6rtis J | 2012 | Mol Phylogenet Evol2012，62，： | 1 |
| 10 | Critical illness myopa thyandneuropathy显示文摘 | Latronieo N Fenzi F Recuero D | 1996 | Lancet1996，347，9015： | 1 |
| 11 | Herpes simplex vi-rus type I induces the accumulation of intracellular beta-amy-loid in autophagic compartments and the inhibition of the non-amyloidogenic pathway in human neuroblastoma cells 显示文摘 | Santana S Recuero M Bullido MJ | 2012 | Neurobiol Aging2012，33，2： | 1 |
| 12 | Incontinentia pigmenti 显示文摘 | Poziomezyk CS Recuero JK Bringhenti L | 2014 | An Bras Dermatol2014，89，1： | 1 |
| 13 | Mitochondrial phylogeography of the Iberian endemic frog Rana iberica,with implications for its conservation显示文摘Genetic characterization of species using phylogeographic approaches represents a basic reference to understand their evolutionary history as well as to identify conservation priorities to protect areas of particular interest regarding evolutionary potential.Even in well-studied regions such information is lacking for the majority of species,including many endemic species with reduced distribution ranges.We investigate the phylogeographic pattern of the Iberian frog Rana iberica,an endemic amphibian restricted to Central and North-Western Iberian Peninsula.Using mitochondrial sequences,we reconstruct the phylogeographic history of the species to test the effect of Quaternary climate changes on the evolutionary diversification of lineages,that is,the differentiation of mitochondrial lineages and the formation of genetic diversity melting pots,and integrate phylogeographic evidence for future conservation planning.Our results indicate the existence of 3 main mitochondrial lineages differentiated during the Upper Pleistocene.Both historical demographic analyses and climatic niche modeling show a strong effect of glacial climate changes,suggesting recurrent range contractions and expansions.Under such circumstances,differentiation took place most likely by isolation in allopatric interglacial refugia.Secondary lineage admixture in northern Portugal generated a broad mixed zone with highest nucleotide diversity.Given its particular evolutionary potential,its reduced distribution and eventual threats under current climate change scenario,conservation priorities should focus on the isolated lineage from Sierra de Guadalupe. | Jose TEIXEIRA Helena GONCALVES Nuno FERRAND Mario GARCIA-PARiS Ernesto RECUERO | 2018 | Current Zoology2018，64，6： | 1 |
| 14 | Use of cone beam computed tomography in the diagnosis,planning and follow up of a typeⅢdens invaginatus case显示文摘 | Vier-Pelisser FV Pelisser A Recuero LC | 2012 | Int Endod J2012，45，2： | 1 |
| 15 | Calculation of chloride dffusivity in cncrete from mgration eperiments, in non-steady-state conditions 显示文摘 | ANDRADE C CERVIGON D RECUERO A | 1994 | Cem Concr Res1994，24，7： | 1 |
| 16 | Sperm chromatin condensation as an in vivo fertility biomarker in bulls:a flow cytometry approach显示文摘Background:Genetic selection in cattle has been directed to increase milk production.This,coupled to the fact that the vast majority of bovine artificial inseminations(AI)are performed using cryopreserved sperm,have led to a reduction of fertility rates over the years.Thus,seeking sensitive and specific sperm biomarkers able to predict fertility rates is of vital importance to improve cattle reproductive efficiency.In humans,sperm chromatin condensation evaluated through chromomycin A3(CMA3)has recently been purported to be a powerful biomarker for sperm functional status and male infertility.The objectives of the present study were:a)to set up a flow cytometry method for simultaneously evaluating chromatin condensation and sperm viability,and b)to test whether this parameter could be used as a predictor of in vivo fertility in bulls.The study included pools of three independent cryopreserved ejaculates per bull from 25 Holstein males.Reproductive outcomes of each sire were determined by non-return rates,which were used to classify bulls into two groups(highly fertile and subfertile).Results:Chromatin condensation status of bovine sperm was evaluated through the combination of