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2篇 您的检索式:作者名="Rantao Zuo"
    题名 作者 年代 出处 被引量
1Higher Dietary Arachidonic Acid Levels Improved the Growth Performance, Gonad Development, Nutritional Value, and Antioxidant Enzyme Activities of Adult Sea Urchin(Strongylocentrotus intermedius)显示文摘The gonads of sea urchins(Strongylocentrotus intermedius) are characterized by high levels of arachidonic acid(ARA, 20:4 n-6) and eicosapentaenoic acid(EPA, 20:5 n-3). However, to our knowledge, little information is available regarding the physiological response of adult sea urchins to dietary ARA. In the present study, four dietary feeds were formulated with graded ARA(0, 0.5%, 1%, and 2% dry diet). Each diet was randomly allocated to three cages during a 56-day feeding experiment. The results showed that the sea urchin weight gain rate(WGR) and the gonadosomatic index(GI) significantly increased as ARA was equal to or above 1.0% of dry diet(P < 0.05). The activities of superoxide dismutase(SOD), catalase(CAT), and total anti-oxidative capacity(T-AOC) were the highest in the coelomic fluid of sea urchins that were fed diets with 1% ARA. The total essential amino acid(TEAA) and its ratio to total non-essential amino acid(TNEAA) showed a similar tendency to WGR and GI as dietary ARA increased, and the highest TEAA and TEAA/TNEAA were observed in the gonads of sea urchins that were fed diets with 1% ARA. Levels of ARA and ARA/EPA of the gonads increased while n-3/n-6 polyunsaturated fatty acid(PUFA) decreased with the increase of dietary ARA(P < 0.05). EPA in the gonads of experimental animals fed with formulated feeds showed no significant differences(P> 0.05), but was significantly lower than those fed with kelp(P < 0.05). These results suggested that relatively higher levels of ARA(1% dry diet) significantly promoted growth, gonad development, activities of antioxidant enzymes, as well as nutritional values(TEAA, TEAA/TNEAA, and PUFA) of adult S. intermedius.ZUO Rantao LI Min DING Jun CHANG Yaqing 2018Journal of Ocean University of China2018,17,4:3
2Higher dietary protein increases growth performance,anti-oxidative enzymes activity and transcription of heat shock protein 70 in the juvenile sea urchin(Strongylocentrotus intermedius)under a heat stress显示文摘This study was conducted to investigate the effect of dietary protein concentration(12%,18%,24%,30% and 36%)on the growth performance,activity of anti-oxidative enzymes and heat shock protein 70(HSP70)transcription in the sea urchin(Strongylocentrotus intermedius)under a heat stress.After 112 days of feeding trial the sea urchins were heat stressed(26C)and the coelomic fluid and intestine sampled at time 0 and 15 min,2 h and 6 h.The results showed that an increase in dietary protein(12%-24%),significantly increased(p<0.05)the sea urchin weight gain rate(WGR).As dietary protein increased(from 18% to 36%),the gonadosomatic index(GI)of juvenile sea urchins also significantly increased(p<0.05)from 18.0%to 22.6%.Superoxide dismutase(SOD)activity increased with dietary protein increase(12%-30%)and the enzyme activity was significantly higher(p<0.05)in the coelomic fluid of sea urchins that were fed with 30% diets when compared to 12% and 36% protein diets at all time points after the heat stress.Catalase(CAT)activity showed a similar tendency with the increase in dietary protein concentration at time 0 and 15 min after the heat stress(p<0.05).Transcription of HSP70 in the intestine also showed a similar trend to SOD and was highest in the animals that were fed with 30% protein diets(p<0.05).Our results suggest that 24% protein diets could meet the requirements for growth performance but a 30% protein diet resulted in improved gonad development and anti-heat stress capacity in this sea urchin species.Rantao Zuo Shouquan Hou Fanxiu Wu Jian Song Weijie Zhang Chong Zhao Yaqing Chang 2017Aquaculture and Fisheries2017,2,1:2
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