CMA3 and Yo-Pro-1 staining and flow cytometry.Sperm quality parameters(morphology,viability,total and progressive motility)were also assessed.Pearson correlation coefficients and ROC curves were calculated to assess their capacity to predict in vivo fertility.Sperm morphology,viability and total motility presented an area under the ROC curve(AUC)of 0.54,0.64 and 0.68,respectively(P>0.05),and thus were not able to discriminate between fertile and subfertile individuals.Alternatively,while the percentage of progressively motile sperm showed a significant predictive value,with an AUC of 0.73(P=0.05),CMA3/Yo-Pro-1 staining even depicted superior results for the prediction of in vivo fertility in bulls.Specifically,the percentage of viable sperm with poor chromatin condensation showed better accuracy and precision to predict in vivo fertility,with an AUC of 0.78(P=0.02).Conclusions:Chromatin condensation evaluated through CMA3/Yo-Pro-1 and flow cytometry is defined here as a more powerful tool than conventional sperm parameters to predict bull in vivo fertility,with a potential ability to maximising the efficiency of dairy breeding industry. | Marc Llavanera Jordi Ribas-Maynou Ariadna Delgado-Bermúdez Sandra Recuero Rodrigo Muiño Carlos OHidalgo Carolina Tamargo Sergi Bonet Yentel Mateo-Otero Marc Yeste | 2022 | Journal of Animal Science and Biotechnology2022，13，2： | 0 |
| 17 | Limited long-distance dispersal success in a Western European fairy shrimp evidenced by nuclear and mitochondrial lineage structuring显示文摘Anostraca are known by their ability for long-distance dispersal,but the existence in several species of deep,geographically structured mtDNA lineages suggests their populations are subjected to allopatric differentiation,isolation,and prevalence of local scale dispersion.Tanymastix stagnalis is one of the most widespread species of Anostraca and previous studies revealed an unclear geographical pattern of mtDNA genetic diversity.Here,we analyze populations from the Iberian and Italian Peninsulas,Central Europe,and Scandinavia,with the aim to characterize the patterns of genetic diversity in a spatio-temporal framework using mtDNA and nuclear markers to test gene flow among close populations.For these aims we built a time-calibrated phylogeny and carried out Bayesian phylogeographic analyses using a continuous diffusion model.Our results indicated that T.stagnalis presents a deeply structured genetic diversity,including 7 ancient lineages,some of them even predating the Pleistocene.The Iberian Peninsula harbors high diversity of lineages,with strong isolation and recent absence of gene flow between populations.Dispersal at local scale seems to be the prevailing dispersal mode of T.stagnalis,which exhibits a pattern of isolation-bydistance in the Iberian Peninsula.We remark the vulnerability of most of these lineages,given the limited known geographic distribution of some of them,and the high risk of losing important evolutionary potential for the species. | Paula C.RODRIGUEZ-FLORES Ernesto RECUERO Yolanda JIMENEZ-RUIZ Mario GARCIA-PARIS | 2020 | Current Zoology2020，66，3： | 0 |
| 18 | 非典型表现的神经纤维瘤病显示文摘Neurofibromatosis (NF) is considered to be a heterogeneous neuroectodermal disease clinically denned by the presence of neurofibromas, multiple caf′ e-au-lait spots, intertriginous freckles and Lisch nodules. Mosaicism explains atypical presentations of the disease. Early mutations, before tissue differentiation, give rise to generalized disease. We report an atypical presentation of neurofibromatosis with an unusual distribution of neurofibromas, a peculiar, clinically and pathologically, neurofibroma on the trunk and the association with an ovarian serous cystoadenofibroma. | Tato B.P. Sáez A.C. Recuero J.L.D. 吴佳纹 | 2006 | 世界核心医学期刊文摘(皮肤病学分册)2006，2，3： | 0 |
| 19 | Simulation, Construction and Characterization of a Piezoelectric Transducer Using Rexolite as Acoustic Matching显示文摘 | Israel Sanchez Dominguez Pedro Acevedo Contla Manuel Recuero Lopez Demetrio Fabian Garcia Nocetti | 2013 | 材料科学与工程(中英文B版)2013，3，4： | 0 |
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